Ewa Kłodzińska

• PhD., DSc , Prof. IS- PIB
• Head of Department at Instiute of Sport - National Research Institute, Poland

Strategies for successful healing of diabetic wounds are lacking. Natural-derived dressings have gained much attention due to their promising properties. We hypothesized that keratin-biphalin fiber-dressing accelerates skin wound healing in a mouse model of diabetes. Keratin fibers were obtained from mouse fur and soaked in a biphalin solution. Stu...

In modern medical diagnostics, where analytical chemistry plays a key role, fast and accurate identification of pathogens is becoming increasingly important. Infectious diseases pose a growing threat to public health due to population growth, international air travel, bacterial resistance to antibiotics, and other factors. For instance, the detecti...

The present study attempted to apply the capillary electrophoresis technique for the fractionation and separation of S. Staphylococcus hominis and Escherichia coli bacteria isolated from urine samples and the detection of migrated fraction with spectrometric method. This involved the selection of suitable conditions for separation as well as the id...

Bisphenol A (BPA) and its analogues showing structural and functional similarity to BPA are commonly applied in various industrial applications and thus are becoming ubiquitous in the environment. At the same time there is increasing scientific evidence that exposure to these chemicals may lead to adverse health effects in human and wildlife. In re...

Abstract: In competitive athletes, the differential diagnosis between nonpathological changes in cardiac morphology associated with training (commonly referred to as “athlete’s heart”) and certain cardiac diseases with the potential for sudden death is an important and not uncommon clinical problem. The use of noninvasive, fast, and cheap analytica...

Nickel nanoparticles (Ni NPs) are utilized extensively in various industrial applications. However, there are increasing concerns about potential exposure to Ni NPs and consequent health effects. The aim of this study was to assess Ni NPs-induced liver toxicity in Sprague Dawley rats. Twenty-five rats were exposed to Ni NPs via intraperitoneal inje...

The inflammatory phase, which begins in the healing process right after the hemostasis phase, is basically a prelude to the actual healing process. However, an excessive or too long inflammatory phase can lead to impaired healing and the formation of non-healing wounds. On the other hand, the most important from the point of view of biomedical engi...

Impaired wound healing is a major medical challenge, especially in diabetics. Over the centuries, the main goal of tissue engineering and regenerative medicine has been to invent biomaterials that accelerate the wound healing process. In this context, keratin-derived biomaterial is a promising candidate due to its biocompatibility and biodegradabil...

Fast determination, identification and characterization of pathogens is a significant challenge in many fields, from industry to medicine. Standard approaches (e.g., culture media and biochemical tests) are known to be very time-consuming and labor-intensive. Conversely, screening techniques demand a quick and low-cost grouping of microbial isolate...

Bromhexine (BH), expectorant used in the treatment of respiratory disorders associated with viscid or excessive mucus, is not permitted for use in the competing horse by many authorities in horseracing and Olympic disciplines. Metabolic studies are of the great importance in anti-doping field because they allow for updating the selection of the mos...

Keratin is a cytoskeletal scaffolding protein essential for wound healing and tissue recovery. The aim of the study was to evaluate the potential role of insoluble fur keratin-derived powder containing silver nanoparticles (FKDP-AgNP) in the allogenic full-thickness surgical skin wound model in diabetic mice. The scanning electron microscopy image...

Continuous research on metabolomics is of utmost importance in the field of anti-doping control, including updating the selection of the most appropriate markers of prohibited substances, testing specimens and analytical strategies. Among metabolomics research topics, nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used in equine medicine...

Ibuprofen (IBU) is a widely used nonsteroidal anti-inflammatory drug (NSAID) in human and also veterinary medicine. NSAIDs are usually highly metabolized compounds in horses; they are often present in equine urine, mostly in their conjugated forms (glucuronides). Thus, hydrolysis to cleave the glucuronide linkage prior to anti-doping analysis is of...

Gamma-oryzanol (GO) has gained special attention in the equine sports industry in recent years due to its touted properties, including the fact that it may cause anabolic effects on muscle growth and reduce fatigue. Many manufactures offer supplements containing GO as a naturally occurring anabolic substance; however, some producers do not declare...

