Fabiana Caetano

Fabiana Caetano
The University of Western Ontario | UWO · Department of Physiology and Pharmacology

PhD

About

31
Publications
3,119
Reads
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648
Citations
Citations since 2017
0 Research Items
376 Citations
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2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
Additional affiliations
August 2011 - present
The University of Western Ontario
Position
  • Laboratory and Facility Manager
August 2011 - present
The University of Western Ontario
Position
  • Facility Manager and Research Associate
Description
  • Training, implementation of new applications, customized assistance and management of all administrative aspects of a core imaging facility. As Research Associate, I study molecular basis of neurodegenerative diseases, more focused in Alzheimer's disease.
August 2011 - August 2015
The University of Western Ontario
Position
  • Confocal imaging Facility Manager, Research Associate

Publications

Publications (31)
Article
Full-text available
Fig. 6 was an inadvertent duplication of Fig. 8. This error has now been corrected. In Fig. 8B, the Akt immunoblot from wt VSMCs should be switched with the Akt immunoblot from shGRK2 VSMCs. These errors do not affect the results or conclusions of this work. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.
Article
Full-text available
The prion protein (PrPC) has been suggested to operate as a scaffold/receptor protein in neurons, participating in both physiological and pathological associated events. PrPC, laminin and metabotropic glutamate receptor 5 (mGluR5) form a protein complex on the plasma membrane that can trigger signalling pathways involved in neuronal differentiation...
Article
Full-text available
The M-transcript of human choline acetyltransferase (ChAT) produces an 82-kDa protein (82-kDa ChAT) that concentrates in nuclei of cholinergic neurons. We assessed the effects of acute exposure to oligomeric amyloid-β1–42 (Aβ1–42) on 82-kDa ChAT disposition in SH-SY5Y neural cells, finding that acute exposure to Aβ1–42 results in increased associat...
Article
Full-text available
Tip-enhanced Raman spectroscopy (TERS) provides greatly enhanced Raman signals along with ultrahigh lateral spatial resolution and has demonstrated to be a technique of choice to study a variety of biochemical specimens such as DNA and RNA at the single chain level. However, the sensitivity of TERS to demosntrate the influence of the nanoscale envi...
Article
Full-text available
The rise of molecular plasmonics and its application to ultrasensitive spectroscopic measurements has been enabled by the rational design and fabrication of a variety of metallic nanostructures. Advanced nano and microfabrication methods are key to the development of such structures, allowing one to tailor optical fields at the sub-wavelength scale...
Article
Full-text available
Our current understanding of the molecular mechanisms which regulate cellular processes such as vesicular trafficking has been enabled by conventional biochemical and microscopy techniques. However, these methods often obscure the heterogeneity of the cellular environment, thus precluding a quantitative assessment of the molecular interactions regu...
Data
Impact of partial clustering on RDF and Ripley’s H-function. Model of two co-clustered molecules (5,000/color) with an increasing portion of unclustered molecules. Each frame 200 additional unclustered molecules are added to each population (left panel). Upper-right panel display’s cross-Ripley’s H function for each frame, while the lower-right pan...
Data
Spatial association analysis–Operation and effect of over- and under-sampling. (A) Spatial association analysis functions by assessing distances between the molecule of interest (blue circles) and the nearest-neighbour molecule in the other colour channel (red circles). The intermolecular separation distances between nearest neighbours is calculate...
Data
Evolution of RDF and Ripley’s H-function curves with increasing clustering of two initially unclustered populations. Model of two randomly distributed populations of 5,000 molecules/population (red and blue dots, left panel) which coalesce into 50 co-clusters. Upper-right panel display’s cross-Ripley’s H function for each frame, while the lower-rig...
Data
Radial distribution function–Operation and effect of over- and under-sampling. (A) RDF (pair-correlation or G(r)) analysis operates by quantifying the number of molecules (blue points) in an expanding torus centered on each molecule, normalized to the average density of molecules in the sample. For an unclustered dataset, G will have a value of 1 a...
Data
