Elizabeth Blaber

USRA/NASA · Space Biosciences Division

Increased oxidative stress is an unavoidable consequence of exposure to the space environment. Our previous studies showed that mice exposed to space for 13.5 days had decreased glutathione levels, suggesting impairments in oxidative defense. Here we performed unbiased, unsupervised and integrated multi-'omic analyses of metabolomic and transcripto...

The International Space Station (ISS) National Laboratory is dedicated to studying the effects of space on life and physical systems, and to developing new science and technologies for space exploration. A key aspect of achieving these goals is to operate the ISS National Lab more like an Earth-based laboratory, conducting complex end-to-end experi...

Hardware testing methods and results. (DOCX)

Detailed description of WetLab-2 system. (DOCX)

Cycle threshold values and p-values for microgravity and 1 g control data. Complete Ct data from E. coli Genomic DNA validation, E. coli, and mouse experiments. Microgravity data are from on-orbit operations while 1 g control are from equivalent experiments run on the ground. Low, Mid, and High represent template concentrations of 0.01, 1.0, and 10...

Background fluorescence data. (PDF)

Mechanical unloading in microgravity during spaceflight is known to cause muscular atrophy, changes in muscle fiber composition, gene expression, and reduction in regenerative muscle growth. Although some limited data exists for long-term effects of microgravity in human muscle, these processes have mostly been studied in rodents for short periods...

Mechanical unloading in microgravity is thought to induce tissue degeneration by various mechanisms, including inhibition of regenerative stem cell differentiation. To address this hypothesis, we investigated the effects of microgravity on early lineage commitment of mouse embryonic stem cells (mESCs) using the embryoid body (EB) model of tissue di...

Abstract Exposure to microgravity causes significant mechanical unloading of mammalian tissues, resulting in rapid alterations of their physiology, which poses a significant risk for long-duration manned spaceflight. The immediate degenerative effects of spaceflight we understand best are those studied during short-term low-Earth-orbit experiments,...

Mechanical loading of mammalian tissues is a potent promoter of tissue growth and regeneration, whilst unloading in microgravity can cause reduced tissue regeneration, possibly through effects on stem cell tissue progenitors. To test the specific hypothesis that mechanical unloading alters differentiation of bone marrow mesenchymal and hematopoieti...

Bone is a dynamically remodeled tissue that requires gravity-mediated mechanical stimulation for maintenance of mineral content and structure. Homeostasis in bone occurs through a balance in the activities and signaling of osteoclasts, osteoblasts, and osteocytes, as well as proliferation and differentiation of their stem cell progenitors. Microgra...

Spaceflight factors, including microgravity and space radiation, have many detrimental short-term effects on human physiology, including muscle and bone degradation, and immune system dysfunction. The long-term progression of these physiological effects is still poorly understood, and a serious concern for long duration spaceflight missions. We hyp...

For thousands of years different cultures around the world have assigned their own meaning to the Universe. Through research and technology, we have begun to understand the nature and mysteries of the Cosmos. Last year marked the 40(th) anniversary of our first steps on the Moon, and within two decades it is hoped that humankind will have establish...

We employed here a global approach to examine the effect of microgravity on a stem cell line, and specific proteins were identified and linked to pathways that are affected by microgravity. This has significant implications to astronaut health.