Eva Ramon-Gallegos

• PhD
• Senior Researcher at National Polytechnic Institute

Ebola virus (EBOV) is a filovirus that causes severe hemorrhagic fever and has a fatality rate between 50 and 90%. The vaccines were developed against the Ebola Zaire species; therefore, it is necessary to develop vaccines against other species to control future outbreaks. The objective of this work was to obtain vaccine candidate peptides against...

The COVID-19 pandemic evidenced the urgent need for rapid, accurate, and scalable diagnostic methods for emerging infectious diseases. Droplet digital reverse transcription loop-mediated isothermal amplification (ddRT-LAMP) is a promising technique for pathogen detection and DNA or RNA quantification. Compared to ddPCR, it simplifies device design,...

SARS-CoV-2 has spread throughout the world since 2019, changing in its genome and leading to the appearance of new variants. This gave it different evolutionary advantages, such as greater infectivity and/or a greater ability to avoid the immune response, which could lead to an increased severity of COVID-19 cases. There is no consistent informatio...

Mycoplasma contamination in cell culture affects the properties of cell lines. Gold standard detection by microbiological culture takes days and requires specialists. The polymerase chain reaction and loop-mediated isothermal amplification (LAMP) are fast molecular options, but LAMP only requires one heating block for DNA amplification. This study...

Nitric oxide (NO) participates in processes such as endothelium-dependent vasodilation and neurotransmission/neuromodulation. The role of NO in epilepsy is controversial, attributing it to anticonvulsant but also proconvulsant properties. Clarification of this dual effect of NO might lead to the development of new antiepileptic drugs. Previous resu...

Cervical carcinoma is the second cause of cancer death in Mexican women. It starts with premalignant lesions known as Intraepithelial Cervical Neoplasia (CIN) that can develop due to infection by Human Papillomavirus (HPV) and other microorganisms. Current CIN therapy involves invasive methods that affect cervix integrity and fertility; we propose...

The WHO has approved the use of several vaccines during the COVID-19 pandemic; experience over the last 2 years has indicated that dose demand can only be covered using more than one design. Therefore, having scientific evidence of the performance of the different vaccines applied in a country is highly relevant. In Mexico, 5 vaccines against sever...

Background Photodynamic therapy (PDT) is used to treat tumors through selective cytotoxic effects. PDT induces damage-associated molecular patterns (DAMPs) expression, which can cause an immunogenic death cell (IDC). In this study we identified potential immunogenic epitopes generated by PDT on triple-negative breast cancer cell line (MDA-MB-231)....

Analyzing the electrical double layer (EDL) in electrical impedance spectroscopy (EIS) measurement at low frequencies remains a challenging task for sensing purposes. In this work, we propose two approaches to deal with the EDL in measuring impedance for particles and non-adherent cells in an electrolytic suspension. The first approach is a simple...

Photodynamic Therapy (PDT) is a non-invasive treatment that has shown its usefulness in eliminating low grade squamous intraepithelial lesion (LSIL) and Human Papilloma Virus (HPV) infection. The response to PDT is variable, how patient´s immunology works during human PDT is unclear and therefore, the main of this work was to characterize the local...

The anti-inflammatory effects of Aloe vera (AV), polysaccharide extract from AV, and extracts from the digestion and colonic fermentation of AV were evaluated using an immortal astrocyte cell line (U373 MG) that develops a neuro-inflammatory profile. Cell viability and inflammatory markers were assessed after stimulation with neuropeptide substance...

Graviola, soursop, or guanabana (Annona muricata L.), is an ethnomedical fruit consumed to alleviate headache, diarrhea, diabetes, and cancer. Pericarp is the inedible part of graviola least studied in comparison to seeds and leaves, even thought, it contains the highest concentration of graviola total polyphenols. Anticancer effect of graviola per...

One of the main obstacles of Photodynamic Therapy (PDT) to damage and destroy abnormal cells is that most photosensitizers (Ps) have a highly hydrophobic nature with a tendency to aggregate in aqueous solutions and the non-specificity towards target cells. Nanotechnology proposes new tactics for the development of monomeric Ps nanotransporters and...

