F. Javier Lopez-Jaramillo

F. Javier Lopez-Jaramillo
University of Granada | UGR · Department of Organic Chemistry

PhD

About

103
Publications
24,859
Reads
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2,433
Citations
Additional affiliations
April 2009 - December 2016
University of Granada
Position
  • Professor (Associate)
March 2007 - October 2007
Universidad de Jaén
Position
  • Lecturer
January 2005 - March 2009
University of Granada
Position
  • Research Associate
Description
  • Bioconjugation, Drug delivery, Nonviral Gene Delivery Vectors, Carbohydrate-protein interaction

Publications

Publications (103)
Article
Full-text available
Water-insoluble carbon dots are recognized as promising materials, although their applications in nanomedicine are rarely explored, despite their lipophilic character and foreseen compatibility with biological membranes. In this article, we exploit the anhydride functionalization of carbon dots obtained by thermolysis of citric acid to synthesize a...
Article
Full-text available
Molecular fluorophores of diverse nature are a predominant component and a source of the fluorescence in carbon nanodots (CNDs) obtained by thermal procedures. Here, we report on the implementation of the fluorescence properties of 5-oxo-1,2,3,5-tetrahydroimidazo[1,2-a]pyridine-7-carboxylic acid (IPCA), the most paradigmatic of the molecular fluoro...
Article
Full-text available
Activated carbon nanodots functionalized with acid anhydride groups (AA-CNDs) are prepared by the one-pot water-free green thermolysis of citric acid. As a proof of concept of their capabilities as appealing and versatile platforms for accessing engineering nanoconstructs, the as-prepared AA-CNDs have been reacted to yield clickable CNDs. Their cli...
Article
Full-text available
Magnetite nanoparticles (MNPs) coated by branched poly (ethylene-imine) (PEI) were synthesized in a one-pot. Three molecular weights of PEI were tested, namely, 1.8 kDa (sample MNP-1), 10 kDa (sample MNP-2), and 25 kDa (sample MNP-3). The MNP-1 particles were further functionalized with folic acid (FA) (sample MNP-4). The four types of particles we...
Article
Full-text available
The easy functionalization of tags and solid supports with the vinyl sulfone function is a valuable tool in omic sciences that allows their coupling with the amine and thiol groups present in the proteogenic residues of proteins, in mild and green conditions compatible with their biological function.
Article
Full-text available
Heat stress is one of the abiotic stresses that leads to oxidative stress. To protect themselves, yeast cells activate the antioxidant response, in which cytosolic peroxiredoxin Tsa1 plays an important role in hydrogen peroxide removal. Concomitantly, the activation of the heat shock response (HSR) is also triggered. Nitro-fatty acids are signaling...
Article
Full-text available
Nitric oxide (NO) is a free radical which modulates protein function and gene expression throughout all stages of plant development. Fruit ripening involves a complex scenario where drastic phenotypical and metabolic changes take place. Pepper fruits are one of the most consumed horticultural products worldwide which, at ripening, undergo crucial p...
Article
The use of the specific binding properties of monoclonal antibody fragments such as single-chain variable fragments (ScFv) for the selective delivery of antitumor therapeutics for cancer cells is attractive due to their smaller size, low immunogenicity, and low-cost production. Although covalent strategies for the preparation of such ScFv-based the...
Article
Full-text available
Catalase is a powerful antioxidant metalloenzyme located in peroxisomes which also plays a central role in signaling processes under physiological and adverse situations. Whereas animals contain a single catalase gene, in plants this enzyme is encoded by a multigene family providing multiple isoenzymes whose number varies depending on the species,...
Article
Full-text available
Low temperature (LT) negatively affects plant growth and development via the alteration of the metabolism of reactive oxygen and nitrogen species (ROS and RNS). Among RNS, tyrosine nitration, the addition of an NO2 group to a tyrosine residue, can modulate reduced nicotinamide-dinucleotide phosphate (NADPH)-generating systems and, therefore, can al...
Article
Like nitric oxide (NO), hydrogen sulfide (H⁠2S) has been recognized as a new gasotransmitter which plays an important role as a signaling molecule in many physiological processes in higher plants. Although fruit ripening is a complex process associated with the metabolism of reactive oxygen species (ROS) and nitrogen oxygen species (RNS), little is...
Research
Structure deposited in PDB with code 1ZIS
Article
Full-text available
Saponins are potential wide-spectrum antitumor drugs, and copper(I) catalyzed azide− alkyne 1,3-dipolar cycloaddition is a suitable approach to synthesizing saponinlike compounds by regioselective glycosylation of the C2/C3 hydroxyl and C28 carboxylic groups of triterpene aglycones maslinic acid (MA) and oleanolic acid (OA). Biological studies on t...
Article
Full-text available
