Eva Arrebola

Eva Arrebola
Spanish National Research Council | CSIC · Experimental Station "La Mayora"

About

76
Publications
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Introduction
Eva Arrebola currently works at the University of Malaga (Spain). Eva does research in Agricultural Plant Science, Genetics and Microbiology. Their most recent publication is 'Supplementary File 2.'

Publications

Publications (76)
Article
Full-text available
Aims Pseudomonas chlororaphis PCL1606 (PcPCL1606), displays strong antagonistic and biological control abilities against several soil-borne fungal pathogens mainly due to the production of the antifungal molecule 2-hexyl, 5-propyl resorcinol (HPR). HPR governs other beneficial phenotypes, suggesting its additional regulatory activity. Published tra...
Article
Full-text available
Pseudomonas chlororaphis (Pc) representatives are found as part of the rhizosphere-associated microbiome, and different rhizospheric Pc strains frequently perform beneficial activities for the plant. In this study we described the interactions between the rhizospheric Pc strains PCL1601, PCL1606 and PCL1607 with a focus on their effects on root per...
Article
Full-text available
The biocontrol rhizobacterium Pseudomonas chlororaphis is one of the bacterial species of the P. fluorescens group where insecticide fit genes have been found. Fit toxin, supported with other antimicrobial compounds, gives the bacterial the ability to repel and to fight against eukaryotic organisms, such as nematodes and insect larvae, thus protect...
Preprint
Full-text available
The biocontrol rhizobacterium Pseudomonas chlororaphis is one of the bacterial species of the P. fluorescens group where insecticide fit genes have been found. Fit toxin, supported with other antimicrobial compounds, gives the bacterial capacity to repel and fight against predators, such as nematodes and insect larvae, thus protecting the plant hos...
Article
Full-text available
Pseudomonas chlororaphis PCL1606 (PcPCL1606) is a rhizobacterium isolated from avocado roots, which is a favorable niche for its development. This strain extensively interacts with plant roots and surrounding microbes and is considered a biocontrol rhizobacterium. Genome sequencing has shown the presence of thirty-one potential methyl-accepting che...
Article
Full-text available
Flagellum mediated motility is an essential trait for rhizosphere colonization by pseudomonads. Flagella synthesis is a complex and energetically expensive process that is tightly regulated. In Pseudomonas fluorescens, the regulatory cascade starts with the master regulatory protein FleQ that is in turn regulated by environmental signals through th...
Article
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The goal of this mini review is to summarize the relevant contribution of some beneficial traits to the behavior of the species Pseudomonas chlororaphis, and using that information, to give a practical point of view using the model biocontrol strain P. chlororaphis PCL1606 (PcPCL1606). Among the group of plant-beneficial rhizobacteria, P. chlororap...
Article
Full-text available
The production of the compound 2-hexyl-5-propyl resorcinol (HPR) by the biocontrol rhizobacterium Pseudomonas chlororaphis PCL1606 (PcPCL1606) is crucial for fungal antagonism and biocontrol activity that protects plants against the phytopathogenic fungus Rosellinia necatrix. The production of HPR is also involved in avocado root colonization durin...
Article
Full-text available
The transcriptional regulator AmrZ is a global regulatory protein conserved within the pseudomonads. AmrZ can act both as a positive and a negative regulator of gene expression, controlling many genes implicated in environmental adaption. Regulated traits include motility, iron homeostasis, exopolysaccharides production and the ability to form biof...
Article
Full-text available
The Pseudomonas fluorescens complex of species includes plant-associated bacteria with potential biotechnological applications in agriculture and environmental protection. Many of these bacteria can promote plant growth by different means, including modification of plant hormonal balance and biocontrol. The P. fluorescens group is currently divided...
Data
GGDC intergenomic distances of 421 Pseudomonas genomes.
Data
Closest-related strain and phylogroup adscription according to 16S sequence identity.
Article
Full-text available
The genome sequence of more than 100 Pseudomonas syringae strains has been sequenced to date; however only few of them have been fully assembled, including P. syringae pv. syringae B728a. Different strains of pv. syringae cause different diseases and have different host specificities; so, UMAF0158 is a P. syringae pv. syringae strain related to B72...
Article
Full-text available
Mango malformation disease (MMD) has become an important global disease affecting this crop. The aim of this study was to identify the main causal agents of MMD in the Axarquia region of southern Spain and determine their genetic diversity. Fusarium nzangiferae was previously described in the Axarquia region but it represented only one-third of the...