Nanotechnology proposes new applications for the development of nanotransporters and active targeting molecules with the use of biodegradable polymeric nanoparticles to improve the specificity towards target cells. However, these products must comply with safety tests to be endorsed as therapeutic alternatives by regulatory organizations. The goal...

The recently developed One-Step Poly(amidoamine) (OS-PAMAM) dendrimers stand out for their characteristics as the high drug-load capacity and cell-delivery improvement of therapeutic agents. The OS-PAMAM dendrimers have proven to be useful in the biomedical field as nanocarrier or nanosystems for therapy. In the present research it was encouraging...

The main obstacle of Photodynamic Therapy (PDT) to damage and destroy abnormal cells is that most photosensitizers (Ps) have a highly hydrophobic nature with a tendency to aggregate in aqueous solutions and the non-specificity towards target cells. Nanotechnology proposes new tactics for the development of monomeric Ps nanotransporters and active t...

In Mexico, urban rabies has been reduced during the last decade thanks to intensive canine control and vaccination campaigns; however, rabies transmitted by wild animals, especially by bats, has been increasing due to vampire bats feeding on livestock. Vampire bat populations has been controlled by culling with vampiricides, reducing indiscriminate...

Background Breast cancer is the most common malignancy effecting women, and the triple-negative breast cancer (TNBC) subtype is particularly aggressive. This study aimed to evaluate the differential expression pattern of microRNAs (miRNAs) between untreated MDA-MB-231 cells (TNBC cell model) and those that survived photodynamic therapy (PDT) to gai...

Due to the high resistance that cancer has shown to conventional therapies, it is difficult to treat this disease, particularly in advanced stages. In recent decades, treatments have been improved, being more specific according to the characteristics of the tumor, becoming more effective, less toxic, and invasive. Cancer can be treated by the combi...

Impedance measurement is a common technique to characterize and detect the electrical properties of biological cells. However, to decode the underlying physical processes, it requires complex electrical models alongside prior knowledge of the sample under study. In this work, we introduce an attractive label-free method for sensing biological cells...

Nanoparticles (NPs) are novel platforms that can carry both cancer-targeting molecules and drugs to avoid severe side effects due to nonspecific drug delivery in standard chemotherapy treatments. Cancer cells are characterized by abnormal membranes, metabolic changes, the presence of lectin receptors, glucose transporters (GLUT) overexpression, and...

Background/aim: To determine the differential protein profiles of cervical cancer cell lines in order to find potential targets that can be used as biomarkers in low-grade squamous intraepithelial lesions (LSIL) diagnosis. Materials and methods: Proteomic analysis was performed on cervical cancer cell lines by 2D electrophoresis and liquid chrom...

Impedance measurement is a common technique to characterize and detect the electrical properties of biological cells. However, to decode the underlying physical processes, it requires complex electrical models alongside prior knowledge of the sample under study. In this work, we introduce an attractive label-free method for sensing biological cells...

Polyamidoamine dendrimers (PAMAM) have been extensively investigated for drug delivery in target therapy against breast cancer and other diseases, but the development of a low-cost system for target therapy, especially for triple-negative breast cancer (TNBC), remains limited. The objective of this study was to evaluate the novel one-step PAMAM den...

A series of highly potent antihyperlipidemic agents constituted by a fibrate-based structure was recently reported by our group, whose synthesis started from isovanillin derivatives. In the interest of evaluating the bioisosteric effect of the vanillin-based isomers on their antihyperlipidemic activity, the present study focuses on the synthesis of...

Three novel BODIPY-chromophores 4, 6 and 7 were synthesized by simple condensation reaction of thiocarboxaldehyde and pyrrole ring. Molecular structures were designed to contain thiophene rings and different arrangements for bromine substitution. The effect of these arrangements on the generation of singlet oxygen and optical properties was studied...

