Elsa Quillery

Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail | ANSES · Department of Risk Assessment

To deal with the limited literature data on the vectorial capacity of blood‐feeding arthropods (BFAs) and their role in the transmission of African Swine Fever virus (ASFV) in Metropolitan France, a dedicated working group of the French Agency for Food, Environmental and Occupational Health & Safety performed an expert knowledge elicitation. In tot...

African swine fever (ASF) is one of the most important diseases in Suidae due to its significant health and socioeconomic consequences and represents a major threat to the European pig industry, especially in the absence of any available treatment or vaccine. In fact, with its high mortality rate and the subsequent trade restrictions imposed on aff...

African swine fever (ASF) represents a global threat with huge economic consequences for the swine industry. Even though direct contact is likely to be the main transmission route from infected to susceptible hosts, recent epidemiological investigations have raised questions regarding the role of hematophagous arthropods, in particular the stable f...

[This corrects the article DOI: 10.1371/journal.pbio.1002454.].

The arrival of pathogens, whether zoonotic or not, can have a lasting effect on commercial livestock farms, with dramatic health, social and economic consequences. However, available data concerning the arthropod vectors present and circulating on livestock farms in France are still very imprecise, fragmentary, and scattered. In this context, we co...

The tick Ixodes ricinus is the most important vector species of infectious diseases in European France. Understanding its distribution, phenology, and host species use, along with the distribution and prevalence of associated pathogens at a national scale is essential for developing prevention strategies. The aim of this paper is to provide a syste...

The tick Ixodes ricinus is the most important vector species of infectious diseases in European France. Understanding its distribution, phenology, and host species use, along with the distribution and prevalence of associated pathogens at national scales is essential for developing prevention strategies. The aim of this paper is to provide a system...

Current and likely future changes in the geographic distribution of ticks belonging to the genus Hyalomma are of concern, as these ticks are believed to be vectors of many pathogens responsible for human and animal diseases. However, we have observed that for many pathogens there are no vector competence experiments, and that the level of evidence...

Tick-borne encephalitis (TBE) is a viral disease endemic in Eurasia. The virus is mainly transmitted to humans via ticks and occasionally via the consumption of unpasteurized milk products. The European Centre for Disease Prevention and Control reported an increase in TBE incidence over the past years in Europe as well as the emergence of the disea...

At a time of major global, societal, and environmental changes, the shifting distribution of pathogen vectors represents a real danger in certain regions of the world as generating opportunities for emergency. For example, the recent arrival of the Hyalomma marginatum ticks in southern France and the concurrent appearance of cases of Crimean–Congo...

Dans le cadre du plan national de prévention et de lutte contre la maladie de Lyme et les maladies transmissibles par les tiques (« Plan Lyme »)1, lancé en 2016, un projet de recherche bibliographique sur l’écologie, la surveillance et la lutte contre les tiques présentes en France métropolitaine et responsables de maladies infectieuses humaines zo...

Author summary The concurrent circulation of dengue and coronavirus disease 2019 (COVID-19) may produce many unfavourable outcomes—such as co-infections; delays in diagnosis, treatment, and mitigation measures; overwhelming of the healthcare system; underreporting of cases; deterioration in surveillance and control interventions; and exacerbation o...

African swine fever (ASF) represents a global threat with huge economic consequences for the swine industry. Even though direct contact is likely to be the main transmission route from infected to susceptible hosts, recent epidemiological investigations have raised questions regarding the role of hematophagous arthropods, in particular the stable f...

The ability to find and consume nutrient-rich diets for successful reproduction and survival is fundamental to animal life. Among the nutrients important for all animals are polyamines, a class of pungent smelling compounds required in numerous cellular and organismic processes. Polyamine deficiency or excess has detrimental effects on health, cogn...

Olfactory attraction to polyamines. (A) Dose-dependent (0.001–1,000 mM) olfactory preference of Canton S flies to putrescine and cadaverine. Box plots show median and upper/lower quartiles (n = 8, 60 ♀/trial, 30 ♀ and 30 ♂). (B) Analogous to group olfactory behavior, single flies chose polyamine side over non-polyamine side in T-maze assay. Graphs...

Non-Ir41a IR76b-expressing OSNs do not respond to putrescine. (A) Prestimulation fluorescence micrograph showing IR76b OSN axon-innervated glomeruli. VC5 is innervated by IR41a OSNs, which are polyamine responsive. The indicated ROI marks another IR76b innervated glomerulus that was analyzed for a putative response to putrescine. (B) Quantification...

IR76b receptor is required for oviposition preference. (A) Average total number of eggs after 16 h in oviposition assay of Canton S females. Female Canton S flies prefer to lay eggs on the control side (1% low melting agarose only, shown in gray) compared to polyamine side (shown in orange). The number of eggs is averaged for each stimulus (nv = v8...

