Eva Barreno

University of Valencia | UV · Instituto Cavanilles de Biodiversidad y Biología Evolutiva

Ricasolia virens es un hongo liquenizado epífito encontrado predominantemente en Europa Occidental y Macaronesia, que prospera en bosques bien estructurados con continuidad ecológica y bajos niveles de eutrofización. Según las categorías de la UICN, se considera amenazado o extinto en muchas regiones de Europa. Sus talos son tripartitos, con el mic...

Trebouxiophyceae are particularly widespread in terrestrial environments and comprise most of the lichen-forming microalgae genera. These symbionts have been frequently considered asexual, however, their life cycles remain largely unknown. We sequenced and analysed the nuclear genome of Trebouxia lynniae, a model Trebouxiophyceae phycobiont, monito...

Ramalina farinacea is a widely distributed epiphytic lichen from the Macaronesian archipelagos to Mediterranean and Boreal Europe. Previous studies have indicated a specific association between R. farinacea and Trebouxia microalgae species. Here, we examined the symbiotic interactions in this lichen and its closest allies (the so-called “R. farinac...

The lichen Ricasolia virens (With.) H.H. Blom. & Tønsberg has a wide geographical distribution in Europe, and it is considered (Red Lists, IUCN) one of the best bioindicators of mature forests, which have ecological continuity and no relevant anthropogenic impacts. However, its biology, ecology and distribution have been scarcely studied. R. virens...

Principal del Proyecto PROMETEO 2021/005 CERTIFICA Que JUAN S. ÁLVAREZ ASPRA amb DNI 09406872X ECOPHYSIOLOGY AND GENOMICS APPLIED TO ha participat amb la ponència: Development of a predictive model to estimate populations of the endangered lichen Ricasolia virens in the Sierra del Sueve (Asturias, Spain) dins del marc del WORKSHOP Ecophysiology and...

In the 1990s, a sampling network for the biomonitoring of forests using epiphytic lichen diversity was established in the eastern Iberian Peninsula. This area registered air pollution impacts by winds from the Andorra thermal power plant, as well as from photo-oxidants and nitrogen depositions from local and long-distance transport. In 1997, an ass...

An increasing number of studies are describing the diversity of lichen phycobionts, which is leading to a better understanding of how lichen communities are assembled at different taxonomic, evolutionary and geographical scales. The present study explores the identity and genetic diversity of the microalgal partners of Punctelia borreri and P. subr...

Juan S. Álvarez-Aspra, Eva Barreno The lichen Ricasolia virens has a wide geographical distribution in Europe, and is considered (Red Lists, IUCN) one of the best bioindicators of mature forests, with ecological continuity, without significant anthropogenic impacts. However, the biology and ecology of its thalli and populations have been scarcely...

Ricasolia virens is an epiphytic lichen-forming fungus mainly distributed in Western Europe and Macaronesia in well-structured forests with ecological continuity that lack eutrophication. It is considered to be threatened or extinct in many territories in Europe (IUCN). Despite its biological and ecological relevance, studies on this taxon are scar...

Based on molecular and morphological evidence, a new species within Verrucariaceae, Clavascidium hispaniam-pakistanica is described from Pakistan and Spain. The taxon is characterized by thicker and slightly larger squamules with a mixed-type medulla. Additionally, it has marginal pycnidia with bacilliform conidia. We have also detected the microal...

The fungal mitochondrial small subunit (mtSSU) ribosomal DNA is one of the most commonly used loci for phylogenetic analysis of lichen-forming fungi, but their primer specificity to mycobionts has not been evaluated. The current study aimed to design mycobiont-specific mtSSU primers and highlights their utility with an example from the saxicolous l...

Ramalina farinacea is an epiphytic lichen-forming fungus with a broad geographic distribution, especially in the Northern Hemisphere. In the eighties of the last century, it was hypothesized that R. farinacea had originated in the Macaronesian–Mediterranean region, with the Canary Islands as its probable southernmost limit, and thereafter it would...

