Emmanuelle M F Bayer

Emmanuelle M F Bayer
French National Centre for Scientific Research | CNRS · Institute of Plant Science

About

74
Publications
18,165
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4,156
Citations
Citations since 2016
40 Research Items
2593 Citations
20162017201820192020202120220100200300400
20162017201820192020202120220100200300400
20162017201820192020202120220100200300400
20162017201820192020202120220100200300400

Publications

Publications (74)
Article
Full-text available
Plasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later dynamic regulation. Here we provide a biochemical characterisation of the cell wall co-purified with primary PD of Arabidopsis thali...
Article
Despite recent progress in our understanding of graft union formation, we still know little about the cellular events underlying the grafting process. This is partially due to the difficulty of reliably targeting the graft interface in electron microscopy to study its ultrastructure and three-dimensional architecture. To overcome this technological...
Preprint
Despite recent progress in our understanding of the graft union formation, we still know little about the cellular events underlying the grafting process. This is partially due to the difficulty of reliably targeting the graft interface in electron microscopy to study its ultrastructure and three-dimensional architecture. To overcome this technolog...
Preprint
Full-text available
Iron is critical for host-pathogen interactions. While pathogens seek to scavenge iron to spread, the host aims at decreasing iron availability to reduce pathogen virulence. Thus, iron sensing and homeostasis are of particular importance to prevent host infection and part of nutritional immunity. While the link between iron homeostasis and immunity...
Preprint
Full-text available
Iron is critical for host-pathogen interactions. While pathogens seek to scavenge iron to spread, the host aims at decreasing iron availability to reduce pathogen virulence. Thus, iron sensing and homeostasis are of particular importance to prevent host infection and part of nutritional immunity. While the link between iron homeostasis and immunity...
Article
Full-text available
Plasmodesmata are intercellular pores connecting together most plant cells. These structures consist of a central constricted form of the endoplasmic reticulum, encircled by some cytoplasmic space, in turn delimited by the plasma membrane, itself ultimately surrounded by the cell wall. The presence and structure of plasmodesmata create multiple rou...
Preprint
Full-text available
Plant growth and development are modulated by both biotic and abiotic stress. Increasing evidence suggests that cellular integration of the corresponding signals occurs within pre-formed hubs at the plasma membrane called nanodomains. These membrane sub-compartments are organized by multivalent molecular scaffold proteins, such as remorins. Here, w...
Article
Full-text available
The vast majority of cellular processes take root at the surface of biological membranes. By providing a two-dimensional platform with limited diffusion, membranes are, by nature, perfect devices to concentrate signaling and metabolic components. As such, membranes often act as "key processors" of cellular information. Biological membranes are high...
Article
Full-text available
Intercellular communication plays a crucial role in the establishment of multicellular organisms by organizing and coordinating growth, development and defence responses. In plants, cell- to-cell communication takes place through nanometric membrane channels called plasmodesmata (PD). Understanding how PD dictate cellular connectivity greatly depen...
Preprint
Full-text available
During phloem unloading, multiple cell-to-cell transport events move organic substances to the root meristem. Although the primary unloading event from the sieve elements to the phloem pole pericycle has been characterized to some extent, little is known about post-sieve element unloading. Here, we report a novel gene, PHLOEM UNLOADING MODULATOR (P...
Article
Full-text available
Plasmodesmata pores control the entry and exit of molecules at cell-to-cell boundaries. Hundreds of pores perforate the plant cell wall, connecting cells together and establishing direct cytosolic and membrane continuity. This ability to connect cells in such a way is a hallmark of plant physiology and is thought to have allowed sessile multicellul...
Article
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An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Article
Full-text available
Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses. While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activi...
Article
Full-text available
During phloem unloading, multiple cell-to-cell transport events move organic substances to the root meristem. Although the primary unloading event from the sieve elements to the phloem pole pericycle has been characterized to some extent, little is known about post-sieve element unloading. Here, we report a novel gene, PHLOEM UNLOADING MODULATOR (P...
Preprint
Full-text available
Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development and responses to environmental stresses. While many of the developmental, biotic and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activiti...
Article
Full-text available
Understanding the mode of action of membrane contact sites (MCSs) across eukaryotic organisms at the near-atomic level to infer function at the cellular and tissue levels is a challenge scientists are currently facing. These peculiar systems dedicated to inter-organellar communication are perfect examples of cellular processes where the interplay b...
