Eva E RufinoUniversity of Granada | UGR · Department of Biochemistry and Molecular Biology
Eva E Rufino
Doctor of Biology
teaching and research staff
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64
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Introduction
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October 2012 - present
January 2007 - present
April 2005 - September 2011
Publications
Publications (64)
There is currently a worldwide consensus and recognition of the undoubted health benefits of the so-called Mediterranean diet, with its intake being associated with a lower risk of mortality. The most important characteristics of this type of diet are based on the consumption of significant amounts of fruit, vegetables, legumes, and nuts, which pro...
Natural products are bioactive compounds synthesized by terrestrial and marine plants, microorganisms and animals. Traditionally, they have been used in the prevention and treatment of various human diseases in different cultures. In parallel, chemical derivatives of these natural compounds have been used in order to enhance their bioactivities. Du...
Natural products are bioactive compounds synthesized by terrestrial and marine plants, microorganisms and animals. Traditionally, they have been used in the prevention and treatment of various human diseases in different cultures. In parallel, chemical derivatives of these natural compounds have been used in order to enhance their bioactivities. Du...
ANTIPROLIFERATIVE ACTIVITY OF ERYTHRODIOL ON HEP-G2 CELLS. ROLE OF THE ANTIOXIDANT RESPONSE AND CHANGES IN THE PROTEOME
Erythrodiol (EO) is a pentacyclic triterpenic alcohol found in olive tree leaves and olive oil, and it has important effects on the health properties and quality of olive oil. In this study, we characterized the cytotoxic effects of EO on human hepatocarcinoma (HepG2) cells by studying changes in cell viability, reactive oxygen species (ROS) produc...
Many natural products are bioactive compounds synthesized by terrestrial and marine plants, microorganisms and animals, whose main objective is to prevent them from attacks by predators and/or pathogens. Traditionally, different cultures have been using, since ancient times, these compounds for the prevention and treatment of various human diseases...
Preface to “Anticancer Properties of Natural and Derivative Products”
A Thematic Book Dedicated in Memoriam to Professor Eduardo García Peregrín, a Pharmaceutical Biochemist with the Heart and the Soul of a Biochemical Biologist.
Many natural products are bioactive compounds synthesized by terrestrial and marine plants, microorganisms and animals,...
In the chronology of Biochemistry, as a new science that emerged in the mid-nineteenth century after its separation from Organic Chemistry and Physiology, its beginnings were characterized by an intense search and subsequent isolation and characterization of different organic compounds that were part of the chemical composition of living organisms...
Our cells and organs are threatened and, in most cases, constantly subjected to the aggression of numerous situations, both endogenous, characterized by unfavorable genetics, and exogenous, by deficient or inadequate nutrition, and even by a hostile environment; in most cases, they ultimately cause a cascade of degenerative and cardiovascular disea...
Recent evidence has shown that inflammation can contribute to all tumorigenic states. We have investigated the anti-inflammatory effects of a diamine-PEGylated derivative of oleanolic acid (OADP), in vitro and in vivo with inflammation models. In addition, we have determined the sub-cytotoxic concentrations for anti-inflammatory assays of OADP in R...
Bioactive metabolites isolated from medicinal mushrooms (MM) used as supportive treatment in conventional oncology have recently gained interest. Acting as anticancer agents, they interfere with tumor cells and microenvironment (TME), disturbing cancer development/progression. Nonetheless, their action mechanisms still need to be elucidated. Recent...
Abstract: Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Our recent studies have shown that the diamine-(PEG)ylated oleanolic acid (OADP) has strong anti-tumor effects in HCCs. In this study, we evaluated the anti-tumor mechanisms of OADP in the HepG2 HCC cell line. The cytotoxicity results showed that HepG2 cell viability...
Natural products have a significant role in the development of new drugs, being relevant the pentacyclic triterpenes extracted from Olea europaea L. Anticancer effect of uvaol, a natural triterpene, has been scarcely studied. The aim of this study was to understand the anticancer mechanism of uvaol in the HepG2 cell line. Cytotoxicity results showe...
Maslinic acid (MA) is a natural triterpene from Olea europaea L. with multiple biological properties. The aim of the present study was to examine MA's effect on cell viability (by the MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key antioxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10) cell...
Natural products have a significant role in the development of new drugs, being relevant the pentacyclic triterpenes extracted from Olea europaea. Anticancer effect of uvaol, a natural triterpene, has been scarcely studied. The aim of this study was to understand the anticancer mechanism of uvaol in HepG2 cell line. Cytotoxicity results showed a se...
Maslinic acid (MA) is a natural triterpene from Olea europaea whose pharmacological functions have been showed. The objective of this study was to examine MA effect on cell viability (by MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key anti-oxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10)...
We have designed and synthesized two novel cobalt coordination compounds using bumetanide (bum) and indomethacin (ind) therapeutic agents. The anti-inflammatory effects of cobalt metal complexes with ind and bum were assayed in lipopolysaccharide stimulated RAW 264.7 macrophages by inhibition of nitric oxide production. Firstly, we determined the c...
