Elijah Miinda Ateka

Elijah Miinda Ateka
Jomo Kenyatta University of Agriculture and Technology | JKUAT · Department of Horticulture

PhD

About

230
Publications
33,697
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,250
Citations

Publications

Publications (230)
Article
The production of sweet potato has the potential to address food insecurity and malnutrition in the context of climate change. However, sustainable production of sweet potato is hampered by poor access to quality seed. We assess the level and deter-minants of farmers’ willingness to pay (WTP) for clean seed among smallholder sweet potato farmers an...
Article
Full-text available
Background Tomato production is threatened worldwide by the occurrence of begomoviruses which are associated with tomato leaf curl diseases. There is little information on the molecular properties of tomato begomoviruses in Kenya, hence we investigated the population and genetic diversity of begomoviruses associated with tomato leaf curl in Kenya....
Article
Full-text available
In the context of climate change and its effects, production and consumption of orphaned crops such as sweet potato has been promoted as alternative food diets in sub-Saharan Africa (SSA). However, sustainable production of these crops is hampered by poor access to seed. This paper assesses seed security among smallholder sweet potato producers in...
Article
Full-text available
Cassava brown streak disease (CBSD) is an economically important disease of cassava (Manihot esculenta crantz) caused by Cassava brown streak virus (CBSV). Use of clean planting material is one of the strategies for disease management. However, obtaining clean planting material for some farmer-preferred varieties is often difficult. This research w...
Article
Full-text available
Background Cassava is a staple food for over 800 million people globally providing a cheap source of carbohydrate. However, the cultivation of cassava in the country is facing to viral diseases, particularly cassava mosaic disease (CMD) which can cause up to 95% yield losses. With aim to supply farmers demand for clean planting materials, there is...
Article
Full-text available
In 2017 in Kenya, we collected leaves from tomato plants with leaf curling. The leaves were infected with a begomovirus that was most similar to tomato leaf curl Uganda virus (ToLCUV-[UG:Iga:05]). Neither DNA-B nor satellite DNAs were detected. Recombination analyses indicated the virus was a chimera of tomato leaf curl Madagascar virus (ToLCMGV-At...
Preprint
Full-text available
Background Tomato production is threatened worldwide by the occurrence of begomoviruses which are associated with tomato leaf curl diseases. There is little information on the molecular properties of tomato begomoviruses in Kenya, hence we investigated the population and genetic diversity of begomoviruses associated with tomato leaf curl in Kenya....
Preprint
Full-text available
Background: Tomato production is threatened worldwide by the occurrence of begomoviruses which are associated with tomato leaf curl diseases. There is little information on the molecular properties of tomato begomoviruses in Kenya, hence we investigated the population and genetic diversity of begomoviruses associated with tomato leaf curl in Kenya....
Article
This study aimed to assess elite cassava genotypes for resistance to cassava mosaic and brown streak diseases at Alupe, Kakamega and Kibos in Western Kenya. The trial was conducted using alpha lattice balanced design using 24 genotypes with three replicates, for an extended cropping cycle between 2016 and 2017. Results for combined analysis of vari...
Preprint
Full-text available
Background Cassava is a staple food for over 800 million people globally providing a cheap source of carbohydrate. However, the cultivation of cassava in the country is facing to viral diseases, particularly cassava mosaic disease (CMD) which can cause up to 95% yield losses. With aim to supply farmers demand for clean planting materials, there is...
Preprint
Full-text available
Background Cassava is a staple food for over 800 million people globally providing a cheap source of carbohydrate. However, the cultivation of cassava in the country is facing to viral diseases, particularly cassava mosaic disease (CMD) which can cause up to 95% yield losses. With aim to supply farmers demand for clean planting materials, there is...
Preprint
Full-text available
Background Cassava is a staple food for over 800 million people globally providing a cheap source of carbohydrate. However, the cultivation of cassava in the country is facing to viral diseases, particularly cassava mosaic disease (CMD) which can cause up to 95% yield losses. With aim to supply farmers demand for clean planting materials, there is...
