Eva Hadadi

Vrije Universiteit Brussel | VUB

Monocytes play a central role in regulating inflammation in response to infection or injury, and during auto-inflammatory diseases. Human blood contains classical, intermediate and non-classical monocyte subsets that each express characteristic patterns of cell surface CD16 and CD14; each subset also has specific functional properties, but the mech...

Background and aims Lipid-rich foam cell macrophages drive atherosclerosis via several mechanisms, including inflammation, lipid uptake, lipid deposition and plaque vulnerability. The atheroma environment shapes macrophage function and phenotype; anti-inflammatory macrophages improve plaque stability while pro-inflammatory macrophages promote ruptu...

Breast cancer is the most common type of cancer worldwide and one of the major causes of cancer death in women. Epidemiological studies have established a link between night-shift work and increased cancer risk, suggesting that circadian disruption may play a role in carcinogenesis. Here, we aim to shed light on the effect of chronic jetlag (JL) on...

Local delivery of mRNA-based immunotherapy offers a promising avenue as it enables the production of specific immunomodulatory proteins that can stimulate the immune system to recognize and eliminate cancer cells while limiting systemic exposure and toxicities. Here, we develop and employ lipid-based nanoparticles (LNPs) to intratumorally deliver a...

Classical monocytes are recruited to tumors and undergo transcriptional reprogramming resulting in tumor-promoting functions. Epigenomic features, such as post-translational modification of histones and chromatin accessibility, are key determinants of transcription factor binding and thereby play an important role in determining transcriptional res...

Today's challenge for precision medicine involves the integration of the impact of molecular clocks on drug pharmacokinetics, toxicity, and efficacy, toward personalized chronotherapy. Meaningful improvements of tolerability and/or efficacy of medications through proper administration timing have been confirmed over the past decade for immunotherap...

Agonistic αCD40 therapy has been shown to inhibit cancer progression in only a fraction of patients. Understanding the cancer cell-intrinsic and microenvironmental determinants of αCD40 therapy response is therefore crucial to identify responsive patient populations and to design efficient combinatorial treatments. Here, we show that the therapeuti...

Macrophages are diverse immune cells populating all tissues and adopting a unique tissue-specific identity. Breast macrophages play an essential role in the development and function of the mammary gland over one's lifetime. In the recent years, with the development of fate-mapping, imaging and scRNA-seq technologies we grew a better understanding o...

Agonistic αCD40 therapy has shown to inhibit cancer progression, but only in a fraction of patients. Hence, understanding the cancer cell-intrinsic and microenvironmental determinants of αCD40 therapy response is crucial to identify responsive patient populations and design efficient combination treatments. Here, we showed that the therapeutic effi...

Epithelial-Mesenchymal Transition (EMT) and its reciprocal Mesenchymal-Epithelial Transition (MET) occur naturally as a cycling process during embryonic and foetal development. The capacity of such iterative cycles to drive cell fate and cellular and molecular behaviour in physiology or pathology remains unclear. We describe here a protocol to indu...

The circadian clock coordinates biological and physiological functions to day/night cycles. The perturbation of the circadian clock increases cancer risk and affects cancer progression. Here, we studied how BMAL1 knockdown (BMAL1-KD) by shRNA affects the epithelial–mesenchymal transition (EMT), a critical early event in the invasion and metastasis...

The circadian rhythm is a major environmental regulator of plants and animal physiology. The alternation of days and nights is translated at the cell and tissue level thanks to a molecular machinery, called the circadian clock. This clock controls in particular numerous endocrine functions and its imbalances can have serious consequences on homeost...

Epithelial-Mesenchymal Transition (EMT) and its reciprocal Mesenchymal-Epithelial Transition (MET) occur naturally as a cycling process during embryonic and foetal development. The capacity of such iterative cycles to drive cell fate and cellular and molecular behaviour in physiology or pathology remains unclear. We describe here a protocol to indu...

Breast cancer is the most common type and one of the major causes of cancer death in woman worldwide. Epidemiological studies have established a link between night shift work and increased cancer risk, suggesting that circadian disruption may interfere with carcinogenesis. We aim to shed light on the effect of chronic jetlag on mammary tumour devel...

