About
76
Publications
26,154
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,693
Citations
Current institution
Publications
Publications (76)
Sweet potato (SP) byproducts (rejected SP) and SP plant (leaves and stems; SPLS) can be used in animal feed, assuring nutritious and cost-effective diets. These byproducts have high nutritional value but must be preserved due to their high moisture content. This study aims to chemically and nutritionally characterize these byproducts preserved as s...
The objective of this work is to evaluate the influence of pomegranate peel extract (PPE) in the behavior of breast cell lines (epithelial and tumor type) and related oxidative metabolism. Fruit-based functional foods have been the target of increasing scientific research for their physiological and pathophysiological properties. Pomegranate (Punic...
Simple Summary
A growing consumer focus on animal welfare, high-quality meat, and product traceability has led to an increased demand for meat from ancient local breeds reared outdoors. Traditionally, these systems involve the slaughter of heavy and fat pigs, which are castrated to mitigate boar taint, an off odor and flavor rejected by consumers w...
Every year, considerable amounts of agro-industrial co-products are produced in Portugal that can be used in animal
feed, ensuring nutritious and low-cost diets. Sweet potato (SP), brewers’ grains (BG), and tomato pomace (TP) are
a few examples of co-products that have great nutritional value but must be preserved for usage outside of their
product...
Two groups of 8 individually housed young crossbred-bulls, in the finishing period, were used to test the effect of a Total Mixed Ration diet with high forage content (54% DM), low starch content (14% DM), supplemented with sunflower seeds (10% DM) (HFS) on growth performance, carcass and meat quality, fatty acid profile and carbon footprint, with...
A utilização de coprodutos agroindustriais na alimentação animal é uma oportunidade para reduzir as importações de matérias-primas, os desperdícios alimentares e transformar produtos sem valor em alimentos para animais, de alta qualidade e baixo custo e que não competem com a alimentação humana. Contudo, características dos coprodutos como sejam a...
Abstract
The use of agro-industrial by-products in animal feed is an opportunity to reduce imports, food waste and promote a clean and circular economy, turning worthless raw materials into high-quality and low-cost animal feeds, which does not compete with human food. This study aims to produce silages with by-products of carrot, sweet potato, pot...
Neste Estudo Fez-se a Avaliação de Três Misturas Forrageiras Produzidas na Herdade da Comenda (INIAV-Elvas), no Que se Refere à Produtividade e Qualidade Dessa Misturas para Utilizar na Produção de Bovinos de Carne.
A produção pecuária extensiva é um dos elementos principais dos sistemas agrários típicos das regiões mediterrânicas. Baseia-se no pas...
Ruminants host a complex anaerobic microbial ecosystem in the rumen that digests fiber efficiently but also isomerizes and hydrogenates extensively the dietary unsaturated fatty acids through biohydrogenation metabolic pathways (RBH). Generally, RBH yields t11-18:1 as the main intermediate, but when ruminants are fed high-starch diets tend to devel...
To test the hypothesis that lambs fed freeze-dried Nannochloropsis oceanica (NO) biomass will have a higher deposition of EPA in tissues than those fed other Nannochloropsis EPA-sources, we fed 28 lambs with one of four diets: i) C, control, without EPA; ii) O, with 1.2% Nannochloropsis oil; iii) SD, with 12.3% spray-dried NO biomass; iv) FD, with...
This study was carried out to test the hypothesis that the inclusion of condensed tannins (CT) from Cistus ladanifer aerial part or extract in a diet composed of dehydrated lucerne and supplemented with 60 g/kg of soybean oil can modulate the rumen microbiome and increase the production of healthy biohydrogenation intermediates (BI). Hence, thirty-...
Cistus ladanifer has been used in ruminants feeding systems, but there is no information about the seasonal variation in chemical composition and nutritional value of each morphological fraction during its growth cycle. This study characterized the morphological fractions of C. ladanifer (leaves, stems, flower buds, flowers, and seed heads) through...
