Emmanuel Martínez-Montaño

• Dr.
• CONAHCYT - UPSIN at Polytechnic University of Sinaloa

Yellowfin tuna (Thunnus albacares) is one of the most captured species for industrial processing and their viscera usually are considered a waste that represents a low-cost source of proteolytic enzymes. Thus, the stability of crude alkaline proteases extracted from yellowfin tuna intestinal tissues, to several denaturant compounds was evaluated. T...

In seeking alternatives for reducing environmental damage, fabricating filtration membranes using biopolymers derived from agro-industrial residues, such as cellulose acetate (CA), partially dissolved with green solvents, represents an economical and sustainable option. However, dissolving CA in green solvents through mechanical agitation can take...

A feeding trial was carried out to evaluate the effects of the partial replacement of fishmeal (FM) with soybean meal (SBM) or Lactobacillus acidophilus-fermented soybean meal (FSBM) on growth, feed efficiency, body composition, and digestive enzyme activity in juvenile white snook, Centropomus viridis. Five experimental diets were formulated, repl...

A huge number of byproducts and wastes are derived from the processing of fishery resources, which, in the case of teleost fish, can represent up to 70% of the initial biomass. In industry, fishery wastes are destined to produce oil and fishmeal used to elaborate animal feeds. Nevertheless, biomass from fish wastes has an excellent potential to be...

The poultry industry generates significant waste, making it vital to explore eco-friendly technologies for repurposing chicken by-products such as chicken hearts and livers. Recent advancements in emerging technologies have enhanced the extraction of hydrolysates. Consequently, this study aimed to assess the effectiveness of ultrasound-assisted hyd...

The purpose of this research is to produce functional ingredients with high bioactive value. The objective of this study was to investigate the extraction of functional ingredients from chicken liver and heart by ultrasound-assisted enzymatic hydrolysis. A Box-Behnken design was employed to optimize process conditions, considering ultrasound treatm...

Large quantities of by-products are generated after processing of rose snapper (Lutjanus guttatus), such as viscera, head, tail, skin, and bones, which are considered a potential source of valuable molecules. Therefore, the aim of the present study was the biochemical characterization of alkaline proteases isolated from the intestines of L. guttatu...

This study evaluated the effects on the growth performance and physiological response (metabolism, hematology, and antioxidant capacity) of the juvenile hybrid red tilapia Oreochromis sp. (initial average weight of 2.82 ± 0.02 g) cultured in seawater with biofloc and fed commercial diets containing 25, 35 and 40% crude protein (P25, P35, and P40, r...

BACKGROUND Soybean meal (SBM) is used widely in animal feed but it contains anti‐nutritional factors (ANFs) such as protease inhibitors – immunogenic proteins that limit its utilization. Fermentative processes could help to reduce these ANFs. The aim of this study was to evaluate the nutritional attributes, bacterial community dynamics, and microbi...

Currently, the mismanagement of marine by-products (head, skin, guts, blood, and bones) is a problem for the seafood processing industry, causing economic losses and environmental problems. Shrimp is a high-protein marine plankton species, and after processing, 45% of the total weight is discarded. Therefore, processes must be developed to recover...

Bioestimulantes de subproductos pesqueros con potencial agrícola.

Protein concentrates obtained from discarded grain flours of white chickpea Sinaloa (Cicer arietinum) (CC), “Azufrazin” bean (Phaseolus vulgaris) (BC), and white corn (Zea mays) (MC), were characterized biochemically through bromatological analyses (protein, lipid, fiber, moisture, ashes, and nitrogen free extract), HPLC techniques (amino acids con...

The objective of this research was to determine the effect of adding a mixture of different exogenous enzymes at 0.005% (0.0025% for each enzyme; protease+xylanase [P/X], protease+cellulase [P/C], and xylanase+cellulase [X/C]) to diets, based on fishmeal (FM) or soybean paste (SP), on shrimp's growth performance, survival, feed conversion, digestib...

This volume is a complete review of the cutting-edge scientific evidence about the isolation, identification, bioactivity and molecular analysis of the biologically active peptides (BAPs) obtained from several underutilized grains. It provides a general review of current and new technologies in isolating and bioprospecting BAPs before going into th...

The dairy industry traditionally uses aspartic proteases from bovine and microbial sources for cheese processing, but there is interest in alternative enzyme sources to supply the large global demand for rennet. This study investigated the biochemical characterization of a partially purified pepsin of yellowfin tuna with milk-clotting activity (MCA...