The circadian clock controls most of the physiological processes in the body throughout days and nights’ alternation. Its dysregulation has a negative impact on many aspects of human health, such as obesity, lipid disorders, diabetes, skin regeneration, hematopoiesis and cancer. To date, poor is known on the molecular mechanisms that links mammary...

Chalcones present in edible plants possess anti-cancer and anti-inflammatory properties, with the Michael acceptor moiety reported to be responsible for their biological activities. In this study, two novel dihydrotriazine-chalcone compounds previously identified to exert anti-proliferative effects through dual-targeting of dihydrofolate reductase...

Human primary monocytes comprise a heterogeneous population that can be classified into three subsets based on CD14 and CD16 expression: classical (CD14high/CD16-), intermediate (CD14high/CD16+), and non-classical (CD14low/CD16+). The non-classical monocytes are the most pro-inflammatory in response to TLR stimulation in vitro, yet they express a r...

This corrects the article DOI: 10.1038/srep27059.

Everolimus (EV), a rapamycin analogue mTOR inhibitor, is used in the clinic to treat Estrogen positive (ER⁺) breast cancer in order to avoid the resistance to hormonotherapy. Here, we investigated whether EV efficacy varied according to administration timing by using the ER⁺ breast cancer cell line MCF-7 as model system. Our results showed that ins...

Introduction Genome wide association studies have identified Tribbles-1 (TRIB1) to be significantly associated with all major plasma lipid traits and as a risk factor for ischaemic heart disease and myocardial infarction. Studies in mice using Trib1 full body KO and liver-specific over-expression and KO models have shown that hepatic expression of...

Rationale Cardiovascular disease is the leading cause of death world–wide. Macrophages are crucial in regulating the plaque environment, especially the lipid content. However, current characterisation of macrophage phenotypes lipoprotein handling capacity is conflicting and incomplete. We hypothesised that lipoprotein handling differed among distin...

The pig is an emerging animal model, complementary to rodents for basic research and for biomedical and agronomical purposes. However despite the progress made on mouse and rat models to produce genuine pluripotent cells, it remains impossible to produce porcine pluripotent cell lines with germline transmission. Reprogramming of pig somatic cells u...

The hypothalamic-pituitary-thyroid (HPT) axis is governed by hypophysiotropic thyrotropin-releasing hormone (TRH)-synthesizing neurons located in the hypothalamic paraventricular nucleus (PVN) under control of the negative feedback of thyroid hormones. The mechanisms underlying the ontogeny of this phenomenon are poorly understood. We aimed to dete...

sec> Introduction Cardiovascular disease, especially atherosclerosis and related pathologies, is one of the leading causes of death globally. It is well established that macrophages play a crucial role in the formation and progression of atherosclerotic plaques. Macrophages are very plastic and can alter their phenotypes and functions according to...

Purpose Based on a previous gene expression study in a mouse model of asthma, we selected 60 candidate genes and investigated their possible roles in human asthma. Methods In these candidate genes, 90 SNPs were genotyped using MassARRAY technology from 311 asthmatic children and 360 healthy controls of the Hungarian (Caucasian) population. Moreove...

Introduction Cardiovascular disease, especially atherosclerosis and related pathologies, is one of the leading causes of death globally. It is well established that macrophages play crucial role in the formation and progression of atherosclerotic plaques. Macrophages are very plastic and can alter their phenotypes and functions according to their e...

The primary objective of this study was to perform new, relevant information about cranial suture closure in adults. Single nucleotide polymorphisms (SNPs) in targeted genes were examined, which encode factors that play an important role in cranial suture development and maintenance. Our hypothesis was that some of these genes and polymorphisms can...

Objectives: The role was studied of multiple single nucleotide polymorphisms in tooth agenesis in the Hungarian population using a complex approach. Methods: Eight SNPs, PAX9 -912 C/T, PAX9 -1031 A/G, MSX1 3755 A/G, FGFR1 T/C rs881301, IRF6 T/C rs764093, AXIN2-8150 A/G, AXIN2-8434 A/G and AXIN2-30224 C/T, were studied in 192 hypodontia and 17 ol...