The recent emergence of nanotechnology has provided a new therapeutic modality in case of silver nanoparticles. Dressings containing silver form the basis for the treatment of burns and wounds, either acute or chronic ones. The aim of the study was to examine silver release from the different wound dressings: commercially available (Atrauman Ag, Aq...

In this study, GC‐MS‐ and MEKC‐based methods for determination of caffeine (CAF) in pre‐workout supplements were developed and validated. The proposed protocols utilized minimal sample preparation (simple dilution and syringe filtration). The developed methods achieved satisfactory validation parameters, i.e. good linearity (R²>0.9988 and R²>0.9985...

The reports on the probable beneficial impact of tyrosine (TYR) supplementation on performance enhancement have contributed to the growth of interest in TYR in equestrian sports field and related industries, such as the manufacture of dietary and nutritional supplements. In this study, the first attempt to the assessment of horses exposure to TYR d...

Impaired wound healing is a major medical problem in diabetes. The objective of this study was to determine the possible application of an insoluble fraction of fur-derived keratin biomaterial as a wound dressing in a full thickness surgical skin wound model in mice (n=20) with iatrogenically induced diabetes. The obtained keratin dressing was exam...

Two novel complementary analytical methods, namely an extraction-free capillary electrophoresis coupled to mass spectrometry (CE-MS) and direct immersion-solid-phase microextraction combined with gas chromatography-mass spectrometry (DI-SPME-GC-MS), have been developed and successfully applied for the determination of biogenic amines (BAs) in wine...

Ibuprofen is widely used in human and veterinary medicine for the treatment of chronic pain as well as rheumatic and musculoskeletal disorders. However, the analgesic and anti‐inflammatory properties of Ibuprofen have contributed to frequent drug abuse in equestrian sports. A sensitive and rapid GC–MS/MS‐based method with a simple liquid‐liquid ext...

Biogenic amines (BAs) have been reported in a variety of foods, such as fish, meat, cheese, and wines. The formation of BAs in food by the microbial decarboxylation of amino acids can result in human allergic reactions, characterized by difficulty in breathing, rash, vomiting, and hypertension. Control measures to prevent biogenic amine formation i...

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Wstęp: Jednym z celów inżynierii tkankowej i medycyny regeneracyjnej jest poszukiwanie nowych sposobów opatrywania ran. W tego typu schorzeniach materiały na bazie keratyny, stały się interesującym kandydatem ze względu na ich biozgodność, biodegradowalność oraz zdolność do wspierania wzrostu m.in. keratynocytów, fibroblastów. Cel badania: Wpływ o...

Micellar Electrokinetic Chromatography (MEKC)

This book has an important role in advancing non-classical materials on the macro and nanoscale. The book provides original, theoretical, and important experimental results. Some research uses non-routine methodologies often unfamiliar to some readers. Furthermore, papers on novel applications of more familiar experimental techniques and analyses o...

This review article focuses on the utilization of the electromigration techniques in determination of microorganisms and shows the applicational possibilities of these techniques mainly for medical purpose. First it describes the origin of bacterial surface charge and the impact of functional groups on bacterial properties measured by hyphenated te...

Biodegradable polymers have gained increasing interest in recent years, especially in food packaging industry. The biodegradation process is intrinsically related to adhesion of degradation-promoting bacteria on the surface of a biodegradable packaging. On the other hand, because of a direct contact with food, these materials should be resistant to...

This new book will cover a broad range of polymeric materials and textiles and provides industry professionals and researchers in polymer science and technology with a single, comprehensive book summarizing all aspects involved in the functional materials production chain. This volume presents the latest developments and trends in advanced poly...

Breath analysis for the purpose of non-invasive diagnosis of lung cancer has yielded numerous candidate compounds with still questionable clinical relevance. To arrive at suitable volatile organic compounds our approach combined the analysis of different sources: isolated tumor samples compared to healthy lung tissues, and exhaled breath from lung...

