Evgeniya S Seryogina

Orel State University named after I.S.Turgenev · Anatomy, physiology, human hygiene and ecology

Animal models mimicking human transient ischemic attack (TIA) and cerebral microinfarcts are essential tools for studying their pathogenetic mechanisms and finding methods of their treatment. Despite its advantages, the model of single arteriole photothrombosis requires complex experimental equipment and highly invasive surgery, which may affect th...

The transmembrane receptor for advanced glycation end products (RAGE) is a signaling receptor for many damage‐ and pathogen‐associated molecules. Activation of RAGE is associated with inflammation and an increase in reactive oxygen species (ROS) production. Although several sources of ROS have been previously suggested, how RAGE induces ROS product...

This work shows the application of LSCI for mapping the cerebral vessels of a laboratory animal, and also presents the time-frequency processing of the registered signal. Thus, we expand the capabilities of the existing LSCI approach and demonstrate spatial mapping of blood flow fluctuations.

Improving noninvasive approaches to monitoring blood microcirculation is an urgent topic in modern biomedical engineering. One of the actively developing methods is laser speckle contrast imaging (LSCI), which allows not only the visualization of microvessels but also the quantitative measurements of microcirculatory blood flow. This work shows the...

Alterations in function of hypoxanthine guanine phosphoribosyl transferase (HPRT), one of the major enzymes involved in purine nucleotide exchange, lead to overproduction of uric acid and produce various symptoms of Lesch-Nyhan syndrome (LNS). One of the hallmarks of LNS is maximal expression of HPRT in the central nervous system with the highest a...

In this work, we demonstrate the results of measuring the optical properties of rat liver tissue slices after the liver itself underwent the perfusion procedure with an isotonic solution. The approach is suggested as a means to attenuate the influence of blood absorption on recorded characteristics and demonstrates its effectiveness in changing the...

The article is devoted to the analysis of skin perfusion oscillation processes with wild-type and synuclein-deficient mice with three knockout (KO) types: αγ-KO, β-KO, and αβγ-KO. The role of synucleins in tissues that exclude the central nervous system remains sparsely studied. Laser Doppler flowmetry was used to investigate the potential involvem...

Despite the fact that both acute cardiac cessation and respiratory arrest are the reasons of brain ischemia, its hemodynamics, oxygenation and structural pathology differs. This article represents blood re-distribution and neuronal tissue degeneration during the first decade of minutes after the breathing and heart beating violation. The results sh...

Introduction : PolG-alpha is a nuclear-encoded enzyme which provides replication and repair of mitochondrial DNA. D257A mutation of PolG-alpha leads to change in the N-terminal ”proofreading” domain, which deprives the enzyme of 3′-5′ exonuclease activity, resulting in accumulation of mutations in the mitochondrial genome. Materials and methods : M...

The impairments of cerebral blood flow microcirculation brought on by cardiac and respiratory arrest were assessed with multi-modal diagnostic facilities, utilising laser speckle contrast imaging, fluorescence spectroscopy and diffuse reflectance spectroscopy. The results of laser speckle contrast imaging show a notable reduction of cerebral blood...

A device for optical biopsy with a fluorescence spectroscopy channel and a fine-needle optical probe for use in fine-needle aspiration biopsy of liver tumors is described. To test the developed device, experimental measurements of the fluorescence of internal organs of a laboratory rat were carried out in vivo while exposing the tissue surface to m...

Anthropogenic activity causes the introduction of zinc compounds into the biological cycle in mining and processing sites and its accumulation in organs and tissues, causing systemic toxicity. A cumulative effect of zinc is predominantly neurotoxic and it also affects the respiratory, cardiovascular and digestive systems. This study evaluates the e...

To determine optimal lighting conditions for contrast imaging of surgical objects, optical characteristics of biological tissues and spectral characteristics of smart light "LED light" source based on RGBW LED are compared. The spectral characteristics of tissues and organs have been investigated. Optimal lighting conditions for contrast imaging of...

The paper describes the results of experimental studies using custom developed optical biopsy system for diagnostics in vivo during the procedure of fine needle aspiration biopsy. Experimental studies were conducted in laboratory mice with inoculated hepatocellular carcinoma. The measurements were carried out using fluorescence spectroscopy and dif...

