Eric M Ziemons

Eric M Ziemons
University of Liège | ulg · Center for Interdisciplinary Research on Medicines - Vibra-Santé Hub

Associate Professor and Vice-director of the CIRM

About

141
Publications
27,936
Reads
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2,876
Citations
Citations since 2017
64 Research Items
1640 Citations
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Introduction
I am currently Associate Professor in Pharmaceutical Analytical Chemistry at the University of Liège and Director of the Vibra-Santé Hub and Vice-director of the Center for Interdisciplinary Research on Medicines (CIRM). My research interests span both vibrational spectroscopy and vibrational imaging applied to the pharmaceutical and biomedical fields with a particular focus on data treatment and surface-enhanced Raman scattering for trace detection in complex media.
Additional affiliations
January 2016 - present
University of Liège
Position
  • Managing Director
January 2013 - present
University of Liège
Position
  • Professor (Associate)
November 2006 - December 2012
University of Liège
Position
  • Senior Researcher
Education
September 2000 - September 2006
University of Liège
Field of study
  • Pharmaceutical Sciences

Publications

Publications (141)
Article
Hyperspectral imaging technology is developing in a very fast way. We find it today in many analytical developments using different spectroscopies for sample classification purposes. Instrumental developments allow us to acquire more and more data in shorter and shorter periods of time while improving their quality. Therefore, we are going in the r...
Article
Full-text available
Quality is one of the essential components of medicines and needs to be ensured to preserve the population’s health. This can be achieved through post-marketing quality control of medicines and is one of the most important duties of national regulatory authorities. In collaboration with the Cameroonian National Drug Quality Control and Valuation La...
Article
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The study of human skin represents an important area of research and development in dermatology, toxicology, pharmacology and cosmetology, in order to assess the effects of exogenous agents, their interaction, their absorption mechanism, and/or their toxicity towards the different cutaneous structures. The processes can be parameterised by mathemat...
Article
The ultimate goal of a one-class classifier like the “rigorous” soft independent modeling of class analogy (SIMCA) is to predict with a certain confidence probability, the conformity of future objects with a given reference class. However, the SIMCA model, as currently implemented often suffers from an undercoverage problem, meaning that its observ...
Article
Full-text available
Quality control is a fundamental and critical activity in the pharmaceutical industry that guarantees the quality of medicines. QC analyses are currently performed using several well-known techniques, mainly liquid and gas chromatography. However, current trends are focused on the development of new techniques to reduce analysis time and cost, to i...
Article
Many active principles belong to the second class of the Biopharmaceutics Classification System due to their low aqueous solubility. Elaboration of new solid oral forms by hot-melt extrusion and fused deposition modeling appears as a promising tool to increase the dissolution rate of these drugs. Indeed, hot-melt extrusion allows the amorphisation...
Article
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Vibrational spectroscopic techniques, i.e., attenuated total reflectance infrared (ATR-IR), near infrared spectroscopy (NIRS) and Raman spectroscopy (RS), coupled with Partial Least Squares Regression (PLSR), were evaluated as cost-effective label-free and reagent-free tools to monitor water content in Levulinic Acid/L-Proline (LALP) (2:1, mol/mol)...
Article
Full-text available
Glycosylation is considered a critical quality attribute of therapeutic proteins as it affects their stability, bioactivity, and safety. Hence, the development of analytical methods able to characterize the composition and structure of glycoproteins is crucial. Existing methods are time consuming, expensive, and require significant sample preparati...
Article
Glyphosate, also known as N-(phosphonomethyl)glycine, is one of the most widely used herbicides in the world. However, the controversy surrounding the toxicity of glyphosate and its main breakdown product, aminomethylphosphonic acid (AMPA), remains a serious public concern. Therefore, there is a clear need to develop a rapid, sensitive and automate...
Article
Full-text available
The study of human skin represents an important area of research and development in dermatology, toxicology, pharmacology and cosmetology, in order to assess the effects of exogenous agents, their interaction, their absorption mechanism, and/or their toxicity towards the different cutaneous structures. The processes can be parameterised by mathemat...
Article
Full-text available
Chemometrics applied to spectroscopic measurements such as near-infrared are gaining more and more importance for quality control of pharmaceutical products. Handheld near-infrared devices show great promise as a medicines quality screening technique for post-marketing surveillance. These devices are able to detect substandard and falsified medicin...
Article
Full-text available
Almost 60% of commercialized pharmaceutical proteins are glycosylated. Glycosylation is considered a critical quality attribute, as it affects the stability, bioactivity and safety of proteins. Hence, the development of analytical methods to characterise the composition and structure of glycoproteins is crucial. Currently, existing methods are time...