MnFe2O4 NPs are a current hot topic in materials science due to their high and tunable nanomagnetism, surface versatility and chemical stability, which makes them promising candidates for biomedical applications. This chapter aims to provide a concise review focused on the synthetic methods and surface engineering strategies for designing MnFe2O4 n...

Purpose In the present study, we investigated the effects of 17β-estradiol (E2) on membrane roughness and gold nanoparticle (AuNP) uptake in MCF-7 breast cancer cells. Methods Estrogen receptor (ER)-positive breast cancer cells (MCF-7) were exposed to bare 20 nm AuNPs in the presence and absence of 1×10⁻⁹ M E2 for different time intervals for up t...

Cervical intraepithelial neoplasia (CIN) are premalignant lesions, which can be low or high grade. The therapies used in Mexico for this alteration are invasive and deform the cervix causing fertility problems and recurrent abortions; for this reason, in this work, the use of Photodynamic Therapy (PDT) is proposed since it is directed to transforme...

The theragnosis combines functions of diagnosis, treatment and monitoring in real time through images, all with one pharmaceutical agent. IgM Antibody Anti Isoform 4 SOD Mitochondrial labeled with PpIX showed theragnosis activity in lung cancer cell line A-549 in previous work. The main objective of this work was evaluated the theragnostic activity...

Photodynamic therapy (PDT) is a non-conventional cancer treatment that consists in the administration of a photosensitizer (Ps) activated by light. The conjugate of protoporphyrin IX-gold nanoparticle used as Ps has showed an enhanced effect on cervical cancer cells treated with PDT. The aim of this work was to determinate the mechanisms involved i...

Nanotechnology in recent years, by using nanoparticles (Np) has emerged as an alternative diagnosis and treatment of breast cancer, being this the most common invasive cancer in women in the world. The most used treatment for this disease is chemotherapy, being highly toxic and unsafe causing adverse effects or death in patients. Dendrimeric Np hav...

Photodynamic therapy (PDT) is a new technique that has already been applied to several types of cancer, which combines a photosensitizer, light and oxygen. Interstitial PDT is based on intra-tumor light delivery using laser fibers inserted via needle. In this work, the fiber TF-N09 and the high-power laser system BIOSPEC® were tested in a syngeneic...

Aims: To study the potential genotoxicity of two polynuclear aromatic hydrocarbons (PAHs), exposed cultured human leukocytes in vitro using two types of biomarkers genotoxicity: DNA strand breaks (DNA-SB) and adduct formation (DNA-PAHs). Study Design: Human leukocytes were exposed to toxic cultures with different concentrations of anthracene (ANT),...

Background and objective Cervical cancer is an important health problem in our country. It is known that there are several risk factors for this neoplasm, and it has been suggested that cervical microbiome alterations could play a role in the development and progress of cancer. Bacterial vaginosis associated bacteria such as Atopobium vaginae and G...

Background and objective: Cervical cancer is an important health problem in our country. It is known that there are several risk factors for this neoplasm, and it has been suggested that cervical microbiome alterations could play a role in the development and progress of cancer. Bacterial vaginosis associated bacteria such as Atopobium vaginae and...

Background: In this work, a drug product composed of an IgM antibody derived from a hybridoma subclone 4C1F6D5G7B8 was prepared and further labeled with PpIX to be used in cell lines A-549 and MRC-5. The aim of this work was to evaluate the potential theranostic activity of the obtained product together with photodynamic therapy (PDT). Methods:...

The present study aimed to determine the effectiveness of photodynamic therapy (PDT), using δ-aminolevulinic acid (5-ALA), in the elimination of premalignant cervical lesions in Mexican patients with human papillomavirus (HPV) infection and/or cervical intraepithelial neoplasia (CIN). Thirty women diagnosed with CIN I and/or positive for HPV partic...

Soybeans are an important source of bioactive molecules, such as peptides, which generation can improve through germination. In this study, the antioxidant and antiproliferative activities of three peptide fractions (>10 kDa, 5–10 kDa and <5 kDa) that were obtained by ultrafiltration of soybean protein hydrolysate after six days of germination were...

