Eliza B. K. Wolfson

Eliza B. K. Wolfson
University of Bristol | UB · School of Cellular and Molecular Medicine

PhD

About

14
Publications
1,987
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314
Citations
Additional affiliations
October 2008 - April 2014
The Roslin Institute
Position
  • PhD Student

Publications

Publications (14)
Article
Full-text available
Bacterial flagella have many established roles beyond swimming motility. Despite clear evidence of flagella-dependent adherence, the specificity of the ligands and mechanisms of binding are still debated. In this study, the molecular basis of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium flagella binding to epithelial cell cu...
Preprint
Full-text available
Bacterial flagella have many established roles beyond swimming motility. Despite clear evidence of flagella-dependent adherence, the specificity of ligands and mechanisms of binding are still debated. In this study, the molecular basis of E. coli O157:H7 and S. Typhimurium flagella binding to epithelial cell cultures was investigated. Flagella inte...
Article
Full-text available
Vaccines targeting enterohaemorrhagic Escherichia coli (EHEC) O157:H7 shedding in cattle are only partially protective. The correlates of protection of these vaccines are unknown, but it is probable that they reduce bacterial adherence at the mucosal surface via the induction of blocking antibodies. Recent studies have indicated a role for cellular...
Article
Full-text available
The flagellum organelle is an intricate multiprotein assembly best known for its rotational propulsion of bacteria. However, recent studies have expanded our knowledge of other functions in pathogenic contexts, particularly adherence and immune modulation, e.g., for Salmonella enterica, Campylobacter jejuni, Pseudomonas aeruginosa, and Escherichia...
Thesis
Enterohaemorrhagic Escherichia coli (EHEC) have recently emerged as significant zoonotic pathogens. O157:H7 is one of the most common EHEC serotypes associated with human disease, which is transmitted faeco-orally from a bovine reservoir. EHEC O157:H7 preferentially colonises the bovine terminal rectum (BTR). Injection of virulence factors by type-...
Article
Bacterial attachment to plant and animal surfaces is generally thought to constitute the initial step in colonisation, requiring adherence factors such as flagella and fimbriae. We describe the molecular mechanism underpinning flagella-mediated adherence to plant tissue for the food-borne pathogen, enterohemorrhagic Escherichia coli. E. coli H7 fla...
Data
Full-text available
Plasmids used in the study. (PDF)
Data
Full-text available
Oligonucleotide primers used in this study. (PDF)
Data
The role of gadE in Stx phage-based repression of type III secretion. (A) A gadE::gfp reporter (pPgadE.GFP+) and control plasmid (pKC26) (table S2 and [14]) were transformed into E. coli K12 and E. coli K12 (Sp5) and fluorescence measured throughout the growth curve. Data shown is the mean of three cultures with readings at OD420 nm = 0.9. The pres...
Data
Full-text available
Analysis of specific transcript levels in the presence and absence of Stx prophages. (A) ler, espD and gapA (control) transcript levels in relation to 16s rRNA from total RNA extracted from TUV93-0 and a derivative containing the Sp5 Stx2 prophage from Sakai, conjugated into TUV93-0 as described in Materials and Methods. (B) ler, espD and gapA (con...
Data
Full-text available
EHEC O157:H7 PT21/28 and PT32 strains and properties. (PDF)
Data
Full-text available
cII homologs found in O157 strains used in this study. (PDF)
Article
Full-text available
Lytic or lysogenic infections by bacteriophages drive the evolution of enteric bacteria. Enterohemorrhagic Escherichia coli (EHEC) have recently emerged as a significant zoonotic infection of humans with the main serotypes carried by ruminants. Typical EHEC strains are defined by the expression of a type III secretion (T3S) system, the production o...
Article
Type III secretion (T3S) systems enable the injection of bacterial proteins through membrane barriers into host cells, either from outside the host cell or from within a vacuole. This system is required for colonization of their ruminant reservoir hosts by enterohaemorrhagic Escherichia coli (EHEC) and might also be important for the etiology of di...

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