Eliane Tabea Taube

• Pathologist
• Medical Doctor at Diagnostik Ernst von Bergmann

Head and neck squamous cell carcinoma (HNSCC) with discordant diagnostic patterns of HPV+/p16− or HPV−/p16+ correlate with worse prognosis. This study aims to identify truly HPV-driven HNSCCs using a QuantiGene-Molecular-Profiling-Histology (QG-MPH) assay for identifying transcriptionally active HPV. Of 97 FFPE samples analyzed, 68 were valid for H...

Background We have recently constructed a DNA methylation classifier that can discriminate between pancreatic ductal adenocarcinoma (PAAD) liver metastasis and intrahepatic cholangiocarcinoma (iCCA) with high accuracy (PAAD-iCCA-Classifier). PAAD is one of the leading causes of cancer of unknown primary and diagnosis is based on exclusion of other...

Background Head and neck squamous cell carcinoma (HNSCC) with discordant diagnostic pat-terns of HPV⁺/p16⁻ or HPV⁻/p16⁺ correlate with worse prognosis. This study aims to identify truly HPV-driven HNSCCs using mRNA profiling by QuantiGene Molecu-lar-Profiling-Histology (QG-MPH) assay for more precise etiological diagnosis. Methods Of 97 FFPE sample...

Disease progression is a major problem in ovarian cancer. There are very few treatment options for patients with platinum‐resistant ovarian cancer (PROC), and therefore, these patients have a particularly poor prognosis. The aim of the present study was to identify markers for monitoring the response of 123 PROC patients enrolled in the Phase I/II...

Background The aim of this study was to analyse transcriptomic differences between primary and recurrent high-grade serous ovarian carcinoma (HGSOC) to identify prognostic biomarkers. Methods We analysed 19 paired primary and recurrent HGSOC samples using targeted RNA sequencing. We selected the best candidates using in silico survival and pathway...

Background The enzyme indoleamine 2,3-dioxygenase 1 (IDO1) plays a crucial role in regulating the immune system's response to tumors, but its exact role in cancer, especially in high-grade serous ovarian cancer (HGSOC), remains controversial. We aimed to investigate the prognostic impact of IDO1 expression and its correlation with tumor-infiltratin...

Background Mechanisms of development and progression of high-grade serous ovarian cancer (HGSOC) are poorly understood. EVI1 and PARP1, part of TGF-ß pathway, are upregulated in cancers with DNA repair deficiencies with DNA repair deficiencies and may influce disease progression and survival. Therefore we questioned the prognostic significance of p...

RGS2 regulates G-protein signaling by accelerating hydrolysis of GTP and has been identified as a potentially druggable target in carcinomas. Since the prognosis of patients with high-grade serous ovarian carcinoma (HGSOC) remains utterly poor, new therapeutic options are urgently needed. Previous in vitro studies have linked RGS2 suppression to ch...

Background This study seeks to evaluate the impact of breast cancer ( BRCA ) gene status on tumor dissemination pattern, surgical outcome and survival in a multicenter cohort of paired primary ovarian cancer (pOC) and recurrent ovarian cancer (rOC). Patients and Methods Medical records and follow-up data from 190 patients were gathered retrospecti...

Purpose In recent years the tumor microenvironment and its interaction with the tumor has emerged into research focus with increased attention to the composition of Tumor-infiltrating lymphocytes. We wanted to quantify the composition of Regulatory T cells (Tregs) and T helper 17 cells (Th17 cells) and their prognostic impact in high-grade serous t...

Background High-grade serous ovarian cancer (HGSOC) is the most common subtype of ovarian cancer and is associated with high mortality rates. Surgical outcome is one of the most important prognostic factors. There are no valid biomarkers to identify which patients may benefit from a primary debulking approach. Objective Our study aimed to discover...

Background/aim: Mucinous ovarian carcinoma (mOC) is a rare subtype with distinct clinical characteristics and biological behavior that differentiate them from other epithelial ovarian cancers. This study aimed to evaluate BMI-1 expression as a potential target for therapeutic approaches in advanced stage mOC. Materials and methods: We performed...

