Emma Teal

University of Cincinnati | UC · Department of Molecular and Cellular Physiology

Spasmolytic polypeptide/TFF2-expressing metaplasia is a mucous-secreting reparative lineage that emerges at the ulcer margin after in response to gastric injury. Under conditions of chronic inflammation with parietal cell loss, SPEM has been found to emerge and evolve into neoplasia. Cluster-of-differentiation gene 44 (CD44) is known to coordinate...

Background/Hypothesis Hedgehog (Hh) signaling not only plays a crucial role in embryonic development, but also controls gastric physiological events including epithelial cell differentiation, regeneration and acid secretion. Sonic Hedgehog (Shh), is secreted from gastric parietal cells in response to injury and contributes to the regeneration of th...

Cluster‐of‐differentiation gene 44, in particular CD44 variant isoforms (CD44v), emerges during regeneration of the gastric epithelium in response to injury. In particular, CD44v9 is expressed within Spasmolytic Polypeptide/TFF2‐Expressing Metaplasia (SPEM) glands during gastric repair, but the function is unknown. Here we tested the hypothesis tha...

Organoid cultures generated from gastrointestinal tissues have been an invaluable advancement for in vitro studies of physiological function and disease. Here we present a comprehensive protocol for the establishment and culture of human- and mouse-derived 3-dimensional gastric organoids transferred to 2-dimensional gastric epithelial cell monolaye...

In vitro studies of gastric wound repair typically involves the use of gastric cancer cell lines in a scratch-wound assay of cellular proliferation and migration. One critical limitation of such assays, however, is their homogenous assortment of cellular types. Repair is a complex process which demands the interaction of several cell types. Therefo...

Cluster-of-differentiation gene 44, in particular CD44 variant isoform 9 (CD44v9), emerges during regeneration of the gastric epithelium in response to injury. In particular, CD44v9 is expressed within Spasmolytic Polypeptide/TFF2-Expressing Metaplasia (SPEM) glands during gastric repair, but the function is unknown. Here we tested the hypothesis t...

Background: The Hedgehog (Hh) signaling pathway is often reactivated in various types of cancers including gastric, and hence raises the interest of targeting the Hh pathway as a potential therapeutic target for the treatment of gastric cancer. Tumor cells expressing programmed cell death 1 (PDL-1) interact with PD-1 on CD8+ cytotoxic T lymphocytes...