Introduction Mitogen activated protein kinase (MAPK) cascades are involved in the regulation of several cellular responses including migration or proliferation. We have shown previously that tribbles-1 (trb-1), a member of the tribbles family of adaptor/scaffold proteins controls vascular smooth muscle proliferation and chemotaxis via a direct inte...

Introduction Cardiovascular disease such as atherosclerosis is currently the leading cause of death by noncommunicable diseases worldwide. Development of atherosclerosis which is considered a chronic inflammatory disease has been associated with a number of pro-inflammatory cytokines including interleukin-1 (IL-1). IL-1 has been associated with ath...

Background: Histamine as an inflammatory mediator plays an important role in chronic allergic and asthmatic conditions. However, the role of genetic polymorphisms of the histamine receptor HRH4 (histamine receptor H4) gene in asthma susceptibility and endophenotypes has not been studied yet. Our aim was to investigate the possible association betw...

Genetic studies indicate high number of potential factors related to asthma. Based on earlier linkage analyses we selected the 11q13 and 14q22 asthma susceptibility regions, for which we designed a partial genome screening study using 145 SNPs in 1201 individuals (436 asthmatic children and 765 controls). The results were evaluated with traditional...

Haplotype blocks of the investigated SNPs in the present study. SNPs are numbered sequentially and their relative location is indicated along the top. Markers 1–54 and markers 55–102 correspond to the studied SNPs on Chromosome 14 and 11, respectively. Triangles surrounding markers represent haplotype blocks. (TIF)

Comparison of standard concept of (pairwise) association and strong relevance. The concept of strong relevance and association (with respect to a target) has only one common element, direct relevance, i.e. non-mediated relationship between the target and a variable. Association also includes confounded and transitive relevance, where there is a med...

The most probable univariate (MBM), bivariate (2-MBS), trivariate (3-MBS), 4-MBS, 5-MBS subsets of variables. All posteriors of partial k-relevance are ordered, x axis denotes the rank (e.g.: the number 2 means the second highest posterior), and y axis denotes the posterior probabilities. As k increases (i.e. the set size of jointly considered SNPs...

Information on the examined SNPs. (XLS)

The peakness of the posteriors of the most probable 100 MBS sets. The x axis denotes the rank of a Markov blanket set (MBS) of SNPs, the y axis denotes the joint probability of an MBS, e.g. an MBS with rank = 5 is the fifth most probable set. The “RA” and “CLI” prefixes denote the corresponding dataset, and the “asthma” and “multitarget” suffixes i...

Minor allele and genotype frequencies (%) in asthmatic (n = 436) and control (n = 765) patients. (DOC)

Statistical evaluation for association of SNPs with asthma at allele and genotype levels. (DOC)

In the last few years, it has been recognized that the unbalanced regulation of survival and apoptosis of bronchial inflammatory cells is a key component in the development of asthma. Baculoviral IAP repeat containing 5 (BIRC5) (also known as survivin) is an important anti-apoptotic protein that has been implicated in many cancer types, and recent...

Air pollution and subsequent increased oxidative stress have long been recognized as contributing factors for asthma phenotypes. Individual susceptibility to oxidative stress is determined by genetic variations of the antioxidant defence system. In this study, we analysed the association between environmental nitrogen dioxide (NO(2)) exposure and s...

Analysis of mitochondrial mutations in the HVS-I region is an effective method for ancient human populational studies. Discontinuous haplotype data between the first farmers and contemporary Europeans has been described before. Our contribution is based on a survey initiated on the Neolithic skeletons from Hungarian archaeological sites in the Alfö...

Objectives: Genes regulating tooth development have been studied actively and to date over 300 genes have been associated with patterning, morphogenesis and cell differentiation in teeth. Majority of these genes are signaling pathways mediating cellular communication between epithelial and mesenchimal tissues and mutations cause dental defects. As...