Article
Full-text available
Genome sequencing and annotation have revealed a putative cellulose biosynthetic operon in the strain Pseudomonas syringae pv. syringae UMAF0158, the causal agent of bacterial apical necrosis. Bioinformatics analyses and experimental methods were used to confirm the functionality of the cellulose biosynthetic operon. In addition, the results showed...
Article
Full-text available
Bacillus amyloliquefaciens strains CECT 8237 and CECT 8238, formerly known as Bacillus subtilis UMAF6639 and UMAF6614, respectively, contribute to plant health by facing microbial pathogens or inducing the plant's defence mechanisms. We sequenced their genomes and developed a set of ad hoc scripts that allowed us to search for the features implicat...
Article
Full-text available
During the last years, Fusarium strains have been isolated from shoots and inflorescences of mango trees affected with floral and vegetative malformation in different orchards from the Axarquia region (south of Spain), highlighting the identification of Fusarium mangiferae. With the aim of elucidate epidemiological aspects and design more efficient...
Article
Full-text available
The antimetabolite mangotoxin is a key factor in virulence of Pseudomonas syringae pv. syringae strains which cause apical necrosis of mango trees. Previous studies showed that mangotoxin biosynthesis is governed by the mbo operon. Random mutagenesis led to the identification of two other gene clusters that affect mangotoxin biosynthesis. These are...
Article
Full-text available
During the last years, Fusarium strains have been isolated from shoots and inflorescences of mango trees affected with floral and vegetative malformation in different orchards from the Axarquía region (south of Spain), highlighting the identification of Fusarium mangiferae. With the aim of elucidate epidemiological aspects and design more efficient...
Article
Full-text available
ABSTRACT Pseudomonas syringae pv. syringae, the causal agent of bacterial apical necrosis (BAN) in mango crops, has been isolated in different mango-producing areas worldwide. An extensive collection of 87 P. syringae pv. syringae strains isolated from mango trees affected by BAN from different countries, but mainly from Southern Spain, were initia...
Chapter
Full-text available
The biocontrol rhizobacterium Pseudomonas fluorescens PCL1606 has strong antagonistic activity against many soil-borne phytopathogenic fungi, including Rosellinia necatrix, the causal agent of white root rots of many plants and Fusarium oxysporum f. sp. radicis-lycopersici, which causes the tomato foot and root rot The relevant characteristic of th...
Article
Full-text available
Mangotoxin production was first described in Pseudomonas syringae pv. syringae strains. A phenotypic characterization of 94 P. syringae strains was carried out to determine the genetic evolution of the mangotoxin biosynthetic operon (mbo). We designed a PCR primer pair specific for the mbo operon to examine its distribution within the P. syringae c...
Article
Bacterial apical necrosis (BAN), caused by the bacterium Pseudomonas syringae pv. syringae (Pss), is currently the most limiting disease affecting the mango crop in the Mediterranean area. The copper‐based compound Bordeaux mixture (BM) is considered to be the conventional treatment against BAN, but it does not act as a bactericide. Alternative exp...
Article
Full-text available
Mangotoxin is an antimetabolite toxin produced by certain Pseudomonas syringae pv. syringae strains. This toxin is an oligopeptide that inhibits ornithine N-acetyl transferase, a key enzyme in the biosynthesis of ornithine and arginine. Previous studies have reported the involvement of the putative nonribosomal peptide synthetase MgoA in virulence...
Data
Pairwise alignments between the genome of P. syringae pv. syringae B728a and pCG1-5 from P. syringae pv. syringae UMAF0158 (Psy B728a and Psy UMAF0158 pCG1-5). Axes represent the genes in the order in which they occur on the chromosomes. Top axis, pCG1-5; bottom axis, B728a. The co-linear regions of similarity on both genomes are represented in red...
Data
Polarity determination of insertional mutants by RT-PCR experiments. RT-PCRs of the internal and intergenic regions were performed with RNA obtained from different insertional P. syringae pv. syringae UMAF0158 mutants in each gene of mbo operon. The primer pairs used for each reaction are detailed in Table S2 and schematic representation of the amp...
Data
Comparison of the PmboI sequence motif between different P. syringae strains. This alignment was analysed using Jalview software. A summary of the tendency of each nucleotide to hold each position is represented under the alignment as a consensus sequence. The predicted -10 (position 21), -35 (position 42) boxes and crp box are marked in solid line...
