Emiel A.M. Janssen

Emiel A.M. Janssen
Stavanger University Hospital · Department of Pathology

Professor in biomedicine, University of Stavanger

About

239
Publications
26,185
Reads
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4,696
Citations
Additional affiliations
September 2019 - present
Griffith University
Position
  • Professor (Associate)
January 2015 - present
University of Stavanger (UiS)
Position
  • Professor
March 2004 - May 2007
University of Bergen
Position
  • PhD Student

Publications

Publications (239)
Article
1090 Background: Tumor recurrence and metastatic progression remain the leading cause for breast cancer related mortalities. The study aimed to identify differences in proteomics landscape in serum between (i) healthy controls and breast cancer patients, (ii) baseline samples at time of diagnosis from patients that later developed metastases versus...
Article
Full-text available
Background: Although triple-negative breast cancer (TNBC) is associated with an increased risk of recurrence and metastasis, the molecular mechanisms underlying metastasis in TNBC remain unknown. To identify transcriptional changes and genes regulating metastatic progression in TNBC, we compared the transcriptomic profiles of primary and matched m...
Article
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Background Matching treatment based on tumour molecular characteristics has revolutionized the treatment of some cancers and has given hope to many patients. Although personalized cancer care is an old concept, renewed attention has arisen due to recent advancements in cancer diagnostics including access to high-throughput sequencing of tumour tiss...
Article
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Introduction: Breast cancer is still the most common malignancy among women worldwide. The Prospective Breast Cancer Biobank (PBCB) collects blood and urine from patients with breast cancer every 6 or 12 months for 11 years from 2011 to 2030 at two university hospitals in Western Norway. The project aims to identify new biomarkers that enable dete...
Preprint
Background: Matching treatment based on tumour molecular characteristics has revolutionized the treatment of some cancers and has given hope to many patients. Although personalized cancer care is an old concept, renewed attention has arisen due to recent advancements in cancer diagnostics including access to high-throughput sequencing of tumour tis...
Article
Background: Pathological complete response (pCR) after neoadjuvant chemotherapy (NAC) isassociated with a favorable prognosis and low recurrence rate, particularly in patients with triple-negative breast cancer (TNBC), an aggressive breast cancer subtype. However, only 30%-40%of patients with TNBC show pCR after standard NAC, while the remaining pa...
Article
Background: Triple-negative breast cancer (TNBC) is a heterogeneous breast cancer subtype with distinct biological features and clinical behavior. TNBC is associated with an increased risk of metastasis and recurrence. In this whole exome study, we investigated the genetic profiles of primary TNBC tumors and paired metastases using next-generation...
Article
Full-text available
Implementation of high-risk human papilloma virus (HPV) screening and the increasing proportion of HPV vaccinated women in the screening program will reduce the percentage of HPV positive women with oncogenic potential. In search of more specific markers to identify women with high risk of cancer development, we used RNA sequencing to compare the t...
Article
Full-text available
Centrosome amplification (CA) has been implicated in the progression of various cancer types. Although studies have shown that overexpression of PLK4 promotes CA, the effect of tumor microenvironment on polo-like kinase 4 (PLK4) regulation is understudied. The aim of this study was to examine the role of hypoxia in promoting CA via PLK4. We found t...
Preprint
Full-text available
The recommended treatment for patients with high-risk non-muscle invasive bladder cancer (HR-NMIBC) is tumor resection followed by adjuvant Bacillus Calmette-Guérin (BCG) bladder instillations. However, only 50% of patients benefit from this therapy. In case of progression to advanced disease, patients must undergo a radical cystectomy with signifi...
Article
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The advent of immune-checkpoint inhibitors (ICI) in modern oncology has significantly improved survival in several cancer settings. A subgroup of women with breast cancer (BC) has immunogenic infiltration of lymphocytes with expression of programmed death-ligand 1 (PD-L1). These patients may potentially benefit from ICI targeting the programmed dea...
