Elsa Helena Walter de Santana

Elsa Helena Walter de Santana
University of Northern Parana · Master's degree in Animal Health and Production

PhD Animal Science

About

94
Publications
24,661
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399
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Introduction
Graduated in Veterinary Medicine from State University of Londrina (1999), Master's degree in Animal Health (2001), and Ph.D. in Animal Science (2006) from State University of Londrina. Professor since 2005 at University of Northern Parana(Unopar) in the veterinary medicine course, in the areas of inspection and technology of animal products. Professor of the Programs Animal Health and Production (UNOPAR) and Animal Bioscience (UNIC).
Additional affiliations
January 2008 - August 2017
University of Northern Parana
Position
  • Professor

Publications

Publications (94)
Article
In this research communication the genetic diversity of Pseudomonas fluorescens ( n = 67) and Pseudomonas putida ( n = 44) isolated from refrigerated raw milk from bulk tank trucks were verified. The relationship between the genetic profile of the isolates and their lipoproteolytic potential was evaluated using skim milk agar and tributyrin agar (2...
Article
This study reports the effect of psychrotrophs in raw milk on the quality of Mozzarella cheese. Physical, chemical, sensorial and textural characteristics were evaluated, and the indices of lipolysis and proteolysis were assessed. The highest population (7 log cfu/mL vs. 3 log cfu/mL) was associated with a decrease in yield and in primary proteolys...
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This research paper aimed to optimize the process parameters and to select the most suitable drying method in order to obtain probiotic powder at reduced cost. The influence of the addition of growth promoters (glucose, sucrose, cheese whey, peptone, yeast extract or ammonium sulfate), incubation parameters (time and temperature) and drying method...
Article
The aim of the present study was to evaluate the physical-chemical profile of raw sheep milk and the influence of storage temperature on the population of microorganisms. Sheep milk samples were collected from the milk cans (500 mL/sample) and were evaluated the physical-chemical composition: fat, protein, lactose, ash content, total dry matter (TD...
Article
Produtos lácteos fermentados contêm bactérias ácido lácticas (BAL), naturalmente presentes ou adicionadas na matriz láctea como culturas iniciadoras (starters), contribuindo com aroma, textura, valor nutricional e segurança microbiológica. Lactobacillus spp., Streptococcus spp., Lactococcus spp. e Leuconostoc spp. são utilizados como culturas start...
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Psychrotrophic microorganisms, especially Pseudomonas spp., are present in the microbiota of refrigerated milk as they can grow at refrigeration temperatures irrespective of their optimal growth temperature. Psychrotrophic counts ranging from 105 to 108 CFU/mL in refrigerated raw milk effect cheese quality, since the synthesized thermoresistant enz...
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This study aimed to determine the frequency of Pseudomonas fluorescens, P. putida, and P. aeruginosa in refrigerated raw milk; their proteolytic potential; and your association with the aprX gene. Of the 173 isolates confirmed as belonging to Pseudomonas spp., 37% were P. fluorescens, 25.4% P. putida, and none belongs to P. aeruginosa. The aprX gen...
Article
Background: Provolone can be fresh or ripened, and its taste varies from sweet to spicy. The high psychrotrophic density of raw milk is associated with thermo-resistant enzymes that can change cheese characteristics such as texture, promote sensory defects, and decrease industrial yield. Two batches of provolone-type smoked cheese were produced fr...
Article
The objective of the work described in this research communication was to determine the lipoproteolytic capacity and potential of Pseudomonas spp. from the microbiota of refrigerated raw milk. The influence of temperature and bacterial population on these activities was also evaluated. Pseudomonas spp. (PS) counts (30 °C/48 h) were confirmed by PCR...
Article
Background: The selection of the food matrix to be used as a vehicle for a probiotic culture is important, because its chemical composition and physicochemical characteristics can affect the probiotic survival during the shelf-life of the product and on simulated gastrointestinal conditions (SGIC). This study aimed to evaluate the influence of the...
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This work aimed to assess the inhibitory effect of Lactobacillus rhamnosus against different populations of Pseudomonas fluorescens and Pseudomonas putida, isolated from refrigerated raw milk and the compounds responsible for inhibitory effects over storage time. An initial population of 4 log cfu/ml (50 ml) of L. rhamnosus (working culture) was ad...
