Evelin DombayUniversity of St Andrews · School of Medicine
Evelin Dombay
Doctor of Philosophy
About
8
Publications
2,142
Reads
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10
Citations
Citations since 2017
Introduction
I am a former Ph.D. researcher from the University of St Andrews with a great interest in bacterial dormancy, TB diagnostics, and treatment monitoring.
Publications
Publications (8)
Microscopy analysis of sputum images for bacilli screening is a common method used for both diagnosis and therapy monitoring of tuberculosis (TB). Nonetheless, it is a challenging procedure, since sputum examination is time-consuming and needs highly competent personnel to provide accurate results which are important for clinical decision-making. I...
Tuberculosis is one of the most serious infectious diseases, and its treatment is highly dependent on early detection. Microscopy-based analysis of sputum images for bacilli identification is a common technique used for both diagnosis and treatment monitoring. However, it a challenging process since sputum analysis requires time and highly trained...
Tuberculosis is one of the most serious infectious diseases, and its treatment is highly dependent on early detection. Microscopy-based analysis of sputum images for bacilli identification is a common technique used for both diagnosis and treatment monitoring. However, it a challenging process since sputum analysis requires time and highly trained...
Relapse in Tuberculosis (TB) patients represents an important challenge to improve treatment. A large number of patients undergo relapse even after what was thought to be a successful treatment. Lipid rich (LR) bacteria, surviving treatment, are thought to play a key role in patient relapse. The presence of bacteria with intracellular lipid bodies...
Relapse in Tuberculosis (TB) patients represents an important challenge to improve treatment. A large number of patients undergo relapse even after what was thought to be a successful treatment. Lipid rich (LR) bacteria, surviving treatment, are thought to play a key role in patient relapse. The presence of bacteria with intracellular lipid bodies...
Questions
Question (1)
Hi there,
I have designed a set of primers and performed RT-qPCR melt curve analysis to check for potential primer-dimer formation.
I keep on getting very odd looking melting curves. My NTC and neat sample does not seem to give an extra peak but when I dilute my sample down to 1/100 another peak appears at an earlier temperature than my template (circled with red).
Also, when I only look at my undiluted sample there is another wide peak at the very beginning, that I am not sure about what it is (second image).
Has anyone ever experienced something like this? Could it mean primer-dimers?
