Esther Kanduma

Esther Kanduma
University of Nairobi | UON · Department of Biochemistry

PhD Biochemistry
Molecular diagnostics, surveillance, genetic diversity and control of tick vectors and tick-borne pathogens

About

126
Publications
10,101
Reads
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905
Citations
Citations since 2016
51 Research Items
591 Citations
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
Introduction
A biochemist with special research interest in genetic diversity studies of tick vectors and tick-borne parasites, molecular diagnostics and vaccines for tick-borne diseases. My current research goal is to advance my scientific and research knowledge and skills in evidence-based research for policy development to improve animal productivity and livelihoods of small-holder farmers. https://orcid.org/0000-0002-4979-2681
Additional affiliations
July 2012 - May 2020
University of Nairobi
Position
  • Lecturer
Description
  • Teaching Biochemistry, Molecular Biology and Parasitology. Involved in research in the field of ticks and tick-borne diseases in areas of population genetics and molecular diversity. Passionate about science mentorship
October 2008 - September 2014
Biosciences eastern and central Africa (BecA) Hub
Biosciences eastern and central Africa (BecA) Hub
Position
  • Research/Graduate Fellow
Description
  • Undertook a PhD research project on genetic diversity of Rhipicephalus appendiculatus, a tick vector. Carried out a post-doc project on development of a rapid diagnostic kit for detection of Theileria parva in cattle
June 2004 - present
University of Nairobi
Position
  • Lecturer
Description
  • Teaching basic and advanced general and medical Biochemistry, molecular biology and research skills to both undergraduate and postgraduate students, conducting practicals and tutorials.
Education
September 1992 - December 2014
University of Nairobi
Field of study
  • Biochemistry