The particularly high mortality of epithelial ovarian cancer (EOC) is in part linked to limited understanding of its molecular signatures. Although there are data available on in situ N-glycosylation in EOC tissue, previous studies focused primarily on neutral N-glycan species and, hence, still little is known regarding EOC tissue-specific sialylat...

Endometriosis (EM) is an estrogen-dependent disease characterized by the presence of epithelial, stromal, and smooth muscle cells outside the uterine cavity. It is a chronic and debilitating condition affecting ~10% of women. EM is characterized by infertility and pain, such as dysmenorrhea, chronic pelvic pain, dyspareunia, dysuria, and dyschezia....

Background Current clinical guidelines suggest that breast cancers with low hormone receptor expression (LowHR) in 1–10% of tumor cells should be regarded as hormone receptor positive. However, clinical data show that these patients have worse outcome compared to patients with hormone receptor expression above 10%. We performed DNA methylation prof...

Introduction/Background* Mesothelin (MSLN) is a CA125 binding protein that mediates cell adhesion. This interaction was suggested to play a role in the peritoneal metastasis development. In preclinical models, MSLN overexpression activates the PI3K/AkT, NFκB, and MAPK/ERK pathways, to promote cell proliferation, migration and metastasis. For these...

We conducted a meta-analysis of published data to update and estimate the prevalence of HPV in ovarian cancer. A comprehensive literature search was performed according to the PRISMA guidelines. Eligible articles published from 1989 until 2020 by searching Web of Sciences, Pubmed, Embase, and the Cochrane Library Central databases were gathered. A...

[This corrects the article DOI: 10.1371/journal.pone.0238021.].

Purpose To analyze the follow-up results of patients suffering from symptomatic early-stage endometriosis after a consistent laparoscopic peritoneal stripping of the altered peritoneum (peritoneal endometriosis and surrounding inflamed tissue) was performed. This type of endometriosis is resistant to medical therapy and/or impairs fertility. Metho...

e17544 Background: High-grade serous ovarian cancer (HGSOC) can be separated by gene expression profiling into four molecular subtypes with clear correlation of the clinical outcome. However, these gene signatures have not been implemented in clinical practice to stratify patients for targeted therapy. This is mainly due to a lack of easy, cost-eff...

Despite the correlation of clinical outcome and molecular subtypes of high-grade serous ovarian cancer (HGSOC), contemporary gene expression signatures have not been implemented in clinical practice to stratify patients for targeted therapy. Hence, we aimed to examine the potential of unsupervised matrix-assisted laser desorption/ionization imaging...

The image illustrates the potential of MALDI Imaging to explore differences in spatial proteomic alterations in aneurysmal ascending aortic tissues from patients with aortic valve vitium (BAV), normal aortic valve (TAV), and aneurysmal tissue of connective tissue disorder (MFS). MALDI Imaging in combination with histopathological assessment are fea...

The differential diagnosis of thyroid nodules in patients treated for renal cell carcinoma in the past can be challenging unless they present sonographic features of malignancy. The possibility of metastases to the thyroid gland, even many years after the primary occurrence of the renal tumor, should always be considered. Here, we present the case...

Purpose Histopathological evaluation presents conflicting reports regarding aortic abnormalities. The authors aim to present proof‐of‐concept study to explore the feasibility of matrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI‐IMS) in combination with histopathology for characterizing alterations in the aneurysmal ascend...

Introduction/Background PARP inhibitors (PARPi) have been established as a targeted therapeutic approach not only in patients with high-grade serous ovarian cancer (HGSOC) that have genetic loss of function of BRCA1/2-associated DNA repair. However, treatment efficacy varies and neither BRCA mutation, nor homolog recombination deficiency (HRD) stat...

Objective Low-grade serous ovarian cancers (LGSOC) characterize less frequent incidence of lymph-nodes (LN) metastasis. Ki67 expression level is associated with prognosis and therapy outcome differences. Its’ expression in combination with lympho-vascular space invasion (LVSI) have not been evaluated in prediction of LN involvement yet. Methods Pa...

Objective Low grade serous ovarian cancers characterize a unique clinical pattern and likely less frequent incidence of lymphatic metastasis. The expression level of Ki67 is associated with differences in prognosis and therapy outcome. However, its expression in combination with lymphovascular space invasion has not been evaluated in the prediction...