Data
Primers used in mbo genes mutation experiments, amplicons containing an internal fragment of each gene, were cloned in pCR2.1 for mutagenesis by integration. (DOC)
Data
Construction of the pLac-AF and pLac-FA vector derivatives from pBBR1MCS-5. The complete mbo operon, including the regulatory sequences (putative promoter and terminator), was cloned into both vectors. In pLac-AF, the mbo operon is under the control of the PLAC promoter with constitutive expression in Pseudomonas spp. and the own promoter of the mb...
Data
Primers used in RT-PCR experiments. NC1 and NC2 correspond to non-coding adjacent region upstream to mboA gene. (DOC)
Article
Full-text available
Mangotoxin is an antimetabolite toxin that is produced by strains of Pseudomonas syringae pv. syringae; mangotoxin-producing strains are primarily isolated from mango tissues with symptoms of bacterial apical necrosis. The toxin is an oligopeptide that inhibits ornithine N-acetyl transferase (OAT), a key enzyme in the biosynthetic pathway of the es...
Data
Figure S1. Analysis of the plasmid integration in UMAF0158::mgoB. The PCR was performed using the M13F primer located in the lacZ gene of the pCR2.1 cloning vector and the ORF4204R primer located in the 5'-end of mgoC. Lane L: HyperLadder I (Bioline), lane 2: UMAF0158::mgoB, lane 3: UMAF0158, lane 4: negative control of the PCR reaction.
Data
Full-text available
Table S1. The annealing position and the sequence of the utilized primers in RT-PCR experiments.
Article
Full-text available
La malformacion del mango es una de las enfermedads más importantes que afectan a este cultivo en todo el mundo, causando imporantes perdidas economicas. Este trabajo pone de manifiesto de manera concluyente la presencia por vez primera de la malformación del mango en España y la determinacion de su agente causal
Chapter
Pseudomonas fluorescens PCL1606 is a rhizobacteria with biocontrol activity against many soil-borne phytopathogenic fungi. Its antagonist activity strongly correlates with the production of the antibiotic 2-hexyl, 5-propyl resorcinol (HPR). However, other antibiotics could be detected in P. fluorescens PCL1606, such as pyrrolnitrin (PRN) and hydrog...
Article
Full-text available
Pseudomonas syringae is pathogenic in a wide variety of plants, causing diseases with economic impacts. Pseudomonas syringae pathovars produce several toxins that can function as virulence factors and contribute to disease symptoms. These virulence factors include antimetabolite toxins, such as tabtoxin, phaseolotoxin and mangotoxin, which target e...
Article
Full-text available
Pseudomonas syringae is a phytopathogenic bacterium present in a wide variety of host plants where it causes diseases with economic impact. The symptoms produced by Pseudomonas syringae include chlorosis and necrosis of plant tissues, which are caused, in part, by antimetabolite toxins. This category of toxins, which includes tabtoxin, phaseolotoxi...
Article
Full-text available
El mango (Mangifera indica L.) es un cultivo subtropical de origen asiático que se encuentra distribuido en zonas tropicales y subtropicales de todo el mundo. En el área mediterránea podemos encontrar este cultivo en Israel, Egipto, Italia y en el sur de la península ibérica, principalmente en Málaga y Granada. En la zona oriental de la provincia d...
Conference Paper
Pseudomonas syringae pv. syringae (Pss) is an opportunist pathogen of a wide variety of plants where it lives as epiphytic microorganism most of the time. A 62-kb indigenous plasmids has been found in nearly all Pss isolated from mango as the most generalized one. It contains genes homologous to those present in the copABCD and rulAB operon, both r...
Article
Bacillus amyloliquefaciens PPCB004 was selected as a potential antagonist to control Botrytis cinerea, Penicillium expansum and Rhizopus stolonifer on peach fruit. The HPLC data of PPCB004 indicated the lipopeptides iturin A, fengycin and surfactin as secondary metabolites. The GC/MS analysis of PPCB004 showed 3-hydroxy-2-butanone as the dominant c...
Article
The antifungal effects of volatile compounds produced by Bacillus strains; Bacillus subtilis PPCB001 or Bacillus amyloliquefaciens PPCB004 and antagonist combination (PPCB001+PPCB004) against Penicillium digitatum Sacc., Penicillium italicum Wehmer and Penicillium crustosum Thom isolates were investigated in vitro and in vivo. The antagonists alone...