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The aim of this study was to evaluate the lipolytic index (LI) of Pseudomonas fluorescens and Pseudomonas putida (2, 5, 6 log CFU/mL) in milk during 96 h by the Lipo R method. The strains were isolated from refrigerated raw milk (30 °C, 48 h), and species were confirmed by PCR, inoculated in reconstituted whole milk, and stored at 2 °C, 4 °C, and 8...
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The effects of weather/season (winter and summer), milk production (high and low), and milking time (night and day) on melatonin levels were evaluated. The melatonin in the milk from 30 individual cows, the bulk tank milk (BTM) from 16 farms, and ultra-high temperature (UHT) processed milk of 12 brands over two seasons were also assessed. The melat...
Article
BACKGROUND Aflatoxin M1 (AFM1) is a mycotoxin that can be found in milk as a result of the ingestion of aflatoxin B1 (AFB1) by dairy cattle. Due to its carcinogenic properties, the control of its occurrence in dairy products is of great importance. We evaluated the occurrence and seasonality of AFM1 in milk from farms with subtropical and temperate...
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This Research Communication describes the relationship between casein, free fatty acids (FFAs) and the storage period of ultra-high temperature-treated (UHT) whole milk observed for a period of 120 d of labelled shelf-life. Moreover, we aim to estimate the daily rate of casein degradation in UHT whole milk, and the total length of time estimated fo...
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The objective of the work was to evaluate the multiplication capacity and proteolytic activity of different Pseudomonas spp. cell counts inoculated in milk and storaged under different temperature. Strains isolated from refrigerated raw milk (RRM) were confirmed at genus level by Polymerase Chain Reaction (PCR). The Pseudomonas spp. was cultured in...
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Background: High-performance liquid chromatography (HPLC) is widely employed to determine the caseinomacropeptide (CMP) index and detect milk tampering with rennet whey. Prior to HPLC analysis, CMP is subject to a trichloracetic acid (TCA) isolation causing further soluble proteins in the sample to precipitate. On this basis, we wanted to estimate...
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A conservação do leite cru sob refrigeração favorece o crescimento de psicrotróficos, produtores de enzimas proteolíticas e lipolíticas termorresistentes. Pseudomonas spp e P. fluorescens são relatados como os psicrotróficos de maior frequência em leite refrigerado. O objetivo foi avaliar a população de mesófilos, psicrotróficos e Pseudomonas spp....
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Thresholds for Somatic Cell Counts (SCC) and Total Bacterial Counts (TBC) in refrigerated raw milk have been stricter in Brazil since July 2014. We evaluated whether the composition of 11,051 milk samples delivered to processing plants in Paraná state, Brazil, by cooperative dairy farms, complies with government requirements and established changes...
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The psychrotrophic bacteria growth in refrigerated milk is responsible for the production of heat resistant enzymes, including those which have lipolytic capacity. Lipases can cause rancidity and off-flavor in pasteurized and UHT milk, milk powder and cheese. Pseudomonas spp. is the major genera responsible by lipases synthesis, mainly Pseudomonas...
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Raw milk samples were collected from cooling tanks (after they cooled for 48 h) in five dairy farms and the corresponding bulk tank (bulk milk transportation, BMT) when they arrived to the industry. Routine physical chemical analyzes and quantification of psychrotrophic (Pseudomonas spp. and P. fluorescens) and aerobic mesophilic (AM) populations w...
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The use of efficient methods for the early diagnosis of subclinical mastitis is of great importance in milk production. By using an electrical conductivity meter (EC), the efficiency of subclinical mastitis diagnosis was evaluated in dairy farms in northern Parana, Brazil, and the results were compared with those obtained by using the California Ma...
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This study aimed at developing a flan-type dairy dessert supplemented with Lactobacillus acidophilus La-5 and whey protein concentrate (WPC). Four flan-type dairy desserts containing L. acidophilus La-5 were produced: F1 (0% WPC), F2 (1.5% WPC), F3 (3% WPC) and F4 (4.5% WPC). Instrumental texture, physicochemical and microbiological analyses were d...
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The viability of Lactobacillus acidophilus La-5 incorporated into ice cream produced with and without hydrolysis of lactose was evaluated. Furthermore, the effects of La-5 and lactose hydrolysis on the physicochemical characteristics of the final product were analyzed. Three formulations were produced: T1 (without La-5 and lactose hydrolysis); T2 (...