Preprint
Full-text available
Background Cassava is a staple food for over 800 million people globally providing a cheap source of carbohydrate. However, the cultivation of cassava in the country is facing to viral diseases, particularly cassava mosaic disease (CMD) which can cause up to 95% yield losses. With aim to supply farmers demand for clean planting materials, there is...
Article
Cassava (Manihot esculenta Crantz) production in sub-Saharan Africa (SSA) is constrained by the two biotic constraints namely, cassava mosaic disease (CMD) and cassava brown streak disease (CBSD). The aim of this study was to evaluate elite cassava genotypes for variation in agronomical traits, correlate them to CMD and CBSD parameters and identify...
Article
Full-text available
Carica papaya L. is an important fruit crop grown by small-and large-scale farmers in Kenya for local and export markets. However, its production is constrained by papaya ringspot disease (PRSD). The disease is believed to be caused by papaya ringspot virus (PRSV). Previous attempts to detect PRSV in papaya plants showing PRSD symptoms, using enzym...
Article
Full-text available
Cassava is a staple food crop in sub-Saharan Africa; it is a rich source of carbohydrates and proteins which currently supports livelihoods of more than 800 million people worldwide. However, its continued production is at stake due to vector-transmitted diseases such as Cassava mosaic disease and Cassava brown streak disease. Currently, the manage...
Article
Full-text available
High-throughput sequence analysis revealed the complete genome sequence of a novel, hitherto uncharacterized strain of Chickpea chlorotic dwarf virus (CpCDV) from tomato plants in Kenya. The sequence shared its highest nucleotide similarity (88.7%) with two CpCDV isolates from Burkina Faso.
Article
Full-text available
This study investigated Tomato chlorosis virus (ToCV) present in plants collected in September 2017, in Kenya. We collected tomato leaves that exhibited severe interveinal yellowing symptoms and used reverse transcription polymerase chain reaction (RT-PCR) to confirm that 15 of the 36 samples were infected with ToCV. One sample collected in Kiambu...
Article
Full-text available
In this study, the effect of a Kenyan strain of Sweetpotato leaf curl virus (SPLCV) and its interactions with Sweetpotato feathery mottle virus (SPFMV) and Sweetpotato chlorotic stunt virus (SPCSV) on root yield was determined. Trials were performed during two seasons using varieties Kakamega and Ejumula and contrasting in their resistance to sweet...
Preprint
Full-text available
The effect of a Kenyan strain of sweetpotato leaf curl virus (SPLCV) and its interactions with sweetpotato feathery mottle virus (SPFMV), and sweetpotato chlorotic stunt virus (SPCSV) on root yield was determined. Trials were performed during two seasons using varieties Kakamega and Ejumula contrasting in their resistance to sweetpotato virus disea...
Article
Full-text available
Cassava brown streak disease is caused by cassava brown streak virus (CBSV) and Uganda cassava brown streak virus (UCBSV). Many of the CBSV and UCBSV diversity studies utilize partial coat protein sequences due to the unavailability of representative full genome sequences. Hence, there is little information on the diversity of cassava brown streak...
Article
Full-text available
In this case study we successfully teamed the PDQeX DNA purification technology developed by MicroGEM, New Zealand, with the MinION and MinIT mobile sequencing devices developed by Oxford Nanopore Technologies to produce an effective point-of-need field diagnostic system. The PDQeX extracts DNA using a cocktail of thermophilic proteinases and cell...
Preprint
Full-text available
In this case study we successfully teamed the PDQeX DNA purification technology developed by MicroGEM, New Zealand, with the MinION and MinIT mobile sequencing devices developed by Oxford Nanopore Technologies to produce an effective point-of-need field diagnostic system. The PDQeX extracts DNA using a cocktail of thermophilic proteinases and cell...