Article
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Multivariate curve resolution unmixing of hyperspectral imaging data can be challenging when low sources of variance are present in complex samples, as for minor (low-concentrated) chemical compounds in pharmaceutical formulations. In this work, it was shown how the reduction of hyperspectral imaging data matrices through the selection of essential...
Article
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The objective of this work was to evaluate the impact of physico-chemical properties of pharmaceutical drugs on the optimal mesoporous silica loading methods. Indeed, a good combination between drug and loading process has to be studied to promote the deepest penetration of the drug inside the mesopores, allowing high drug amorphization. Six molecu...
Article
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The distributional homogeneity of chemicals is a key parameter of solid pharmaceutical formulations. Indeed, it may affect the efficacy of the drug and consequently its safety. Chemical imaging offers a unique insight enabling the visualisation of the different constituents of a pharmaceutical tablet. It allows identifying ingredients poorly distri...
Article
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Vitamin D3 is a key micronutrient whose intakes are inadequate for most populations worldwide. Supplementation with medicines or food supplements is commonly prescribed to correct this imbalance and the quality of these products must be ensured. In this context, a generic methodology for the assay of vitamin D3 in oily formulations is proposed usin...
Article
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Cannabis has been at the center of scientific attention for some years now. Since its pharmacological potential has been highlighted, cannabis has become a hot topic in research laboratories, leading to the publication of many scientific studies. Focusing on analytical chemistry, an enormous number of analytical methods for cannabinoid (CNB) determ...
Article
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Analytical quality by design (AQbD) is a systematic approach that allows developing analytical methods in a more science‐based way, due to the higher quality of information collected during the development process. However, its application remains limited to the development of separation methods. (i.e., liquid chromatography and capillary electroph...
Article
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Fluorescence, especially laser induced fluorescence (LIF), is a powerful detection technique thanks to its specificity and high sensitivity. The use of fluorescence detection hyphenated to separation technique often requires the labeling of analytes with suitable fluorescent dye, such as FITC for the labeling of molecules presenting amino groups. N...
Preprint
Full-text available
The optimization of pharmaceutical bioprocesses suffers from several challenges like complexity, upscaling costs, regulatory approval, leading to the risk of delivering substandard drugs to patients. Bioprocess is very complex and requires the evaluation of multiple components that need to be monitored and controlled in order to attain the desired...
Chapter
Full-text available
This chapter covers applications of small, miniature, and portable near‐infrared (NIR) spectrometers in process analytical technology (PAT) in pharmaceutical manufacturing. Continuous manufacturing needs the process to be designed and conceived as a single system. Real‐time monitoring of the process, and the insurance of the quality of the final pr...
Article
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The aim of the present study was to explore the feasibility of applying near-infrared (NIR) spectroscopy for the quantitative analysis of Δ⁹-tetrahydrocannabinol (THC) in cannabis products using handheld devices. A preliminary study was conducted on different physical forms (entire, ground and sieved) of cannabis inflorescences in order to evaluate...
Article
Full-text available
Hyperspectral imaging has been widely used for different kinds of applications and many chemometric tools have been developed to help identifying chemical compounds. However, most of those tools rely on factorial decomposition techniques that can be challenging for large data sets and/or in the presence of minor compounds. The present study propose...
Article
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The main goal of this work was to test the ability of vibrational spectroscopy techniques to differentiate between different polymorphic forms of fluconazole in pharmaceutical products. These are mostly manufactured with fluconazole as polymorphic form II and form III. These crystalline forms may undergo polymorphic transition during the manufactur...
Article
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Synthetic cathinones (SC) are phenylalkylamine compounds related to natural cathinone from Catha Edulis leaves. Due to their sympathomimetic effects comparable to common illicit drugs, these substances are mainly drugs of abuse and constitute the second most frequently seized group of new psychoactive substances (NPS). In order to ensure their regu...
Article
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Essential oils have known a renewed interest, particularly for their antimicrobial properties. In the field of skin delivery of essential oils, fluid oil-in-water (O/W) emulsions have been studied for several years in order to improve their stability. When dealing with infections of the upper skin layers, these vehicles, in spite of their low visco...
Article
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In recent decades, more than 15% of the antimalarials marketed in low- and middle-income countries have been of poor quality, in which quinoline derivatives and quinine-based formulations account for 21%. Near infrared spectroscopy (NIR) was chosen for its fast and inexpensive test properties as well as the ability of using handheld devices to moni...
