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Publications (59)
Bacteria belonging to the Burkholderia genus have been extensively studied for their potential as biocontrol agents, particularly their ability to produce bioactive compounds. In this study, we investigated the antagonistic capabilities of an indigenous strain of Burkholderia gladioli BNM349 against a wide spectrum of bacterial and fungal phytopath...
The Burkholderia species are prolific producers of bioactive secondary metabolites (antibacterial, antifungal, antitumoral, antinematodal, herbicidal and insecticidal compounds) with many potential biotechnological applications. Burkholderia gladioli was initially identified as a pathogen of flowering plants of genus Gladiolus and later isolated fr...
Burkholderia ambifaria T16 is a bacterium isolated from the rhizosphere of barley plants that showed a remarkable antifungal activity. This strain was also able to degrade fusaric acid (5-Butylpyridine-2-carboxylic acid) and detoxify this mycotoxin in inoculated barley seedlings. Genes and enzymes responsible for fusaric acid degradation have an im...
Las bacterias pertenecientes al género Burkholderia poseen una gran versatilidad metabólica y son capaces de sintetizar combinaciones únicas de moléculas bioactivas cepa-dependientes, algunas de las cuales presentan un alto potencial biotecnológico para su aplicación como biopesticidas. Nuestro laboratorio cuenta con las cepas nativas B. ambifaria...
Burkholderia ambifaria T16 is a bacterial strain isolated from the rhizosphere of barley plants, which showed a potent antifungal activity against several Fusarium species. In this work, the construction of a mini-Tn5 insertional library in this strain allowed us the identification of several genes involved in the antifungal activity. In particular...
Pseudomonas protegens synthesizes two major iron-chelating metabolites (siderophores): pyoverdine (Pvd) and enantio-pyochelin (E-Pch). Although iron sequestration and uptake seem to be the main biological role of these siderophores, other functions including metal homeostasis and antibiotic activity have been proposed. The aim of this study was to...
Las ciencias agrarias y ambientales desde diferentes enfoques y niveles de análisis
Burkholderia ambifaria T16 is a bacterial strain isolated from the rhizosphere of barley which showed the ability to inhibit mycelial growth and conidial germination of several phytopathogenic fungi, including different Fusarium spp. With the aim to identify the genes responsible for this potent antifungal activity, an insertional mini-Tn5 library...
We examined the biocontrol and the differential production of H2O2 and in the relationship Macrophomina phaseolina – PGPR in soybean seedling. Fungal colonisation was efficiently prevented by inoculation with Pseudomonas fluorescens 9. Its ability to improve ROS production and gene expression of antioxidant enzymes could be related to its capacity...
Abstract. Soybean root and stem rots caused by soil-borne pathogens are diseases commonly found in soybean fields, and one of the most important causes of crop losses. In the present study, the mycelial sensitivity of Fusarium virguliforme, F. tucumaniae, Sclerotin-ia sclerotiorum and Macrophomina phaseolina was evaluated on potato dextrose agar me...
Fusaric acid (FA) is a fungal metabolite produced by several Fusarium species responsible for wilts and root rot diseases of a great variety of plants. Bacillus spp. and Pseudomonas spp. have been considered as promising biocontrol agents against phytopathogenic Fusarium spp., however it has been demonstrated that FA negatively affects growth and p...
Results:
showed that subtilase specific activity as well as two inmunoreactive bands representing putative subtilases increased in barley leaves submitted to natural and dark-induced senescence. Gene expression analysis showed that two of the eleven subtilase genes analyzed, HvSBT3 and HvSBT6, were up-regulated in all the senescence conditions tes...
The aim of the present study was to evaluate indigenous PGPR (Plant growth promoting rhizobacteria) previously isolated from Argentina's soybean fields for their in vitro antagonistic effects on the control of Fusarium tucumaniae and F. virguliforme, in two separated in vitro assays. In assay 1, the bacteria that showed the highest significant (P <...
Plant growth promoting rhizobacteria (PGPR) are potential agents to control plant pathogens and their combined use with biopesticides such as phosphites may constitute a novel strategy to incorporate in disease management programs. In the present study, 11 bacterial isolates were selected on the basis of their antagonistic activity against Macropho...