Saponins are potential wide-spectrum antitu- mor drugs, and copper(I) catalyzed azide−alkyne 1,3-dipolar cycloaddition is a suitable approach to synthesizing saponin- like compounds by regioselective glycosylation of the C2/C3 hydroxyl and C28 carboxylic groups of triterpene aglycones maslinic acid (MA) and oleanolic acid (OA). Biological studies o...
Article
Full-text available
Gold nanoparticles (AuNPs) can be obtained from HAuCl4 by using polyethyleneimine (PEI) as both reductant and stabilizing agent. However, the known affinity of PEI for different materials has not been exploited to coat them and turn their surface into catalytic. We demonstrate that the irradiation of a solution of HAuCl4 and branched PEI 1800 (bPEI...
Article
Full-text available
Despite the importance of the adjuvant in the immunization process, very few adjuvants merge with the antigens in vaccines. A synthetic self-adjuvant oleic-vinyl sulfone (OVS) linked to the catalytic region of recombinant serine/threonine phosphatase 2A from the nematode Angiostrongylus costaricensis (rPP2A) was used for intranasal immunization in...
Data
Table S1 from Self-adjuvanting C18 Lipid Vinylsulfone-PP2A vaccine: study of the induced immunomodulation against Trichuris muris infection by M. Gomez-Samblas, JJ. García-Rodríguez, M Trelis, D. Bernal, FJ Lopez-Jaramillo, F Santoyo-Gonzalez, S. Vilchez, AM Espino, F. Bolás-Fernández, A. Osuna; Table S2 from Self-adjuvanting C18 Lipid Vinylsulfone...
Article
The term coupling-and-decoupling (CAD) chemistry refers to applications in which efficient bond formation and subsequent cleavage between two moieties is required. Within this context, the scope of the vinyl sulfonate (VSO) group as an effi- cient tool for CAD chemistry is reported. The cou- pling step relies on the click features of the Michael- t...
Cover Page
Full-text available
The front cover image illustrates the new vinyl sulfonate-based “click” Coupling-and-Decoupling methodology by Victor Blanco, Francisco Santoyo-Gonzalez and co-workers. The coupling step relies on the click Michael-type addition of nucleophiles to vinyl sulfonates, while the decoupling involves the cleavage of the resulting sulfonate adducts either...
Article
Full-text available
Background Around 20%–30% of breast cancers overexpress the proto-oncogene human epidermal growth receptor 2 (HER2), and they are characterized by being very invasive. Therefore, many current studies are focused on testing new therapies against tumors that overexpress this receptor. In particular, there exists major interest in new strategies to fi...
Article
A novel one-pot methodology for the synthesis of PEI-coated gold nanoparticles (AuPEI-NPs) that combines the reductant-stabilizer properties of PEI and microwave irradiation starting from hydrogen tetrachloroaurate (HAuCl4) and branched PEI 25kDa (b25kPEI) is reported. The method is straightforward, green and low-cost where the Au:PEI w:w ratio (1:...
Patent
ES 2 579 487 The present invention relates to the use of cross-linked saccharide-based polymers as bile acid sequestrants
Article
Full-text available
Gene transfection mediated by the cationic polymer polyethylenimine (PEI) is considered a standard methodology. However, while highly branched PEIs form smaller polyplexes with DNA that exhibit high transfection efficiencies, they have significant cell toxicity. Conversely, low molecular weight PEIs (LMW-PEIs) with favourable cytotoxicity profiles...
Article
Full-text available
The cytotoxicity of 27 benzanilides and dithiobenzanilides built on a stilbene scaffold and possessing various functional groups in aromatic rings previously described for their spasmolytic properties was assayed on three human cancer cell lines (A549 -lung adenocarcinoma, MCF-7 estrogen dependent breast adenocarcinoma and MDA-MB-231 estrogen indep...
Article
Full-text available
The ascorbate–glutathione cycle is a metabolic pathway that detoxifies hydrogen peroxide and involves enzymatic and non-enzymatic antioxidants. Proteomic studies have shown that some enzymes in this cycle such as ascorbate peroxidase (APX), monodehydroascorbate reductase (MDAR), and glutathione reductase (GR) are potential targets for post-translat...
Article
Full-text available
The aim of this study was to evaluate the crosslinking abilities of divinyl sulfone (DVS) for the preparation of novel water-insoluble cyclodextrin-based polymers (CDPs) capable of forming inclusion complexes with different guest molecules. Reaction of DVS with native α-cyclodextrin (α-CD), β-cyclodextrin (β-CD) and/or starch generates a variety of...
Article
Full-text available
Bile acid sequestrants (BAS) represent a therapeutic approach for the management of hypercholesterolemia that relies on the cationic polymeric nature of BAS to selectively bind negatively charged bile acids. We hypothesized that the cross-linking of β-cyclodextrin (β-CD) and saccharides such as starch or dextrin with divinyl sulfone (DVS) yields ho...
Conference Paper
Full-text available
Among the different strategies to deliver drugs to specific physiological sites where pharmacological action is required, active targeting is an appealing approach that relies on the use of specific interactions with the target to direct and concentrate the drug at the site of action. The coupling of the therapeutic agent to the targeting agent and...
