Evann E. Hilt

Evann E. Hilt
University of Minnesota Medical Center, Fairview

Ph.D. D(ABMM)

About

59
Publications
5,905
Reads
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1,831
Citations
Introduction
Additional affiliations
August 2014 - December 2019
Loyola University Chicago
Position
  • PhD Student
August 2012 - June 2014
Loyola University Chicago
Position
  • Master's Student
May 2011 - August 2011
Baylor College of Medicine
Position
  • Summer Medical and Research Training (SMART) Program Student
Education
August 2012 - August 2014
Loyola University Medical Center
Field of study
  • Infectious Disease and Immunology
August 2008 - May 2012
Augustana College
Field of study
  • Pre-Medicine and Biology

Publications

Publications (59)
Article
Full-text available
Background The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused global disruption of human health and activity. Being able to trace the early outbreak of SARS-CoV-2 within a locality can inform public health measures and provide insights to contain or prevent viral transmission. Investigation of the transmission history requi...
Article
Objectives This study aimed to assess whether the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Epsilon variant (B.1.429/427) is more virulent, leading to more hospitalization and more severe disease requiring intensive care unit (ICU) admission. Methods SARS-CoV-2 genomic surveillance was performed on respiratory samples from 231 u...
Article
Full-text available
Frequent and widespread testing of members of the population who are asymptomatic for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for the mitigation of the transmission of the virus. Despite the recent increases in testing capacity, tests based on quantitative polymerase chain reaction (qPCR) assays cannot be easily de...
Article
Objectives: To compare urinary tract infection (UTI) symptom resolution rates at 7-10 days in symptomatic women randomized to treatment based on standard urine culture (SUC) versus expanded quantitative urine culture (EQUC) results. Methods: Women ≥18 years old who responded "yes" to "do you feel you have a UTI?" agreed to urethral catheterizati...
Article
Full-text available
Gardnerella is a frequent member of the urogenital microbiota. Given the association between Gardnerella vaginalis and bacterial vaginosis (BV), significant efforts have been focused on characterizing this species in the vaginal microbiota. However, Gardnerella also is a frequent member of the urinary microbiota. In an effort to characterize the ba...
Article
Full-text available
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused one of the worst pandemics in recent history. Few reports have revealed that SARS-CoV-2 was spreading in the United States as early as the end of January. In this study, we aimed to determine if SARS-CoV-2 had been circulating in the Los Angeles (LA) area at a time...
Preprint
Full-text available
Background The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global disruption to human health and activity. Being able to trace the early outbreak of SARS-CoV-2 within a locality will inform public health measures and provide insights to contain or prevent the viral transmission to save lives. Investigation of the trans...
Article
Candida auris is an emerging multidrug-resistant yeast. We describe an ongoing C. auris outbreak that began in October 2019 in Los Angeles, California, USA. We used genomic analysis to determine that isolates from 5 of 6 patients belonged to clade III; 4 isolates were closely related.
Article
Full-text available
Aeromonas hydrophila resides in a variety of aquatic environments. Infections with A. hydrophila mainly occur after contact with fresh or brackish water. Nosocomial infections with A. hydrophila can also occur. A. hydrophila infections can be difficult to treat due to both intrinsic and acquired antimicrobial resistance (AMR) mechanisms. In 2018-19...
Article
Background: Candida auris is an emerging multidrug-resistant pathogen associated with outbreaks in hospitals and skilled nursing facilities (SNFs). Patients with C. auris can have invasive disease or asymptomatic colonization. Because C. auris can be difficult to treat and eradicate in the environment, the CDC recommends using contact precautions a...
Preprint
Full-text available
Los Angeles (LA) County has sustained a large outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To learn about the transmission history of SARS-CoV-2 in LA County, we sequenced 142 viral genomes from unique patients seeking care at UCLA Health System. 86 of these genomes are from samples collected before April 19, 2020. We f...
Article
Full-text available
A novel coronavirus known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the ongoing Coronavirus Disease 2019 (COVID-19) pandemic. In this study, we performed a comprehensive epidemiological and genomic analysis of SARS-CoV-2 genomes from 10 patients in Shaoxing (Zhejiang Province), a mid-sized city outside of the e...