Article
Full-text available
Common bean (Phaseolus vulgaris L.) is the primary source of protein and nutrients in the majority of households in sub-Saharan Africa. However, pests and viral diseases are key drivers in the reduction of bean production. To date, the majority of viruses reported in beans have been RNA viruses. In this study, we carried out a viral metagenomic ana...
Article
Full-text available
Plant viral diseases are one of the major limitations in legume production within sub-Saharan Africa (SSA), as they account for up to 100% in production losses within smallholder farms. In this study, field surveys were conducted in the western highlands of Kenya with viral symptomatic leaf samples collected. Subsequently, next-generation sequencin...
Article
Full-text available
Various modes of edible coating application vary in their coat dispersion and film formation, hence the need to determine the most effective mode of application for cassava. Edible surface coatings have been found to be effective in preserving the quality of various food products. However, there are variations in effectiveness among the different c...
Article
Full-text available
Cassava brown streak disease (CBSD) caused by Cassava brown streak virus (CBSV) and Uganda cassava brown streak virus (UCBSV) is a major constraint to cassava production in Mozambique. Full genome sequences of CBSD‐associated virus isolates contribute to the understanding of genetic diversity and the development of new diagnostic primers that can b...
Article
Full-text available
Cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) are two viral diseases that cause severe yield losses in cassava of up to 100%, thereby persistently threatening food and income security in sub-Saharan Africa. For effective management of these diseases, there is a critical need to develop and deploy varieties with dual resistanc...
Article
Full-text available
Cassava brown streak disease (CBSD) caused by Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) is the main constraint to cassava (Manihot esculenta Crantz) production in Mozambique. Using RT-PCR to amplify partial coat protein nucleotide sequences, we detected for the first time the occurrence of CBSV in two non-cass...
Article
Full-text available
Background: Cassava brown streak disease (CBSD) has a viral aetiology and is caused by viruses belonging to the genus Ipomovirus (family Potyviridae), Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Molecular and serological methods are available for detection, discrimination and quantification of cassava brown st...
Article
Full-text available
Viral symptomatic groundnut (Arachis hypogaea L.) leaf samples were collected in the heterogeneous agro-ecosystem of the western highlands of Kenya. High throughput RNA sequencing (RNA-Seq) was carried out on total plant RNA using the Illumina HiSeq platform. Subsequently, de novo assembly and sequence similarity searches identified the complete ge...
Article
Full-text available
Cassava mosaic disease is caused by cassava mosaic begomoviruses (CMBs) and can result in crop losses up to 100% in cassava (Manihot esculenta) in Tanzania. We investigated the efficacy of chemotherapy and thermotherapy for elimination of East African cassava mosaic virus (EACMV) of Tanzanian cassava. In vitro plantlets from EACMV‐infected plants o...
Article
Full-text available
Sweet potato is a major food security crop within sub-Saharan Africa where 90% of Africa production occurs. One of the major limitations of sweet potato production are viral infections. In this study, we used a combination of whole genome sequences from a field isolate obtained from Kenya and those available in GenBank. Sequences of four sweet pota...
Chapter
Arising from a relatively isolated center of origin, papaya has spread throughout all tropical and subtropical countries through human intervention. This global dispersal has coincided with continuous improvement of the cultivated plants through breeding programs often designed to improve the agronomic characters and to address biotic and abiotic s...
Data
Table S2a. Representation of clades resolved across the gene trees and the respective posterior probability in the Sweet potato virus C (SPVC) and Sweet potato feathery mottle virus (SPFMV). Only clades with posterior probability of above 0.7 were considered.
Data
Table S2b. Clades resolved across the gene trees and respective posterior probability across the Sweet potato chlorotic fleck virus (SPCFV) and Sweet potato chlorotic stunt virus (SPCSV). Only clades with posterior probability of above 0.7 were considered.