Data
Analysis of in vitro 6His-REM1.3 phosphorylation and viral proteins expression. (A) Effect of the addition of ATP or AMP in in vitro phosphorylation assays of 6His-REM1.3 by kinase(s) in microsomal (μ) or PM extracts of N. benthamiana leaves developed by autoradiography. (B) 6His-REM1.3N and 6His-REM1.3 phosphorylation by healthy N. benthamiana lea...
Data
In vitro characterization of REM1.3 phosphorylation conditions. Autoradiography reveals in vitro phosphorylated 6His-REM1.3N (A) or 6His-REM1.3 (B) by microsomal extracts of healthy N. benthamiana leaves in the presence of increasing concentrations of staurosporine (A) or Polylysine, β-glycerophosphate (BGP), GTP, AMP and ATP (B). (C) Effect of Ca2...
Data
AtCPK3CAD202A dead mutant does not phosphorylate REM1.3 in vitro. AtCPK3CA-HA and AtCPK3CAD202A-HA were expressed in Arabidopsis thaliana mesophyll protoplasts. Immunoprecipitated proteins were incubated with ATP [γ-33P] and submitted to an in vitro kinase assay using 6His-REM1.3 or histone as substrates. In vitro kinase assays were revealed by aut...
Data
REM1.3 S74 T86 S91 phosphorylation is important to regulate Tobacco mosaic virus movement and REM1.3 phosphorylation mutants maintain PM localization. (A) Representative epifluorescence microscopy images of Tobacco Mosaic Virus (TMV-GFP) infection foci in N. benthamiana leaf epidermal cells at 5 DAI. Graph represents the relative foci area of REM1....
Data
Overexpression of GFP-REM1.3 results in reduced PVX accumulation in N. benthamiana and REM1.3 protein levels are not affected by PVX infection. A. Top, Confocal image showing GFP-REM1.3 localisation in the PM in N. benthamiana lines Bottom, The GFP:REM1.3 expression in three independent transgenic lines #6, 7 and 16 was tested by Western blot again...
Data
Group 1b AtREMs and REM1.3 have similar behavior against PVX cell-to-cell movement in N. benthamiana epidermal cells. (A) Clustal alignments of protein sequences of group 1b REMORINs: AtREM1.2, AtREM1.3, NbREM1.2, NbREM1.3 and REM1.3 (StREM1.3). Blue color-coding shows percentage of identity. The REM1.3 S74, T81 and S91 sites are highlighted. (B) L...
Data
Callose quantification by aniline blue staining and PD index calculation. (A) Original sample image is an 8-bit, single-channel image. (B) Masks of total Region Of Interest (ROI) objects before particle analysis were created using the following filters; background subtraction with a rolling ball radius as in [43]; “smooth” twice and an auto-local t...
Data
Stable transgenic lines N. benthamiana under-expressing group 1 REMORINs. (A) Protein expression levels of endogenous NbREMs in the hpREM lines, determined by Western Blot analysis using anti-REM1.3 antibodies. Protein extracts from three independent plants per line were used, namely lines 1.4, 2.1, 10.2. (B) Expression of endogenous NbREMs in the...
Article
Full-text available
Plants respond to pathogens through dynamic regulation of plasma membrane-bound sig-naling pathways. To date, how the plant plasma membrane is involved in responses to viruses is mostly unknown. Here, we show that plant cells sense the Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins and subsequently trigger the activation of a me...
Preprint
Full-text available
A bstract In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved unique MCS, the plasmodesmata intercellular pores, which combine endoplasmic reticulum (ER) - plasma membrane (PM) contacts with regulation of cell-to-cell signalling. The molecular mechanism and function of membrane tethering wi...
Article
Full-text available
In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved unique MCS, the plasmodesmata intercellular pores, which combine endoplasmic reticulum (ER) - plasma membrane (PM) contacts with regulation of cell-to-cell signalling. The molecular mechanism and function of membrane tethering within plasm...
Article
Full-text available
Plasmodesmata (PD) are nanometric (similar to 20 nm wide) membrane lined pores encased in the cell walls of the adjacent plant cells. They allow the cells to exchange all types of molecules ranging from nutrients like sugar, hormones, to RNAs and various proteins. Unfortunately, they are also hijacked by phyto-viruses, enabling them to spread from...
Preprint
Full-text available
Plants respond to pathogens through dynamic regulation of plasma membrane-bound signaling pathways. To date, how the plant plasma membrane is involved in responses to viruses is mostly unknown. Here, we show that plant cells sense the Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins and subsequently trigger the activation of a mem...
Chapter
Plasmodesmata (PD) are plasma membrane lined pores that cross the plant cell wall and connect adjacent cells. Plasmodesmata are composed of elements of the endoplasmic reticulum, plasma membrane, cytosol, and cell wall and thus, as multicomposite structures that are embedded in the cell wall, they are notoriously difficult to isolate from whole pla...
