Elizabeth Staley

Elizabeth Staley
University of Washington Seattle | UW · Department of Laboratory Medicine

M.D., Ph.D.

About

51
Publications
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Introduction
Skills and Expertise

Publications

Publications (51)
Article
Patients receiving ABO incompatible (ABOi) kidney transplants are treated pre- and post-transplant with combination therapy, such as intravenous immunoglobulins (IVIG) and therapeutic plasma exchange (TPE), in order to prevent allograft rejection by reducing anti-A and anti-B titers. Although generally considered safe, it is well-known that commerc...
Article
Many studies have suggested that inappropriate plasma usage is common. An important factor contributing to futile plasma administration in most patients is the nonlinear relationship between coagulation-factor levels and the volume of plasma transfused. In this review, a validated mathematical model and data from the literature will be used to illu...
Article
Anti-NMDA (N-methyl D-aspartate) receptor (anti-NMDAR) encephalitis is one of the most common paraneoplastic encephalitides. It occurs in both sexes, across all age ranges, and may occur in the presence or absence of an associated tumor. Its pathogenesis and clinical presentation relate to the presence of IgG1 or IgG3 antibodies targeting the NR1 s...
Article
Full-text available
Immune-mediated thrombotic thrombocytopenic purpura is characterized by severe thrombocytopenia and microangiopathic hemolytic anemia. It is primarily caused by immunoglobin G type autoantibodies against ADAMTS13, a plasma metalloprotease that cleaves von Willebrand factor. However, reliable markers predictive of patient outcomes are yet to be iden...
Article
Objectives: The first coronavirus disease 2019 (COVID-19) case in the United States was reported in Washington State. The pandemic caused drastic disruptions to medical institutions, including medical education. The Department of Laboratory Medicine at the University of Washington responded by rapidly implementing substantial changes to medical st...
Article
Background This report evaluates hospital blood use trends during the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) pandemic, and identifies factors associated with the need for transfusion and risk of death in patients with coronavirus 2019 (COVID‐19). Methods Overall hospital blood use and medical records of adult patients with CO...
Article
Laboratory tests are an integral part of the diagnosis and management of patients; however, these tests are far from perfect. Their imperfections can be due to patient health condition, specimen collection, and/or technological difficulty with performing the assay and/or interpretation. To be useful clinically, testing requires calculation of posit...
Article
Background The first coronavirus (COVID‐19) case was reported in United States (US), in the state of Washington, approximately three months after the outbreak in Wuhan, China. Three weeks later, the US federal government declared the pandemic a national emergency. The number of confirmed COVID‐19 positive cases increased rather rapidly and changed...
Article
Background: Immune thrombotic thrombocytopenic purpura (iTTP) is a potentially fatal syndrome, resulting primarily from autoantibodies against ADAMTS13. However, the mechanism underlying the autoantibody formation and the contribution of other genomic alterations to the pathogenesis of iTTP are largely unknown. Methods: Whole exome sequencing (WES)...
Presentation
Background Immune-mediated thrombotic thrombocytopenic purpura (iTTP) is a potentially fatal syndrome, resulting from autoantibodies against ADAMTS13. However, the mechanism underlying autoantibody formation is not known. Neither is known about the other genetic abnormality in the setting of severe deficiency of plasma ADAMTS13 activity. Methods W...
Article
The safety and integrity of the blood supply is of paramount importance for delivering optimal medical care. Additionally, blood safety is of utmost importance to the United States public. Pathogen testing of donated blood products has been adapted and developed with the goal of eliminating the risk of transfusion-transmitted infectious disease. Cu...
Article
Apheresis is defined as the removal of blood from the body, its separation into constituent components, and removal or manipulation of one of these components prior to intravascular return with or without the addition of replacement fluid. Patients undergoing therapeutic apheresis often have multiple comorbidities, potentially affecting their hemod...