Gold nanoparticles (AuNPs) have been proposed for use in the treatment of different types of cancer, including breast cancer. At present, neither the mechanisms of AuNP interaction with the plasma membrane surface and their delivery and intracellular distribution in cancer cells nor their effect on the plasma membrane so as to allow cell incorporat...

Ortho image assembled from Z-stack (A) (scale bar: 20 µm), present the place of incorporation of gold nanoparticles (AuNPs) into cell nucleus (crosslink section), after 6 hours of incubation. A 3D view of the ortho image is presented at (B) (scale bar: 20 µm). More details about the incorporated AuNPs can be seen in (C) and (D) on reconstructed ima...

Soybeans are an important source of bioactive molecules , such as peptides, which generation can improve through germination. In this study, the antioxidant and anti-proliferative activities of three peptide fractions (>10 kDa, 5-10 kDa and <5 kDa) that were obtained by ultrafiltration of soybean protein hydrolysate after six days of germination we...

Human pluripotent stem cells (hPSC) have promise for regenerative medicine due to their auto-renovation and differentiation capacities. Nevertheless, there are several ethical and methodological issues about these cells that have not been resolved. Human amniotic epithelial cells (hAEC) have been proposed as source of pluripotent stem cells. Severa...

Morphology of hAEC and hESC cultured in vitro and expression of pluripotency markers in H1 line. (A) Microphotography of hAEC at P0. (B) Typical colony morphology of the H1 line. (C) H1 cells used as positive control for our immunocytochemistry antibodies against: OCT4, SOX2 and NANOG (red) as well as SSEA4, TRA-1-60 and E-cadherin (green). Nuclei...

Data of the RT-qPCR of the relative expression and standard error of the pluripotent transcription factors for H9 and hAEC cells at different passages. (DOCX)

Somatic cells not express genes associated with pluripotency. Representative images of the electrophoresis of RT-PCR products of mRNAs for transcription factors OCT4 (151 bp), SOX2 (264 bp), NANOG (286 bp), REX1 (306 bp), KLF4 (134 bp) and GAPDH (229 pb) of mouse embryonic fibroblast (MEF) and human peripheral blood (PB). L = ladder, H9 = hESC line...

Background: Cancer testis antigens (CTA) are expressed in several types of cancer but not in normal adult tissue, with the exception of testicular germ cells, and for this reason are considered targets for immunotherapy. Objective: The aim of this study was to determine the frequency and clinical significance of CTA expression in cells from patient...

Este libro se puede adquirir en la librería del Instituto Politécnico Nacional Librería Tresguerras Tresguerras núm. 27 esq. Tolsá Col. Centro Histórico Deleg. Cuauhtémoc C. P. 06040, México, D. F. Horario: Lun. a Vie. de 9:00 a 18:00 hrs. Tel. (55) 5729 6300 ext. 65157 Correo: libreriatresguerras@ipn.mx

The photodynamic therapy (PDT) is a specific and alternative treatment for premalignant and malignant diseases. The α-Gal epitope is expressed in red blood cells and nucleated cells of placental mammals not primates, but absent in old world monkeys, apes and humans. α-Gal epitope has been found in cervical biopsies HPV positive, in biopsies at diff...

The unpredictable, evolutionary nature of the influenza A virus (IAV) is the primary problem when generating a vaccine and when designing diagnostic strategies; thus, it is necessary to determine the constant regions in viral proteins. In this study, we completed an in silico analysis of the reported epitopes of the 4 IAV proteins that are antigeni...

Photodynamic Therapy (PDT) is considered a highly effective method to diagnose and treat a wide variety of pre- malignant and malignant processes characterized by the selective elimination of cancerous cells. PDT is based in the capability of some neoplastic cells to specifically accumulate a photosensitizer (Ps), which is photoactivated with local...

Changes in the antioxidant capacity of albumin and alterations of the albumin structural conformation were examined in patients in advanced stages of diabetes nephropathy. Human serum albumin was purified from diabetic patients in pre-dialysis (glomerular filtration rate [GFR] between 15 and 29 ml min(-1) 1.73 m(-2)) and those in dialysis (GFR ≤ 15...