Article
The plant endoplasmic reticulum (ER) defines the biosynthetic site of lipids and proteins destined for secretion, but also contains important signal transduction and homeostasis components that regulate multiple hormonal and developmental responses. To achieve its various functions, the ER has a unique architecture, both reticulated and highly plas...
Article
Full-text available
Plasma Membrane is the primary structure for adjusting to ever changing conditions. PM sub-compartmentalization in domains is thought to orchestrate signaling. Yet, mechanisms governing membrane organization are mostly uncharacterized. The plant-specific REMORINs are proteins regulating hormonal crosstalk and host invasion. REMs are the best-charac...
Article
Full-text available
Plasmodesmata are remarkable cellular machines responsible for the controlled exchange of proteins, small RNAs and signalling molecules between cells. They are lined by the plasma membrane (PM), contain a strand of tubular endoplasmic reticulum (ER), and the space between these two membranes is thought to control plasmodesmata permeability. Here, w...
Article
Full-text available
Plasmodesmata (PD) are a hallmark of the plant kingdom and a cornerstone of plant biology and physiology, forming the conduits for the cell-to-cell transfer of proteins, RNA and various metabolites, including hormones. They connect the cytosols and endomembranes of cells, which allows enhanced cell-to-cell communication and synchronization. Because...
Article
Full-text available
Plasmodesmata (PD) are a hallmark of the plant kingdom and a cornerstone of plant biology and physiology, forming the conduits for the cell-to-cell transfer of proteins, RNA and various metabolites, including hormones. They connect the cytosols and endomembranes of cells, which allows enhanced cell-to-cell communication and synchronization. Because...
Article
The coordination of multiple metabolic activities in plants relies on an interorganelle communication network established through membrane contact sites (MCS). The MCS are maintained in transient or durable configurations by tethering structures which keep the two membranes in close proximity, and create chemical microdomains that allow localized a...
Article
Full-text available
Multicellularity differs in plants and animals in that the cytoplasm, plasma membrane, and endomembrane of plants are connected between cells through plasmodesmal pores. Plasmodesmata (PDs) are essential for plant life and serve as conduits for the transport of proteins, small RNAs, hormones, and metabolites during developmental and defense signali...
Article
Full-text available
Lipid composition of plasma membrane (PM) and the corresponding Detergent-Insoluble Membrane (DIM) fraction were analyzed with a specific focus on highly polar sphingolipids so-called Glycosyl-Inositol-Phosphoryl-Ceramides (GIPCs). Using tobacco Bright Yellow-2 cell suspension and tobacco leaves (Nicotiana tabacum), evidences were provided that GIP...
Article
Full-text available
Due to their position firmly anchored within the plant cell wall, plasmodesmata (PD) are notoriously difficult to isolate from plant tissue. Yet, getting access to isolated PD represents the most straightforward strategy for the identification of their molecular components. Proteomic and lipidomic analyses of such PD fractions have provided and wil...
Article
Full-text available
Plasmodesmata (PD) are nano-sized membrane-lined channels controlling intercellular communication in plants. Although progress has been made in identifying PD proteins, the role played by major membrane constituents, such as the lipids, in defining specialized membrane domains in PD remains unknown. Through a rigorous isolation of "native" PD membr...
Article
Full-text available
Plasmodesmata (PD) are plant-specific membrane-lined channels that connect neighboring cells across the cell wall and are indispensable for intercellular communication, development and defense against pathogens. They consist of concentric membrane tubules of the plasma membrane (PM) on the outside and endoplasmic reticulum (ER) on the inside. The b...
Article
The Triple Gene Block 1 (TGBp1) protein encoded by the Potato virus X is a multifunctional protein that acts as a suppressor of RNA silencing or facilitates the passage of virus from cell to cell by promoting the plasmodesmata opening. We previously showed that the membrane raft protein StRemorin1.3 is able to impair PVX infection. Here, we show th...
Article
Full-text available
Phyllotaxis, the regular arrangement of leaves and flowers around the stem, is a key feature of plant architecture. Current models propose that the spatiotemporal regulation of organ initiation is controlled by a positive feedback loop between the plant hormone auxin and its efflux carrier PIN-FORMED1 (PIN1). Consequently, pin1 mutants give rise to...
Article
Full-text available
Although genetic control of morphogenesis is well established, elaboration of complex shapes requires changes in the mechanical properties of cells. In plants, the first visible sign of leaf formation is a bulge on the flank of the shoot apical meristem. Bulging results from local relaxation of cell walls, which causes them to yield to internal hyd...
