Elsayed Metwllay

Elsayed Metwllay
Suez Canal University · Department of Cytology and Histology

Doctor of Veterinary Medicine
Since my early years as graduate student, I have been very interested in neouronal remodling and neuronal injury.

About

24
Publications
1,742
Reads
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73
Citations
Introduction
During my Ph.D. training, I focused on uncovering the molecular mechanisms regulating glutamate receptor (GluR) expression at synapses. I then identified from a candidate screen that Ttm50 act as calpain activator. After extensive training in Zhang Laboratory, I am familiar to various techniques such as cell culture, gene amplification, histology, biochemistry, animal handling.
Additional affiliations
September 2013 - August 2017
Institute of Genetics and Developmental Biology, CAS
Position
  • Ph.D. (Neurobiology and Developmental Biology), China, 2013-2017
Description
  • During my Ph.D. training, I focused on uncovering the molecular mechanisms regulating glutamate receptor (GluR) expression at synapses. From an RNAi screen using the abundance of GluRIIA at peripheral synapses as a readout, I found that the calpain proteinase negatively and specifically regulates the synaptic and total protein levels of the glutamate receptor GluRIIA in a calcium-dependent manner (Metwally et al., Journal of Neuroscience, 2019. DOI: 10.1523/jneurosci.2213-17.2019).
Education
December 2009 - December 2013
Suez Canal University Faculty of veterinary medicine
Field of study
  • Histology