Article
Full-text available
Greening disease of citrus is a serious disease known in South Africa since the late 1920s. In South Africa, it is associated with infection by 'Candidatus Liberibacter africanus', a heat sensitive, phloem-limited, noncultured alpha-proteobacterium. Huanglongbing (HLB), a similar, but more devastating disease that was described initially from China...
Article
A Bacillus amyloliquefaciens strain, surviving epiphytically on the surface of fruit, was isolated while searching for naturally occurring biological control agents. This bacterial strain was characterized for its antifungal activity against seven selected fungal postharvest pathogens of citrus. To understand the antifungal activity, seven postharv...
Article
Full-text available
Mangotoxin is an antimetabolite toxin that inhibits ornithine acetyl transferase, a key enzyme in the biosynthetic pathway of ornithine and arginine and recently reported in strains of Pseudomonas syringae pv. syringae (Pss) isolated from mango. Since symptoms on mango tissues are very difficult to reproduce, in this study the role of mangotoxin in...
Article
Full-text available
The presence of genetic determinants homologous to rulAB genes for ultraviolet (UV) radiation resistance was determined in a collection of Pseudomonas syringae pv. syringae strains isolated from mango. The potential role of these plasmids in UV tolerance and ecological fitness in the mango phyllosphere was also evaluated. Nearly all of the 62-kb pl...
Article
The effects of modified atmosphere packaging (MAP) [bioriented polypropylene (BOPP-1 or BOPP-2)] in combination with antimicrobial agents Bacillus subtilis, 107 colony-forming units (cfu) ml−1; ethylenediamine tetraacetic acid, calcium disodium salt hydrate (EDTA) (0.1%); or 4-hexylresorcinol (4-HR) (0.15%) on postharvest decay control and quality...
Chapter
Full-text available
Bacterial apical necrosis of mango, produced by Pseudomonas syringae pv. syringae (Pss), is the main disease affecting mango production in the Mediterranean area. Surveys carried out in the main areas of cultivation ascertained the presence of Pss strains and resulted in a collection of Pss strains from different seasons and locations (including ma...
Article
Full-text available
Pseudomonas syringae pv. syringae, which causes the bacterial apical necrosis of mango, produces the antimetabolite mangotoxin. We report here the cloning, sequencing, and identity analysis of a chromosomal region of 11.1 kb from strain P syringae pv. syringae UMAF0158, which is involved in mangotoxin biosynthesis. This chromosomal region contains...
Article
Full-text available
Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain. Four Bacillus subtilis strains, UMAF6614, UMAF6619, UMAF6639, and UMAF8561, with proven ability to suppress the disease on melon in detached leaf and seedling assays, were subjected to further analyses to elucidate the mode of action involved in their biocontrol perform...
Article
Full-text available
Bacterial apical necrosis is a critical disease in the main production area of mango in Europe. It is caused by Pseudomonas syringae pv. syringae, and produces necrotic lesions on mango buds and leaves, causing severe yield losses due to a decrease of flowering and fruit set. A field study to evaluate control treatments against bacterial apical nec...
Conference Paper
Full-text available
La mayoría de las cepas de Pseudomonas syringae pv. syringae (Pss) aisladas de mango producen mangotoxina, que tiene como diana a la enzima Ornitina acetil-transferasa (OAT) y actúa como antimetabolito en la ruta de biosíntesis de ornitina y arginina. A partir de la cepa silvestre Pss UMAF0158 productora de mangotoxina, se han construido y seleccio...
Article
Bacterial leaf spot symptoms of coriander were first observed in January 2003 in three coriander fields in the valley region of the Axarquía (Málaga, Spain), showing a very high incidence. Pseudomonas syringae pv. coriandricola was consistently isolated from diseased plants, identified and its pathogenicity on coriander could be proved. The effecti...
Article
Pseudomonas syringae pv. syringae is a common inhabitant of a large variety of plants and the causal agent of bacterial apical necrosis of mango. In this work, we describe production and preliminary characterization of a novel antimetabolite toxin, designated mangotoxin, by P. syringae pv. syringae strains mainly isolated from mango trees. The toxi...
Chapter
In this study, we reported the production of an antimetabolite toxin (named mangotoxin), mainly by strains of Pseudomonas syringae pv. syringae pathogenic to mango. The target of mangotoxin is the enzymatic step where ornithine is produced from N-acetyl ornithine in the biosynthetic pathway of arginine