Background/aim: Angiosarcoma of primary gynecologic origin is an extremely rare and highly malignant tumor of endothelial origin with a 5-year survival rate of less than 35%. To date, only 61 cases have been described in the literature. The aim of this study was to present more cases and discuss potential therapy options. Case report: The follow...

Triple-negative breast cancer (TNBC) is typically treated with chemotherapeutic agents, including carboplatin (Cb), an DNA platinating agent. The O6-methylguanine-DNA-methyltransferase gene (MGMT) encodes for the protein O6-alkylguanine-DNA-alkyltransferase (MGMT protein). MGMT protein is involved in DNA repair mechanisms to remove mutagenic and cy...

With regard to relapse and survival, early-stage high-grade serous ovarian (HGSOC) patients comprise a heterogeneous group and there is no clear consensus on first-line treatment. Currently, no prognostic markers are available for risk assessment by standard targeted immunohistochemistry and novel approaches are urgently required. Here, we applied...

Erratum zu: Im Fokus Onkologie. 2017;20(12):32-7In (S. 35) des Beitrages in der Rubrik Zertifizierte Fortbildung hatte sich ein Fehler eingeschlichen. Maligner Aszites macht etwa 10 % aller Aszitesfälle aus.Wir bitten diesen Fehler zu entschuldigen. Die Redaktion

6078 Background: PARP inhibitors (PARPi) have been established as a targeted therapeutic approach not only in patients with high-grade serous ovarian cancer (HGSOC) that have genetic loss of function of BRCA1/2-associated DNA repair. However, treatment efficacy varies and neither BRCA mutation, nor homolog recombination deficiency (HRD) status seem...

High-grade serous ovarian cancer (HGSOC) likely originates from the fallopian tube (FT) epithelium. Here, we established 15 organoid lines from HGSOC primary tumor deposits that closely match the mutational profile and phenotype of the parental tumor. We found that Wnt pathway activation leads to growth arrest of these cancer organoids. Moreover, a...

Objective Low-grade serous ovarian cancers characterize a unique clinical pattern and lower chemotherapy responsiveness. The expression level of Ki67 is associated with differences in prognosis; however, this has not yet been evaluated in regard to predicting the outcome of therapy. Methods Patients with low-grade serous ovarian cancers were ident...

Background: Clinical application of cancer immunotherapy requires a better understanding of tumor immunogenicity and the tumor microenvironment. HLA class I molecules present antigens to CD8+ cytotoxic cells. Their loss or downregulation is frequently found in tumors resulting in reduced T cell responses and worse prognosis. Methods: We evaluate...

Background/aim: Involvement of lymph nodes (LNs) and their surgical resection in low-grade ovarian cancer remains a field of discussion. The aim of this study was to determine the incidence and pattern of distribution of lymph node metastases in patients with low-grade serous ovarian cancer. Patients and methods: A retrospective analysis was car...

High-grade serous ovarian cancer (HGSOC) likely originates from the fallopian tube (FT) epithelium. Here, we established 15 organoid lines from HGSOC primary tumor deposits that closely match the parental tumor mutational profile and phenotype. We found that Wnt pathway activation leads to growth arrest of these cancer organoids. Moreover, active B...

Background: Checkpoint-Inhibition has revolutionized the treatment for several entities such as melanoma and renal cell carcinoma. The first encouraging experience in ovarian cancer was reported for nivolumab, a fully humanized anti-programmed death-1 antibody. Pseudoprogression is a new phenomenon associated with these novel immuno-oncologic agen...

e17091 Background: Previously four molecular subtypes of high grade serous ovarian cancer (HGSOC) with distinct biological features and clinical outcome have been described: C1 (mesenchymal), C2 (immunoreactive), C4 (differentiated), and C5 (proliferative). Using Nanostring technique and a minimal signature of 39 classifier genes could reproduce th...

5577 Background: Low-grade serous ovarian cancers (LGSOC) characterize different clinical pattern and lower chemotherapy responsiveness. The expression level of Ki67 is associated with prognosis differences in this patient group. However, Ki67 has not been evaluated as prognostic marker and a predictor of therapy outcome until now. Methods: Patient...