Article
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The purpose of this study was to establish a gene signature that may predict CIN3 regression and that may aid in selecting patients who may safely refrain from conization. Oncomine mRNA data including 398 immune-related genes from 21 lesions with confirmed regression and 28 with persistent CIN3 were compared. L1000 mRNA data from a cervical cancer...
Chapter
Full-text available
In triple negative breast cancer (TNBC) treatment, early prediction of pathological complete response (PCR) from chemotherapy before surgical operations is crucial for optimal treatment planning. We propose a novel deep learning-based system to predict PCR to neoadjuvant chemotherapy for TNBC patients with multi-stained histopathology images of ser...
Article
Full-text available
The most common type of bladder cancer is urothelial carcinoma, which is among the cancer types with the highest recurrence rate and lifetime treatment cost per patient. Diagnosed patients are stratified into risk groups, mainly based on grade and stage. However, it is well known that correct grading of bladder cancer suffers from intra-and interob...
Article
Full-text available
Obesity is an established risk factor for postmenopausal breast cancer and has been linked to worse breast cancer prognosis, most clearly for hormone receptor-positive breast cancers. The underlying mechanisms of the obesity–breast cancer association are not fully understood, but growing evidence points to the breast adipose tissue microenvironment...
Article
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Numerical and/or structural centrosome amplification (CA) is a hallmark of cancers that is often associated with the aberrant tumor karyotypes and poor clinical outcomes. Mechanistically, CA compromises mitotic fidelity and leads to chromosome instability (CIN), which underlies tumor initiation and progression. Recent technological advances in micr...
Article
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Different miRNA profiling protocols and technologies introduce differences in the resulting quantitative expression profiles. These include differences in the presence (and measurability) of certain miRNAs. We present and examine a method based on quantile normalization, Adjusted Quantile Normalization (AQuN), to combine miRNA expression data from...
Article
Full-text available
Background The antitumor efficacy of PARP inhibitors (PARPi) for breast cancer patients harboring germline BRCA1/2 (gBRCA1/2) mutations is well established. While PARPi monotherapy was ineffective in patients with metastatic triple negative breast cancer (TNBC) wild type for BRCA1/2, we hypothesized that PARPi may be effective in primary TNBCs with...
Conference Paper
Full-text available
Supervised learning of convolutional neural networks (CNN) used for image classification and segmentation has produced state-of-the-art results, including in many medical image applications. In the medical field, making ground truth labels would typically require an expert opinion , and a common problem is the lack of labeled data. Consequently, th...
Conference Paper
p> Background: The presence of supernumerary or abnormally large centrosomes, also known as centrosome amplification (CA), drives tumor progression by enhancing chromosomal instability, and altering cellular microtubule cytoskeletons and tissue cytoarchitectures to promote the migration and invasion capabilities of cancer cells. Although the preval...
Article
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Objective: To describe early experience of replacing PSA with Stockholm3 for detection of prostate cancer in primary care. Design and methods: Longitudinal observations, comparing outcome measures before and after the implementation of Stockholm3. Setting: Stavanger region in Norway with about 370,000 inhabitants, 304 general practitioners (GP...
Article
Full-text available
The Covid-19 pandemic represents an unsolved problem which has caused numerous fatalities. At the present time, there is no vaccination available or curative therapy. However, recent reports suggest that SARS-CoV2 acts upon its functional receptor ACE2 inducing a variety of deleterious effects such as inflammation, endothelial dysfunction, microang...
Conference Paper
Urothelial carcinoma is the most common type of carcinoma of the urinary bladder in the Western world. Currently TNM stage and WHO grade are the most important histopathologic parameters to classify patients into low- and high-risk groups and to decide appropriate treatment. However, as differentiating between stage Ta and T1 on surgical specimens...
Article
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Purpose: The proliferation marker Ki-67 has been used as a prognostic marker to separate low- and high-risk breast cancer subtypes and guide treatment decisions for adjuvant chemotherapy. The association of Ki-67 with response to tamoxifen therapy is unclear. High-throughput automated scoring of Ki-67 might enable standardization of quantification...