Amaranth sprouts contain phytochemicals with antioxidant activity that can neutralize free radicals that damage cellular macromolecules and change the stability of cells. In some seeds, phytochemicals content can be enhanced when these are imbibed with an aqueous seaweed extract (ASE). This study evaluated the effect of different concentrations (0....

Protein hydrolysates (PH) bioestimulants are an emerging technology that possess the ability to improve physiological processes such as germination and productivity yield in crops of agronomical importance. The bioestimulant potential on the germination of chickpea by two tilapia-byproducts PH at different degree of hydrolysis (DH) was studied. A f...

This paper aimed to evaluate the effects of feeding rate and the hormonal treatments for spawning induction on the condition factor, and the reproductive performance of the striped catfish, Pangasianodon hypophthalmus. A commercial diet with 35% of protein in three feeding rates-1%, 2%, and 3% biomass, were supplied for 184 days-and two hormonal me...

The Nile tilapia (Oreochromis niloticus) industry represent the 71% of aquaculture total world production and demands for novel feeds and better proteins. In this sense, Pacific thread herring (Ophistonema libertate) contains high-quality proteins suitable to produce protein hydrolysates (PH) with antioxidant activity for tilapia feeds. In this stu...

The effect of diets with varying crude protein-to-precooked cornstarch (P:S) ratios on growth performance, feed efficiency, carbohydrase activity, and blood chemistry of juvenile Lutjanus guttatus was evaluated. Four isoenergetic (19.0 kJ g-1) diets containing different P:S ratios (46:18, 38:30, 34:35, and 31:40) were formulated to feed fish for te...

La industria procesadora de productos pesqueros, derivado de sus actividades productivas, genera una importante cantidad de residuos líquidos, los cuales son comúnmente conocidos como efluentes pesqueros. Muchos de estos efluentes, son descargados al medio ambiente sin recibir algún tratamiento, generando un impacto negativo en los cuerpos de agua...

PurposeHigh amounts of rich-protein liquid wastes are produced during seafood processing. The effluent called stickwater resulting from the processing of Pacific thread herring (Ophistonema libertate) into fishmeal, was evaluated as protein source to produce bioactive protein hydrolysates by using Alcalase as enzyme source.Methods The effect of deg...

Seafood is regarded as a rich source of high-quality protein and nutraceuticals. Bioactive peptides are a distinctive example of chemicals that can improve the physiological condition of organisms, due to their wide range of biological properties, such as antioxidant, antimicrobial, antihypertensive, antiproliferative, etc. This chapter discusses t...

Revista Deveras- revista de ciencia para niños, en su número titulado "Planta con ciencia" se invita a las niñas y niños a danzar con el sol y los girasoles, también a adoptar un árbol; y a conocer al Chicozapote. Descarga el número gratis a través de la liga (https://comecyt.edomex.gob.mx/deveras-revista-ciencia).

Fish industry by-products can be a source of valuable compounds to apply in agricultural practices. Protein silage of heads, skin, fins, backbones and viscera of Nile tilapia (Oreochromis niloticus) was evaluated as stimulant on the growth of bread wheat sprouts. The tilapia acidic silage showed a lower content of crude lipids (13.5%) and higher co...

Protein hydrolysates from fishery byproducts have resulted to be nutraceutical ingredients with potential to be applied in human nutrition; however, critical quality attributes are dependent on some process parameters such as enzyme source and degree of hydrolysis. This study analyzed the biochemical properties and in vitro antioxidant activity (us...

Pacific red snapper (Lutjanus peru) is an important commercial species in Mexico with great aquaculture potential; however, digestive physiology is still unknown. Therefore, the objective of the present work was to characterize the digestive proteases of L. peru juvenile using biochemical and electrophoretic techniques. Results showed a higher acid...

This study aimed to assess the growth performance, antioxidant and immune responses of red tilapia (Oreochromis sp.) reared in seawater with two stocking densities (D1, 120 fish/m3; D2, 240 fish/m3) with biofloc plus probiotics. For each density, juvenile red tilapia (6.7 ± 0.2 g) were cultured under three different experimental conditions: clear s...

Purpose High amounts of rich-protein liquid wastes are produced during seafood processing. The effluent called stickwater resulting from the processing of Pacific thread herring (Ophistonema libertate) into fishmeal, was evaluated as protein source to produce bioactive protein hydrolysates by using Alcalase as enzyme source. Methods The effect of...

Large volumes of liquid waste are generated in processing operations of seafood industry. Such effluents have high concentrations of soluble protein, but it is necessary to concentrate it for utilization. Therefore, the present study investigated the effects of the use of hydrochloric (HCl) and trichloroacetic (TCA) acids on biochemical and antioxi...