Background Aortic stenosis is a common finding in cardiac amyloidosis (CA). Younger patients often remain asymptomatic. If unrecognized, this can lead to serious complications such as heart failure. Progression of aortic stenosis can be accelerated in patients with chronic kidney disease and need for dialysis. Perioperative risk in these patients i...

Objective The impact of angiogenesis on long-term survival of high-grade serous ovarian cancer (HGSOC) patients remains unclear. This study investigated whether angiogenic markers correlated with 5-year progression-free survival (PFS) in a large cohort of matched advanced HGSOC tissue samples. Methods Tumor samples from 124 primary HGSOC patients...

Precise histological classification of epithelial ovarian cancer (EOC) subtypes is important for personalized treatment. The image, designed by Maria Hänel, illustrates the potential of imaging mass spectrometry combined with machine learning methods to explore spatial proteomic levels, enabling the classification of EOC histological subtypes. Appl...

Purpose Superficial peritoneal endometriotic (pEM) lesions are composed of endometrial glands and stroma, in addition to a third component—myofibroblasts and smooth muscles (SM)-like cells. The latter develops secondary to a metaplasia. In this study, we characterised the third component cells in pEM according to differentiation markers in differen...

Abstract Background. The article gives an overview of the current diagnostics of ovarian cancer, a rare but often fatal disease. The most recent development in this field has shown that from the tumor biology, ovarian cancer is not one disease but different subtypes each with a specific pathogenesis,morphology, molecular pathology and prognosis. M...

Low-grade serous ovarian carcinoma (LGSOC) has recently come up as a distinct rare entity of epithelial ovarian cancer. Predictive and prognostic markers are not well studied yet. As Ki-67 and hormone receptors (HR) has been established as relevant cancer biomarkers in several malignant tumors we evaluated Ki-67 and HR expression rates by immunohis...

Objective Advanced‐stage ovarian high‐grade serous carcinoma (HGSC) is a poor‐prognosis cancer, however a small and poorly characterized subset of patients shows long‐term survival. We aimed to establish a cohort of HGSC long‐term survivors for histopathological and molecular analysis. Methods Paraffin blocks from 151 patients with primary FIGO II...

BACKGROUND: High-grade serous ovarian cancer (HGSOC) intratumoural vasculature evolution remains unknown. The study investigated changes in tumour microvessel density (MVD) in a large cohort of paired primary and recurrent HGSOC tissue samples and its impact on patients' clinico-pathological outcome. METHODS: A total of 222 primary (pOC) and r...

Background: High-grade serous ovarian cancer (HGSOC) intratumoural vasculature evolution remains unknown. The study investigated changes in tumour microvessel density (MVD) in a large cohort of paired primary and recurrent HGSOC tissue samples and its impact on patients' clinico-pathological outcome. Methods: A total of 222 primary (pOC) and rec...

Background: Interactions between cancer cells and the host immune system influence tumor biology, response to therapy and patient survival and their modulation offers promising new approaches for cancer therapy. The downregulation or loss of HLA class I expression in breast cancer cells might be an effective mechanism to evade the recognition by th...

Maligner Aszites beeinträchtigt die Lebensqualität von Krebspatienten gravierend. Aufgrund der oft palliativen Situation der Patienten ist eine kurative Therapie nicht immer möglich. Es gibt aber effektive Behandlungsmethoden, die auf neuen Forschungsergebnissen zur Pathophysiologie des malignen Aszites basieren.

Brenner tumors (BT) are rare ovarian tumors encompassing benign, borderline and malignant variants. While the histopathology of BTs and their clinical course is well described, little is known about the underlying genetic defects. We employed targeted next generation sequencing to analyze the mutational landscape in a cohort of 23 BT cases (17 beni...

Background: Pleomorphic rhabdomyosarcomas of the uterus, mainly occurring in postmenopausal women with leading symptoms of vaginal bleeding and abdominal pain, are very rare malignant tumors of the female genital tract. Due to the inefficiency of the adjuvant therapy, the outcome remains poor in the majority of the reported cases. Patient and met...

Background: LGSOC is a rare and distinct entity characterized by younger age, lower response to chemotherapy and better clinical outcome. Aim of this study was to evaluate the impact of Ki67, estrogen and progesterone receptors (ER and PR) on platinum response and survival in primary LGSOC patients. Methods: 80 primary LGSOC patients with available...