Lichen phycobiomes have recently emerged as a source of biodiversity and new species of microalgae. Although in the genus Diplosphaera free-living microalgae are common, numerous strains belonging to this genus have frequently been recognized or isolated from lichen thalli. In this study, a comprehensive analysis of the strain Diplosphaera sp. ASUV...

The lichenized green microalga Trebouxia lynnae Barreno has been recently described and is considered a model organism for studying lichen chlorobionts. Its cellular ultrastructure has already been studied in detail by light, electron, and confocal microscopy, and its nuclear, chloroplast and mitochondrial genomes have been sequenced and annotated....

Near-infrared spectroscopy (NIRS) is an accurate, fast and safe technique whose full potential remains to be exploited. Lichens are a paradigm of symbiotic association, with extraordinary properties, such as abiotic stress tolerance and adaptation to anhydrobiosis, but subjacent mechanisms await elucidation. Our aim is characterizing the metabolomi...

The term phycobiome was recently introduced to designate all the microalgae (primary or non-primary) associated with lichen symbioses. Abundant non-primary symbiotic microalgae are usually obtained from lichen isolations, confirming that thalli are a source of biodiversity and new species. In this study, microalgae were isolated from thalli of Buel...

Two microalgal species, Trebouxia jamesii and Trebouxia sp. TR9, were detected as the main photobionts coexisting in the thalli of the lichen Ramalina farinacea. Trebouxia sp. TR9 emerged as a new taxon in lichen symbioses and was successfully isolated and propagated in in vitro culture and thoroughly investigated. Several years of research have co...

Ramalina farinacea, a lichen-forming fungus with a vast geographical spread across the Mediterranean, Temperate, and Boreal bioclimates, has showcased intriguing variations in its symbiotic relationships with phycobionts. Our study aimed to elucidate these fungal-algal partnerships across a latitudinal stretch from Algeria (36oN) to Umeå, Sweden (6...

Coccoid microalgae of the genus Trebouxia Puymaly are by far the most prevalent among the various species involved in lichen symbioses. However, their taxonomic knowledge is rather scarce compared to that of lichenized fungi. In the present work, a taxonomic study integrating diverse techniques (phylogenetics, light, confocal and transmission elect...

Fungal–algal relationships—both across evolutionary and ecological scales—are finely modulated by the presence of the symbionts in the environments and by the degree of selectivity and specificity that either symbiont develop reciprocally. In lichens, the green algal genus Trebouxia Puymaly is one of the most frequently recovered chlorobionts. Treb...

The decline observed in many populations of highly sensitive lichens has led to the inclusion of some species on regional Red Lists, but this has seldom been accompanied by an examination of the genetic variation of the fungal and algal symbiotic partners. Here, the red-listed epiphytic lichen Seirophora villosa was surveyed in 14 Mediterranean coa...

The worldwide, ecologically relevant lichen-forming genus Parmelia currently includes 41 accepted species, of which the Parmelia sulcata group (PSULgp) and the Parmelia saxatilis group (PSAXgp) have received considerable attention over recent decades; however, phycobiont diversity is poorly known in Parmelia s. lat. Here, we studied the diversity o...

https://authors.elsevier.com/c/1e5F~7sxn0bzuT Trebouxiophyceae is a wide class of green algae comprising coccoid and elliptic unicells, filaments, blades and colony-forming species that occur in diverse terrestrial and aquatic environments. Within this class, the genus Trebouxia Puymaly is among the most widespread lichen phycobionts worldwide. How...

Morphology of Watanabea lichenicola Chiva, Dumitru, Bordenave & Barreno isolated from Buellia zoharyi lichen species. Left, micrograph of mature cells by transmission electron microscopy; upper right, light micrograph of W. lichenicola; Lower right, confocal reconstruction of mature chloroplast.

New resources of food, pharmaceuticals or biotechnological products are needed. The huge biodiversity of aero-terrestrial lichen-symbiont microalgae belonging to the Chlorophyta group remains unexplored despite they present interesting features such as extreme stress tolerance and growth in water shortage. Appropriateness for human consumption dema...