Protein hydrolysates from fishery by-products, have resulted to be nutraceutical ingredients with potential to be applied in human nutrition; however, critical quality attributes are dependent of some process parameters such as enzyme source and degree of hydrolysis. This study reports the biochemical properties and antioxidant activity of protein...

In the present study, we evaluated new sources of plant proteases from fruits of Bromelia karatas (BK) and Bromelia pinguin (BP) to obtain antioxidant hydrolyzates/bioactive peptides (BPs) derived from chicken by-products (CH) and fish by-products (FH). The profile of the peptides was identified by reverse-phase high-resolution liquid chromatograph...

The fruit, vegetable, legume, and cereal industries generate many wastes, representing an environmental pollution problem. However, these wastes are a rich source of antioxidant molecules such as terpenes, phenolic compounds, phytosterols, and bioactive peptides with potential applications mainly in the food and pharmaceutical industries, and they...

The fruit, vegetable, legume, and cereal industries generate many wastes, representing an environmental pollution problem. However, these wastes are a rich source of antioxidant molecules such as terpenes, phenolic compounds, phytosterols, and bioactive peptides with potential applications mainly in the food and pharmaceutical industries, and they...

The recovery of proteases from fish viscera could be a strategy to reduce environmental problems caused by inadequate disposal of fish by-products. This study reports the biochemical characterization of proteases isolated from chihuil sea catfish (Bagre panamensis) intestines and the evaluation of their stability to different physical and chemical...

The replacement of fish meal (FM) by corn gluten meal (CGM) in practical diets for juvenile spotted rose snapper, Lutjanus guttatus, was evaluated. Six isonitrogenous (47% crude protein), isoenergetic (19.03 kJ g−1) diets were formulated by replacing 0, 20, 40, 60, 80, or 100% of FM protein with CGM protein. Diets with CGM inclusion were supplement...

A feeding trial was conducted to evaluate the effect of the partial replacement of fish meal (FM) by either soybean meal (SBM) or soybean protein concentrate (SPC) on growth, feed utilization, apparent digestibility (AD), and digestive capacity in the white snook, Centropomus viridis. Seven experimental diets were formulated by replacing 0, 15, 30,...

Aqueous seaweed extracts have diverse compounds such as Plant-Growth Regulators (PGRs) which have been utilized in agricultural practices for increasing crop productivity. Algal biomass of Padina durvillaei and Ulva lactuca have been suggested for use as biofertilizers because of plant growth-enhancing properties. This work aimed to identify the ma...

Sustainable aquaculture of the green guapote Mayaheros beani requires better understanding their physiology and composition. In that context, this research focused on the study of hematology, proximal and fatty acid (FA) composition of wild and farmed green guapote. Results showed no significant differences between wild and farmed fish in red blood...

Our research focused on the study of hematology, proximal and fatty acid (FA) composition of wild and farmed green guapote. Results showed no significant differences between wild and farmed fish in red blood cell count (1.77-2.57 ×106 cel μL-1), hemoglobin (11.6-15.1 g dL-1), glucose (68-115 mg dL-1) and the crude protein of whole body (51-58%). Ho...

Enormous amounts of by-products and waste are generated during the processing of seafood, sometimes representing about 65% of the raw material employed. Seafood by-products (SB) include solid waste such as skin, head, viscera, trimmings, and bones; however, liquid wastes (effluents) derived from operations such as washing, thawing, cooking and the...

Silicon dioxide has been used in aquaculture to increase the abundance of diatoms; however, its relationship with water quality or productive performance has not been investigated. Our study evaluated the effect of diatomite on water quality, growth performance and proximate composition of Penaeus vannamei reared in seawater (E1) and brackish water...

Pacific thread herring (Ophistonema libertate) muscle was hydrolyzed with Alcalase for the preparation of protein hydrolysates. The effect of enzyme concentration (EC; 1% and 3%), pH (8 and 9) and temperature (40°C and 50°C) on some biochemical properties and antioxidant activity (AOXA) was determined. The degree of hydrolysis (DH) ranged between 9...

One way to know the health condition of cultured organisms is through application of hematological tools. An experiment was carried out with juveniles of red tilapia Oreochromis spp. reared in sea water at two densities in clear water (114 fish/m3 and 228 fish/m3) or with biofloc (B1 and B2, respectively) and probiotic (B+P1 and B+P2). At the end o...

