Eva Barreno

Eva Barreno
University of Valencia | UV · Instituto Cavanilles de Biodiversidad y Biología Evolutiva

Emeritus Professor in Botany

About

372
Publications
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August 1986 - present
Universitat de València

Publications

Publications (372)
Article
Full-text available
Fungal–algal relationships—both across evolutionary and ecological scales—are finely modulated by the presence of the symbionts in the environments and by the degree of selectivity and specificity that either symbiont develop reciprocally. In lichens, the green algal genus Trebouxia Puymaly is one of the most frequently recovered chlorobionts. Treb...
Article
The decline observed in many populations of highly sensitive lichens has led to the inclusion of some species on regional Red Lists, but this has seldom been accompanied by an examination of the genetic variation of the fungal and algal symbiotic partners. Here, the red-listed epiphytic lichen Seirophora villosa was surveyed in 14 Mediterranean coa...
Article
Full-text available
The worldwide, ecologically relevant lichen-forming genus Parmelia currently includes 41 accepted species, of which the Parmelia sulcata group (PSULgp) and the Parmelia saxatilis group (PSAXgp) have received considerable attention over recent decades; however, phycobiont diversity is poorly known in Parmelia s. lat. Here, we studied the diversity o...
Article
https://authors.elsevier.com/c/1e5F~7sxn0bzuT Trebouxiophyceae is a wide class of green algae comprising coccoid and elliptic unicells, filaments, blades and colony-forming species that occur in diverse terrestrial and aquatic environments. Within this class, the genus Trebouxia Puymaly is among the most widespread lichen phycobionts worldwide. How...
Cover Page
Full-text available
Morphology of Watanabea lichenicola Chiva, Dumitru, Bordenave & Barreno isolated from Buellia zoharyi lichen species. Left, micrograph of mature cells by transmission electron microscopy; upper right, light micrograph of W. lichenicola; Lower right, confocal reconstruction of mature chloroplast.
Article
Full-text available
New resources of food, pharmaceuticals or biotechnological products are needed. The huge biodiversity of aero-terrestrial lichen-symbiont microalgae belonging to the Chlorophyta group remains unexplored despite they present interesting features such as extreme stress tolerance and growth in water shortage. Appropriateness for human consumption dema...
Article
Full-text available
Microalgae of the genus Watanabea are widely distributed as free living in soil/subaerial and aquatic habitats. In this study, two Watanabea spp. were isolated from lichen thalli of Buellia zoharyi collected on biocrusts in the Iberian Peninsula and the Canary Islands. To ascertain their taxonomic position and phylogenetic relationships within the...
Chapter
Full-text available
"Colección de Microalgas Simbiontes de Líquenes-Ficobiontes" "Collection of Symbiotic Microalgae of the University of Valencia", ASUV (www.asuvalgae.com) The "Lichen Symbiogenesis" research team has developed the "Collection of Symbiotic Microalgae of the University of Valencia", ASUV (www.asuvalgae.com), through the design of original protocols...
Article
Full-text available
Buellia zoharyi is a crustose placodioid lichen, usually occurring on biocrusts of semiarid ecosystems in circum-Mediterranean/Macaronesian areas. In previous work, we found that this lichenized fungus was flexible in its phycobiont choice in the Canary Islands. Here we test whether geography and habitat influence phycobiont diversity in population...
Preprint
Full-text available
New resources of food, pharmaceuticals or biotechnological products are needed. The huge biodiversity of aero-terrestrial lichen-symbiont microalgae remains unexplored. Viability of these for human consumption demands the demonstration of the absence of toxic effects. In vitro biocompatibility of crude homogenates of axenic microalga Asterochloris...
Article
Full-text available
Lichen symbioses are microecosystems hosting many other living organisms besides the two major lichen symbionts (i.e., lichenized fungi [the mycobiont] and green microalgae or cyanobacteria [the photobiont]). Recent investigations evidenced that other fungi, non-photosynthetic bacteria and microalgae co-inhabit within the lichen thalli, but their d...
Article
Full-text available
This study analyses the interactions among crustose and lichenicolous lichens growing on gypsum biocrusts. The selected community was composed of Acarospora nodulosa, Acarospora placodiiformis, Diploschistes diacapsis, Rhizocarpon malenconianum and Diplotomma rivas-martinezii. These species represent an optimal system for investigating the strategi...