Nanotechnology is a promising interdisciplinary field for developing improved methods of diagnosis and treatment of different diseases, including cancer. Give their optical, magnetic, and structural property, the nanoparticles have been proposed to be use in the development of unconventional treatments for cancer such as photodynamic therapy (PDT)....

La infección por virus del papiloma humano (vph) en boca se asocia con lesiones como hiperplasia epitelial multifocal (hem), verruga vulgar (vv) y condiloma acuminado (ca). En este sentido, se evaluó la eficacia del diagnóstico clínico en lesiones por vph en boca y se propusieron criterios útiles para su diagnóstico. Los resultados sugieren que la...

Sistema para retener hidrocarburos arómaticos policiclos (HAP) de aires y su eliminación por fotocatalisis.

Objectives: To evaluate the effectiveness of Toki’s criteria in identifying the HPV L1 protein in oral lesions with the use of immunohistochemistry (IHC) and to determine which criteria optimize such identification. Study Design: Retrospective study of 277 cases diagnosed as HPV lesions at 22 years. Tests of sensitivity, specificity, positive pred...

http://www.pctnationalphaser.com/PCT_Patent_Publication/142014/WOMX2014051412/PCTMX2012000094

La presente invención describe un sistema matricial para irradiar cultivos celulares para pruebas in vitro de la terapia fotodinámica. El sistema matricial comprende un miembro de base, una pluralidad de diodos emisores de luz, una placa de irradiación, una pluralidad de pozos dispuestos de tal manera que forman una matriz de irradiación, un dispos...

Urban litter is generated by human societies everywhere. Some litter is recyclable waste. In this study, the acronym RMSW is used to refer to recyclable municipal solid waste generated in streets. Public attitude towards RMSW generation, generators’ perceptions, and quantification of RMSW in streets were examined in a Mexico City neighborhood, wher...

Previous studies showed that germination could improve the antiproliferative effect of soy protein on cervical cancer cells and that a peptide fraction (MAPF) from germinated soybeans decreases the expression of PTTG1 and TOP2A (2 genes considered as therapeutic targets) causing apoptosis of cancer cells. The aim of this work was to study the effec...

Cervical carcinoma (CC) represents the second leading cause of cancer death in Mexican women. No conventional treatments are being developed such as photodynamic therapy (PDT), involving the simultaneous presence of a photosensitizer (Ps), light of a specific wavelength and tissue oxygen. On the other hand, it has seen that the use of gold nanopart...

Each cigarette can generate 1149 ng of a mixture of 14 polycyclic aromatic hydrocarbons, of which there are a lot of information about its harmful effects on the environment and human health, they are considered mutagenic, teratogenic and carcinogenic. In this paper we tested ZnO:Mn2+ nanoparticles, attached to the filters of cigarettes. The first...

Protoporphyrin IX (PpIX) is a photosensitizer synthesized from 5-aminolevulinic acid (ALA) that has been used in photodynamic therapy (PDT) as a promising treatment for many types of cancer. In this work it was quantified the accumulation of PpIX in tumors and in different tissues of female mice (nu/nu) inoculated with breast cancer cells. Two rou...

Background: Oral lesions caused by human papillomavirus (HPV), share morphological features which may complicate their diagnosis. Aim: To analyze the usefulness of Toki's morphological criteria for the diagnosis of HPV lesions in cervix, in the diagnosis of oral lesions. Materials and methods: Retrospective study of 355 oral lesions by HPV. Th...

Background: Oral lesions caused by human papillomavirus (HPV), share morphological features which may complicate their diagnosis. Aim: To analyze the usefulness of Toki's morphological criteria for the diagnosis of HPV lesions in cervix, in the diagnosis of oral lesions. Materials and Methods: Retrospective study of 355 oral lesions by HPV. The pat...

