Ewa Moczko

Universidad Adolfo Ibáñez · Faculty of Engineering and Sciences

Human performance enhancing drugs (PEDs), frequently used in sport competitions, are strictly prohibited by the World Anti-Doping Agency (WADA). Biological samples collected from athletes and regular patients are continuously tested regarding the identification and/or quantification of the banned substances. Current work is focused on the applicati...

The bark of Pinus radiata D. Don is a bioresource of great worldwide abundance. While various forms of use have been studied, it is still a little-used bioresource. Due to its great accumulation, significant solid emissions of this residue generate environmental problems such as changes in soil chemistry, ecological problems such as the alteration...

Molecularly imprinted polymer nanoparticles (nanoMIPs) are receiving broad interest as robust and highly selective synthetic receptors for a variety of molecules. Due to their stability, inexpensive synthesis and easy implementation, they are considered a promising alternative to antibodies in sensors, diagnostics and separation applications. The m...

In 2004, octopamine was added to the list of drugs banned by the world anti-doping agency (WADA) and prohibited in any sport competition. This work aims to develop a new analytical method to detect octopamine in water and human urine samples. We proposed a pseudo-enzyme-linked immunosorbent assay (pseudo-ELISA) by replacing traditional monoclonal a...

The authors wish to make the following erratum to this paper [...]

It has been shown that the faradic current at an electrode grafted with molecularly imprinted polymer (MIP) is sensitive to the specific target molecule used as the template. This phenomenon is applicable to sensors with very high selectivity, but the sensing mechanism is still a black box. We investigated the size sensitivity of nanoparticles of m...

This paper describes the development of a novel sorbent for selective extraction of endocrine disruptors (EDs) from aqueous media. The main goal was to obtain sufficient molecularly imprinted polymers (MIPs) for selective detection, preconcentration, and extraction of EDs such as bisphenol A (BPA) and progesterone (PG). Series of MIPs and their ana...

Herein we describe the preparation of molecularly imprinted silica nanoparticles by Ostwald ripening in the presence of molecular templates immobilised on glass beads (the solid-phase). To achieve this, a seed material (12 nm diameter silica nanoparticles) was incubated in phosphate buffer in the presence of the solid-phase. Phosphate ions act as a...

Our study involves development of fluorescent cell-based diagnostic assay as a new approach in high-throughput screening method. This highly sensitive optical assay operates similarly to e-noses and e-tongues which combine semi-specific sensors and multivariate data analysis for monitoring biochemical processes. The optical assay consists of a mixt...

Cooperative binding is commonly observed in biological receptor systems. This study investigates whether it is possible to prepare nano-sized molecularly imprinted polymers (nanoMIPs) that show cooperative binding. NanoMIPs which exhibit cooperative binding would have increased affinity for immobilised template molecules making them useful for adva...

The aim of this work is to evaluate whether the size of the analyte used as template for the synthesis of molecularly imprinted polymer nanoparticles (nanoMIPs) can affect their performance in pseudo-enzyme linked immunosorbent assay (pseudo-ELISA). Successful demonstration of a nanoMIPs based pseudo-ELISA for vancomycin (1449.3 g mol-1) was demons...

Curcumin is a versatile anti-inflammatory and anti-cancer agent known for its low bioavailability, which could be improved by developing materials capable of binding and releasing drug in a controlled fashion. The present study describes the preparation of magnetic nano-sized Molecularly Imprinted Polymers (nanoMIPs) for the controlled delivery of...

A new ELISA- (enzyme-linked immunosorbent assay)-like assay is demonstrated in which no elements of biological origin are used for molecular recognition or signaling. Composite imprinted nanoparticles that contain a catalytic core and which are synthesized by using a solid-phase approach can simultaneously act as recognition/signaling elements, and...

Molecularly imprinted polymer nanoparticles (MIP NPs) are potential generic alternatives to traditional receptors such as antibodies, proteins and enzymes in diagnostic, separation and drug development. Like their natural counterparts, MIP NPs exhibit high affinity and specificity for their targets but possess higher stability, lower manufacturing...

A simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanoMIPs) to develop ELISA type assays is presented here for the first time. NanoMIPs were synthesized by a solid phase approach with immobilized vancomycin (template) and characterized using Biacore 3000, dynamic light scattering a...

An application of plasma-induced grafting of polyethylene membranes with a thin layer of molecularly imprinted polymer (MIP) was presented. High-density polyethylene (HDPE) membranes, "Vyon," were used as a substrate for plasma grafting modification. The herbicide atrazine, one of the most popular targets of the molecular imprinting, was chosen as...

The synthesis of core-shell molecularly imprinted polymer nanoparticles (MIP NPs) has been performed using a novel solid-phase approach on immobilised templates. The same solid phase also acts as a protective functionality for high affinity binding sites during subsequent derivatisation/shell formation. This procedure allows for the rapid synthesis...

This work presents the development of molecularly imprinted polymers (MIPs) for the selective extraction of dimethoate from olive oil. Computational simulations allowed selecting itaconic acid as the monomer showing the highest affinity towards dimethoate. Experimental validation confirmed modelling predictions and showed that the polymer based on...

A specific adsorbent for extraction of methidathion from olive oil was developed. The design of the molecularly imprinted polymer (MIP) was based on the results of the computational screening of the library of polymerisable functional monomers. MIP was prepared by thermal polymerisation using N,N'-methylene bisacrylamide (MBAA) as a functional mono...

Poly(3,4-ethylenedioxythiophene) (PEDOT) and its derivatives are relatively new, and unique members of conducting-polymers family. In this article, we present an approach for simple, reliable and cost-efficient electrochemical biosensor for real-time detection and quantification of phenolic compounds (PhCs). The PEDOT:poly(styrene sulfonate) (PSS)...

In this paper, we present an optical diagnostic assay consisting of a mixture of environmental-sensitive fluorescent dyes combined with multivariate data analysis for quantitative and qualitative examination of biological and clinical samples. The performance of the assay is based on the analysis of spectrum of the selected fluorescent dyes with th...

A combination of selective fluorescent dyes has been developed for simultaneous quantitative measurements of several physicochemical parameters. The operating principle of the assay is similar to electronic nose and tongue systems, which combine nonspecific or semispecific elements for the determination of diverse analytes and chemometric technique...

Currently, precise non-invasive diagnostics systems for the real-time multi detection and monitoring of physiological parameters and chemical analytes in the human body are urgently required by clinicians, physiologists and bio-medical researchers. We have developed a novel cost effective smart 'vanishing tattoo' (similar to temporary child's tatto...

Currently, precise non-invasive diagnostics systems for the real-time multi detection and monitoring of physiological parameters and chemical analytes in the human body are urgently required by clinicians, physiologists and bio-medical researchers. We have developed a novel cost effective smart 'vanishing tattoo' (similar to temporary child's tatto...