Publications

Publications (24)
Article
In both veterinary and human health, regenerative medicine offers a promising cure for various disorders. One of the rate‐limiting challenges in regenerative medicine is the considerable time and technique required to expand and grow cells in culture. Therefore, the stromal vascular fraction (SVF) shows a significant promise for various cell therap...
Article
Full-text available
In veterinary practice, numerous urological disorders that cause bladder dysfunction necessitate augmentation cystoplasty (AC). The purpose of this study is to evaluate the dog tunica vaginalis allograft (DTVA), sheep tunica vaginalis xenograft (STVX) and sheep tunica vaginalis decellularized extracellular matrix (STVDEM) as graft materials for uri...
Article
Full-text available
It has been widely known that oxidative stress disrupts the balance between reactive oxygen species (ROS) and the antioxidant system in the body. During pregnancy, the physiological generation of ROS is involved in a variety of developmental processes ranging from oocyte maturation to luteolysis and embryo implantation. While abnormal overproductio...
Poster
Full-text available
Calpains are evolutionarily conserved and widely expressed Ca 2+-activated cysteine proteases that act at neutral pH. The activity of calpains is tightly regulated, given that their abnormal activation can have deleterious effects leading to promiscuous cleavage of various targets. Genetic mutations in the genes encoding calpains are associated wit...
Article
Full-text available
Mesenchymal stem cells (MSCs) derived from adipose tissue are evolved into various cell-based regenerative approaches. Adipose-derived stem cells (ASCs) isolated from rats are commonly used in tissue engineering studies. Still, there is a gap in knowledge about how the harvest locations influence and guide cell differentiation. This study aims to i...
Article
Full-text available
Gut microbiota is the natural residents of the intestinal ecosystem which display multiple functions that provide beneficial effects on host physiology. Disturbances in gut microbiota in weaning stress are regulated by the immune system and oxidative stress-related protein pathways. Weaning stress also alters gut microbiota response, limits digesti...
Article
Calpains are evolutionarily conserved and widely expressed Ca²⁺-activated cysteine proteases that act at neutral pH. The activity of calpains is tightly regulated, given that their abnormal activation can have deleterious effects leading to promiscuous cleavage of various targets. Genetic mutations in the genes encoding calpains are associated with...
Article
Full-text available
Mitochondria are involved in a variety of developmental processes and neurodegenerative diseases. The translocase complexes of the outer and inner mitochondrial membranes (TOM and TIM) are protein complexes involved in transporting protein precursors across mitochondrial membranes. Although rabbits are important animal models for neurodegenerative...
Article
Full-text available
Abstract It has been well recognized that interactions between the gut microbiota and host-metabolism have a proven effect on health. The gut lumen is known for harboring different bacterial communities. Microbial by-products and structural components, which are derived through the gut microbiota, generate a signaling response to maintain homeostas...
Article
Full-text available
Defective implantation is related to pregnancy-associated disorders such as spontaneous miscarriage, intrauterine fetal growth restriction and others. Several factors proclaimed to be involved such as physiological, nutritional, environmental and managemental that leads to cause oxidative stress. Overloading of free radicals promotes oxidative stre...
Article
Full-text available
The balance among different subtypes of glutamate receptors (GluRs) is crucial for synaptic function and plasticity at excitatory synapses. However, the mechanisms balancing synaptic GluR subtypes remain unclear. Herein, we show that the two subtypes of GluRs A and B expressed at Drosophila neuromuscular junction (NMJ) synapses mutually antagonize...
Article
Full-text available
The balance among different subtypes of glutamate receptors (GluRs) is crucial for synaptic function and plasticity at excitatory synapses. However, the mechanisms balancing synaptic GluR subtypes remain unclear. Herein, we show that the two subtypes of GluRs A and B expressed at Drosophila neuromuscular junction (NMJ) synapses mutually antagonize...
Article
Calcium-dependent proteolytic calpains are implicated in a variety of physiological processes, as well as pathologies associated with calcium overload. However, the mechanism by which calpain is activated remains elusive since intracellular calcium levels under physiological conditions do not reach the high concentration range required to trigger c...
Presentation
Calpains are a family of cytosolic cysteine proteinases that are implicated in a variety of biological processes, as well as injury and neurodegeneration associated with ischemia and Alzheimer’s disease. Calpain proteolytic activity depends on calcium, but the mechanisms whereby this activity is regulated remains elusive. In our previous study, we...
Article
Effect of dietary supplementation with a synbiotic (Lacto Forte) on growth performance, haematological and histological profiles, the innate immune response and resistance to bacterial disease in Oreochromis niloticus
Article
Full-text available
Calpains are calcium-dependent, cytosolic proteinases active at neutral pH. They do not degrade but cleave substrates at limited sites. Calpains are implicated in various pathologies, such as ischemia, injuries, muscular dystrophy, and neurodegeneration. Despite so, the physiologicalfunction of calpainsremainsto be clearly defined.Usingthe neuromus...
Poster
Full-text available
Calpains are cytosolic proteinases that do not degrade but cleave substrates at limited sites. They are implicated in various developmental processes and pathologies such as ischemia, muscular dystrophy, and neurodegeneration. Calpain activity depends on calcium, but the mechanisms whereby this activity is regulated remains elusive. Using the Droso...
Article
Full-text available
The optic nerve (ON) is an important organ of the animal visual system, which carries transfers electrical impulses toward the brain from the retina to the brain. As we all know, the high enrichment of glial cells in optic nerve is known to supports neuron and regulate retinal homeostasis. However, research on the immunohistochemical intensity of d...
Presentation
Calpains are cytosolic proteinases that do not degrade but cleave substrates at limited sites. They are implicated in various developmental processes and pathologies such as ischemia, muscular dystrophy, and neurodegeneration. Calpain activity depends on calcium, but the mechanisms whereby this activity is regulated remains elusive. Using the Droso...
Poster
Full-text available
Vast majority of excitatory neurotransmission in mammalian central nervous system (CNS) is mediated by glutamatergic synapses. Glutamate receptors (GluRs) in Drosophila neuromuscular synapses are conserved with mammalian non-NMDA receptors. So far, we know little about regulation of different GluR subtypes. There are two GluR subtypes in the Drosop...
Presentation
Calpains are calcium-dependent, cytosolic proteinases active at neutral pH. They do not degrade but cleave substrates at limited sites. Calpains are implicated in various pathologies, such as ischemia, injuries, muscular dystrophy, and neurodegeneration. Despite so, the physiological function of calpains remains to be clearly defined. Using the neu...
Poster
Full-text available
Calpains are cytosolic proteinases that do not degrade but cleave substrates at limited sites. They are implicated in various developmental processes and pathologies such as ischemia, muscular dystrophy, and neurodegeneration. Calpain activity depends on calcium, but the mechanisms whereby this activity is regulated remains elusive. Using the Droso...
Poster
Full-text available
The efficiency of neurotransmission is determined by the level of neurotransmitter receptors at the postsynaptic densities. However, the molecular mechanism by which the level of neurotransmitter receptor expression at synapses is regulated is poorly understood. From a genetic screen, we found that the protein level of glutamate receptor IIA (GluRI...

Questions

Questions (2)
Question
I want to construct one plasmid to express in S2 cells. I'm asking the best additional base-pair to be added before the Acc651 enzyme
Question
Trying to construct protein with 6xMyc tag, but found the expressed band is little higher than expected by 10 kDa. is the reason for this the long spacer between Myc tag?.

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Projects

Projects (5)
Project
The main objective of this study is to examine various types of grafts, particularly natural low-cost ones, for use in soft tissue surgery on pets. To assist veterinarians in determining appropriate graft material in a variety of clinic situations.
Project
Dissenting the Molecular Mechanisms Controlling GluRs Balance at Synapses at the Basis of structure, translocation and organization.