In order to evaluate the improvement of the photodynamic therapy (PDT) due to sodium butyrate (NaBu), its effectiveness in U373-MG and D54-MG astrocytoma cell lines was evaluated. Cells were exposed to delta-aminolevulinic acid (δ-ALA) as a precursor to endogenous photosensitizer protoporphyrin IX (PpIX). In both astrocytoma cells, an important inc...

Thermal-diffusivity (D) and thermal-effusivity (e) measurements were carried out in l-cysteine nanoliquids l-cysteine in combination with Au nanoparticles and protoporphyrin IX (PpIX) nanofluid) by using thermal lens spectrometry (TLS) and photopyroelectric (PPE) techniques. The TLS technique was used in the two mismatched mode experimental configu...

The aim of this study was to evaluate the effect of a peptide fraction, obtained from a germinated soybean protein hydrolysate, on the viability, apoptosis and cancer related gene expression in HeLa cells. Soybean was germinated for 0-6 days and proteins were isolated from the seeds. Protein isolates, without ethanol-soluble phytochemicals, were hy...

Germination of soybeans increases the bioavailability of some nutrients. An evaluation was done to determine if germination increased the anti-adipogenic and lipolytic effects of soybean. Soybeans were germinated for 0 to 6 days and protein concentrates extracted from beans germinated at each period. Soy protein concentrates can retain notable amou...

Ditaxis heterantha seeds are used as spices for flavoring and coloring food. Two new apocarotenoids derived from the seeds, heteranthin and ditaxin, were evaluated for their in vitro cytotoxic effects in murine lymphoma cells lines. Bioabsorption in mice and preventive and antitumor effects of the apocarotenoids were determined. Ditaxin and heteran...

Cancer is one of the chronic diseases that has the highest incidence in the world. Extensive epidemiological, in vitro, and animal data suggest that soybean consumption reduces the risk of developing several types of cancer. To date, a number of nutrients and micronutrients with anticancer properties have been identified in soybean, including isofl...

In this chapter are shown some applications of two photothermal techniques (Photoacoustic Spectroscopy, PAS, and Thermal Lens Spectrometry, TLS) to determine the characteristic non-radiative relaxation time (NRRT) and the thermal diffusivity. In addition, complementary techniques as Fluorescence Spectroscopy, Optical Absorption Spectrometry and TEM...

This paper presents the development of an irradiation system for photodynamic therapy with temperature control. The system allows the application of an optical radiation whose wavelength is 630nm, using light-emitting high power diodes. The developed system has a power control to irradiate the target tissue with an adequate power density. A tempera...

Consumption of soybeans can reduce the risk of different types of cancer. Little is known about the effect of germination on the anticancer properties of soya. This study was done to determine if germination improves the anticancer properties of soybean protein through generation of amino acids or bioactive peptides. Soybean was germinated for 0, 2...

Congenital malformations are one of the major causes of child mortality all over the world. In order to prevent them it is necessary to find substances that act as anti-teratogenic agents. In this study hydroxyurea (HU), an antineoplastic and teratogenic drug, was administered to pregnant mice because one of its major mechanisms of teratogenesis is...

Primary osteoarthritis (OA) is a multifactorial disease with several genetics factors involved. The COL2A1 gene is of particular interest because it encodes for the most abundant protein in articular cartilage. The aim was to evaluate the association of COL2A1 gene polymorphism with OA of the knee in Mexican Mestizo patients. A case-control study w...

The damage induced by end products of photodynamic therapy (PDT) in astrocytoma tumors leads to cytotoxicity and cell death. Chromatin modifiers such as sodium butyrate (NaB) induce several genes involved in apoptosis, among others. The PDT improvement was evaluated by the measurement of its effectiveness in the treatment of U373-MG and D54-MG astr...

In the present study we extracted three isomeric acetogenins (Ace) from the seeds of Annona cherimolia Mill. (Annonaceae) and determined their genotoxic and cytotoxic potential in mice. Our results showed a significant increase in the rate of micronucleated polychromatic erythrocytes induced by Ace with respect to the value for the control group; t...