Article
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The analysis of illicit drugs faces many challenges, mainly regarding the production of timely and reliable results and the production of added value from the generated data. It is essential to rethink the way this analysis is operationalised, in order to cope with the trend toward the decentralization of forensic applications. This paper describes...
Article
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Class-modelling methods aim to predict the conformity of new unknown samples with a single target class, using statistical decision rules built exclusively with objects of that class. This article introduces a novel class-modelling method for spectral data. The method uses the concept of β%-prediction band for functional data to classify spectra. T...
Article
Full-text available
The full text of the paper entitled: The analysis of cannabinoids in cannabis samples by supercritical fluid chromatography and ultra-high-performance liquid chromatography: A comparison study is available at the following website permalink: http://hdl.handle.net/2268/253874 After accepting the ORBI license and if your request is accepted you will...
Article
Full-text available
With the fast growth of bioanalytical surface-enhanced Raman scattering (SERS), analytical methods have had to adapt to the complex nature of biological samples. In particular, interfering species and protein adsorption onto the SERS substrates have been addressed by sample preparation steps, such as precipitation or extraction, and by smart SERS s...
Article
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The proliferation of falsified medicines can cause serious public health issues, particularly in the context of a global pandemic such as the actual COVID-19 pandemic. Our study involved eight chloroquine phosphate medicines seized in Cameroon, Democratic Republic of Congo and Niger during March and May 2020. These suspect samples were first analyz...
Article
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In this study, adipose-derived stem cells (ASCs) are used to produce 3D bone grafts. The safety and the feasibility of using these bone grafts have been already showed and quality controls are already implemented. However, a cheaper, fast and non-destructive technique is required to monitor the osteogenic differentiation process. Here, the use of R...
Article
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Nowadays, the use of functionalised surface-enhanced Raman scattering (SERS) substrates has become common. These surface modifying agents notably act as Raman reporters, as sensors of biological processes (pH, redox probes) or to increase the sensitivity and/or the specificity of SERS detections. However, the effects of the functionalisation agents...
Article
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This paper addresses the issue of pharmaceutical solid dosage form quantitation using handheld Raman spectrophotometers. The two spectrophotometers used are designed with different technologies: one allows getting a more representative sampling with the Orbital Raster Scanning technology and the other one allows setting acquisition parameters. The...
Article
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This study demonstrates, for the first time, the ability of surface-enhanced Raman chemical imaging (SER-CI) combined with multivariate analysis to detect low levels (0.1% (w/w)) of a polymorphic form in a pharmaceutical mixture. In the studied formulation, piroxicam was used as a model molecule to develop this approach. Piroxicam is a widely avail...
Article
Full-text available
Moisture content (MC) is a critical quality attribute of lyophilized biopharmaceuticals and can be determined by near-infrared (NIR) spectroscopy as nondestructive alternative to Karl-Fischer titration. In this study, we create NIR models to determine MC in mAb lyophilisates by use of statistical design of experiments (DoE) and multivariate data an...
Article
Full-text available
Handheld Raman spectroscopy is actually booming. Recent devices improvements aim at addressing the usual Raman spectroscopy issues: fluorescence with shifted‐excitation Raman difference spectroscopy (SERDS), poor sensitivity with surface enhanced Raman scattering (SERS) and information only about the sample surface with spatially offset Raman spect...
Article
Full-text available
Over the last decade, the growth of the global pharmaceutical market has led to an overall increase of substandard and falsified drugs especially on the African market (or emerging countries). Recently, several methods using handheld/portable vibrational spectroscopy have been developed for rapid and on-field drug analysis. The objective of this wo...
Article
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The fast pace of changes occurring in the pharmaceutical world emphasizes the need for powerful technologies that allow checking the quality of pharmaceutical products. Infrared and Raman spectroscopies have shown great potentialities for drug analysis in the last decades and consequently caught the attention of the scientific world as well as of i...
Article
Full-text available
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Article
Full-text available
Hyperspectral imaging has shown a high potential to analyze falsifications of solid pharmaceutical products since the last decade. Thanks to the non-destructive, ecological and non-invasive properties, it is a preferred technique for these kinds of applications. Moreover, thanks to the spectroscopic properties, it is possible to detect as well orga...
Article
Full-text available
This paper addresses the issue of pharmaceutical solid dosage form quantitation using handheld Raman spectrophotometers. The two spectrophotometers used are designed with different technologies: one allows getting a more representative sampling with the Orbital Raster Scanning technology and the other one allows setting acquisition parameters. The...
Article
Full-text available
Background and objectives The kidney stone’s structure might provide clinical information in addition to the stone composition. The Raman chemical imaging is a technology used for the production of two-dimension maps of the constituents' distribution in samples. We aimed at determining the use of Raman chemical imaging in urinary stone analysis. M...