Data
Table S1. Summary of whole genome sequences from this study and from GenBank used for phylogenetic analysis and selection pressure analysis.
Data
Fig. S5. Species tree generated from SVD Quartets using the complete genome of Sweet potato feathery mottle virus (SPFMV) with Sweet potato virus C (SPVC) used as an outgroup to root the tree. The nodes across each branch are labeled with bootstrap values. Tip labels contain information of: virus name, GenBank accession number and/or field identifi...
Data
Fig. S4. Species tree generated from SVD Quartets using the complete genome of Sweet potato virus C (SPVC) sequences with the Sweet potato feathery mottle virus (SPFMV) (FJ155666) used as the outgroup to root the tree.
Data
Fig. S1. Species tree generated from SVD Quartets using the complete genome RNA1 of Sweet potato chlorotic stunt virus (SPCSV) with the Cucurbit chlorotic yellows virus (CCYV) (NC018174) as the outgroup to root the tree. The nodes across each branch are labeled bootstrap values. Tip labels contain information of: virus name, GenBank accession numbe...
Data
Fig. S2. Species tree generated from SVD Quartets using the complete genome RNA2 of Sweet potato chlorotic stunt virus (SPCSV) with the Cucurbit chlorotic yellows virus (CCYV) (JQ904628) as the outgroup to root the tree. The nodes across each branch are labelled with bootstrap values. Tip labels contain information of: virus name, GenBank accession...
Data
Fig. S3. Species tree generated from SVD Quartets using the complete genome of Sweet potato chlorotic fleck virus (SPCFV) with the Pea Streak virus (NC027527) used as the outgroup to root the tree. The nodes across each branch are labeled with bootstrap values. Tip labels contain information of: virus name, GenBank accession number and/or field ide...
Article
Full-text available
Crop losses due to viral diseases and pests are major constraints on food security and income for millions of households in sub-Saharan Africa (SSA). Such losses can be reduced if plant diseases and pests are correctly diagnosed and identified early. Currently, accurate diagnosis for definitive identification of plant viruses and their vectors in S...
Article
Full-text available
Sweetpotato (Ipomoea batatas) is a vital crop for overcoming food insecurity in sub-Saharan Africa and its production is highest in East Africa where yields are high and the growing seasons are short. This cross-country study assessed farmers’ local practices and their knowledge of the biotic constraints to sweetpotato production in Uganda, Rwanda,...
Preprint
Full-text available
The United Nations has listed Zero Hunger as one of the 17 global sustainable development goals to end extreme poverty by 2030. Plant viruses are a major constraint to crop production globally causing an estimated $30 billion in damage leaving millions of people food insecure. In Africa, agriculture employs up to 50% of the workforce, yet only cont...
Preprint
Full-text available
Sweet potato is a major food security crop within sub-Saharan Africa where 90 % of Africa’s sweet potato production occurs. One of the major limitations of sweet potato production are viral infections. In this study, we used a combination of whole genome sequences from a field isolate from Kenya and those available in GenBank. Sequences of four swe...
Preprint
Full-text available
Sweet potato is a major food security crop within sub-Saharan Africa where 90 % of Africa’s sweet potato production occurs. One of the major limitations of sweet potato production are viral infections. In this study, we used a combination of whole genome sequences from a field isolate from Kenya and those available in GenBank. Sequences of four swe...
Preprint
Full-text available
Background:Endornavirus es are non-pathogenic viruses infecting multiple agricultural important crops including legumes, with global distribution. However, there is an absence on the complete genome of endornaviruses from legumes in particular with the sub-Saharan region. In this study, we report the first complete genomes of PvEV1 and PvEV2, and t...
Preprint
Background:Endornavirus es are non-pathogenic viruses infecting multiple agricultural important crops including legumes, with global distribution. However, there is an absence on the complete genome of endornaviruses from legumes in particular with the sub-Saharan region. In this study, we report the first complete genomes of PvEV1 and PvEV2, and t...