Article
Abstract Lichens provide valuable systems for studying symbiotic interactions. In lichens, these interactions are frequently described in terms of availability, selectivity and specificity of the mycobionts and photobionts towards one another. The lichen-forming, green algal genus Trebouxia Puymaly is among the most widespread photobiont, associati...
Article
Full-text available
Lichens undergo desiccation/rehydration cycles and are permeable to heavy metals, which induce free radicals. Nitrogen monoxide (NO) regulates important cellular functions, but the research on lichen NO is still very scarce. In Ramalina farinacea thalli, NO seems to be involved in the peroxidative damage caused by air pollution, antioxidant defence...
Article
Lichens provide valuable systems for studying symbiotic interactions. In lichens, these interactions are frequently described in terms of availability, selectivity and specificity of the mycobionts and photobionts towards one another. The lichen-forming, green algal genus Trebouxia Puymaly is among the most widespread photobiont, associating with a...
Article
The Trebouxiophyceae is the class of Chlorophyta algae from which the highest number of chloroplast genome (cpDNA) sequences have been obtained. Several species in this class participate in symbioses with fungi to form lichens. However, no cpDNA has been obtained from any Trebouxia lichen‐symbiont microalgae, which are present in approximately half...
Chapter
Full-text available
Lichens are symbiotic organisms, originated by mutualistic associations of heterotrophic fungi (mycobiont), photosynthetic partners (photobionts) which can be either cyanobacteria (cyanobionts) or green microalgae (phycobionts), and bacterial consortia. They are poikilohydric organisms without cuticles or nutrient absorption organs adapted to anhyd...
Article
Full-text available
Lichens are poikilohydrous symbiotic associations between a fungus, photosynthetic partners, and bacteria. They are tolerant to repeated desiccation/rehydration cycles and adapted to anhydrobiosis. Nitric oxide (NO) is a keystone for stress tolerance of lichens; during lichen rehydration, NO limits free radicals and lipid peroxidation but no data o...
Article
Full-text available
Trebouxiophyceae (Chlorophyta) is a species-rich class of green algae with a remarkable morphological and ecological diversity. Currently, there are a few completely sequenced mitochondrial genomes (mtDNA) from diverse Trebouxiophyceae but none from lichen symbionts. Here, we report the mitochondrial genome sequence of Trebouxia sp. TR9 as the firs...
Conference Paper
Full-text available
Lichens are complex symbiotic systems, in which coexistence occurs between several microalgal taxa and/or lineages with a single fungus-mycobiont-, displaying different tolerance patterns to multiple kinds of abiotic stress. Furthermore, certain communities of non-photosynthetic bacteria and yeasts are starting to be considered as an integral part...
Conference Paper
Full-text available
A taxonomical system for Trebouxia microalgae based on ultrastructural studies of Buellia zoharyi Galun and its subsequent statistical analysis is proposed in this study. Four different Trebouxia morphotypes were found in different areas of this lichen species. We needed to establish a taxonomical system allowing us to precisely classify these mor...
Conference Paper
Full-text available
Tolerance to saline conditions has been thoroughly studied in plants and, to an extent, freeliving microalgae. However, scientific data regarding salt stress on symbiotic lichen microalgae is scarce to non-existent. Since lichen phycobionts are capable of enduring harsh, restrictive and rapidly changing environments, it is interesting to study the...
Article
Full-text available
Main conclusion: For the first time we provide a study on the physiological, ultrastructural and molecular effects of salt stress on a terrestrial symbiotic green microalga, Trebouxia sp. TR9. Although tolerance to saline conditions has been thoroughly studied in plants and, to an extent, free-living microalgae, scientific data regarding salt stre...
Research Proposal
Full-text available
This meeting is dedicated to discussing and better clarifying our knowledge of the complex interactions that uphold lichen symbiosis, through the characterization of different aspects of biology and phylogenetic relationships within the symbionts. Outstanding international attendees experienced in different research field of lichen symbiosis will p...
Article
Phycologia: 2018, Vol. 57, No. 5, pp. 503-524. www.phycologia.org https://doi.org/10.2216/17-134.1 The class Trebouxiophyceae is comprised of coccoid to ellipsoid unicells, filaments, blades and colony-forming species of green algae occurring in diverse terrestrial and aquatic environments. Some representatives have evolved parasitic heterotrophic...
