Evelin DombayUniversity of St Andrews · School of Medicine
Evelin Dombay
Doctor of Philosophy
About
6
Publications
2,055
Reads
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8
Citations
Citations since 2016
Introduction
I am a former Ph.D. researcher from the University of St Andrews with a great interest in bacterial dormancy, TB diagnostics, and treatment monitoring.
Publications
Publications (6)
Tuberculosis is one of the most serious infectious diseases, and its treatment is highly dependent on early detection. Microscopy-based analysis of sputum images for bacilli identification is a common technique used for both diagnosis and treatment monitoring. However, it a challenging process since sputum analysis requires time and highly trained...
Tuberculosis is one of the most serious infectious diseases, and its treatment is highly dependent on early detection. Microscopy-based analysis of sputum images for bacilli identification is a common technique used for both diagnosis and treatment monitoring. However, it a challenging process since sputum analysis requires time and highly trained...
Relapse in Tuberculosis (TB) patients represents an important challenge to improve treatment. A large number of patients undergo relapse even after what was thought to be a successful treatment. Lipid rich (LR) bacteria, surviving treatment, are thought to play a key role in patient relapse. The presence of bacteria with intracellular lipid bodies...
Relapse in Tuberculosis (TB) patients represents an important challenge to improve treatment. A large number of patients undergo relapse even after what was thought to be a successful treatment. Lipid rich (LR) bacteria, surviving treatment, are thought to play a key role in patient relapse. The presence of bacteria with intracellular lipid bodies...
Questions
Question (1)
Hi there,
I have designed a set of primers and performed RT-qPCR melt curve analysis to check for potential primer-dimer formation.
I keep on getting very odd looking melting curves. My NTC and neat sample does not seem to give an extra peak but when I dilute my sample down to 1/100 another peak appears at an earlier temperature than my template (circled with red).
Also, when I only look at my undiluted sample there is another wide peak at the very beginning, that I am not sure about what it is (second image).
Has anyone ever experienced something like this? Could it mean primer-dimers?