Preprint
Full-text available
Protein structural alterations, including misfolding and aggregation, are a hallmark of several diseases, including cancer. However, the possible clinical application of protein conformational analysis using infrared spectroscopy to detect cancer-associated structural changes in proteins has not been established yet. The present study investigates...
Article
Full-text available
In urothelial cell type non-muscle invasive urinary bladder carcinoma, TNM stage and WHO grade are widely used to classify patients into low and high‑risk groups for prognostic and therapeutic decision-making. However, stage and grade reproducibility and prediction accuracy are wanting. This may lead to suboptimal treatment. We evaluated whether pr...
Article
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Background: Previously, we have shown that miR-18a and miR-18b gene expression strongly correlates with high proliferation, oestrogen receptor -negativity (ER-), cytokeratin 5/6 positivity and basal-like features of breast cancer. Methods: We investigated the expression and localization of miR-18a and -18b in formalin fixed paraffin embedded (FF...
Article
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Epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 3 (HER3) have been investigated as triple-negative breast cancer (TNBC) biomarkers. Reduced EGFR levels can be compensated by increases in HER3; thus, assaying EGFR and HER3 together may improve prognostic value. In a multi-institutional cohort of 510 TNBC patients,...
Article
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Purpose: To predict the risk of local recurrence (LR) in ductal carcinoma in situ (DCIS) with a new visualization and quantification approach using centrosome amplification (CA), a cancer-cell specific trait, widely associated with aggressiveness. Experimental design: This first-of-its-kind methodology evaluates the severity and frequency of num...
Article
Full-text available
In pathology labs worldwide, we see an increasing number of tissue samples that need to be assessed without the same increase in the number of pathologists. Computational pathology, where digital scans of histological samples called whole-slide images (WSI) are processed by computational tools, can be of help for the pathologists and is gaining res...
Article
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Background: The metabolic consequences of preoperative carbohydrate load in breast cancer patients are not known. The present explorative study investigated the systemic and tumor metabolic changes after preoperative per-oral carbohydrate load and their influence on tumor characteristics and survival. Methods: The study setting was on university...
Article
Full-text available
Background: Operable breast cancer patients may experience late recurrences because of reactivation of dormant tumor cells within the bone marrow (BM). Identification of patients who would benefit from extended therapy is therefore needed. Methods: BM samples obtained pre- and post-surgery were previously analysed for presence of disseminated tu...
Article
Full-text available
Background: Conflicting results have been reported on the influence of carbohydrates in breast cancer. Objective: To determine the influence of pre-operative per-oral carbohydrate load on proliferation in breast tumors. Design: Randomized controlled trial. Setting: University hospital with primary and secondary care functions in South-West N...
Preprint
Background The influence of carbohydrates in breast cancer is conflicting.Objective To determine whether preoperative per-oral carbohydrate load influences proliferation in breast tumors.Design Randomized controlled trial.Setting University hospital with primary and secondary care functions in South-West Norway.Patients A population-based cohort of...
Article
Background Neoadjuvant treatment of triple negative breast cancer (TNBC) in general implies different chemotherapy regimens administered in sequence. PARP inhibitors like olaparib have revealed clinical benefit in TNBC harboring BRCA germline mutations, but were ineffective when tested as monotherapy for BRCA WT metastatic breast cancer. While comb...
Article
Background Optimal neoadjuvant therapy for luminal A breast cancer remains a topic of controversy. Methods In the phase 2 PETREMAC trial (NCT02624973), patients with large T2 (>4cm) or locally advanced breast cancers and luminal A characteristics (ER > 50%, HER2- and TP53 WT) received neoadjuvant endocrine therapy (NET) and CDK4/6 inhibition in co...
Preprint
Background The influence of carbohydrates in breast cancer is conflicting.Objective To determine whether preoperative per-oral carbohydrate load influences proliferation in breast tumors.Design Randomized controlled trial.Setting University hospital with primary and secondary care functions in South-West Norway.Patients A population-based cohort of...
