Enrico M Bucci

Enrico M Bucci
Temple University | TU · Department of Biology

PhD

About

168
Publications
23,412
Reads
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3,139
Citations
Introduction
Enrico Bucci graduated summa cum laude in Biology in 1997. After 2 years in Germany (IMB, Jena). He earned a Ph.D. in Biochemistry and Molecular Biology in 2001. That year, he was appointed full researcher at the IBB (CNR). Form 2006 to 2008, he was head of R&D at the Bioindustry Park del Canavese. In 2014, he left the CNR. 2 years later, he started Resis Srl, a business devoted to the promotion of research integrity in academic and private environments. Since 2016, he is an adjunct professor in Systems Biology at Temple University in Philadelphia. As a scientist, Enrico Bucci authored about 80 papers in peer-reviewed journals and 2 chapters in 2 different books. His work in the scientific integrity field has been covered by several national and international magazines, including Nature.
Additional affiliations
July 2016 - present
Resis Srl
Position
  • Owner
June 2016 - present
Temple University
Position
  • Professor
January 2010 - June 2016
BioDigitalValley
BioDigitalValley
Position
  • CEO
Education
September 1997 - January 2001
University of Naples Federico II
Field of study
  • Biochemistry, Cellular and Molecular Biology
September 1991 - June 1997
September 1986 - June 1991
Liceo "Antonio Genovesi"
Field of study
  • Classical studies

Publications

Publications (168)
Article
Analysis of images obtained from two-dimensional gel electrophoresis (2-D GE) is a topic of utmost importance in bioinformatics research, since commercial and academic software currently available have proven to be neither completely effective nor fully automatic, often requiring manual revision and refinement of computer generated matches. In this...
Article
Full-text available
A number of commercial software packages are currently available to perform digital two-dimensional electrophoresis (2D-GE) gel analysis. However, both the high cost of the commercial packages and the unavailability of a standard data analysis workflow, have prompted several groups to develop freeware systems to perform certain steps of gel analysi...
Article
Full-text available
Introduction: Acute lung injury and acute respiratory distress syndrome are common complications in patients with coronavirus disease 2019 (COVID-19). Poor outcomes in patients with COVID-19 are associated with cytokine release syndrome. Binding of interleukin-8 (CXCL8/IL-8) to its chemokine receptors, CXCR1/2, may mediate this inflammatory proces...
Article
Full-text available
The new coronavirus SARS-CoV-2 is the causative agent of the COVID-19 pandemic, which so far has caused over 6 million deaths in 2 years, despite new vaccines and antiviral medications. Drug repurposing, an approach for the potential application of existing pharmaceutical products to new therapeutic indications, could be an effective strategy to ob...
Article
Scientific fraud still lacks a precise, universally accepted definition: the borders between unambiguously established fraud, errors, misconduct are uncertain: this frequently complicates decisions on whether or not cases of questionable behaviour can be classified as true fraud. In this article we have listed the behaviours which establish that tr...
Preprint
Full-text available
The new coronavirus that emerged, called SARS-CoV-2, is the causative agent of the COVID-19 pandemic. The identification of potential drug candidates that can rapidly enter clinical trials for the prevention and treatment of COVID-19 is an urgent need, despite the recent introduction of several new vaccines for the prevention and protection of this...
Article
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The ongoing coronavirus disease 2019 (COVID-19) pandemic caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) necessitates strategies to identify prophylactic and therapeutic drug candidates to enter rapid clinical development. This is particularly true, given the uncertainty about the endurance of the immune memory indu...
Article
Full-text available
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spreads mainly via aerosol (microdroplets) in enclosed environments where temperature and humidity are regulated via air-conditioning (A/C). About 30% of individuals infected with SARS-CoV-2 develop COVID-19 disease. Among them, ∼25% require hospitalization. In medicine “cases” identify t...
Article
Full-text available
Author Francesca Pentimalli was incorrectly associated with Histopathological Unit, IRCCS-Istituto Tumori “Giovanni Paolo II”, Viale Orazio Flacco 65, 70124 Bari, Italy. The author’s actual affiliation is Cell Biology and Biotherapy Unit, Istituto Nazionale Tumori-IRCCS-Fondazione G. Pascale, I-80131 Napoli, Italy.
Article
Full-text available
Malignant pleural mesothelioma (MPM) is an aggressive cancer, related to asbestos exposure, which has a dismal prognosis. MPM diagnosis is late and often challenging, suggesting the need to identify more reliable molecular biomarkers. Here, we set out to identify differentially expressed miRNAs in epithelioid, biphasic, and sarcomatoid MPMs versus...
