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Elisabete Borges Freitas

Elisabete Borges Freitas
FCT - UNL · BIOENG - UCIBIO (REQUIMTE)

Master in Biotechnology

About

26
Publications
1,977
Reads
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207
Citations
Additional affiliations
June 2019 - May 2022
FCT - UNL
Position
  • Analyst
Description
  • GC, SKALAR and HPLC analysis. Lab management. Fluorescence in situ Hybridization (FISH), fluorescence microscopy and DNA sequencing. Deliverables for European Projects, collaboration in scientific papers and guidance of students.
April 2014 - May 2019
iBET - Instituto de Biologia Experimental e Tecnológica
Position
  • Research Assistant
Description
  • Study of mixed microbial communities through Fluorescence in situ Hybridization, DNA sequencing, clone libraries and qPCR. Responsible for microbiology deliverables for european projects and collaboration in scientific papers.
Education
September 2010 - December 2012
Instituto Superior Técnico - UTL
Field of study
  • Biotechnology
September 2006 - July 2010
Faculty of Sciences - University of Lisbon
Field of study
  • Evolutionary and Developmental Biology

Publications

Publications (26)
Article
Diclofenac is a pharmaceutical active compound frequently detected in wastewater and water bodies, and often reported to be persistent and difficult to biodegrade. While many previous studies have focussed on assessing diclofenac biodegradation in nitrification and denitrification processes, this study focusses on diclofenac biodegradation in the e...
Article
In enhanced biological phosphorus removal (EBPR), Tetrasphaera can potentially be an abundant and important polyphosphate accumulating organism (PAO), however ongoing questions remain concerning its storage compounds, phosphorus (P) removal capabilities and metabolic behaviour. This study investigated each of these points in an enriched Tetrasphaer...
Article
The process involving mixed microbial cultures (MMCs) and waste-based substrates emerged as an alternative solution to reduce the market price of polyhydroxyalkanoates (PHAs). The selection of an efficient MMC that displays a significant PHA accumulation potential and a high growth rate is considered a key factor for the MMC PHA production feasibil...
Article
Accumulibacter is a well-known group of organisms, typically considered to be polyphosphate accumulating organisms (PAOs), but potentially capable of glycogen accumulating organism (GAO) metabolism under limiting influent phosphate levels. Metabolic features of Accumulibacter are typically linked to its phylogenetic identity at the Type or clade le...
Article
Anaerobic digestion has been shown to be an effective treatment for high organic matter wastes, while producing clean energy. However, anaerobic digesters in some companies must support variations of substrate composition due to the seasonality of feedstocks. In this study, a two-stage anaerobic digestion system was operated treating three differen...
Article
This work is focused on production of volatile fatty acids (VFA) through anaerobic digestion (AD) using raw (without pre-treatment) brewers' spent grain (BSG) as feedstock. VFAs are by-products from AD of organic wastes with wide potential industrial application in bioplastic production. A long term fed batch stirred-tank reactor was operated and t...
Presentation
The results presented show that it is possible to select a Photo-EBPR system directly from a WWTP sludge. However, the selection strategy used requires more time than use a sludge already enriched in polyphosphate accumulating organisms.
Presentation
Phosphorus is an element with vast applications in agriculture and industry but that can negatively impact the environment when released to water streams. Reserves of P are limited, making it crucial to treat P rich wastewater streams, preferably through processes that allow P recovery and reutilization, to promote the circular economy and an envir...
Article
An indigenous plant growth promoting bacterium isolated from Peganum Harmala rhizosphere in arid ecosystem was found to solubilise and accumulate phosphates. This isolate was identified as Pseudomonas sp. (PHR6) by partial 16S rRNA gene sequence analysis. Controlled batch experiments on nutrients removal by this isolate in mineral medium showed rel...
Poster
An innovative Phototrophic - Enhanced Biological Phosphorus Removal (Photo-EBPR) system, consisting of a consortium of photosynthetic organisms and polyphosphate accumulating organisms (PAOs), was studied in this work. A sequencing batch reactor fed with a mixture of acetate and propionate was subjected to dark/light cycles in order to select a pho...
Article
The phototrophic-enhanced biological phosphorus removal system (photo-EBPR) was recently proposed as an alternative photosynthetic process to conventional phosphorus removal. Previous work showed the possibility of obtaining a photo-EBPR system starting from a culture already enriched in polyphosphate accumulating organisms (PAOs). The present work...
Presentation
Reduce the aeration necessities during EBPR treatment using a consortium of PAOs and photosynthetic microorganisms that provide the necessary oxygen for P uptake with no need of external aeration
Article
Food and beverage industry wastes present high amounts of organic matter, which may cause water quality degradation if not treated. Two-stage anaerobic digestion is a promising and efficient solution for the treatment of this type of wastes whilst producing bioenergy. The composition of fruit pulp waste varies throughout the different harvesting se...
Article
A novel Phototrophic - Enhanced Biological Phosphorus Removal (Photo-EBPR) system, consisting of a consortium of photosynthetic organisms and polyphosphate accumulating organisms (PAOs), was studied in this work. A sequencing batch reactor was fed with a mixture of acetate and propionate (75%-25%) and subjected to dark/light cycles in order to sele...
Presentation
Phosphorus (P) is an element with an increasing utilization worldwide and a high usage in chemical, food and, particularly, in agronomical industries. For this reason, P can be frequently found in water streams in concentrations susceptible of causing eutrophication and environmental problems. It is essential to treat the P containing streams and,...
Article
Polyhydroxyalkanoates (PHAs) are biobased and biodegradable polyesters with the potential to replace conventional plastics. Aeration requires large amounts of energy in PHA production by mixed microbial cultures (MMCs), particularly during the feast phase due to substrate uptake. The objective of this study was to investigate the impact of DO conce...
Article
Polyhydroxyalkanoates (PHA) are a sustainable alternative to conventional plastics that can be obtained from industrial wastes/by-products using mixed microbial cultures (MMC). MMC PHA production is commonly carried out in a 3-stage process of acidogenesis, PHA culture selection and accumulation. This research focused on the possibility of tailorin...
Article
Immune response against an encoded antigenic protein can be elicited by including targeting sequences to DNA vaccines that promote protein sorting to processing pathways, related with antigen presentation by major histocompatibility complexes (MHC). Candidate DNA vaccines coding for neuraminidase 3 of the avian influenza virus were designed to enco...

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Projects

Project (1)
Project
PHA production MMCs wastewater modelling