Publications

Publications (126)
Article
Full-text available
The mitochondrial genome (mitogenome) has proven to be important for the taxonomy, systematics, and population genetics of ticks. However, current methods to generate mitogenomes can be cost-prohibitive at scale. To address this issue, we developed a cost-effective approach to amplify and sequence the whole mitogenome of individual tick specimens....
Article
Full-text available
Background Among protozoan parasites in the genus Babesia , Babesia bigemina is endemic and widespread in the East African region while the status of the more pathogenic Babesia bovis remains unclear despite the presence of the tick vector, Rhipicephalus microplus , which transmits both species. Recent studies have confirmed the occurrence of R. mi...
Article
Full-text available
In many African countries, tick control has recently been the responsibility of resource-poor farmers rather than central government veterinary departments. This has led to an increase in acaricide resistance, threatening the welfare of livestock farmers in sub-Saharan Africa. Resistance has evolved to the three classes of acaricides used most exte...
Article
Full-text available
Data on the epidemiology of Entamoebainfections in Eastern part of Kenya is scanty. Diagnostic tests in use have limited capacity to differentiate common infecting species. The performance ofPolymerase Chain Reaction (PCR) to differentiate between pathogenic Entamoeba histolyticaand two nonpathogenic species E. dispar and E. moshkovskii is largely...
Article
Full-text available
Data on the epidemiology of Entamoeba infections in Eastern part of Kenya is scanty. Diagnostic tests in use have limited capacity to differentiate common infecting species. The performance of Polymerase Chain Reaction (PCR) to differentiate between pathogenic Entamoeba histolytica and two nonpathogenic species E. dispar and E. moshkovskii is large...
Article
Full-text available
A recent research study on prevalence of tick-borne pathogens in Burundi reported high prevalence and endemicity of Theileria parva , Anaplasma marginale and Babesia bigemina infections in cattle. Detailed information about tick species infesting animals, their distribution and genetic diversity in Burundi is outdated and limited. This study theref...
Chapter
Full-text available
This book is a collection of 77 expert opinions arranged in three sections. Section 1 on "Climate" sets the scene, including predictions of future climate change, how climate change affects ecosystems, and how to model projections of the spatial distribution of ticks and tick-borne infections under different climate change scenarios. Section 2 on "...
Preprint
Full-text available
Background Among protozoan parasites in the genus Babesia, Babesia bigemina is endemic and widespread in the East African region while the status of the more pathogenic Babesia bovis remains unclear despite the presence of the tick vector, Rhipicephalus microplus which transmits both species. Recent studies have confirmed the occurrence of R. micro...
Article
Full-text available
Ticks and tick-borne pathogens (TBPs) are major constraints to camel health and production, yet epidemiological data on their diversity and impact on dromedary camels remain limited. We surveyed the diversity of ticks and TBPs associated with camels and co-grazing sheep at 12 sites in Marsabit County, northern Kenya. We screened blood and ticks (85...
Preprint
Full-text available
Ticks and tick-borne pathogens (TBPs) are major constraints to camel health and production , yet epidemiological data on their diversity and impact on dromedary camels are limited. We surveyed the diversity of ticks and TBPs associated with camels and co-grazing sheep at 12 sites in Marsabit County, northern Kenya. We screened blood and ticks (858...
Article
Full-text available
Background Tick-borne diseases (TBDs) constitute a major constraint for livestock development in sub-Saharan Africa, with East Coast fever (ECF) being the most devastating TBD of cattle. However, in Burundi, detailed information is lacking on the current prevalence of TBDs and on the associated economic losses from mortality and morbidity in cattle...
Article
Full-text available
Abstract Background: Anaplasma and Ehrlichia species are tick-borne pathogens of both veterinary and public health importance. The current status of these pathogens, including emerging species such as Ehrlichia minasensis and Anaplasma platys, infecting cattle in Kenya, remain unclear, mainly because of limitation in the diagnostic techniques. Ther...
Article
Full-text available
Background: Anaplasma and Ehrlichia species are tick-borne pathogens of both veterinary and public health importance. The current status of these pathogens, including emerging species such as Ehrlichia minasensis and Anaplasma platys, infecting cattle in Kenya, remain unclear, mainly because of limitation in the diagnostic techniques. Therefore, w...
Article
Full-text available
Background: The tick vector Rhipicephalus microplus which transmits Babesia spp. and rickettsial pathogens has not been reported in Kenya since 1998. More recently, the pathogenic Babesia bovis has been detected in cattle blood DNA. The status of R. microplus in Kenya remains unknown. This study employed morphological and molecular tools to charac...
Preprint
Full-text available
Background The tick vector Rhipicephalus (Boophilus) microplus which transmits Babesia and rickettsial pathogens has not been reported in Kenya since 1998. More recently, the pathogenic Babesia bovis has been detected in cattle blood DNA. The status of R. microplus in Kenya remains unknown. This study employed morphological and molecular tools to c...
Poster
Full-text available
Technical Report
Full-text available
Poster
Full-text available
Article
Full-text available
Theileria parva is a protozoan parasite transmitted by the brown ear tick Rhipicephalus appendiculatus that causes East Coast fever (ECF) in cattle, resulting in substantial economic losses in the regions of southern, eastern and central Africa. The schizont form of the parasite transforms the bovine host lymphocytes into actively proliferating can...
Data
Confirmation of indels in the transcripts by PCR and sequencing. (DOCX)
Data
Differentially expressed genes between the three stages studied. (XLSX)
Data
Differentially expressed genes of unmapped reads. (CSV)
Data
Average Transcripts Per Kilobase Million (TPM) of genes, genes hit by unmapped reads and, T. parva orthologs of apicomplexan genes encoding proteins of sporozoite invasion organelles. (XLSX)
Article
The systematics of the genera and subgenera within the soft tick family Argasidae is not adequately resolved. Different classification schemes, reflecting diverse schools of scientific thought that elevated or downgraded groups to genera or subgenera, have been proposed. In the most recent classification scheme, Argas and Ornithodoros are paraphyle...
Article
Full-text available
Background: The ixodid tick Rhipicephalus appendiculatus is the main vector of Theileria parva, wich causes the highly fatal cattle disease East Coast fever (ECF) in sub-Saharan Africa. Rhipicephalus appendiculatus populations differ in their ecology, diapause behaviour and vector competence. Thus, their expansion in new areas may change the geneti...
Article
Full-text available
Bacterial blight of cassava is one of the most important diseases of cassava worldwide due to its growing concern, widespread and destructive nature. Even though the use of resistant cultivars is the most effective management strategy for the disease, such cultivars are not well identified. Therefore, the objective of this study was to screen 21 ca...
Article
Full-text available
Background The ixodid tick Rhipicephalus appendiculatus transmits the apicomplexan protozoan parasite Theileria parva, which causes East coast fever (ECF), the most economically important cattle disease in eastern and southern Africa. Recent analysis of micro- and minisatellite markers showed an absence of geographical and host-associated genetic s...
Method
Full-text available
A summary of an experimental approach that can be used to screen for, identify, amplify, detect, validate and use microsatellite (SSR) markers for genetic diversity studies. Examples of methods that can be used for analysis of diversity are suggested
Article
Full-text available
Background The tick population of Africa includes several important genera belonging to the family Ixodidae. Many of these ticks are vectors of protozoan and rickettsial pathogens including Theileria parva that causes East Coast fever, a debilitating cattle disease endemic to eastern, central and southern Africa. Effective surveillance of tick-born...
Article
Full-text available
Rhipicephalus appendiculatus is an important tick vector of several pathogens and parasitizes domestic and wild animals across eastern and southern Africa. However, its inherent genetic variation and population structure is poorly understood. To investigate whether mammalian host species, geographic separation and resulting reproductive isolation,...
Article
Full-text available
Rhipicephalus appendiculatus is an important tick vector of several pathogens and parasitizes domestic and wild animals across eastern and southern Africa. However, its inherent genetic variation and population structure is poorly understood. To investigate whether mammalian host species, geographic separation and resulting reproductive isolation,...
Article
Full-text available
Theileria parva (T. parva) causes East Coast fever (ECF), which is of huge economic importance to Eastern and Southern African countries. In a previous bovine model, inflammatory cytokines were closely associated with disease progression in animals experimentally infected with T. parva. The African Cape buffalo (Syncerus caffer), the natural reserv...
Article
Full-text available
Piroplasms frequently infect domestic and wild carnivores. At present, there is limited information on the occurrence and molecular identity of these tick-borne parasites in wild felids in Kenya. In 2009, a pair of captive lions (Panthare leo) was diagnosed with suspected babesiosis and mineral deficiency at an animal orphanage on the outskirts of...
Article
Biological differences, including vector competence for the protozoan parasite Theileria parva have been reported among populations of Rhipicephalus appendiculatus (Acari: Ixodidae) from different geographic regions. However, the genetic diversity and population structure of this important tick vector remain unknown due to the absence of appropriat...