Article
Full-text available
Coronavirus disease 2019 (COVID-19) is a severe acute respiratory syndrome caused by Coronavirus 2 (SARS-CoV-2). In the light of its rapid global spreading, on 11 March 2020, the World Health Organization has declared it a pandemic. Interestingly, the global spreading of the disease is not uniform, but has so far left some countries relatively less...
Article
The agent of Covid19, Sars-CoV-2 has caused thousands of fatalities worldwide and overshadowed the number of deaths of other previous coronavirus outbreaks (Sars-CoV1 2002 and MERS-CoV 2012). Although the new coronavirus pathogenicity is actively under investigation, part of its infectious behavior can be linked to its higher binding affinity to th...
Article
Honest errors, sloppy practices or true frauds: there can be several causes of inconsistences and problematic data in a scientific paper. However, whatever the actual outcome of an investigation into a case of alleged data manipulation, there are certain firm principles that always hold true, and certain errors that must be avoided.
Article
In Italy, in the eastern area of the Campania region, the illegal dumping and burning of waste have been documented, which could potentially affect the local population's health. In particular, toxic waste exposure has been suggested to associate with increased cancer development/mortality in these areas, although a causal link has not yet been est...
Article
In 2013, an outbreak of Xylella fastidiosa was identified for the first time in Europe, in the extreme South of Italy (Apulia, Salento territory). The locally identified subspecies turned to be lethal for olive trees, starting an unprecedented phytosanitary emergency for one of the most iconic cultivations of the Mediterranean area. The Apulian-mon...
Article
Full-text available
The development of specific and individualized training programs is a possible way to improve athletic performance and minimize injuries in professional athletes. The information regarding the sport's physical demands and the athletes' physical profile have been, so far, considered as exhaustive for the design of effective training programs. Howeve...
Article
In July 2018, two different requests for an in-depth investigation of the Olive Quick Decline Syndrome epidemic in our country - an act of inspective syndicate by the Parliament and a request for the establishment of a commission of inquiry by the Senate – made very clear that politicians, or at least the authors of such requests, were still sticki...
Article
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Chapter
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420 Per lungo tempo, in Europa, il finanziamento e la pro-mozione della ricerca scientifica e dell'attività cultu-rale in genere sono rimasti appannaggio di singoli me-cenati o gruppi di potere, i quali selezionavano i migliori 'dilettanti' disponibili, ovvero quegli indivi-dui che per proprio diletto coltivavano questo o quel settore del sapere. L...
Presentation
Full-text available
Recently, Scortichini et al. reported the efficacy of Dentamet, a copper-zinc fertilizer, in the treatment of X.fastidiosa infected olive trees in Southern Italy. However, after reading the paper I have several doubts on the real efficacy of the compound and on the study setting, as explained in the presentation.
Presentation
Full-text available
La medicina basata sulle evidenze (EBM) è certamente stata una dei maggiori progressi nel modo in cui la malattia e la terapia sono affrontate. La raccolta di dati e la loro analisi è al cuore di tale approccio, che informa tutti i processi di preparazione delle informazioni poi utilizzate dai medici sia nel confronto diretto con i pazienti che nel...
Article
Full-text available
Images in scientific papers are used to support the experimental description and the discussion of the findings since several centuries. In the field of biomedical sciences, in particular, the use of images to depict laboratory results is widely diffused, at such a level that one would not err in saying that there is barely any experimental paper d...
Presentation
Full-text available
Article
Nowadays, since scientific fraud is perceived as a serious societal threat, it is quite easy to find articles which pose the question of whether research misconduct should be considered a criminal offence and how would it be possible to proceed in a criminal trial against fraudsters. When discussing these topics, however, it happens to forget that...
Article
Several tools and methods are currently explored to face the explosion of scientifc misconduct. While most deal with the discovery of misbehavior and punishment of those researchers recognized as guilty, there were few attempts to work on the side of prevention, mostly focused on education. In the feld of medicine, the moral binding strength of a d...
Presentation
Full-text available
Presentation given at XV CONGRESS OF SIF & ICEMAP 2017
Article
All'inizio di quest'anno la Commissione ricerca dell'Accademia nazionale dei Lincei ha chiesto all'autore di valutare se vi fossero dati sufficienti per provare in maniera solida la correlazione tra infezione del batterio Xylella fastidiosa osservata in olivi pugliesi e presenza di sintomi di della sindrome da disseccamento rapido. Lo studio ha rig...
Article
Recent aggressive chemotherapeutic and combined treatments have resulted in increased survivorship for advanced stage breast cancer. In some patients, treatment produces an actual abatement of their cancer, while in others treatment mitigates the progression of cancer bringing those patients into palliative care where their chronic disease requires...
