Elizaveta Kulaeva

Princess Máxima Center · Research

Objectives Mutations in ASXL1 are being investigated for prognostic value in AML, but the relationship between these mutations and prognosis for patients with AML remains unclear. Therefore, we are conducting a meta-analysis to estimate the effect of mutations in ASXL1 to determine their prognostic significance. Methods Eight studies were selected...

Children with acute myeloid leukemia (AML) face a relapse of the disease in 30% of all cases. AML relapse is difficult to predict, and existing risk scales are often ineffective. Using data from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET-AML) project, we defined an expression signature based on matrix RNAs (mRN...

Here we investigate the expression of chromatin remodelling protein ATRX in peripheral blood lymphocytes stimulated to fission by PHA.

Cervical cancer is one of the most frequent cancers in women and is associated with human papillomavirus (HPV) in 70 % of cases. Cervical cancer occurs because of progression of low-differentiated cervical intraepithelial neoplasia through grade 2 and 3 lesions. Along with the protein-coding genes, long noncoding RNAs (lncRNAs) play an important ro...

Introduction. DNMT3A is one of the most frequently mutated genes in acute myeloid leukemia (AML), and the R882H (G>A) substitution is one of the most common mutations in this gene. Restriction analysis and high resolution melting (HRM) analysis are the most common methods used to detect this mutation, but the sensitivity of these approaches varies...

The evolution of protein-coding genes has both structural and regulatory components. The first can be assessed by measuring the ratio of non-synonymous to synonymous nucleotide substitutions. The second component can be measured as the normalized proportion of transposable elements that are used as regulatory elements. For the first time, we charac...

Scientific research in recent years has revealed a significant role of the human microbiome in carcinogenesis. These data served as a rationale for the inclusion of polymorphic microbiomes in the key characteristics of carcinogenesis as an important mechanistic determinant of cancer, in addition to other fundamental biological processes manifested...

Цель. Мутации в гене DNMT3A исследуются на предмет прогностической значимости при МДС, но связь между этими мутациями и прогнозом для пациентов остается неясной. Поэтому мы провели метаанализ для оценки влияния мутаций DNMT3A у пациентов с МДС с целью определения их прогностической значимости. Материалы и методы. Были отобраны 12 исследований путем...

Myelodysplastic syndrome is a group of myeloid neoplasms that arise from the action of damaging factors on hematopoietic stem cells, which are based on somatic mutations, which leads to the formation of clonal hematopoiesis. we know from epidemiological data that old age, male gender, and smoking are in themselves independent risk factors for myelo...

Bats are potential natural reservoirs for emerging viruses, causing deadly human diseases, such as COVID-19, MERS, SARS, Nipah, Hendra, and Ebola infections. The fundamental mechanisms by which bats are considered “living bioreactors” for emerging viruses are not fully understood. Some studies suggest that tolerance to viruses is linked to suppress...

Background : Current assessment methods of penile cavernous fibrosis in animal models have limitations due to the inability to provide complex and volume analysis of fibrotic alterations. Objective : The aim was to evaluate micro-computed tomography (micro-CT) for assessment of cavernous fibrosis and compare it with histological, histochemical, im...

Background: Obesity is one of the most common metabolic disorders in the world, which develops due to an imbalance in energy consumption and expenditure, and both genetic and environmental factors are of great importance. We investigated the potential interactions of single nucleotide polymorphisms that might contribute to the development of polyge...

Myelodysplastic syndrome (MDS) is a heterogeneous group of clonal diseases of the blood system arising from a hematopoietic stem cell mutation and characterized by a high risk of transformation into acute leukemia. The disadvantage of the existing test systems for diagnostics of MDDS is the absence of their apparent clinical significance. We focuse...

Development of a test system design for diagnostics and prognosis of MDS based on analysis of common somatic mutations in DNMT3A and ASXL1 genes.

This is the presentation of my 3rd year course project at the Southern Federal University about the expression analysis of the gene ATRX chromatin remodelling protein in human peripheral blood lymphocyes during the cell cycle.

Deoxyribozymes (catalytic DNA) can be used as inactivation method of the fusion protein E6/E7 mRNA of the human papilloma virus. Poster is available in Russian only.