About
20
Publications
3,710
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
452
Citations
Introduction
Current institution
Additional affiliations
Zurich University of Applied Sciences, Waedenswil, Switzerland
Position
- Research Associate
Publications
Publications (20)
Microtissues (such as organoids or spheroids) are useful to model healthy or diseased human tissues and organs, with application in drug testing, tissue engineering, and personalized medicine. To characterize their morphology and phenotype, microtissues are often monitored using histology and high-resolution imaging. While this technique is well-es...
Additive manufacturing has been well established in many sectors, including the medical industry. For load-bearing bone implants, titanium and its alloys, such as Ti6Al4V, are widely used due to their high strength to weight ratio and osseointegrative properties. However, bone resorption and loosening of implants is related to the significantly hig...
A key to enhance the low translatability of preclinical drug discovery are in vitro human three-dimensional (3D) microphysiological systems (MPS). Here, we show a new method for automated engineering of 3D human skeletal muscle models in microplates and functional compound screening to address the lack of muscle wasting disease medication. To this...
Orthotopic liver transplantation is presently the most effectual method for the treatment of end‐stage liver diseases. Though, one major issue is the restricted number of donor organs that are accessible. Hence, liver tissue engineering is under investigation with the goal of restoring liver functions. In this study, we investigated 3D porous scaff...
Halogen-free organophosphorus flame retardants are considered as replacements for the phased-out class of polybrominated diphenyl ethers (PBDEs). However, toxicological information on new flame retardants is still limited. Based on their excellent flame retardation potential, we have selected three novel 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10...
Cells grown in 3D are more physiologically relevant than cells cultured in 2D. To use 3D models in substance testing and regenerative medicine, reproducibility and standardization are important. Bioprinting offers not only automated standardizable processes but also the production of complex tissue-like structures in an additive manner. We develope...
Introduction
Thermoreversible hyaluronan (HA) based hydrogels represent a promising carrier for IVD repair because of the mild gel formation allowing for encapsulation of viable cells and biological stimulation of cells by HA. 1,2 Additionally, polyurethane (PU) foams can provide elasticity, which is of key importance for the restoration of IVD mec...
Tissue Engineering as an emerging biotechnology sector aims at the in vitro regeneration of diseased tissues and promises to profoundly change medical practice, offering the possibility of regenerating tissues and organs instead of just repairing them (regenerative medicine).
Improved healing processes and a higher quality of life are the expected...
Nowadays, intervertebral disc (IVD) degeneration is one of the principal causes of low back pain involving high expense within the health care system. The long-term goal is the development of a medical treatment modality focused on a more biological regeneration of the inner nucleus pulposus (NP). Hence, interest in the endoscopic implantation of a...
Nowadays, intervertebral disc (IVD) degeneration is one of the principal causes of low back pain involving high expense within the health care system. The long-term goal is the development of a medical treatment modality focused on a more biological regeneration of the inner nucleus pulposus (NP). Hence, interest in the endoscopic implantation of a...
We previously identified a cold shock domain (CSD)-containing protein (PIPPin), expressed at high level in brain cells. PIPPin has the potential to undergo different posttranslational modifications and might be a good candidate to regulate the synthesis of specific proteins in response to extracellular stimuli. Here we report the effects of T(3) on...
Brain capillary endothelial cells form a functional barrier between blood and brain, based on the existence of tight junctions that limit paracellular permeability. Occludin is one of the major transmembrane proteins of tight junctions and its peripheral localization gives indication of tight junction formation. We previously reported that RBE4.B c...
Questions
Questions (2)
Hello everyone, I’m currently working with human osteosarcoma spheroids for histological analyses. After embedding the spheroids in agarose and infiltrating them, as well as embedding them in paraffin, I’ve noticed that the spheroids sections have a strange structure. They are almost destroyed and present no extracellular matrix (ECM). I’m using a protocol that was established many years ago in our lab, but something seems to be going wrong. I’ve tried to fix my spheroids with formaldehyde, both with and without methanol. I’ve also avoided centrifuging the spheroids in the embedding medium. I’ve tested two different embedding media, 2% agarose in PBS and 2% agarose dissolved in Histogel. However, I keep getting the same strange images. The H&E staining works properly with a control sample (mouse kidney section), so the issue is not due to the staining solutions or protocol. Although I’m not an expert, I’m wondering if the dehydration and infiltration steps could be the problem? Thanks a lot in advance for your support!
Hello everyone, I’m currently working with human osteosarcoma spheroids for histological analyses. After embedding the spheroids in agarose and infiltrating them, as well as embedding them in paraffin, I’ve noticed that the spheroids sections have a strange structure. They are almost destroyed and present no extracellular matrix (ECM). I’m using a protocol that was established many years ago in our lab, but something seems to be going wrong. I’ve tried to fix my spheroids with formaldehyde, both with and without methanol. I’ve also avoided centrifuging the spheroids in the embedding medium. I’ve tested two different embedding media, 2% agarose in PBS and 2% agarose dissolved in Histogel. However, I keep getting the same strange images. The H&E staining works properly with a control sample (mouse kidney section), so the issue is not due to the staining solutions or protocol. Although I’m not an expert, I’m wondering if the dehydration and infiltration steps could be the problem? Thanks a lot in advance for your support!