Article
Il grande vantaggio dell'indagine scientifica sopra il mondo fisico rispetto a possibili alternative di natura filosofica, religiosa o di ogni altra specie che sia stata finora immaginata, consiste principalmente nell'utilizzare un metodo che, almeno in potenza, è in grado intrinsecamente di individuar debolezze e inconsistenze nel sapere accumulat...
Presentation
Full-text available
Invited Talk at the Institute of Systems Analysis and Computer Science ”A. Ruberti” (IASI-CNR)
Presentation
Full-text available
This is the analysis of possible manipulations in the papers of the group led by prof. Infascelli. It was referred to by nature (http://www.nature.com/news/italian-papers-on-genetically-modified-crops-under-investigation-1.19183)
Book
Quando ho iniziato a lavorare nel settore dell’analisi dei dati biomedici ero convinto che la Scienza si autocorreggesse e che i ricercatori fossero motivati nel dire sempre la verità. Nelle discipline scientifiche ogni ricercatore ha fiducia e si appoggia ai risultati ottenuti da altri per le proprie particolari indagini, il che era ciò che intend...
Presentation
Full-text available
When I started my business in biomedical data analysis, I was fully convinced that Science was able to autocorrect itself – and researchers as a community were motivated into telling the truth, notwithstanding some notable exception. In Science, every researchers build upon trusted results obtained by others; which was exactly what I wanted to do,...
Presentation
Full-text available
L’immagine scientifica, e nella fattispecie i diversi tipi di immagine acquisiti attraverso macchinari via via più sofisticati, lungi dall’essere una semplice illustrazione, è stata ormai assunta come una delle possibili forme del dato scientifico, utilizzabile per dimostrare una gran varietà di fenomeni e per misurare una quantità di variabili spe...
Article
Constitutively active receptor tyrosine kinases (RTKs) are known oncogenic drivers and provide valuable therapeutic targets in many cancer types. However, clinical efficacy of RTK inhibitors is limited by intrinsic and acquired resistance. To identify genes conferring resistance to inhibition of the MET RTK, we conducted a forward genetics screen i...
Article
Alice is an engineer who ventures into the research world of life sciences. To her eyes, life sciences researchers work backwards compared to what happens in her world. It appears that their research methodology has a number of issues that may limit its potential. Nevertheless, she also becomes aware that a different set of problems arises if her o...
Article
Discusses the technologies and applications supported by nanotechnology, considered one of the 21st century's most promising technologies. Nanotechnology deals with the design, development, and manipulation of materials and devices with at least one dimension sized on a nanometer scale. It involves fields of science as diverse as surface science, o...
Article
Amyotrophic Lateral Sclerosis (ALS) is a progressive, fatal neurodegenerative disease caused by the degeneration of motor neurons. The transgenic mouse model carrying the human SOD1G93A mutant gene (hSOD1G93A mouse) represents one of the most reliable and widely used model of this pathology. In the present work, the innovative technique of matrix-a...
Conference Paper
Data as a Service (DaaS) is among the latest kind of services being investigated in the Cloud computing community. The main aim of DaaS is to overcome limitations of state-of-the-art approaches in data technologies, according to which data is stored and accessed from repositories whose location is known and is relevant for sharing and processing. B...
Article
iMole is a platform that automatically extracts images and captions from biomedical literature. Images are tagged with terms contained in figure captions by means of a sophisticate text mining tool. Moreover iMole allows the user to upload directly their own images within the database and manually tag images by curated dictionary. Using iMole the r...
Conference Paper
Data as a Service (DaaS) is among the latest kind of services being investigated in the Cloud computing community. The main aim of DaaS is to overcome limitations of state-of-the-art approaches in data technologies, according to which data is stored and accessed from repositories whose location is known and is relevant for sharing and processing. B...
Article
We here report the design, synthesis, and biological activity of a kinked hairpin-loop DNA acting at low nM concentrations as a strong inhibitor of HMGB1 (High-Mobility Group Box-1), a nuclear protein with cytokine activity in a number of inflammatory diseases. Lead compound optimization has been realized by inserting different oligo-ethylene glyco...
Article
Hepatocyte Growth Factor (HGF) controls growth and differentiation in different cell types, including cardiac cells. However, its downstream effectors are poorly understood. To investigate the transcriptional targets of HGF, we analyzed the hearts of neonatal mice with cardiomyocyte-specific HGF overexpression with whole genome DNA microarrays. Whe...
Data
and 3 and nucleopeptide 4, respectively 264 kb)
Article
Full-text available
In this work, we report a synthetic approach to a Fmoc-protected nucleoamino acid, based on L: -diaminopropanoic acid, carrying the DNA nucleobase on the alpha-amino group by means of an amide bond, suitable for the solid-phase synthesis of novel nucleopeptides of potential interest in biomedicine. After ESI-MS and NMR characterization this buildin...