In this work we have used the Photoacoustic Spectroscopy (PAS) to determine in vitro the non-radiative relaxation time (NRRT) of a protoporphyrin IX (PpIX) standard solution and samples of PpIX(1), PpIX(2) and PpIX(3) with Au nanoparticle concentrations of 0.001008, 0.00504 and 0.01008 mmol in 25 mL of water respectively. We have used PpIX disodium...

In this work, it is reported the gold nanoparticles synthesis, their characterization, and their application to the enhancement of the thermal transport in a cellular culture medium. The Au nanoparticles (NPs), with average size of 10 nm, contained into a culture medium (DMEM (1)/F12(1)) (CM) increased considerably the heat transfer in the medium....

Inorganic urban solid waste (IUSW) is a serious problem in developing countries, and IUSW in the street that does not have adequate final disposal is responsible for serious environmental effects. The aim of this work was to determine the dynamics of the generation of IUSW in the streets of two neighborhoods of different socioeconomic strata in Mex...

The aim of this work was to evaluate the induction of protoporphyrins IX (PpIX) activity and superoxide anions (SO) in human leukocytes exposed to anthracene (ANT) and benzo(a)pyrene (B(a)P). The leukocyte LC(50)s for both hydrocarbons and the PpIX accumulation and SO overproduction were measured. The LC(50)s were 0.35 and 3.23μM for ANT and B(a)P,...

The increasing incident of resistant strains to antibiotic has encouraged the search of new antibacterial treatments, such as the photodynamic therapy. In recent years, photodynamic therapy has demonstrated being a good technology for the treatment of recurrent bacteria infection. PDT presents a hopeful approach to eliminate Gram positive and negat...

The use of gold nanoparticles (np‐Au) to eliminate cancer has proved to be very effective due to the fact that cancerous cells accumulate it 600% more than healthy cells. In addition they have a high capacity of absorption and dispersion of light. Therefore, the effectiveness of photodynamic therapy (PDT) could be improved by the simultaneous use...

Early detection of skin cancer is fundamental to a successful treatment. Changes in the shape, including the relief, of skin lesions are an indicator of a possible malignity. Optical microtopographic inspection of skin lesions can be used to identify diagnostic patterns of benign and malign skin' lesions. Statistical parameters like the mean roughn...

The present study examines the relationships between cytochrome P4501A (CYP1A) activity and vitellogenin (VTG) induction in Ameca splendens elicited by a polychlorinated biphenyl (PCB) mixture. Ethoxyresorufin-O-deethylase (EROD) activity, mRNA levels of VTG, and VTG induction were evaluated in male and female fish exposed for 1, 2, 4, 8, and 16 d...

. In order to compare the non radiative relaxation time (NRRT) between standard protoporphyrin IX (PpIX) and protoporphyrin PpIX(1) solution containing gold metallic nanoparticles, we measured the photoacoustic spectroscopy (PAS) signal phase to determine, for each solution, the NRRT by using the Rosencwaig-Gesho theory, modified to include t...

The present study examines the relationships between cytochrome P450 1A (CYP1A) activity and vitellogenin (VTG) induction in Ameca splendens elicited by a polychlorinated biphenyl (PCB) mixture. Ethoxyresorufin-O-deethylase (EROD) activity, mRNA levels of VTG and VTG induction were evaluated in male and female fish exposed for 1, 2, 4, 8 and 16 d t...

Photodynamic therapy (PDT), using protoporphyrin IX (PpIX) as a natural photosensitizer, may be a viable alternative therapy of retinoblastoma. In order to evaluate the potential value of PpIX, the expression profiles of genes involved in heme biosynthesis in human retinoblastoma WERI-Rb-1 and Y79 cells were analyzed by reverse transcriptase-polyme...

Girardinichthys viviparus is a Mexican endangered endemic fish species living only in Lake Texcoco (LTX), one of two extant type localities for this species. The other type locality is Lake Zumpango (LZ). LTX and LZ are fed by wastewater treated at secondary level that contains several endocrine disrupting chemicals. Our goal was to assess the estr...