Data
Topological analysis of sample 12. Gaps provide grey to black spots according to their depth, while the whiter zones are associated with more intense Raman signal. (TIF)
Data
Topological analysis of sample 3. Gaps provide grey to black spots according to their depth, while the whiter zones are associated with more intense Raman signal. (TIF)
Data
Topological analysis of sample 9. Gaps provide grey to black spots according to their depth, while the whiter zones are associated with more intense Raman signal. (TIF)
Article
Full-text available
In this study, we describe the development of a SFC-MS method for the quality control of cannabis plants that could be potentially adulterated with synthetic cannabinoids. Considering the high number of already available synthetic cannabinoids and the high rate of development of novel structures, we aimed to develop a generic method suitable for th...
Article
Full-text available
Surface-enhanced Raman chemical imaging (SER-CI) is a highly sensitive analytical tool recently used in the pharmaceutical field owing to the possibility to obtain high sensitivity along with spatial information. However, the covering method of the pharmaceutical samples such as tablets with metallic nanoparticles is a major issue for SER-CI analys...
Article
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The analysis of serum samples by surface-enhanced Raman spectroscopy (SERS) has gained ground over the last few years. However, the stabilisation of colloids by the proteins contained in these samples has restricted their use in common practice, unless antibodies or aptamers are used. Therefore, this work was dedicated to the development of a SERS...
Article
A calibration model for in-line API quantification based on near infrared (NIR) spectra collection during tableting in the tablet press feed frame was developed and validated. First, the measurement set-up was optimised and the effect of filling degree of the feed frame on the NIR spectra was investigated. Secondly, a predictive API quantification...
Article
Full-text available
Although twin screw granulation has already been widely studied in recent years, only few studies addressed the subsequent continuous drying which is required after wet granulation and still suffers from a lack of detailed understanding. The latter is important for optimisation and control and, hence, a cost-effective practical implementation. Ther...
Article
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Near-infrared (NIR) global quantitative models were evaluated for the moisture content (MC) determination of three different freeze-dried drug products. The quantitative models were based on 3822 spectra measured on two identical spectrometers to include variability. The MC, measured with the reference Karl Fischer (KF) method, were ranged from 0.0...
Article
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The development of a quantitative method determining the crystalline percentage in an amorphous solid dispersion is of great interest in the pharmaceutical field. Indeed, the crystalline Active Pharmaceutical Ingredient transformation into its amorphous state is increasingly used as it enhances the solubility and bioavailability of Biopharmaceutica...
Article
Nowadays, many efforts are devoted to improve analytical methods regarding efficiency, analysis time and greenness. In this context, Supercritical Fluid Chromatography (SFC) is often regarded as a good alternative over Normal Phase Liquid Chromatography (NPLC). Indeed, modern SFC separations are fast, efficient with suitable quantitative performanc...
Article
Full-text available
Surface-enhanced Raman spectroscopy (SERS) is a sensitive analytical tool used in the pharmaceutical field in recent years. SERS keeps all the advantages of classical Raman spectroscopy while being is more sensitive allowing its use for the detection and the quantification of low-dose substances contained in pharmaceutical samples. However, the ana...
Article
Full-text available
Since the Food and Drug Administration (FDA) published a guidance based on the Process Analytical Technology (PAT) approach, real-time analyses during manufacturing processes are in real expansion. In this study, in-line Raman spectroscopic analyses were performed during a Hot-Melt Extrusion (HME) process to determine the Active Pharmaceutical Ingr...
Article
Full-text available
In the uprising context of green analytical chemistry, Supercritical Fluid Chromatography is often suggested as an alternative to Normal Phase Liquid Chromatography. Indeed, SFC provides fast, efficient and green separations. In this report, the quantitative performances of SFC were challenged on a real-life case study: the Quality Control (QC) of...
Article
The purpose of this work was to increase the solubility and the dissolution rate of itraconazole, which was chosen as the model drug, by obtaining an amorphous solid dispersion by hot melt extrusion. Therefore, an initial preformulation study was conducted using differential scanning calorimetry, thermogravimetric analysis and Hansen’s solubility p...
Article
Full-text available
The use of Near-infrared spectroscopy (NIRS) as a fast and non-destructive technique was employed for moisture content (MC) determination in Antibody-drug conjugates (ADCs) in replacement to Karl Fischer (KF) method. The lab analysis of ADCs, high potent medicines, should be performed in conditions ensuring the operator’s safety and using secured a...