Preprint
Background: The metabolic consequences of preoperative carbohydrate load in breast cancer patients are not known. The present study investigated the systemic and tumor metabolic changes after preoperative per-oral carbohydrate load and their influence on tumor characteristics and survival. Design: Explorative study. Setting: University hospital wi...
Article
Full-text available
The efforts to personalize treatment for patients with breast cancer have led to a focus on the deeper characterization of genotypic and phenotypic heterogeneity among breast cancers. Traditional pathology utilizes microscopy to profile the morphologic features and organizational architecture of tumor tissue for predicting the course of disease, an...
Preprint
Full-text available
Correct treatment of urothelial carcinoma patients is dependent on accurate grading and staging of the cancer tumour. This is determined manually by a pathologist by examining the histological whole-slide images (WSI). The large size of these images makes this a time-consuming and challenging task. The WSI contain a variety of tissue types, and a m...
Article
Full-text available
Background: European treatment guidelines for pTa and pT1 urinary bladder urothelial carcinoma depend highly on stage and WHO-grade. Both the WHO73 and the WHO04 grading systems show some intra- and interobserver variability. The current pilot study investigates which histopathological features are especially sensitive for this undesired lack of r...
Article
Full-text available
Purpose Tamoxifen is an important targeted endocrine therapy in breast cancer. However, side effects and early discontinuation of tamoxifen remains a barrier for obtaining the improved outcome benefits of long-term tamoxifen treatment. Biomarkers predictive of tamoxifen side effects remain unidentified. The objective of this prospective population-...
Article
Full-text available
Background: The androgen receptor (AR) has emerged as a potential therapeutic target for AR-positive triple-negative breast cancer (TNBC). However, conflicting reports regarding AR's prognostic role in TNBC are putting its usefulness in question. Some studies conclude that AR positivity indicates a good prognosis in TNBC, whereas others suggest th...
Preprint
Background The influence of carbohydrates in breast cancer is conflicting. Objective To determine whether preoperative per-oral carbohydrate load influences proliferation in breast tumors. Design Randomized controlled trial. Setting University hospital with primary and secondary care functions in South-West Norway. Patients A population-based cohor...
Article
Full-text available
Malignant melanoma is a severe and aggressive type of skin cancer, with a rapid decrease in survival rate if not diagnosed and treated at an early stage. Histopathological examination of hematoxylin and eosin stained tissue biopsies under a light microscope is currently the gold standard for diagnosis. However, this manual examination is a difficul...
Article
Full-text available
Protein expression of Myristoylated alanine-rich C kinase substrate like-1 (MARCKSL1) has been identified as a prognostic factor in lymph-node negative (LN⁻) breast cancer patients. We aim to validate MARCKSL1 protein expression as a prognostic marker for distant metastasis-free survival (DMFS) in a new cohort of LN⁻ breast cancer patients. MARCKSL...
Data
Comparison of the discovery cohort and the validation cohort. (PDF)
Data
MARCKSL1 expression scores in a) validation cohort and b) discovery cohort (Jonsdottir et al. 2012). (PDF)
Data
Example of MARCKSL1 (myristoylated alanine-rich C kinase substrate like-1) staining (brown staining). A) Strong membrane staining. B) Strong granular staining. C) Strong cytoplasmic staining. D) Negative/Weak staining. (PDF)
Poster
Background: About 60-80% of ductal carcinoma in situ (DCIS) cases are high-grade (HG) DCIS with an elevated risk of local recurrence (LR) even after a lumpectomy. Patients are often under or over treated due to the lack of accurate recurrence risk prediction models. Current prognostic models such as OncotypeDX and Van Nuys Prognostic Index (VNPI) l...
Article
Background and rationale: Side effects of adjuvant treatment with tamoxifen (tam) may impair Quality of Life (QoL) and have been suggested as an independent variable for discontinuation of tam¹. There are large inter-patient variabilities in prevalence and severance of side effects among tam users. Therefore, there is a need for biological markers...