Ripley’s K-function–Operation and effect of over- and under-sampling. (A) Ripley’s K function operates by quantifying the density of particles (blue points) in an expanding circular area around each point, compared to the number of points predicted given the average point density of the image. When K is plotted against radius, clustering appears as...
Data
Evolution of RDF and Ripley’s H-function curves with increasing clustering of a random population with a pre-clustered population. Model of one randomly distributed population of 5,000 molecules (blue dots) which coalesce into 50 pre-existing clusters of red molecules. Upper-right panel display’s cross-Ripley’s H function for each frame, while the...
Data
Operation of the DBSCAN and OPTICS algorithms. (A) The DBSCAN algorithm requires the user to provide the minimum number of particles in a cluster (k) and the neighbourhood size in which a cluster would exist (ε). Assuming k = 5, molecule ‘a’ (green) would be considered directly density reachable, and thus a core molecule in a cluster, as more than...
Data
MIiSR program. This zip-compressed archive contains the MIiSRconvert and MIiSR GUI’s, as well as stand-alone function for scripts for converting common super-resolution microscopy position files to Matlab-compatible matrices (fileConv.m), for cropping and filtering super-resolution microscopy position files (LoadCrop.m), and to perform the SAA (SAA...
Data
Derivation of the SAA, RDF, Ripley’s, DBSCAN and OPTICS algorithm. Complete derivation of all algorithms used in the MIiSR toolbox. (DOCX)
Article
Full-text available
Background Agonist stimulation of Group I metabotropic glutamate receptors (mGluRs) initiates their coupling to the heterotrimeric G protein, Gαq/11, resulting in the activation of phospholipase C, the release of Ca2+ from intracellular stores and the subsequent activation of protein kinase C. However, it is now recognized that mGluR5a also functio...
Article
Full-text available
Hypertension, elevated arterial pressure, occurs as the consequence of increased peripheral resistance. G protein-coupled receptors (GPCRs) contribute to the regulation of vasodilator and vasoconstrictor responses and their activity is regulated by a family of GPCR kinases (GRKs). GRK2 expression is increased in hypertension and this facilitates th...
Article
Full-text available
Serotonin (5-HT) interacts with a wide variety of 5-HT receptors (5-HTR) of which 5-HT2AR plays an important target for antidepressant and atypical antipsychotic drugs. The carboxyl-terminal tail of 5-HT2AR encodes a motif that mediates interactions with PSD-95/Discs Large/Zona Occludens 1 (PDZ) domain containing proteins. In the present study, we...
Article
Full-text available
Stress-inducible phosphoprotein 1 (STI1), a co-chaperone for Hsp90, has been shown to regulate multiple pathways in astrocytes, but its contributions to cellular stress responses are not fully understood. We show that in response to irradiation-mediated DNA damage stress STI1 accumulates in the nucleus of astrocytes. Also, STI1 haploinsufficiency d...
Chapter
Stroke is followed by a dramatic increase in intracellular calcium. This excessive increase in calcium levels is critical in the initiation of neuronal cell death. N-Methyl-d-aspartate (NMDA) receptor and L-type voltage-dependent calcium channel antagonists were unsuccessful at providing neuroprotection following ischemia in clinical trials. Theref...
Article
Full-text available
J. Neurochem. (2011) 117, 538–553. In Alzheimer’s disease, the amyloid-β peptide (Aβ) interacts with distinct proteins at the cell surface to interfere with synaptic communication. Recent data have implicated the prion protein (PrPC) as a putative receptor for Aβ. We show here that Aβ oligomers signal in cells in a PrPC-dependent manner, as might b...
Article
Full-text available
The prion protein (PrP(C)) is highly expressed in the nervous system, and its abnormal conformer is associated with prion diseases. PrP(C) is anchored to cell membranes by glycosylphosphatidylinositol, and transmembrane proteins are likely required for PrP(C)-mediated intracellular signaling. Binding of laminin (Ln) to PrP(C) modulates neuronal pla...
Article
Full-text available
The secreted cochaperone STI1 triggers activation of protein kinase A (PKA) and ERK1/2 signaling by interacting with the cellular prion (PrP(C)) at the cell surface, resulting in neuroprotection and increased neuritogenesis. Here, we investigated whether STI1 triggers PrP(C) trafficking and tested whether this process controls PrP(C)-dependent sign...
Article
Beta-hexabromo-5,10,15,20-tetrakis(4-carbomethoxyphenyl)porphyrinatomanganese(III) chloride (Mn(III)(Br6TCMPP)Cl) was prepared by selective Br2-hexabromation of its parent non-brominated manganese complex (Mn(III)(TCMPP)Cl), whereas the octabrominated analogue beta-octabromo-5,10,15,20-tetrakis(4-carbomethoxyphenyl)porphyrinatomanganese(III) chlori...

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