Microalgae of the genus Watanabea are widely distributed as free living in soil/subaerial and aquatic habitats. In this study, two Watanabea spp. were isolated from lichen thalli of Buellia zoharyi collected on biocrusts in the Iberian Peninsula and the Canary Islands. To ascertain their taxonomic position and phylogenetic relationships within the...

"Colección de Microalgas Simbiontes de Líquenes-Ficobiontes" "Collection of Symbiotic Microalgae of the University of Valencia", ASUV (www.asuvalgae.com) The "Lichen Symbiogenesis" research team has developed the "Collection of Symbiotic Microalgae of the University of Valencia", ASUV (www.asuvalgae.com), through the design of original protocols...

Buellia zoharyi is a crustose placodioid lichen, usually occurring on biocrusts of semiarid ecosystems in circum-Mediterranean/Macaronesian areas. In previous work, we found that this lichenized fungus was flexible in its phycobiont choice in the Canary Islands. Here we test whether geography and habitat influence phycobiont diversity in population...

New resources of food, pharmaceuticals or biotechnological products are needed. The huge biodiversity of aero-terrestrial lichen-symbiont microalgae remains unexplored. Viability of these for human consumption demands the demonstration of the absence of toxic effects. In vitro biocompatibility of crude homogenates of axenic microalga Asterochloris...

Lichen symbioses are microecosystems hosting many other living organisms besides the two major lichen symbionts (i.e., lichenized fungi [the mycobiont] and green microalgae or cyanobacteria [the photobiont]). Recent investigations evidenced that other fungi, non-photosynthetic bacteria and microalgae co-inhabit within the lichen thalli, but their d...

This study analyses the interactions among crustose and lichenicolous lichens growing on gypsum biocrusts. The selected community was composed of Acarospora nodulosa, Acarospora placodiiformis, Diploschistes diacapsis, Rhizocarpon malenconianum and Diplotomma rivas-martinezii. These species represent an optimal system for investigating the strategi...

Abstract Lichens provide valuable systems for studying symbiotic interactions. In lichens, these interactions are frequently described in terms of availability, selectivity and specificity of the mycobionts and photobionts towards one another. The lichen-forming, green algal genus Trebouxia Puymaly is among the most widespread photobiont, associati...

Lichens undergo desiccation/rehydration cycles and are permeable to heavy metals, which induce free radicals. Nitrogen monoxide (NO) regulates important cellular functions, but the research on lichen NO is still very scarce. In Ramalina farinacea thalli, NO seems to be involved in the peroxidative damage caused by air pollution, antioxidant defence...

Lichens provide valuable systems for studying symbiotic interactions. In lichens, these interactions are frequently described in terms of availability, selectivity and specificity of the mycobionts and photobionts towards one another. The lichen-forming, green algal genus Trebouxia Puymaly is among the most widespread photobiont, associating with a...

The Trebouxiophyceae is the class of Chlorophyta algae from which the highest number of chloroplast genome (cpDNA) sequences have been obtained. Several species in this class participate in symbioses with fungi to form lichens. However, no cpDNA has been obtained from any Trebouxia lichen‐symbiont microalgae, which are present in approximately half...

Lichens are symbiotic organisms, originated by mutualistic associations of heterotrophic fungi (mycobiont), photosynthetic partners (photobionts) which can be either cyanobacteria (cyanobionts) or green microalgae (phycobionts), and bacterial consortia. They are poikilohydric organisms without cuticles or nutrient absorption organs adapted to anhyd...

Lichens are poikilohydrous symbiotic associations between a fungus, photosynthetic partners, and bacteria. They are tolerant to repeated desiccation/rehydration cycles and adapted to anhydrobiosis. Nitric oxide (NO) is a keystone for stress tolerance of lichens; during lichen rehydration, NO limits free radicals and lipid peroxidation but no data o...

Trebouxiophyceae (Chlorophyta) is a species-rich class of green algae with a remarkable morphological and ecological diversity. Currently, there are a few completely sequenced mitochondrial genomes (mtDNA) from diverse Trebouxiophyceae but none from lichen symbionts. Here, we report the mitochondrial genome sequence of Trebouxia sp. TR9 as the firs...