Fusarium tucumaniae is the primary causal agent of sudden death syndrome (SDS) of soybean in Argentina. It is a soil-borne pathogen that causes root rot followed by the development of foliar symptoms, causing yield losses. Early infections, occurring at the seed stage, produced foliar symptoms and higher root rot severity, when compared to later in...
Assessment of biological control of Cercospora sojina, causal agent of frogeye leaf spot (FLS) of soya bean, using three indigenous bacterial strains, BNM297 (Pseudomonas fluorescens), BNM340 and BNM122 (Bacillus amyloliquefaciens).
From cultures of each bacterial strain, cell suspensions and cell-free supernatants were obtained and assayed to dete...
Strains of Pseudomonas fluorescens and Bacillus amyloliquefaciens, isolated from soybean rhizosphere, inhibited mycelial growth of Botrytis cinerea and Sclerotinia sclerotiorum, in vitro. Leaves from Brassica napus seedlings, pre-inoculated with either of these bacteria, exhibited systemic protection against fungal pathogens.
In a previous work, deduced amino acid sequences from twenty wheat peroxidase genes were assigned to seven groups designated as TaPrx108 to TaPrx114. Some of these apoplastic peroxidases have previously shown to play different roles in the plant defense responses to infection by the cereal cyst nematode Heterodera avenae. In the present study, PCR...
Changes in ascorbate peroxidase (APX) enzyme activity in response to nematode (Heterodera avenae) attack were studied in roots of three hexaploid wheat lines carrying Cre2, Cre5, or Cre7 nematode resistance genes and the susceptible Triticum aestivum cv. Anza. A spectrophotometric analysis was carried out with root extracts of infected plants 4, 7,...
The response of resistant wheat-Aegilops ventricosa introgression line H-93-8 and its susceptible parent, Triticum aestivum H-10-15, to Ha71 Spanish population of Heterodera avenae was studied to determine the changes in peroxidase gene expression during incompatible and compatible wheat-nematode interactions. Twenty peroxidase genes were character...
We have used in situ hybridization combining genomic and repeated DNA fluorescent probes to determine the karyotype composition of two bread wheat introgression lines: H-93-33, which carries the gene H27 for resistance to the Hessian fly M. destructor (Delibes et al. 1997); and H-93-8, carrying the gene Cre2 which confers resistance to the cereal c...
The incompatible interaction between plant and pathogen is often determined by the hypersensitive reaction (HR). This response is associated with accumulation of reactive oxygen species (ROS), which results in adverse growth conditions for pathogens. Two major mechanisms involving either NADPH oxidases or peroxidases have been proposed for generati...
Effects of introgressed 4Nv Aegilops ventricosa chromosome on yield and yield components in bread wheat
An intense exploration of the Spanish Cantabrian coast for the presence or absence of wild Brassica oleracea L., yielded 24 new localities to be added to the 21 previously known. Of the resulting 45 localities, 22 correspond to Asturias, 11 to Cantabria and 12 to the Basque Country. Data on the habitat requirements of this plant have been annotated...
An intense exploration of the Spanish Cantabrian coast for the presence or absence of wild Brassica oleracea L., yielded 24 new localities to be added to the 21 previously known. Of the resulting 45 localities, 22 correspond to Asturias, 11 to Cantabria and 12 to the Basque Country. Data on the habitat requirements of this plant have been annotated...
Se citan por primera vez para Argentina Viola micranthella y V. pygmaea, ambas pertenecientes a la
Sección Andinium. La primera se halla en las provincias de Salta y Jujuy mientras que V. pygmaea crece
en Salta. Los taxones se describen e ilustran y se proveen los mapas de distribución geográfica en
Sudamérica y, con mayor detalle, en Argentina
Viola micranthella and V. pygmaea, both entities belonging to section Andinium, are reported for the first time for Argentina. The first one occurs in Salta and Jujuy provinces and the second one in Salta. Both species are described and illustrated and distribution maps are provided.
The experimental material included thirteen bread wheat-breeding lines that carry genes for resistance to M. destruc¬tor and/or H. avenae. The sources of these resistances are the wild species Ae. triuncialis and Ae. ventricosa (lines TR and H-93, respectively) (Delibes et al. 1993, 1997; Romero et al. 1998). We have determined the composition in H...