Article
Full-text available
https://onlinelibrary.wiley.com/doi/abs/10.1111/jpy.12751. https://rdcu.be/0FTE. Three vagrant (Circinaria hispida, Circinaria gyrosa, Circinaria sp. ‘paramerae’) and one crustose (semi‐vagrant, Circinaria sp.’oromediterranea’) lichens growing in very continental areas in the Iberian Peninsula were selected to study the phycobiont diversity. Myc...
Article
Full-text available
Intrathalline phycobiont diversity was investigated in a rosette-forming lichen, Parmotrema pseudotinctorum , using a combination of Sanger sequencing, 454-pyrosequencing, conventional light and confocal microscopy, and transmission electron microscopy. A total of 39 thalli sampled in five Canary Island populations were investigated. Three novel li...
Article
Full-text available
Ana Crespo: a 70th birthday tribute - Volume 50 Issue 3 - Pradeep K. DIVAKAR, Eva BARRENO, Leopoldo SANCHO, H. Thorsten LUMBSCH
Article
Full-text available
Myrmecia israeliensis has been traditionally considered as a green coccoid free–living microalga. This microalga was previously suggested as the primary phycobiont in the lichens Placidium spp., Heteroplacidium spp., and Psora decipiens. However, due to the absence of ITS rDNA sequences (barcode information) published along with these investigation...
Article
Full-text available
Lichen thalli represent the most conspicuous examples of fungal-algal interactions. Studies that describe phycobiont diversity within entire thalli are based mainly on Sanger sequencing. In some lichen species, this technique could underestimate the intrathalline coexistence of multiple microalgae. In this study different multi-tool approaches were...
Poster
Full-text available
Myrmecia israelensis (S.Chantanachat & H.Bold) T.Friedl has been traditionally considered as a green coccoid free-living microalga. This microalga was suggested as the primary phycobiont in two related lichen genera Placidium and Heteroplacidium, and recently in Psora decipiens. However, due to the absence of nrITS DNA sequences (barcode informatio...
Conference Paper
Full-text available
Biological Soil Crusts (BSCs) are biologically modified soil surfaces composed of a combination of organisms which include lichens, bryophytes, liverworts, microalgae, cyanobacteria, and microfungi. BSCs are complex communities due to the many different organisms involved and functional aspects they provide. Buellia zoharyi Galun is a prevailing li...
Article
Full-text available
The current literature reveals that the intrathalline coexistence of multiple microalgal taxa in lichens is more common thanpreviously thought, and additional complexity is supported by the coexistence of bacteria and basidiomycete yeasts in lichen thalli. This replaces the old paradigm that lichen symbiosis occurs between a fungus and a single pho...
Article
Desiccation tolerant (DT) organisms are able to withstand an extended loss of body water and rapidly resume metabolism upon rehydration. This ability, however, is strongly dependent on a slow dehydration rate. Fast dehydration affects membrane integrity leading to intracellular solute leakage upon rehydration and thereby impairs metabolism recovery...
Article
The identification of new organisms for environmental toxicology bioassays is currently a priority, since these tools are strongly limited by the ecological relevance of taxa used to study global change. Lichens are sensitive bioindicators of air quality and their microalgae are an untapped source for new low-cost miniaturized bioassays with ecolog...
Conference Paper
Full-text available
5eirophora vi/losa is an endangered lichen species, which grows on the twigs of shrubs and small trees in Mediterranean coastal dune ecosystems. This lichen is sensitive to nitrogen deposition and habitat fragmentation, and it is red­listed in ltaly. Fourteen populations of 5. vi/losa from Spain and ltaly were analysed. The mycobiont was identified...
Article
Full-text available
Lichens, self-supporting mutualistic associations between a fungal partner and one or more photosynthetic partners, also harbor non-photosynthetic bacteria. The diversity and contribution of these bacteria to the functioning of lichen symbiosis have recently begun to be studied, often by culture-independent techniques due to difficulties in their i...
Chapter
Full-text available
Symbiosis is a close and often long-term interaction between two different biological organisms, i.e. plants or fungi and microorganisms. Two main types of plant–microorganism interactions, mutualistic and cooperative, have been categorized. Mutualistic interactions, including nitrogen-fixing and mycorrhizal symbioses, refer to mostly obligate rela...