High-grade serous ovarian carcinoma remains one of the most lethal malignancies in females. For histopathological differentiation from mesothelioma cytokeratin, 5/6 immunohistochemistry is widely used. Another preferred marker for differential diagnosis to mesothelioma is ER-α. In this study, we determined the rate of cytokeratin 5/6 positive cells...

Background. Wilms tumor protein 1 (WT1) was first discovered as a tumor suppressor gene. Its expression is used in gynecological pathology as a diagnostic marker of serous differentiation, and is frequently co-expressed with ER-α. Because WT1 is not widely expressed in adult tissue it is a promising target for immunotherapy and early phase studies...

Background/aim: Neuroendocrine neoplasms (NEN) of the female genital tract account for 2% of gynecological cancers. The aim of this study was to share our experience of 11 primary neuroendocrine neoplasms of the ovary. Patients and methods: All patients who presented and/or were treated at our Institution with histologically-confirmed NEN of the...

STUDY HYPOTHESIS Loss of protein BAF250a (ARID1A) expression is present in women with rectovaginal deep-infiltrating endometriosis (DIE) and endometriosis affecting the pelvic sentinel lymph nodes (PSLN).

Aims: Wilms tumor protein 1 (WT1) expression is used in gynecological pathology as a diagnostic marker of serous differentiation, and is frequently co-expressed with ER-α. Early phase studies on WT1 vaccine in gynecological cancers are ongoing. In this study we aimed to determine the prognostic value of WT1 in high-grade serous ovarian carcinoma....

ESGE Congress Abstract Title: Can endometriotic cells spread through lymph nodes like cancer cells? Borrelli GM1, 2, Abrão MS2, Taube E3, Esfahani SD3, Chiantera V1, Mechsner S1 1Universitätsmedizin Berlin – Department of Gynaecology - Charité, Campus Benjamin Franklin, Germany 2University of Sao Paulo, Faculty of Medicine – Department of Obst...

Objective: To determine if ultrastructural tissue trauma occurs in the junctional zone in uteri in adenomyosis. Design: A case-control experimental study. Setting: Endometriosis research center. Patient(s): Twelve uteri with adenomyosis, and 9 uteri without adenomyosis, were gained during laparoscopy-assisted vaginal hysterectomy. Intervent...

We present the case of a Herpes simplex virus-1 (HSV-1) sepsis with severe herpes hepatitis in a young female treated with triple immunosuppressive therapy (adalimumab, azathioprine, prednisolone) for refractory Crohn's disease (CD). The patient presented with high fever, generalized abdominal tenderness, strongly elevated transaminases, coagulopat...

Endometriosis is a prevalent benign disease, despite sharing several similarities with malignancies, such as the possibility of lymphatic spread. In malignancies, chemokines play a sovereign role in the process of metastasis. Metastasis-related chemokine axes have not yet been assessed in deep-infiltrating endometriosis (DIE), and this investigatio...

Neuroendocrine differentiation in high-grade serous ovarian carcinomas has only rarely been described. However, in our consultancy experience, we have been pointed at a case of neuroendocrine relapse in a patient with high-grade serous ovarian carcinoma where retrospectively, a minor neuroendocrine component in the primary tumor could be detected....

Partial loss of BAF250a (ARID1A) in deep endometriosis and compromised pelvic sentinel lymph nodes: a normal phenomenon or an early event of malignant transformation in endometriosis? Borrelli GM1, 2, Abrão MS2, Taube E3, Esfahani SD3, Chiantera V1, Mechsner S1 1Universitätsmedizin Berlin – Department of Gynaecology - Charité, Campus Benjamin Fran...

Aims. Neuroendocrine differentiation in type 2 carcinomas has only seldom been described. However, in our consultancy experience we have been pointed at a case of neuroendocrine relapse in a patient with type 2 ovarian carcinoma where retrospectively a minor neuroendocrine component could be detected. Therefore we hypothesized that neuroendocrine d...

A 19-yr-old woman with previously diagnosed clear cell adenocarcinoma was referred to the Charité for further treatment. Biopsies were taken from the cervix, the endometrium, pseudomembranes in the peritoneum, and sentinel lymph nodes. The morphologic picture of pseudomembranes and inflammation together with the provided information about plasminog...