Eugene Manley, Jr.

LUNGevity · Research

We investigate the hypoxia-dependent cytotoxicity of AQ4N (banoxantrone) using a panel of 13 cancer cell lines and its relationship to the expression of the quinone reductase DT-diaphorase (NQO1), which is widely found in cancer cells. We also investigate pharmacological treatments that increase tumor hypoxia in vivo and their impact on AQ4N chemos...

Thombospondin-2 (TSP2) is a matricellular protein component of the bone extracellular matrix (ECM). Long bones of adult TSP2-deficient mice have increased endosteal bone thickness due to expansion of the osteoblast progenitor cell pool, and these cells display deficits in osteoblastic potential. Here, we investigated the effects of TSP2-deficiency...

Thrombospondin-1 and 2 have each been implicated in collagen fibrillogenesis. We addressed the possibility that deficits in lysyl oxidase (LOX) contribute to the extracellular matrix (ECM) phenotype of TSP-deficient bone. We examined detergent insoluble (mature cross-linked) and soluble (newly secreted) ECM fractions prepared from diaphyseal cortic...

This poster describes the criteria for development of antibodies for cyclic multiplex immunofluorescence in mouse tissues. 5 um sections of FFPE mouse (C57/B6) lung, liver, spleen, and mammary fat pad (MFP) in the same block were subjected to antigen retrieval. Three different slides were stained with 5 rounds of antibodies labelled with Alexa Fluo...

Purpose: We created a mentoring program to increase workforce diversity in thoracic oncology with the goal of decreasing racial disparities and improving outcomes, that was inspired by the latest American Society for Clinical Oncology report which showed that the oncology workforce is not diverse or representative of the cancer patient population....

The fifth annual summer research summit organized by the Center of Excellence (COE) in Health Equity, Training and Research, Baylor College of Medicine (BCM), was held on May 17, 2022. The theme of this year’s summit was ‘Academic-Community Partnerships: Change Agents for Advancing Health Equity.’ Given the ongoing pandemic, the summit was conducte...

Introduction To support the care of lung cancer patients, oncologists have needed to stay current on treatment advancements and build relationships with a new group of survivors in an era where lung cancer survivorship has been re-defined. The objectives of the study were to (1) understand the perspectives of advanced lung cancer patients whose tum...

Introduction: To support the care of lung cancer patients, oncologists have needed to stay current on treatment advancements and build relationships with a new group of survivors in an era where lung cancer survivorship has been re-defined. The objectives of the study were to: 1) understand the perspectives of advanced lung cancer patients whose tu...

Introduction/Background: Cyclic multiplex immunofluorescence (cmIF) is a novel technique that allows molecular imaging and quantitative analysis of normal and diseased tissues at single-cell resolution. cmIF surpasses IHC since tissues can be re-stained, spatial information retained, and commercially available antibodies are used. We hypothesize cm...

DISCLOSURES: The authors have nothing to disclose. INTRODUCTION: Genes that alter bone strength and thus contribute to mechanical homeostasis have previously been identified by others when selectively perturbing the Wnt pathway. However, it remains unclear how genes and their interactions within the Wnt pathway contribute to the natural variation i...

Targeted perturbations within the Wnt pathway identified genes that altered bone strength and thus contribute to mechanical homeostasis. However, it remains unclear how genes and their interactions within the Wnt pathway contribute to normal bone mechanical function. We tested the hypothesis that two inbred mouse strains (A/J, C57BL/6J (B6)) that a...

Thrombospondin-2-deficiency is associated with impaired matrix maturation in osteoblasts and cortical bone of growing mice. Here we addressed the possibility that lysyl oxidase (LOX) contributes to this phenotype. After overnight serum starvation, pro-LOX levels were elevated compared to wild-type in marrow-derived osteoblasts from male and female...

Previously, we showed that cortical mineralization is coordinately adjusted to mechanically offset external bone size differences between A/J (narrow) and C57BL/6J (wide) mouse femora to achieve whole bone strength equivalence at adulthood. The identity of the genes and their interactions that are responsible for establishing this homeostatic state...

Cancer stem-like cells were isolated from several human tumor cell lines by limiting dilution assays and holoclone morphology, followed by assessment of self-renewal capacity, tumor growth, vascularity, and blood perfusion.H460 holoclone-derived tumors grew slower than parental H460 tumors, but displayed significantly increased microvessel density...

Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC The bioreductive drug AQ4N is reduced enzymatically under hypoxic conditions to yield AQ4, a cytotoxic metabolite and topoisomerase II inhibitor. This study tests the hypothesis that the two electron reductase DT-diaphorase (NQO1) contributes to AQ4N reduction and that m...

The goal of this study was to determine the duration of time that ligaments from a study group need to be loaded in order to adequately determine their collective viscoelastic behavior. Rat ligaments were subjected either to creep or stress relaxation for 1,000 s or stress relaxation for 10,000 s to compare estimates of viscoelastic behavior for di...