Article
Full-text available
In plants, the intercellular communication through the membranous channels called plasmodesmata (PD; singular plasmodesma) plays pivotal roles in the orchestration of development, defence responses, and viral propagation. PD are dynamic structures embedded in the plant cell wall that are defined by specialized domains of the endoplasmic reticulum (...
Article
The dynamic segregation of membrane components within microdomains, such as the sterol-enriched and sphingolipid-enriched membrane rafts, emerges as a central regulatory mechanism governing physiological responses in various organisms. Over the past five years, plasma membrane located raft-like domains have been described in several plant species....
Data
PD-proteome with Mapman Bin functional categories and predicted information on subcellular localization and description of proteins in the Public Proteome collection (PPDB) (ProteomicsPub. Column). a CHLOR = Chloroplast protein; MIT = Mitochondrial protein; VACUOL = Vacuolar protein; S = Secreted; SM = Secretory membrane; NSnoTM = Nonsecreted no tr...
Data
Gene ontology (GO) terms for the predicted functional categorization of the PD-proteome. The three main subcategories are represented: Cellular components (A), Molecular function (B) and Biological Processes (C). (TIF)
Data
Colocalisation of fluorescent puncta with callose. Leaf tissues stably expressing fluorescent protein fusions (left panel) were stained with aniline blue (centre panel) to identify sites of callose deposition. Colocalisation of the fluorescence (right panel) supports these fluorescent puncta as the location of PD on the wall. Similar patterns of st...
Data
Complete list of PD-proteome sequence identities (1341) with associated the proteomic information. aIt should be noted that when paralogous proteins could not be distinguished, all were included. (XLS)
Data
PD-proteome with Gene Ontology descriptions. a Comp = Cellular component; Proc = Biological processes; Func = Functional categories. RAW Mascot files have been lodged with TRANCHE (https://proteomecommons.org/tranche/), and protein and peptide identifications with associated spectra have been lodged with ‘PRIDE’ (http://www.ebi.ac.uk/pride/easySubm...
Article
The multicellular nature of plants requires that cells should communicate in order to coordinate essential functions. This is achieved in part by molecular flux through pores in the cell wall, called plasmodesmata. We describe the proteomic analysis of plasmodesmata purified from the walls of Arabidopsis suspension cells. Isolated plasmodesmata wer...
Article
Full-text available
The multicellular nature of plants requires that cells should communicate in order to coordinate essential functions. This is achieved in part by molecular flux through pores in the cell wall, called plasmodesmata. We describe the proteomic analysis of plasmodesmata purified from the walls of Arabidopsis suspension cells. Isolated plasmodesmata wer...
Article
Over the past five years, the structure, composition and possible functions of membrane raft-like domains on plant plasma membranes (PM) have been described. Proteomic analyses have indicated that a high proportion of proteins associated with detergent-insoluble membranes (DIMs), supposed to contain raft-like domains isolated from the PM, might be...
Article
Full-text available
Remorins are plant-specific proteins found associated with plasma membrane microdomains, called lipid rafts. Recently, we have shown that this lipid raft marker also accumulates at plasmodesmata, likely within the plasma membrane lining these structures. Here, we have investigated the gene expression and protein accumulation patterns of remorin at...
Article
Full-text available
One of the most striking aspects of plant diversity is variation in leaf shape. Much of this diversity is achieved by the modulation of leaf blade dissection to form lobes or leaflets. Here, we show that the phytohormone auxin is a crucial signal regulating the partitioned outgrowth necessary to develop a dissected leaf. In developing leaves, the a...
Article
Many patterning events in plants are regulated by the phytohormone auxin. In fact, so many things are under the influence of auxin that it seems difficult to understand how a single hormone can do so much. Auxin moves throughout the plant via a network of specialized membrane-bound import and export proteins, which are often differentially expresse...
Article
Full-text available
Remorins (REMs) are proteins of unknown function specific to vascular plants. We have used imaging and biochemical approaches and in situ labeling to demonstrate that REM clusters at plasmodesmata and in approximately 70-nm membrane domains, similar to lipid rafts, in the cytosolic leaflet of the plasma membrane. From a manipulation of REM levels i...
Article
In Arabidopsis thaliana, auxin is a key regulator of tissue patterning in the developing embryo. We have identified a group of proteins that act downstream of auxin accumulation in auxin-mediated root and vascular development in the embryo. Combined mutations in OBERON1 (OBE1) and OBERON2 (OBE2) give rise to obe1 obe2 double mutant seedlings that c...