Preprint
Full-text available
Conventional methods for viral genome sequencing largely use metatranscriptomic approaches or, alternatively, enrich for viral genomes by amplicon sequencing with virus-specific PCR or hybridization-based capture. These existing methods are costly, require extensive sample handling time, and have limited throughput. Here, we describe V-seq, an inex...
Preprint
Full-text available
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is due to the high rates of transmission by individuals who are asymptomatic at the time of transmission. Frequent, widespread testing of the asymptomatic population for SARS-CoV-2 is essential to suppress viral transmission and is a key element in safely reopening soc...
Article
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is due to the high rates of transmission by individuals who are asymptomatic at the time of transmission. Frequent, widespread testing of the asymptomatic population for SARS-CoV-2 is essential to suppress viral transmission and is a key element in safely reopening soc...
Article
Introduction Previous work has shown that the vaginal microbiome decreases in Lactobacillus predominance and becomes more diverse following menopause. It has also been shown that estrogen therapy restores Lactobacillus-dominance in the vagina, and that topical estrogen is associated with OAB symptom improvement. We now know that the bladder contain...
Article
Invasive fungal infections are increasing in prevalence due to an expanding population of immunocompromised individuals. To reduce morbidity and mortality, it is critical to accurately identify fungal pathogens to guide treatment. Current methodologies rely on histopathology, fungal culture, and serology, which are often insufficient for diagnosis....
Article
Full-text available
BACKGROUND Since the discovery of the bladder microbiome (urobiome), interest has grown in learning whether urobiome characteristics have a role in clinical phenotyping and/or provide opportunities for novel therapeutic approaches for women with common forms of urinary incontinence (UI). OBJECTIVES To test our hypothesis that the bladder urobiome...
Preprint
A novel coronavirus known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the ongoing COVID-19 pandemic. In this study, we performed a comprehensive epidemiological and genomic analysis of SARS-CoV-2 genomes from ten patients in Shaoxing, a mid-sized city outside of the epicenter Hubei province, China, during the ear...
Article
Aerococcus urinae is increasingly recognized as a potentially significant urinary tract bacterium. A. urinae has been isolated from urine collected from both males and females with a wide range of clinical conditions, including urinary tract infection (UTI), urgency urinary incontinence (UUI) and overactive bladder (OAB). A. urinae is of particular...
Article
Full-text available
Objective: To characterize the bladder microbiota of continent adult women. Design: Cross-sectional study of adult women who contributed catheterized urine samples, completed validated symptom questionnaires and provided demographic data. Setting: US academic medical center. Population: Well-characterized continent adult women. Methods: Pa...
Article
Full-text available
Introduction and hypothesis The current etiology of interstitial cystitis/painful bladder syndrome (IC/PBS) is poorly understood and multifactorial. Recent studies suggest the female urinary microbiota (FUM) contribute to IC/PBS symptoms. This study was designed to determine if the FUM, analyzed using mid-stream voided urine samples, differs betwee...
Preprint
OBJECTIVE: Current etiology of interstitial cystitis/painful bladder syndrome (IC/PBS) is poorly understood and multifactorial. Recent studies suggest the female urinary microbiota (FUM) contribute to IC/PBS symptoms. This study was designed to determine if the FUM, analyzed using mid-stream voided urine samples, differs between IC/PBS patients and...
Article
Full-text available
Metagenomic analyses have indicated that the female bladder harbors an indigenous microbiota. However, there are few cultured reference strains with sequenced genomes available for functional and experimental analyses. Here we isolate and genome-sequence 149 bacterial strains from catheterized urine of 77 women. This culture collection spans 78 spe...
Article
Full-text available
Urinary tract infection (UTI) is clinically important, given that it is one of the most common bacterial infections in adult women. However, the current understanding of UTI remains based on a now disproven concept that the urinary bladder is sterile. Thus, current standards for UTI diagnosis have significant limitations that may reduce the opportu...
Article
Full-text available
Objective: To assess urinary symptoms associated with urinary tract infection (UTI) in a urogynecologic population of women. Methods: In this cohort study, we enrolled 150 urogynecologic patients who completed the validated UTI Symptom Assessment questionnaire and contributed transurethral catheterized urine samples. The primary measure (UTI dia...