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The main components in milk from cows that are affected by mastitis can be in different amounts according to the racial characteristics. Thus, the association between the somatic cell counts (SCC) and these components may also differ in milk from Jersey cows. This study evaluated the effect of the SCC in milk from Jersey cows on milk production and...
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Over the past few years, the dairy sheep industry have been taking space in the Brazilian national and international market. In this way, this work aimed to design a delineation of the Brazilian dairy sheep industry, with focus on aspects of the legislation, the composition and quality of milk. The explore shows up quite advantageous and profitable...
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Full-text available
The main components in milk from cows that are affected by mastitis can be in different amounts according to the racial characteristics. Thus, the association between the somatic cell counts (SCC) and these components may also differ in milk from Jersey cows. This study evaluated the effect of the SCC in milk from Jersey cows on milk production and...
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p>The aim of this research was to identify the occurrence of pathogens causing subclinical mastitis in grade B milk farms of the Jaguapitã county, state of Paraná, Brazil. California Mastitis Test (CMT) were carried out in 400 milk samples from 100 animals and 157 teats from 55 animals (55%) were positive, showed score two or higher to CMT. When th...
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The aims of present study were to evaluate the effects of milk somatic cell count (SCC) and heat stress on yield and milk composition of cows in a herd for commercial production in a temperate region during the period 2008-2012. Data from the monthly milk test-day records of 161±9 Holstein, totaling 9,650 milkings, were provided by the Association...
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Aflatoxin M1 (AFM1) is founded in milk and other excretion products after aflatoxin B1 intake. AFM1 is carcinogenic to humans, and know levels of dairy products contamination is important to understand the risks that population is exposed. The occurrence of AFM1 was evaluated in 42 milk samples commercialized in Londrina, Paraná State, Brazil and t...
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Sheep milk is the third most consumed milk in Brazil. It is much appreciated for its nutritional status and is important for children that have problems with cow milk. Little information is known about the chemical, physical and microbiological composition of sheep milk from South Brazil. Thus, the aim of this study is to evaluate and characterize...
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O objetivo deste estudo foi avaliar a influencia da contagem de células somáticas (CCS) no leite e o estresse térmico sobre a produção e composição do leite de vacas de um rebanho de produção comercial em região de clima temperado, durante o período de 2008 a 2012. Os dados do dia de controle leiteiro de 161±9 vacas Holandesas, totalizando 9650 ord...
Article
Full-text available
The aim of this research was to identify the occurrence of pathogens causing subclinical mastitis in grade B milk farms of the Jaguapitã county, state of Paraná, Brazil. California Mastitis Test (CMT) were carried out in 400 milk samples from 100 animals and 157 teats from 55 animals (55%) were positive, showed score two or higher to CMT. When thes...
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Antibiotic residues in products of animal origin are of great concern to regulatory agencies and consumers worldwide. Indiscriminate use of antibiotics has promoted the development of bacterial resistance, which has become an international issue. The occurrence of chloramphenicol (CAP) was evaluated in 84 raw milk samples from the reception platfor...
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Background: Mastitis is characterized by inflammation of the mammary gland, usually caused by pathogens, these infections may be classified as either clinical or subclinical, which are responsible for physical, chemical and bacteriological changes in milk and/or changes in the glandular tissue. The shape of both teat and teat tip are among the fact...
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The aim of this study was to verify the effect of inulin and oligofructose on the physicochemical, microbiological and sensory characteristics of symbiotic dairy beverages. Four formulations were made: 1) a control (C); 2) a sample with added Lactobacillus paracasei (P); 3) a sample with added L. paracasei and inulin (PI); and 4) a sample with adde...
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Manteiga é o quinto produto lácteo mais produzido, obtido a partir do creme de leite pasteurizado por processamento tecnológico adequado. O presente trabalho teve como objetivo avaliar a qualidade microbiológica e teores de cloreto de sódio de manteigas com e sem sal aos 30 e 90 dias de armazenamento. Nenhuma amostra apresentou contaminação por Sal...