This book provides a comprehensive presentation of the concepts, properties, and applications of classical materials. It also provides the first unified treatment for the broad subject of classical materials. The authors use a fundamental approach to define the structure and properties of a wide range of solids on the basis of the local chemical bo...

A surgical site infection (SSI) is an infection that occurs after surgery in the part of the body where the surgery took place. A SSI may range from a spontaneously limited wound discharge within 7-10 days of an operation to a life-threatening postoperative complication, such as a sternal infection after open heart surgery. Most surgical site infec...

The results of the transesterification methyl ester 3-(3', 5'-di-tert.butyl-4'- hydroxyphenyl)-propionic acid, are known and generalised by polyols in reviews [1]. However, the information bound to pentaerythrityl-tetrakis-(3-(3', 5'-di-tert.butyl-4'-hydroxyphenyl)-propionate) is discordant and mainly based on declaring of the patent data [2-7]. Th...

Ultimate strength, softening temperature, and water absorption of the polymer composites based on epoxy resin (type ED-20) with unmodified and/or modified by tetraethoxysilane (TEOS) minerals diatomite and andesite are described. Comparison of experimental results obtained for investigated composites shows that the ones containing modified filler h...

The chemical compounds, which are present in the environment, increasingly cause bad effects on health. The most serious effects are tumors and various mutations at the cellular level. Such compounds, from the analytical point of view, can serve the function of biomarkers, constituting measurable changes in the organism’s cells and biochemical proc...

Fundamentals on the measurement of the zeta potential for polymeric materials were given. Methods for studying solid surfaces and detn. of the zeta potential at the interface polymer/electrolyte soln. were reviewed.

In recent time the mineral fillers attract attention as active filling agents in polymer composites [1, 2]. Thanks to these fillers many properties of the composites are improved increases the durability and rigidity, decrease the shrinkage during hardening process and water absorption, improves thermal stability, fire proof and dielectric properti...

To assess the bacteria-antibiotic interactions in patients with postoperative wound infections, a simple electrophoretic test was performed. To estimate the effectiveness of the antibiotic therapy and to prepare 3-day profiles of bacteria "quantity" in biological samples, CE was used. As our team demonstrated earlier, the method is easy and fast, s...

Simple quantitative tests were performed to assess the viability of bacteria cells during capillary electrophoretic process. The results have shown that Gram-negative E. coli cells were more sensitive to electric field treatment in comparison to Gram-positive S. aureus. In comparison to a reference sample (0 kV) only 3.4% and 0.7% of the E. coli ce...

ABSTRACT: The basic clinical problem associated with infection treatment is the fact that classic, commonly and routinely used isolation and identification methods are based on long-term processes of a phenotypic analysis of microorganisms. Consequently sometimes, especially in small centres, rapid implementation of antibacterial treatment becomes...

Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause...

Electromigration techniques have been recently proposed as a replacement for traditional methods of microbial identification. The results of the initial experiments indicate that diagnostics based on this technology can be extremely sensitive, inexpensive and, primarily, very fast. Capillary electrophoresis is a novel method of chemical analysis wh...

The aggregation and/or adhesion of bacterial cells is a serious disadvantage of electrophoretic separations. In this study, physicochemical surface characteristics of bacteria were measured to establish their role in bacterial adhesion and aggregation on the basis of electrophoretic behavior of different clinical strains of Gram-positive Staphyloco...

The separation of bacteria by electromigration techniques was a subject of several of our previous papers. This contribution presents the results of investigation of the porosity of the monolithic bed and migration of Staphylococcus aureus cells through it. The gigaporous monolith was thermally synthesized using glycidyl methacrylate, triethylene g...

Staphylococcus aureus is a common cause of infection in both hospitals and the community, and it is becoming increasingly virulent and resistant to antibiotics. Possibilities of fast, sensitive and cheap determination of these pathogenic bacteria are extremely important in antimicrobial therapy. In the present study, CE with chemically modified cap...

Electrophoretic separation of bacteria as well as potential applications of electrophoresis in microbiological medical analysis has gained the interest of several research groups. The undesired effects such as aggregation and/or adhesion of the cells have a strong effect on the separation process. For a better understanding of the phenomena we deci...