Article
Full-text available
Objective The objective of this study was to characterize the bladder microbiota in pregnancy. Methods A prospective observational study of 51 pregnant women, admitted to a tertiary care hospital, who underwent straight catheterization urine collection or transurethral Foley catheter placement. 16S rRNA gene sequencing and enhanced quantitative uri...
Article
Full-text available
A draft genome sequence for a urinary isolate of Nosocomiicoccus ampullae (UMB0853) was investigated. The size of the genome was 1,578,043 bp, with an observed G+C content of 36.1%. Annotation revealed 10 rRNA sequences, 40 tRNA genes, and 1,532 protein-coding sequences. Genome coverage was 727× and consisted of 32 contigs, with an N50 of 109,831 b...
Article
Full-text available
While Lactobacillus crispatus contributes to the stability of normal vaginal microbiota, its role in urinary health remains unclear. As part of an on-going attempt to characterize the female urinary microbiota, we report the genome sequence of an L. crispatus strain isolated from a woman displaying no lower urinary tract symptoms.
Article
Full-text available
Bacterial surveys of the vaginal and bladder human microbiota have revealed an abundance of many similar bacterial taxa. As the bladder was once thought to be sterile, the complex interactions between microbes within the bladder have yet to be characterized. To initiate this process, we have begun sequencing isolates, including the clinically relev...
Data
Presence/absence of gene functionality within the four bladder isolates. (XLSX)
Data
FASTA sequences for the individual prophage gene clusters. (ZIP)
Data
Phylogenetic tree based on 16S rRNA gene. Maximum-Likelihood phylogenetic tree for the 16S rRNA gene. Strains isolated from the bladder are indicated in red. (TIF)
Data
Presence/absence of virulence genes within the four bladder isolates. (XLSX)
Data
Prophage sequences, clusters, and putative origins within the 39 Gardnerella genomes. (XLSX)
Data
PCR primers for amplification of Gv-specific carbohydrate metabolism coding regions. (XLSX)
Data
Phylogenetic analysis of VLY gene. (A) Amino acid sequence alignment of four bladder Gardnerella isolates and representatives from other clades; clades are indicated to the left of each strain/isolate name. Mismatches within the alignment are highlighted (red/blue text). (B) Maximum-Likelihood phylogenetic tree for the VLY gene. Branch supports are...
Data
List of 35 genomes retrieved from NCBI for genome comparisons. (XLSX)
Data
Core genes identified for the 39 Gardnerella genomes examined. (XLSX)
Article
Full-text available
The genus Escherichia includes pathogens and commensals. Bladder infections (cystitis) result most often from colonization of the bladder by uropathogenic E. coli strains. In contrast, a poorly defined condition called asymptomatic bacteriuria results from colonization of the bladder with E. coli strains without symptoms. As part of an on-going att...
Article
Full-text available
Enhanced Quantitative Urine Culture (EQUC) detects live microorganisms in the vast majority of urine specimens reported as “no growth” by the Standard Urine Culture protocol. Here, we evaluated an expanded set of EQUC conditions (Expanded Spectrum EQUC) to identify an optimal version that provides a more complete description of uropathogens in wome...
Article
Full-text available
Background: The role of calcium oxalate crystals and deposits in UTI pathogenesis has not been established. The objectives of this study were to identify bacteria present in pediatric urolithiasis and, using in vitro and in vivo models, to determine the relevance of calcium oxalate deposits during experimental pyelonephritis. Methods: Pediatric...
Article
Full-text available
Introduction and hypothesis Many adult women have resident urinary bacteria (urinary microbiome/microbiota). In adult women affected by urinary urgency incontinence (UUI), the etiologic and/or therapeutic role of the urinary microbiome/microbiota remains unknown. We hypothesized that microbiome/microbiota characteristics would relate to clinically...
Article
Full-text available
Bacterial DNA and live bacteria have been detected in human urine in the absence of clinical infection, challenging the prevailing dogma that urine is normally sterile. Urgency urinary incontinence (UUI) is a poorly understood urinary condition characterized by symptoms that overlap urinary infection, including urinary urgency and increased frequen...
Article
Full-text available
Our previous study showed that bacterial genomes can be identified using 16S rRNA sequencing in urine specimens of both symptomatic and asymptomatic patients who are culture negative using standard urine culture protocols. In the present study, we used a modified culture protocol that included plating larger volumes of urine, incubation under varie...