Article
The aim of this study was to verify the effect of inulin and oligofructose on the physicochemical, microbiological and sensory characteristics of symbiotic dairy beverages. Four formulations were made: 1) a control (C); 2) a sample with added Lactobacillus paracasei (P); 3) a sample with added L. paracasei and inulin (PI); and 4) a sample with adde...
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The objective of this study was to evaluate the quality of lamb meat vacuum packaged (national and imported origin) commercialized in Londrina - PR. Lamb palette samples were evaluated for composition, color parameters, hydrogen potential (pH), loss of water under pressure (LWP) and microbiological quality. The composition of the samples was 70....
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The coagulase test in rabbit plasma tube can be used to distinguish potentially pathogenic staphylococci of harmless. Extraction of rabbit plasma presents difficulties in technique and in the number of animals required. Comparing the detection capacity of the enzyme coagulase produced by Staphylococcus spp., isolated from the cow milk, by rabbit pl...
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O objetivo deste trabalho foi avaliar a qualidade físico-química e microbiológica de amostras de leite UHT integral, comercializados em Londrina, PR, Brasil, assim como a estimativa de geleificação e proteólise durante o período de armazenamento de 150 dias a temperatura ambiente. Análises físico-químicas incluíram determinação da porcentagem de go...
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Traumatic reticular pericarditis is a cardiothoracic lesion that affects cattle and pointed objects are the etiological agents that reach the reticule and the heart triggering cardiac insufficiency and toxemia. It is very important economically because of the loss in production leading to the death of the animal. The clinical signs in these animals...
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Sixteen animals, divided into four groups, underwent pour-on application of cypermethrin so that cypermethrin 5% waiting period in bovine liver could be evaluated. According to recommendations by EMEA/MRL 403/98, maximum limit for cypermethrin for liver tissue is 20μg/Kg. Samples were analyzed by liquid chromatography with mass spectrometry (LC-MS)...
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The aim of this work was to adequate raw refrigerated milk quality of five dairy farms to microbiological and somatic cell count (SCC) standards established by Paraná state legislation. The dairy farms were monitored during seven months after the implementation of good milking practices and recommendations regarding mastitis treatment, and milking...
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Probiotics are live microorganisms capable of producing beneficial effects on its host when consumed in adequate amounts. To exert these effects, foods must contain probiotic microorganisms in populations above 10(6) CFU/g or mL throughout its shelf life. One of the strategies to ensure high population of probiotics in fermented milk is to add them...
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The aim of this work was to verify the viability of Lactobacillus casei in chocolate flan as well as its survival under simulated human gastrointestinal conditions. After 1 and 15 days of manufacture, the dessert was evaluated for L. casei population and subsequently submitted to acidic solution for 30, 60, 90 and 120 min and to bile salts solution...
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This study aimed to compare the microbiological and physicochemical quality of raw milk from individual and collective tanks, identify the psychrotrophic microbiota of refrigerated raw milk and verify compliance with current legislation. The enumeration of proteolytic and lipolytic psychrotrophs, as well as, populations of mesophilic aerobes, total...
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This study evaluated the activity of lactoferrin on the multiplication of probiotic Lactobacillus casei in vitro and in Minas fresh cheese. Growth curves of L. casei in BHI broth containing 1 mg/mL and 2 mg/mL of lactoferrin were performed. Additionally, Minas fresh cheeses added of L. casei (control) and added of L. casei and lactoferrin (2 mg/g a...
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Bovinos holandeses, girolandos e mestiços são os principais responsáveis pela produção de leite no Brasil. A raça e o número de lactações são fatores que podem afetar essa produtividade, bem como características do leite. O objetivo desse trabalho foi avaliar a influência do grupo racial e do número de lactações sobre a produção leiteira, a composi...
Article
Unlabelled: A dried tomato-flavored probiotic cream cheese (P) containing Lactobacillus paracasei Lpc-37 was developed for the purpose of this study. The same product, but without probiotic addition (C) was used as control. Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris were used as lactic starter cultures. Chemical compos...
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The effect of the addition of lactoferrin on Staphylococcus aureus to Minas frescal cheese was evaluated. Three cheeses were made: control (S. aureus), Lf-2% (2% lactoferrin + S. aureus) and Lf-4% (4% lactoferrin + S. aureus). Cheeses were packaged and S. aureus populations were determined on days 1, 8, 15, 22 and 29 of storage at 6 degrees C. The...