Egg numbers after 3 h of egg laying. (A–D) Graphs display the number of eggs females laid in the first 3 h of a position–oviposition assay on control (1% low melting agarose) or stimulus (agarose plus polyamines) site corresponding to Fig 5. Note that the low number of eggs in some test lines reflects the slow start of oviposition due to genetic or...

IR76b and Gr66a are not coexpressed in labellar taste neurons. Expression analysis of IR76b (IR76b-QF;QUAS-mdTomato-3xHa) and Gr66a (Gr66a-Gal4;UASmCD8GFP) in proboscis, brain (SEZ), and legs. No coexpression could be observed in neurons of the labellum and axons projecting from these to the SEZ innervated neighboring regions and did not overlap. C...

The excel spreadsheet contains, in separate sheets, the underlying numerical data and statistical analysis for the following figures with their relative panels: Fig 1, Fig 2, Fig 3, Fig 4, Fig 5, Fig 6, S1 Fig, S2 Fig, S3 Fig, S4 Fig, S5 Fig, S6 Fig, S7 Fig and S8 Fig. (XLSX)

IR76b-Gal4 and IR76b-QF show highly overlapping expression pattern. Comparison of GFP/RFP signals in IR76b-QF;QUAS-mdTomato-3xHa and IR76b-Gal4;UASmCD8GFP flies. Neurons in legs, antenna, labellum, and wings always show both GFP and RFP staining. The expression is by and large overlapping with few exceptions where green cells appear to be stained m...

IR76b neurons mediate responses to low salt concentration. (A) Quantification of peak responses of GCaMP6f-fluorescence (in %ΔF/F) in the ROI 1 and ROI 2 areas, respectively, when IR76b-Gal4;UAS-GCaMP6f female flies were stimulated with distilled water or 50 mM NaCl2 (n = 6 ± SEM). (B) Representative image of calcium responses of a leg expressing G...

Structural similarity of NMDA receptor subunit NR1 and polyamine receptors. (A) Schematic presentation of putative structure of the NR1 subunit of the NMDA receptor. Acidic amino acids have been marked in red. In particular, several acidic residues in domains S1, S2, R1, and R2 have been implicated in polyamine-mediated potentiation [69–71]. (B) Pu...

Animals undergo dietary transitions depending on their chang- ing metabolic needs. In humans, pregnant females often experi- ence modulations in their food preference possibly due to their changing needs. Odors and tastes are the main modalities for the evaluation of food. Using the vinegar fly Drosophila mela- nogaster, we have addressed whether...

Detecting single nucleotide polymorphisms (SNPs) between genomes is becoming a routine task with next-generation sequencing. Generally, SNP detection methods use a reference genome. As non-model organisms are increasingly investigated, the need for reference-free methods has been amplified. Most of the existing reference-free methods have fundament...

Ixodes ricinus est la principale espèce vectrice d’agents pathogènes transmises par les tiques en Europe. Une meilleure connaissance de sa variabilité génétique constitue un apport majeur pour le développement de méthodes de lutte (vaccins anti-tiques…). Via les outils de la génétique des populations et la mesure des flux de gènes, elle permet auss...

Assessing the genetic variability of the tick Ixodes ricinus - an important vector of pathogens in Europe - is an essential step for setting up anti-tick control methods. Here we report the first identification of a set of SNPs isolated from the genome of I. ricinus, by applying a reduction of genomic complexity, pyrosequencing and new bioinformati...

In contrast to the prevailing view that invertebrate immunity relies on broad-spectrum recognition and effector mechanisms, intrinsic genetic compatibility between invertebrate hosts and their pathogens is often highly specific in nature. Solving this puzzle requires a better understanding of the molecular basis underlying observed patterns of inve...

Ecology Letters (2012) Epidemiology in host meta-populations depends on parasite ability to disperse between, establish and persist in distinct sub-populations of hosts. We studied the genetic factors determining the short-term establishment, and long-term maintenance, of pathogens introduced by infected hosts (i.e. carriers) into recipient populat...

The environment is rarely constant and organisms are exposed to spatial and temporal variation that will impact life-histories. It is important to understand how such variation affects the adaptation of organisms to their local environment. We compare the adaptation of populations of the ciliate Paramecium caudatum exposed to constant (23 °C or 35...

Parasitic infection can modify host mobility and consequently their dispersal capacity. We experimentally investigated this idea using the ciliate Paramecium caudatum and its bacterial parasite Holospora undulata. We compared the short-distance dispersal of infected and uninfected populations in interconnected microcosms. Infection reduced the prop...