Achievements in bacteria analysis with electromigration techniques may improve medical diagnoses, detection of food contamination, and sterility testing. (To listen to a podcast about this feature, please go to the Analytical Chemistry website at pubs.acs.org/ac .).

The importance of electromigration techniques in molecular biology and medicine is increasing rapidly, especially in systematic studies on proteomes and metabolomes. Staphylococcus aureus and Escherichia coli are bacterial species most frequently encountered in human infections, and many serious illnesses can be observed in the hospital environment...

Fast, sensitive and cheap determination of pathogenic bacteria is extremely important in many branches, for example biotechnology, quality control, analysis of samples and antimicrobial therapy. The development and application of analytical techniques in practice could provide new possibilities in this regard. The bacterial pathogen Staphylococcus...

Microorganisms like bacteria, viruses, fungi, etc. have a major impact on human, animal, and plant life. Several decades ago it was observed that intact cells suspended in an isotonic salt solution moved toward the anode and the mobility was proportional to the density of the charge located on the cell surfaces. Since then, efforts have been made t...

The early cancer diagnosis increases the possibility of total recovery. The infection of Helicobacter pylori is associated with gastric cancer, the second most common cancer in the world. The determination of volatile organic compounds (VOCs) excreted by stomach tissue and bacteria culture has been investigated. Solid-phase microextraction (SPME) w...

Hyperlink robust biocompatible solid-phase microextraction (SPME) devices were prepared using continuous bed (monolithic) restricted-access media (RAM) as the SPME capillary insert. The RAM-based SPME approach was able to simultaneously separate proteins from a biological sample, while directly extracting the active components of caffeine, paraceta...

The importance of continuous beds (monoliths) as separation materials is connected with their better chromatographic properties and easier preparation in comparison to particulate-packed columns. Moreover the tuning of porosity as well as surface chemistry can lead to obtaining of highly selective materials, especially useful in separation of biolo...

Helicobacter pylori can cause gastritis and peptic ulcers and is directly associated with the development of gastric cancer. There are many types of diagnostic methods used to identification H. pylori (invasive and non-invasive), but these methods usually require time-consuming and laborious procedures and therefore are not capable of fast diagnosi...

Several extraction techniques, such as Soxhlet extraction, solid phase extraction using molecularly imprinted polymer, matrix solid phase dispersion, and supercritical fluid extraction were evaluated for the isolation and purification of phenolic compounds, e.g., benzoic acids from natural samples of Melissa officinalis. The extracts of benzoic aci...

The differences in surface charge of different bacteria can be exploited for their separation by capillary electrophoresis. However, this method of separation of microorganisms is beset with various drawbacks such as adhesion of bacteria to the fused silica surface or cluster formation. To overcome these phenomena we investigated the addition of po...

The differences in surface charge of different bacteria can be exploited for their separation by capillary electrophoresis. However, this method of separation of microorganisms is beset with various drawbacks such as adhesion of bacteria to the fused silica surface or cluster formation. To overcome these phenomena we investigated the addition of po...

Monolithic supports are a new generation of stationary phases which are typically prepared using a simple molding process carried out within the confines of the capillary. They provide high rates of mass transfer at lower pressure drops, enable much faster separations and the nature of the pores allows easy permeability for large molecules. This re...

This article looks at the use of electromigration techniques to determine microorganisms, such as viruses, bacteria and other biologically important macromolecules (erythrocytes), in medical analyses. It was found that electromigration techniques could be used for the identification of several viruses, including the identification of a specific mar...

Like other colloidal particles bacteria have a surface charge that originates from the ionization of surface molecules and of the adsorption of ions from solution. Bacterial cell wall and membranes containing numerous proteins, lipid molecules, teichoic acids, lipopolisaccharides which give them characteristic charge. Therefore, bacterial cells und...

Differences in the surface charges of bacteria can be exploited for their separation by capillary electrophoresis. Because of their low electrophoretic mobility, the separation is not always easy to perform, especially in the presence of the electroosmotic flow. Elimination of electroosmotic flow by capillary wall modification with γ-(trimethoxysil...