Data
Boxplot representation of lichen enriched media effect on the number of colony types of P. furfuracea associated bacteria from bulk thalli samples. Data are from two different samplings (a,b and c,d) with three replicates each. Data shows colony numbers of ectolichenic bacteria from washed thallus (a,c) and endolichenic bacteria from thallus tissue...
Conference Paper
Full-text available
Buellia zoharyi Galun is a widespread lichen on sun-exposed biological soil crusts (BSC) under xericMediterranean bioclimatic conditions. This species was said to be highly specific to gypsum soils, but it has also been found occasionally in a great variety of basic substrata. We have selected 24 different populations covering the entire range of t...
Article
http://pcp.oxfordjournals.org/cgi/content/abstract/pcw111? ijkey=CORtz9TU53IsjXv&keytype=ref Classically, lichen phycobionts are described as poikilohydric organisms able to undergo desiccation due to the constitutive presence of molecular protection mechanisms. However, little is known about the induction of cellular responses in lichen phycobion...
Chapter
Full-text available
Lichen Morphology Lichens originate from symbiosis, involving a fungus ‘mycobiont’ (the dominant partner) and one or several photosynthetic ‘photobionts’ (the energy producers), either unicellular green algae, cyanobacteria (see page 35) or both. The symbiosis is mutualistic since the fungus benefits from the food (carbohydrates) produced by algae...
Book
Full-text available
Key messages • Soil is an important habitat for thousand millions of organisms. • Soil biodiversity is extremely diverse in shapes, colours, sizes and functions. • Soil biodiversity is globally distributed, from deserts to polar regions through grasslands, forests, urban and agricultural areas. • Soil biodiversity supports many services essential t...
Article
Full-text available
"The final publication is available at http://link.springer.com/article/10.1007/s11120-015-0196-8 Lichens are poikilohydric symbiotic organisms that can survive in the absence of water. Photosynthesis must be highly regulated in these organisms, which live under continuous desiccation-rehydration cycles, to avoid photooxidative damage. Analysis of...
Article
The precise boundary delineations between taxa in symbiotic associations are very important for evolutionary and ecophysiological studies. Growing evidence indicates that in many cases, the use of either morphological characters or molecular markers results in diversity underestimation. In lichen symbioses, Trebouxia is the most common genus of lic...
Book
Symbiosis is a close and often long-term interaction between two different biological organisms, i.e. plants or fungi and microorganisms. Two main types of plant–microorganism interactions, mutualistic and cooperative, have been categorized. Mutualistic interactions, including nitrogen-fixing and mycorrhizal symbioses, refer to mostly obligate rela...
Book
Full-text available
SPE EA Pôle BIOME The Atlas is divided in 8 chapters covering all the aspects of soil biodiversity: - Chapter I: The soil habitat - Chapter II: Diversity of soil organisms - Chapter III: Geographical and temporal distribution - Chapter IV: Ecosystem functions and services - Chapter V: Threats - Chapter VI: Interventions - Chapter VII: Policy, educa...
Thesis
Full-text available
Lichens are complex symbiotic entities originated by cyclical associations and positive interactions of very different organisms, a heterotrophic fungus and one or several photosynthetic autotrophs "photobionts", either unicellular green algae, cyanobacteria or both. Also, specific bacterial communities remain obligate lichen symbionts. In the lich...
Conference Paper
Full-text available
Lichens are usually described as mutualistic and cyclical symbioses between a fungus and photosynthetic microorganisms, termed phycobionts (green microalgae or/and cyanobacteria). At present, lichen thalli are considered to be an example of micro-­‐ ecosystems due to the coexistence of several phycobionts inside a single thallus, as well as the pro...
Conference Paper
Lichens are usually described as mutualistic and cyclical symbioses between a fungus and photosynthetic microorganisms, termed phycobionts (green microalgae or/and cyanobacteria). At present, lichen thalli are considered to be an example of micro-­‐ ecosystems due to the coexistence of several phycobionts inside a single thallus, as well as the pro...
Conference Paper
Full-text available
Biological soil crusts (BSC) frequently cover open spaces in xeric territories and are set up by highly specialized communities of lichens, mosses, bacteria and fungi. Lichens are common components of these communities, adapted to extreme environmental conditions. Scientists are reviewing lichen characterization; these organisms are more complex th...