The aim of the study was to compare the metabolic activity of brain cortex after the acute hypoxia caused by the impairment of breathing or blood circulation. Male Wistar rats were randomized in two groups: impaired breathing and blood circulation failure groups. Fluorescence under 365 and 450 nm excitation and diffuse reflectance intensity at 550-...

Laser speckle contrast imaging of the microcirculatory bed of the pancreas is performed, which allows its condition to be assessed and thereby is an additional valuable tool for making a diagnostic decision and dynamically monitoring the effectiveness of the treatment for pathology of the abdominal organs. Laparoscopic operations on the pancreas ar...

During surgery, it is important for the surgeon to determine the optimal lighting conditions for contrast imaging of the operated objects. The paper analyzes various parameters of a controlled colour-dynamic surgical lamp based on a powerful led when contrasting individual organs and tissues of model animals, as well as optical parameters of the or...

The paper describes the methodology and technical implementation of a multimodal approach for optical diagnostics in hepatopancreatobiliary organs focal and diffuse neoplasms. Fine needle aspiration biopsy technique and following cytological examination show its effectiveness and safety but its performing takes several days. However, the problem of...

Background. Anthropogenic human activity contributes to the inclusion of zinc compounds in the biological cycle in areas where there is pollution with this metal. This leads to the accumulation of the substance in organs and tissues, which can have a neurotoxic effect on the body. Aim. To evaluate the toxic effect of zinc succinate on the structure a...

Diagnosis and treatment of acute destructive pancreatitis is one of the most urgent problems of abdominal surgery. To obtain additional diagnostic information about the microcirculation state and metabolic processes, various imaging techniques are widely used during surgical interventions. One of the most perspective techniques for in vivo microcir...

The paper describes the experiment at pancreas ischemia model carried out by combining the methods of fluorescence spectroscopy and laser speckle contrast imaging in laboratory mice. The measurement was performed before and after ischemia to evaluate the relationship between the changes of blood flow and metabolic activity. The results showed high...

Fine needle aspiration biopsy technique and following histological examination show its effectiveness and safety but its performing takes several time. However, the problem of real-time analysis of pathological changes in tissues remains relevant. We demonstrate optical fine-needle biopsy method, combining a fine needle (17.5G) and a fiber-optic pr...

Determination of optimal lighting conditions for contrast imaging of operated objects is important for the surgeon. In this paper, we consider various parameters of a controlled color-dynamic surgical light based on a powerful LED when contrasting individual organs and tissues of model animals. The obtained results will allow to develop a library o...

Modern fluorescent modalities play an important role in the functional diagnostic of various physiological processes in living tissues. Utilizing the fluorescence spectroscopy approach we observe the circulation of fluorescent-labelled nanocapsules with rhodamine tetramethylrhodamine in a microcirculatory blood system. The measurements were conduct...

This studiy was carried out on groups of clinically healthy male Wistar rats. Animals received distilled drinking water ad libitum for 1 month, water containing succinic acid, water containing zinc sulphate and succinate zinc. Using the method of fluorescence spectroscopy, the parameters of brain metabolism in vivo in a model of laboratory rats was...

Using fluorescence spectroscopy system with the fibre-optical probe, we investigated the dynamics of propagation and circulation in the microcirculatory system of experimental nanocapsules fluorescent-labelled (rhodamine TRITC) nanocapsules. The studies were carried out in clinically healthy Wistar rats. The model animals were divided into control...

Presently non-invasive methods of optical diagnostics based on spectrophotometry and fluorescence analysis find a wide application in biomedicine, pharmacology, chemistry, etc. [1]. Fluorescence spectroscopy has a high potential in the field of drug research not only for drug development, but also during preclinical and clinical trials [2]. An impo...

This article presents the results of the study of the pressure applied on optical diagnostic probes as a significant factor affecting the results of measurements. During stepwise increasing and decreasing of local pressure on skin we conducted measurements using the methods of laser Doppler flowmetry and fluorescence spectroscopy. It was found out...