Lichens are complex symbiotic systems, in which coexistence occurs between several microalgal taxa and/or lineages with a single fungus-mycobiont-, displaying different tolerance patterns to multiple kinds of abiotic stress. Furthermore, certain communities of non-photosynthetic bacteria and yeasts are starting to be considered as an integral part...

A taxonomical system for Trebouxia microalgae based on ultrastructural studies of Buellia zoharyi Galun and its subsequent statistical analysis is proposed in this study. Four different Trebouxia morphotypes were found in different areas of this lichen species. We needed to establish a taxonomical system allowing us to precisely classify these mor...

Tolerance to saline conditions has been thoroughly studied in plants and, to an extent, freeliving microalgae. However, scientific data regarding salt stress on symbiotic lichen microalgae is scarce to non-existent. Since lichen phycobionts are capable of enduring harsh, restrictive and rapidly changing environments, it is interesting to study the...

Main conclusion: For the first time we provide a study on the physiological, ultrastructural and molecular effects of salt stress on a terrestrial symbiotic green microalga, Trebouxia sp. TR9. Although tolerance to saline conditions has been thoroughly studied in plants and, to an extent, free-living microalgae, scientific data regarding salt stre...

This meeting is dedicated to discussing and better clarifying our knowledge of the complex interactions that uphold lichen symbiosis, through the characterization of different aspects of biology and phylogenetic relationships within the symbionts. Outstanding international attendees experienced in different research field of lichen symbiosis will p...

Phycologia: 2018, Vol. 57, No. 5, pp. 503-524. www.phycologia.org https://doi.org/10.2216/17-134.1 The class Trebouxiophyceae is comprised of coccoid to ellipsoid unicells, filaments, blades and colony-forming species of green algae occurring in diverse terrestrial and aquatic environments. Some representatives have evolved parasitic heterotrophic...

https://onlinelibrary.wiley.com/doi/abs/10.1111/jpy.12751. https://rdcu.be/0FTE. Three vagrant (Circinaria hispida, Circinaria gyrosa, Circinaria sp. ‘paramerae’) and one crustose (semi‐vagrant, Circinaria sp.’oromediterranea’) lichens growing in very continental areas in the Iberian Peninsula were selected to study the phycobiont diversity. Myc...

Intrathalline phycobiont diversity was investigated in a rosette-forming lichen, Parmotrema pseudotinctorum , using a combination of Sanger sequencing, 454-pyrosequencing, conventional light and confocal microscopy, and transmission electron microscopy. A total of 39 thalli sampled in five Canary Island populations were investigated. Three novel li...

Ana Crespo: a 70th birthday tribute - Volume 50 Issue 3 - Pradeep K. DIVAKAR, Eva BARRENO, Leopoldo SANCHO, H. Thorsten LUMBSCH

Myrmecia israeliensis has been traditionally considered as a green coccoid free–living microalga. This microalga was previously suggested as the primary phycobiont in the lichens Placidium spp., Heteroplacidium spp., and Psora decipiens. However, due to the absence of ITS rDNA sequences (barcode information) published along with these investigation...

Lichen thalli represent the most conspicuous examples of fungal-algal interactions. Studies that describe phycobiont diversity within entire thalli are based mainly on Sanger sequencing. In some lichen species, this technique could underestimate the intrathalline coexistence of multiple microalgae. In this study different multi-tool approaches were...

Myrmecia israelensis (S.Chantanachat & H.Bold) T.Friedl has been traditionally considered as a green coccoid free-living microalga. This microalga was suggested as the primary phycobiont in two related lichen genera Placidium and Heteroplacidium, and recently in Psora decipiens. However, due to the absence of nrITS DNA sequences (barcode informatio...

Biological Soil Crusts (BSCs) are biologically modified soil surfaces composed of a combination of organisms which include lichens, bryophytes, liverworts, microalgae, cyanobacteria, and microfungi. BSCs are complex communities due to the many different organisms involved and functional aspects they provide. Buellia zoharyi Galun is a prevailing li...