Conference Paper
Full-text available
Analysis of 2D-GE images is a hot topic in bioinformatics research, since currently available commercial and academic software has proven to be not really effective and not completely automatic, often requiring manual revision of spots detection and refinement of computer generated matches. In this work, we present an effective technique for the de...
Article
Full-text available
This work deals with the Dakin-West synthesis, starting from the nucleoamino acid 1-thyminyl acetic acid, as well the NMR, ESI MS, and X-ray characterization of a heteroaromatic compound denominated by us T(2)CO, comprising two thymine moieties anchored to a 2-propanonic unit, the spectroscopic properties of which were studied by UV as a function o...
Article
In the present work, we report the conjugation of superparamagnetic nanoparticles to a fluorescently labeled oligodeoxyribonucleotide (ODN) able to fold into stable unimolecular guanine quadruple helix under proper ion conditions by means of its thrombin-binding aptamer (TBA) sequence. The novel modified ODN, which contained a fluorescent dU(Py) un...
Article
Full-text available
In this work, we describe the synthesis, evaluation of some biological properties, such as DNA- and RNA-binding ability and in sero stability, as well as the supramolecular assembly of a novel nucleoamino acid based on L-spinacine. More particularly, a thymine-containing L-spinacine derivative was synthesized in liquid phase by a simple peptide-cou...
Article
This paper reviews the possibilities offered by the advent of nanotechnology for regulating the interaction between electromagnetic fields and bio-systems with unprecedented flexibility and specificity. In particular, we discuss how the exploitation of magnetic nanoparticles and magnetic fields can lead to significant advances in clinical and diagn...
Article
Among heavy metals, whose toxicity cause a steadily increasing of environmental pollution, cadmium is of special concern due to its relatively high mobility in soils and potential toxicity at low concentrations. Given their ubiquitous role, zinc fingers domains have been proposed as mediators for the toxic and carcinogenic effects exerted by xenobi...
Article
The main aim of this paper is to set the theoretical framework needed for the analysis and design of magnetically driven nanomachines, namely, hybrid nanosystems obtained by assembling macromolecular complexes and magnetic nanoparticles (MNPs), whose functions can be activated and controlled by means of magnetic fields (MFs). Starting from the theo...
Conference Paper
Hepatoma-derived growth factor related protein 3 (HDGFRP3) belongs to the HDGF polypeptide family. Expression of HDGF is a prognostic factor for NSCLC, pancreatic and gastrointestinal tumors, and silencing of HDGF suppressed cell proliferation, migration and invasion of squamous lung cancer cells. Here we investigated the expression of HDGFRP3 in v...
Article
This work concerns a dithymine tetrapeptide, which can be seen as a new analogue of a dinucleoside monophosphate, made of both unfunctionalized and thymine-containing L-serine units alternated in the sequence. The new nucleopeptide was obtained on the solid phase by two different synthetic strategies. The first one is suitable to easily realize nuc...
Article
A novel cationic peptide based on L-lysine and L-diaminobutyric acid was prepared for the first time by solid phase synthesis. After HPLC purification and ESI MS characterization, we studied by CD and IR spectroscopy the structural features of the novel basic peptide, which is able to form a β-turn-like structure. Furthermore, its interaction with...
Article
Full-text available
In this work we report the design and synthesis of kinked oligonucleotide duplexes as potential inhibitors of HMGB1, a cytokine which triggers a broad range of immunological effects. We found that the designed ligands can interact with HMGB1, as evidenced by circular dichroism spectroscopy, and are able to block some extracellular effects induced b...
Article
This work concerns the synthesis, the supramolecular assembly and the evaluation of some biological properties, such as DNA and RNA-binding ability and human serum stability, of novel nucleopeptides. These compounds are of potential interest for the well-known properties that similar compounds, such as natural peptidyl nucleosides, possess in biolo...
Conference Paper
Full-text available
Proteomics has gained a wide interest in the last decade since it involves the comparative study of protein expressions to identify bio-markers for early diagnosis of unpredictable, and serious, pathologies. The most powerful techniques for protein investigation compare 2D gel electrophoresys images that represent the protein composition of healthy...
Article
Full-text available
Haptoglobin is an acute phase glycoprotein, secreted by hepatocytes and other types of cells including keratinocytes. Haptoglobin has been suggested to impair the immune response, inhibit gelatinases in the extracellular matrix and promote angiogenesis, but its role in psoriasis is obscure to date. Changes in haptoglobin glycan structure were obser...