Article
Background: High-quality evidence supporting clinical practice is lacking in apheresis. A potential source of evidence is provided by abstracts submitted to the Annual Meetings of the American Association of Blood Banks (AABB) and the American Society for Apheresis (ASFA). However, there is potential for study conclusions to be altered significant...
Article
Objective: To evaluate how clinical practice was affected by the change in direct antiglobulin testing (DAT) methodologies and subsequent stronger reported DAT results at our large academic medical center. Method: We retrospectively reviewed DAT results of umbilical cord blood from infants with blood type A or B born to mothers with antibody-neg...
Article
The American Society for Apheresis (ASFA)regularly publishes evidence-based guidelines, with the most recent edition in 2016, to assist the requesting and/or apheresis physicians with the evaluation of therapeutic apheresis. Given that therapeutic plasma exchange (TPE)is one of the most common therapeutic apheresis procedures, in this review, we di...
Article
Introduction: The direct antiglobulin test (DAT) detects the presence of IgG and/or C3 on the red blood cell (RBC) membrane and is used to diagnose autoimmune hemolytic anemias as well as hemolytic disease of the fetus and newborn (HDFN). A positive DAT in a neonate occurs when maternal IgG crosses the placenta and binds to fetal RBCs, which may le...
Poster
Background: Immune thrombotic thrombocytopenic purpura (iTTP), a potentially fatal hematological emergency, is primarily caused by acquired deficiency of ADAMTS13 activity due to autoantibodies. Immunoglobulin G (IgG)-type autoantibodies bind ADAMTS13 and inhibit its ability to cleave endothelium-derived ultra large von Willebrand factor (ULVWF). H...
Article
CD5 antigen expression in B‐cell acute lymphoblastic leukemia (B‐ALL) is exceptionally rare. There are six detailed case reports in the literature, with only 16 cases described. Case series analyzing the frequency of aberrant B‐ALL immunophenotypes suggest that this variant may occur in as little as 2‐4.5% of all B‐ALL cases, with one series having...
Article
Many practitioners believe in the phenomenon of being labeled either a “black cloud” or “white cloud” while on‐call. A “white‐cloud” physician is usually defined as one who sees fewer cases while a “black‐cloud” is one who often receives more cases. To evaluate these phenomena, a 35‐month prospective study was designed to evaluate the number of tim...
Article
Heparin-induced thrombocytopenia (HIT) is a not-uncommon adverse effect of heparin exposure, with potentially serious and/or fatal thrombotic consequences. Recent studies looking at the off-label use of fondaparinux for HIT show similar efficacy and adverse-effect profiles, as well as improved costs, compared with some commonly used direct thrombin...
Article
Background B-cell lymphoblastic leukemia (B-ALL) classically presents as a neoplastic proliferation of lymphoblasts expressing B-lineage markers and involving the bone marrow and peripheral blood. Immunophenotypic aberrancies are not uncommon in B-ALL, and coexpression of myeloid lineage markers is not unusual (86.5% of cases). Expression of T-cell...
Article
Full-text available
Background: Chronic myeloid leukemia (CML) is a common hematologic malignancy; however, its occurrence during pregnancy is unusual due to its low prevalence in females of childbearing age. There are conflicting reports of how to best manage CML in pregnancy, particularly in the setting of leukocytosis. Case report: A 30-year-old female was diagn...
Article
Full-text available
Context: - Cytomegalovirus (CMV) can be transmitted by cellular blood products, leading to severe disease in immunosuppressed patients such as neonates and transplant recipients. To mitigate transfusion-transmitted CMV (TT-CMV), "CMV-safe" blood products (leukoreduced and/or CMV-seronegative) are transfused. Attempts to develop practice guidelines...
Article
Full-text available
Background: The ADAMTS13 test distinguishes thrombotic thrombocytopenic purpura (TTP) from other thrombotic microangiopathies (TMAs). The PLASMIC score helps determine the pretest probability of ADAMTS13 deficiency. Due to inherent limitations of both tests, and potential adverse effects and cost of unnecessary treatments, we performed a cost-effe...
Article
Full-text available