Article
Full-text available
The receptor for advanced glycation end products (RAGE) is involved in diabetes or angiogenesis in tumors. Under pathological conditions, RAGE is overexpressed and upon ligand binding and internalization stimulates signaling pathways that promote cell proliferation. In this work, amino dendritic polymers PEI 25 kDa and alkylated derivatives of PAMA...
Article
Cyclodextrins have been conjugated to target various receptors and have also been functionalized with carbohydrates for targeting specific organs. However, this approach is based on a rigid design that implies the ad hoc synthesis of each cyclodextrin-targeting agent conjugate. We hypothesized that: 1) a modular design that decouples the carrier fu...
Article
Full-text available
Post-translational modifications (PTMs) mediated by nitric oxide (NO)-derived molecules have become a new area of research, as they can modulate the function of target proteins. Proteomic data have shown that ascorbate peroxidase (APX) is one of the potential targets of PTMs mediated by NO-derived molecules. Using recombinant pea cytosolic APX, the...
Article
The chemical behavior of S-glycopyranosyl-N-monoalkyl dithiocarbamates (DTCs) as masked 1-glycosyl thiols, easily prepared by the nucleophilic displacement of 1-halo sugars with dithiocarbamate salts of primary amines, has been studied and synthetically exploited. This behavior relies on the abstraction of the proton of the carbamate functionality...
Article
Protein tyrosine nitration is a post-translational modification (PTM) mediated by nitric oxide-derived molecules. Peroxisomes are oxidative organelles in which the presence of nitric oxide (NO) has been reported. We studied peroxisomal nitroproteome of pea leaves by high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) and...
Article
Full-text available
Background S-nitrosylaton is implicated in the regulation of numerous signaling pathways with a diversity of regulatory roles. The high lability of the S-NO bond makes the study of proteins regulated by S-nitrosylation/denitrosylation a challenging task and most studies have focused on already S-nitrosylated proteins. We hypothesize that: i) S-nitr...
Conference Paper
Full-text available
Among the different post-translational modifications of proteins, glycosylation is the most prevalent one, occurring in at least 50% of all proteins, and carbohydrate-mediated molecular recognition is involved in many biological aspects. However, carbohydrate research has to face several challenges. Thus, array technology is a convenient approach t...
Article
Carbohydrate-mediated molecular recognition is involved in many biological aspects such as cellular adhesion, immune response, blood coagulation, inflammation, and infection. Considering the crucial importance of such biological events in which proteins are normally involved, synthetic saccharide-based systems have emerged as powerful tools for the...
Article
Full-text available
High temperature (HT) is considered a major abiotic stress that negatively affects both vegetative and reproductive growth. Whereas the metabolism of reactive oxygen species (ROS) is well established under HT, less is known about the metabolism of reactive nitrogen species (RNS). In sunflower (Helianthus annuus L.) seedlings exposed to HT, NO conte...
Article
A new approach for the preparation of carbohydrate-coated magnetic nanoparticles is reported. In a first step, we show that the pH-driven assembly-disassembly natural process that occurs in apoferritin protein is effective for the encapsulation of maghemite nanoparticles of different sizes: 4 and 6 nm. In a second step, we demonstrate that the pres...
Article
High temperature (HT) is considered a major abiotic stress that negatively affects both vegetative and reproductive growth. Whereas the metabolism of reactive oxygen species (ROS) is well established under HT, less is known about the metabolism of reactive nitrogen species (RNS). In sunflower (Helianthus annuus L.) seedlings exposed to HT, NO conte...
Article
Novel supported chelating adsorbents bearing diverse multidentate nitrogenated ligands with strong copper(I) affinities are easily prepared in non-magnetic and magnetic variants using silica and silica-coated magnetite nanoparticles as suitable supports and the aza-Michael-type addition of vinyl sulfones as the ligation tool. These adsorbents are v...
Article
Full-text available
The combination of silica as support and vinyl sulfone as reactive group led to a pre-activated material that readily reacts to form covalent bonds by Michael-type addition with both amine and thiol groups naturally occurring in biomolecules in mild conditions compatible with the biological nature of the enzymes. A simple two step synthetic strateg...
Article
The introduction of multiple labels onto biomolecules is a challenge. We report herein the synthesis of vinyl sulfone derivatized bifunctional tag single-attachment-point reagents (BTSAP) bearing biotin and a fluorescent tag and their applications in proteins for the introduction of multiple labels by means of the Michael-type addition of the elect...