Lung cancer is one of the most prevalent types of malignancies worldwide, accounting for 1.6 million deaths every year. Despite last efforts, the overall survival rate at five years after diagnosis is under 15%. Therefore, new approaches are needed to improve the current clinic. Suicide gene therapy is an interesting technology which consists in ex...
The present study examines whether fish density affects behavioural tests, feed demand, and different parameters indicative of the oxidative status of the liver and brain of Orechromis sp. to identify welfare indicators for fish culturing. The fish were maintained at two commercial densities (5.6 and 16.2 kg/m3) and hand fed for 4 weeks (four repli...
Oleanolic acid (1) pentacyclic triterpene present in olive pomace, is known to induce apoptosis and to be anti-tumor, but high concentrations of this product are necessary to produce its cytotoxic effects. The 3-O-succinyl-28-O-benzyl oleanolate derivative (4), present a greater cytotoxicity and apoptosis effects than its natural precursor, oleanol...
Chromatin remodeling complexes are crucial for the viability of the cells due to their role in regulating interactions between DNA and histones and, therefore, modifying the accessibility of the genetic nformation to the transcriptional machinery. This relevance can also been seen in the SWI/SNF complex that has been
associated with cancer in the...
Background:
Metabolic syndrome is a set of pathologies among which stand out the obesity, which is related to the lipid droplet accumulation and changes to cellular morphology regulated by several molecules and transcription factors. Maslinic acid (MA) is a natural product with demonstrated pharmacological functions including anti-inflammation, an...
This study aims to evaluate the effects of dietary arginine (Arg) surplus on gilthead sea bream growth, intestinal nutrient absorption capacity, hepatic and intestinal amino acid (AA) metabolism, and oxidative status. For that purpose a 6 weeks growth trial was performed with gilthead sea bream juveniles fed four isolipidic (18%) and isoproteic (44...
NADPH plays a central role in reductive biosynthesis of membrane lipids, maintenance of cell integrity, protein synthesis and redox balance maintenance. Hence, NADPH is involved in the growth and proliferation processes. In addition, it has been shown that changes in nutritional conditions produced changes in NADPH levels and growth rate. Maslinic...
Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mitochondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dose-...
(A) Left: Western bloting of the levels of Bid (p24) and t-Bid (p15) in Caco-2 cells. Right: Western bloting of the levels of Bid (p24) in HT29 cells. Note that not levels of t-Bid (p15) were detected in this cell line. Cells were treated with maslinic acid (MA) at IC50 and IC80 concentrations for 4h. (B) Left: Western bloting of levels of Bax (p23...
A suplementação de dietas com arginina modula a resposta enzimática antioxidante no fígado e no intestino de juvenis de dourada (Sparus aurata) Abstract The present study evaluated the effect of dietary arginine (0.5, 1 or 2% Arg) supplementation on liver and intestine antioxidant enzymatic responses of gilthead sea bream juveniles. At the end of t...
A suplementação de dietas com arginina acima das necessidades não tem efeito no crescimento, no metabolismo dos aminoácidos e na composição corporal em juvenis de dourada (Sparus aurata) Abstract The present study evaluated the effect of dietary arginine (0.5, 1 or 2% Arg) supplementation on growth performance, feed utilization, body composition an...
Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids presents in olive, have important properties for health and disease prevention. MA selectively inhibits cell proliferation of the HT29 human colon-cancer cell line by inducing selective apoptosis. For measuring the MA and OA concentration inside the cell and in the culture mediu...
La cúrcuma es un producto natural obtenido de la planta Curcuma longa de la que muchos estudios han demostrado sus efectos beneficiosos para la salud ya que presenta propiedades antioxidantes, antiinflamatorias y anticancerígenas, entre otras. En el presente trabajo se evalúa el efecto anticancerígeno de la cúrcuma mediante ensayos in vitro en célu...
El aceite de oliva posee numerosos efectos beneficiosos para la salud ya que en su composición se pueden encontrar diferentes compuestos a los que se les atribuyen propiedades tales como antioxidantes, anticancerígenas, antiinflamatorias, etc. Entre los triterpenos más abundantes en el aceite de oliva se encuentra el ácido maslínico, del cual se ob...
Hydroxytyrosol is the main natural polyphenol present in olive oil, and in the past few
years it has been related to a great number of beneficial properties among which
outstand its antioxidant effect, antiinflammatory capacity and potential as antitumor
agent. In the context of cancer, hydroxytyrosol shows a protective role derived from the
arrest...
Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no...
Pentacyclic triterpenes are natural substances, synthesized and present in variable amounts in a large number of terrestrial and aquatic plants, acting as antioxidants and antimicrobials, and also as poisons, antibiotics, protease inhibitors, and so on. From the organic chemical point of view, they are compounds derived from isoprene. These are com...
SMARCA4 is the catalytic subunit of the SWI/SNF chromatin-remodeling complex, which alters the interactions between DNA and histones
and modifies the availability of the DNA for transcription. The latest deep sequencing of tumor genomes has reinforced the
important and ubiquitous tumor suppressor role of the SWI/SNF complex in cancer. However, alth...