Article
A new application of surface-enhanced Raman scattering (SERS) in the field of plant material analysis is proposed in this study. The aim was to monitor the release of anatabine by methyl jasmonate (MeJa) elicited Bright Yellow-2 (BY-2) cells. Gold nanoparticles (AuNps) were used as SERS substrate. The first step was to study the SERS activity of an...
Article
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Plant root-knot nematode interaction studies are performed on several host plant models. Though root-knot nematodes interact with trees, no perennial woody model has been explored so far. Here we show that poplar (Populus tremula x P. alba) grown in vitro is susceptible to Meloidogyne incognita allowing this nematode to penetrate, to induce feeding...
Article
Over recent decades, spreading environmental concern entailed the expansion of green chemistry analytical tools. Vibrational spectroscopy, belonging to this class of analytical tool, is particularly interesting taking into account its numerous advantages such as fast data acquisition and no sample preparation. In this context, near-infrared, Raman...
Article
When developing a new formulation, the development, calibration and validation steps of analytical methods based on vibrational spectroscopy are time-consuming. For each new formulation, real samples must be produced and a “reference method” must be used in order to determine the Active Pharmaceutical Ingredient (API) content of each sample. To cir...
Article
The aim of this study was to develop Near Infrared (NIR) methods to determine the active content of non-coated pharmaceutical tablets manufactured from a proportional tablet formulation. These NIR methods intend to be used for the monitoring of the active content of tablets during the tableting process. Firstly, methods were developed in transmissi...
Article
Full-text available
Bisphenol A (BPA) is well known for its use in plastic manufacture and thermal paper production despite its risk of health toxicity as an endocrine disruptor in humans. Since the publication of new legislation regarding the use of BPA, manufacturers have begun to replace BPA with other phenolic molecules such as bisphenol F (BPF) and bisphenol B (B...
Article
Full-text available
The aim of the present study was to optimize a tablet formulation using a quality by design approach. The selected methodology was based on the variation of the filler grade, taking into account the Particle Size Distribution (PSD) of Active Pharmaceutical Ingredient (API) in order to improve five Critical Quality Attributes (CQAs). Thus, a mixture...
Article
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Poor quality antimalarial drugs are one of the public’s major health problems in Africa. The depth of this problem may be explained in part by the lack of effective enforcement and the lack of efficient local drug analysis laboratories. To tackle part of this issue, two spectroscopic methods with the ability to detect and to quantify quinine dihydr...
Article
Newly developed drugs often have poor bioavailability due to their poor water solubility (BCS class 2 drugs). It is therefore necessary to develop new strategies to enhance their solubility and their activity, among which, Self-Emulsifying Drug Delivery System (SEDDS). The efficacy of the drugs contained in these preparations is mainly affected by...
Article
Full-text available
Since the last decade, more and more Active Pharmaceutical Ingredient (API) candidates have poor water solubility inducing low bioavailability. These molecules belong to the Biopharmaceutical Classification System (BCS) class II and IV. Thanks to Hot-Melt Extrusion (HME), it is possible to incorporate these candidates in pharmaceutical solid forms....
Article
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Nowadays, the circulation of poor quality medicines is becoming an alarming worldwide phenomenon with serious public health and socio-economic concerns. The situation is particularly critical in developing countries where drug quality assurance and regulatory systems for drug manufacturing, importation, distribution and sales are weak. A sustained...
Article
A selective and sensitive electroanalytical method was developed for arsenic determination based on a nanogold (AuNP) modified solid carbon paste working electrode (SCPE) modified in two steps (i) physisorption and (ii) additional electrodeposition of nanogold particles in the presence of iodide. Copper(II) interference was solved by covering the g...
Article
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Raman hyperspectral imaging is an increasingly used tool in the pharmaceutical field because it allows for the investigation of many characteristics on a solid sample. This paper delves into Raman spectroscopy and imaging, including spectral and spatial information, and presents some applications of Raman hyperspectral imaging in the pharmaceutical...
Article
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La spectroscopie proche infrarouge est une méthode d’analyse en plein développement. Force est de constater qu’elle est de plus en plus utilisée dans le secteur pharmaceutique pour le contrôle de qualité des produits. En effet, la spectroscopie proche infrarouge permet l’analyse rapide, non destructive, polyvalente des échantillons et ne nécessite...
Article
Full-text available
Les médicaments de la dysfonction érectile constituent une des classes de médicaments les plus contrefaites dans les pays industrialisés. Pour lutter contre ce fléau, différentes approches analytiques sont à la disposition des laboratoires de contrôle. Cet article passe en revue les principales approches utilisées et conclut par la présentation d'u...