Pepsin from stomach of Bagre panamensis was semi-purified and biochemically characterized. The acid proteolytic activity and purification fold were 3875 U/mg protein and 91.85, respectively, after purification process. The optimum pH and temperature for semi-purified protease were 2–3 and 65 °C, respectively. The enzyme activity was stable after he...

Tilapia is one of the most cultivated fish in the world, but the low availability of freshwater for aquaculture is one of the main problems for its expansion. Although its cultivation in salt water and biofloc could be viable alternatives, scientific studies should to be done to know their physiology adaptation at different conditions. Our main goa...

The objectives of the present study were to characterize biochemically the proteases present in gastric juices from adults of the southern king crab (Lithodes santolla) and the Chilean rock crab (Cancer edwardsii), to determine the classes of proteases using specific inhibitors, as well as to evaluate the effect of temperature and pH that affect th...

Two formulated diets were evaluated to replace live crab (C) as feed for juveniles Enteroctopus megalocyathus. Formulated diets consisted of crab paste (CP) and a mixture of freeze-dried meals of crab and squid (C&S). After 10 weeks of feeding, the effect of each diet was analysed on productive performance, nutritional and physiological condition a...

Lipolytic activity from pancreatic crude extracts from the Pacific bluefin tuna (PBT), Thunnus orientalis; totoaba, Totoaba macdonaldi; and striped bass, Morone saxatilis, were biochemically characterized to detect possible differences in the digestive capacity of each fish. The maximum activity was at temperatures ranging from 35 to 45 C and at pH...

Se presentaron avances de la caracterización bioquímica de la actividad proteolítica presente en extractos digestivos de juveniles de Cichlasoma beani. Se evaluó el efecto de la temperatura, el pH e inhibidores enzimáticos en la actividad proteolítica de organismos mantenidos en cautiverio y silvestres.

The digestive process of the Pacific bluefin tuna (PBT), Thunnus orientalis, was simulated through two phases of in vitro digestion: acidic digestion with porcine pepsin, followed by alkaline digestion with pancreatic crude extract (PCE) obtained from the PBT to hydrolyze fish meal (FM) and soybean meal (SBM) as protein substrates. The crude protei...

The limited success reported in the paralarval culture of merobenthic octopus is mainly attributed to nutritional problems. This study aimed to determine the effect of live diets on the paralarvae performance during the first 30 days after hatching (DAH). The paralarvae were grown under different treatments: starved (STV) as negative control or fed...

In this study we assessed the influence of three different environmental salinities (5, 15 and 31 psu during 90 days) on growth, osmoregulation, energy metabolism and digestive capacity in juveniles of the Notothenioid fish Eleginops maclovinus. At the end of experimental time samples of plasma, liver, gill, intestine, kidney, skeletal muscle, stom...

One of the major problems involved in the controlled cultivation of Patagonian red octopus (En-teroctopus megalocyathus) is its long embryonic period ranging between 150–176 days, after which the hatching of planktonic paralarvae is achieved. The effect of temperature on the incubation of E. megalocyathus eggs was studied with the aim of establishi...

Morphological development and allometric growth patterns of Seriola lalandi larvae were assessed to characterize normal growth patterns under culture conditions. Early ontogenetic stages of yellowtail kingfish exhibited an exponential growth in terms of standard length as a function of age. Five development stages were characterized from hatching t...

The interaction between lysine (Lys) and arginine (Arg) in the proximal intestinal region of Pacific bluefin tuna (Thunnus orientalis) was evaluated using the everted intestine method. This in vitro intestinal system has been shown to be an effective tool for studying the nutrient absorption without the need to handle the tuna fish in marine cages...

Digestive capacity varies substantially in marine fish larvae during development, and the formulation of larval diets does not take this into account. The objectives of this study were to assess in vitro protein digestibility of dietary ingredients throughout the ontogeny of digestive system in California halibut, Paralichthys californicus, larvae...

The amino acid (AA) absorption along the intestinal tract of the Pacific bluefin tuna (Thunnus orientalis) was evaluated using two hydrolysed protein sources (fresh sardine muscle and soybean meal) with the everted intestine technique. Pork pepsin and pancreatic enzyme extract from the bluefin tuna were used to hydrolyse the protein from fresh sard...

The absorption rates of different amino acids (AAs) were compared using the everted intestine technique with fresh proximal intestinal sections from the Pacific bluefin tuna (Thunnus orientalis). Sardine muscle was hydrolyzed in two steps; first with porcine pepsin, followed by an alkaline pancreatic bluefin tuna extract. The hydrolyzed extract was...