MicroRNAs (miRNAs) are a recently discovered class of small RNA molecules that regulate gene expression at the post-transcriptional level.MiRNAs maintain the appropriate balance important process in tumor development including proliferation, differentiation,apoptosis, senescence, angiogenesis and metastasis. MiRNA deregulationmayhave profoundconseq...
We report that a novel triterpenoid, (2a,3b)-2,3-dihydroxyolean-12-en-28-oic acid (maslinic acid), isolated from olive pomace from Olea europaea, triggers primarily the extrinsic and later the intrinsic apoptotic pathways in Caco-2 human colon-cancer cells. Apoptosis induced by maslinic acid was confirmed by FACS analysis using annexine-V FICT stai...
NADPH produced in the pentose phosphate pathway (PPP) plays a central role in the reductive biosynthesis of membrane lipids, as well as in the maintenance of cell integrity; also, it has a key part in the synthesis of protein, the other member element. For that NADPH is involved in the growth processes. Our main objective is to study the effects of...
Maslinic acid (MA) is a natural pentacyclic triterpene used as a feed additive to stimulate growth, protein turnover rates, and hyperplasia in fish. To further our understanding of the effects of ration restriction on the cellular mechanisms underlying the action of MA we have used a proteomic procedure based on 2-DE coupled with MS to identify pro...
Maslinic acid (MA) is a pentacyclic triterpene used, at
experimental level, as an additive to stimulate growth, protein-
turnover rates and hyperplasia in fish. In this experiment
we have studied the effects of feeding with MA at
daily fixed ration of 10 g kg
�1 of body mass, on growth,
protein-turnover rates and nucleic acid concentration in
white...
Maslinic acid (MA) is a natural triterpene that can be used as an additive in the diet of trout. We investigated the effects of feeding with MA and a fixed ration (10 g kg)1 body weight) with respect to growth, protein-turnover rates and nucleicacid concentration in the liver of gilthead sea bream grown under fish-farm conditions. Five groups of 12...
Maslinic acid (MA) is a pentacyclic triterpene used as a feed additive to stimulate growth, protein-turnover rates, and hyperplasia in fish. To further our understanding of cellular mechanisms underlying the action of MA, we have used 2-DE coupled with MS to identify proteins differentially expressed in the livers of juvenile gilthead sea bream (Sp...
Maslinic acid, a pentacyclic triterpene found in the protective wax-like coating of the leaves and fruit of Olea europaea L., is a promising agent for the prevention of colon cancer. We have shown elsewhere that maslinic acid inhibits cell proliferation to a significant extent and activates mitochondrial apoptosis in colon cancer cells. In our late...
We have investigated the mechanisms of maslinic acid with regard to its inhibitory effects on the growth of HT29 colon-cancer cells. High concentrations of maslinic acid are present in the protective wax-like coating of olives. Our results show that treatment with maslinic acid results in a significant inhibition of cell proliferation in a dose-dep...
Tesis Univ. Granada. Departamento de Bioquímica y Biología Molecular I. Leída el 24 de julio de 2009
Projects
Project (1)
Terpenes are a large set of compounds, which have taken an important relevance in the scientific literature in recent years. They have a wide variety of biological activities, among which are analgesic effects, hepatoprotective, anti-tumor, anti-inflammatory, anti-oxidants and effectors of the immune system. Maslinic acid (2-α 3 β-dihidroxiolean-12-en-28-oic-acid) is a pentacyclic triterpene 472.7 g / mol, belonging to the group of triterpenoids oleanano. This compound has shown preliminarily similar and even more pronounced effects that structural analogues, including bioactivities as anticarcinogenic, antiinflammatory, antioxidant, activator of the immune system, antiviral, etc. are In terms of structure, a feature common to all triterpene acids, in turn, related to their biological activities, is the importance of the carboxylic group in the C-28 position and the substituent in this case, the hydroxyl group at the C-3 position. (Serra et al., 1994). The presence of an additional hydroxyl group at C-2 of maslinic acid could confer this compound more potent than those of oleanolic acid properties (Marquez-Martin et al., 2006a), as well as the rest of pentacyclic triterpene derivatives. Maslinic acid derivatives should have the same biological properties or even more enhanced as synthesized according to their radicals. That is why, so the phenylethylamine derivative maslinic acid could present some of these capabilities. The benefit of these compounds is such that it may be related to diseases associated with metabolic syndrome. Metabolic syndrome is related to the ability to produce inflammation, proliferation, rupture of apoptosis, besides changes in hormone levels, metabolites, enzyme systems, ion concentration ... all associated with pathologies as known as hypertension, diabetes, obesity , dyslipidemia ... This set of conditions has been extensively studied and is quite known on the subject, but even today continues to prevail in the world population, whether women, men, children, elderly ... etc. Metabolic syndrome is associated with diseases such as hyperinsulinemia, glucose intolerance and type 2 diabetes, hypertension, obesity, dyslipidemia, polycystic ovary syndrome, nonalcoholic fatty liver disease, cancer, vascular risk ...