The FVB.mdr1a-/- mouse, lacking the small molecule pump P-glycoprotein (P-gp), is a commonly used model for the study of spontaneous T cell mediated colitis. In addition, MDR1 polymorphisms and decreased P-gp function in humans has been linked to the development of ulcerative colitis. Since P-gp is expressed on the apical epithelium of organs and d...
Article
Full-text available
Bone marrow reconstitution is utilized as a tool for disease treatment and as a research technique to elucidate the function of bone marrow derived cells. Clinically successful engraftment is indicated by the development of a functioning immune repertoire. In research, reconstitution is considered successful if >85% of splenic leukocytes are of don...
Article
Full-text available
The FVB.mdr1a(-/-) mouse, lacking the small molecule pump P-glycoprotein (P-gp), is a commonly used model for the study of spontaneous T cell-mediated colitis. In addition, MDR1 polymorphisms and P-gp deficiency in humans have been linked to the development of ulcerative colitis. We now demonstrate that mice with P-gp deficiency have decreased leve...
Article
Full-text available
P-glycoprotein (P-gp) has been reported to increase stem cell proliferation and regulate apoptosis. Absence of P-gp results in decreased repair of intestinal epithelial cells after chemical injury. To further explore the mechanisms involved in the effects of P-gp on intestinal injury and repair, we used the well-characterized radiation injury model...
Article
P-glycoprotein (P-gp), the functional product of the multidrug resistance gene (MDR), is a transmembrane protein that extrudes substrates from the intracellular environment. P-gp is expressed on the apical surface of epithelial cells and on cells from the hematopoietic lineage. Human MDR polymorphisms have been associated with the increased risk of...
Article
Therapy with broad-spectrum antibiotics is a common practice for premature infants. This treatment can reduce the biodiversity of the fecal microbiota and may be a factor in the cause of necrotizing enterocolitis. In contrast, probiotic treatment of premature infants reduces the incidence of necrotizing enterocolitis. We hypothesized that 1 mechani...
Conference Paper
Full-text available
Purpose: Toll-like receptors (TLRs) mediate host-microbial interaction. Premature infants receiving probiotic therapy have a decreased incidence of NEC. The mechanism of this protection may be secondary to intestinal mucosal maturation through commensal bacterial signaling of TLRs 2 and 4. We hypothesized that TLRs 2 and 4 are protective against in...
Article
P-glycoprotein (P-gp), the product of the multidrug resistance gene (MDR), is an ATP-dependent transmembrane pump, which is expressed in multiple cell lineages including epithelial and hematopoetic cells. The human MDR gene is located on chromosome 7 (7q21.1), a susceptibility loci for inflammatory bowel disease (IBD). A significant number of IBD p...
Article
We have developed a simple protocol to transfect mammalian cells using linear polyethylenimine (PEI). Our linear PEI protocol is as effective as commercial reagents in the transfection of HeLa cells and XDC293 cells, a derivative of HEK293 cells, but at a fraction of the cost. Greater than 90% of XDC293 cells and 98% of HeLa cells transfected using...
Article
Recombinant AAV vectors are produced by transient transfection of mammalian cells. The virus is usually purified from a combination of lysed cells and spent culture medium by HPLC. We have developed a quantitative, real-time PCR assay for quantifying encapsidated single-stranded viral DNA (i.e. DNA-containing virions) in cell lysates and the spent...
Article
Background Since the discovery of T helper subsets in 1986, T cells have been classified as TH1 or TH2 on the basis of their cytokine secretion profile. Th1 responses are stimulated by intracellular pathogens and produce high levels of cytokine IFN-g. Dysregulation of the Th1 response has been linked to multiple sclerosis and susceptibility to infl...
Article
Full-text available
We have developed a very simple protocol to transfect mammalian cells with linear polyethylenimine (PEI). Linear PEI has been used to transfect nucleic acids into a wide variety of mammalian cells both in cell culture and in vivo. Almost all of these reports have used commercially available preparations of PEI. Our linear PEI protocol is as efficie...

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Projects

Projects (2)
Project
To understand the pathogenesis of TTP and develop a cure to the disease.