Thirty-two bulls were assigned to four total mixed biodiverse haylage-based diets to evaluate the effects of partial substitution of grains by agroindustrial byproducts, sunflower seeds (SS) supplementation and haylage level on growth, in vitro methane production and carcass and meat quality. Dietary treatments included a grain-based diet with 30%...
Condensed tannins (CTs) are widely distributed in plants, and due to their recognized antioxidant activity are considered as possible natural antioxidants for application in ruminant diets. A wide range of CT-rich sources has been tested in ruminant diets, and their effects on animal antioxidant status and oxidative stability of their products are...
The effect of regulated deficit irrigation (RDI) strategies on fruit yield and quality at harvest and during cold storage at 5 °C of Wonderful pomegranates was studied. Three irrigation strategies were tested: control strategy (C) using farm irrigation with 100% of crop evaporation (ETc), and two RDI strategies where 25% of ETc was applied during f...
Simple Summary
Ruminant edible products have been associated with adverse health effects, due to their high saturated fatty acids and low polyunsaturated fatty acids content, resulting from the extensive biohydrogenation conducted by rumen microbiota. Cistus ladanifer condensed tannins were able to change the lamb ruminal biohydrogenation, increasi...
Cistus ladanifer L. starts to be used in the nutrition of ruminants due to its bioactive compounds, secondary metabolites and their dietary potential. C. ladanifer L. contains condensed tannins that have a high affinity for binding salivary proteins forming (in)soluble complexes. The induction of certain types of salivary proteins, by dietary tanni...
Utilization in ruminant diets of Cistus ladanifer (a shrub native from Mediterranean region) or its condensed tannin (CT) extracts has been intensively explored in the last years, with a wide range of studies that have provided valuable information on conditions of use, benefits and action mechanisms. Although it is considered as a feed with poor n...
Olive oil mill wastewater is a strong pollutant with low pH (4.63) and high salinity (2.70 dS m−1), organic matter (chemical oxygen demand (COD) = 7910 mg L−1), total phosphorus (1049.7 mg L−1), total phenols (540.4 mg L−1) and turbidity (280.1 NTU). This study provides the first application of precipitation processes using HCl, H2SO4, HNO3, NaOH a...
Cistus ladanifer L. is a shrub present in Mediterranean areas without major use, but which can be incorporated into ruminant diets, improving the digestive efficiency of dietary protein and product quality. The high levels of plant secondary metabolites, including condensed tannins, may be responsible for the beneficial properties of the plant, but...
Making cheese manufacturing environmentally sustainable is a major concern in the integrated management of this industrial sector. This concern is mainly due to the environmental impact of the discharge of its wastewaters, carrying heavy loads of salinity, nutrients, organic matter, solids and oils and fats. These discharges must meet increasingly...
Cistus ladanifer L. is a common shrub endemic to the Mediterranean with high levels of condensed tannins (CT). CT form complexes with dietary protein resisting microbial degradation in the rumen, which enhances dietary protein utilization in ruminant diets. The objective of this study was to evaluate the utilization of CT in the diet of lambs on th...
The treatment of cheesemaking wastewater by means of four processes (CaCO3, Mg(OH)2, FeSO4 and FeCl3) has been investigated in order to reduce the total phosphorus, turbidity, total phenols, chemical oxygen demand (COD) and absorbances. It has been demonstrated that CaCO3 is the most efficient process studied for total phosphorus removal, with redu...
The effect of Cistus ladanifer (rockrose) condensed tannins (CT) on dietary protein utilization in lambs was evaluated. The experiment was conducted to test the hypothesis that treating soybean meal (SBM) with rockrose condensed tannins will improve dietary protein utilization allowing a reduction of the dietary crude protein content of lamb's diet...
Cistus ladanifer L. is a shrub from Cistaceae family, widespread in Mediterranean countries. Fatty acids (FA) have multiple roles in plants and are involved in adaption mechanisms to environmental conditions. This work evaluated the FA content and composition of each morphological fraction of C. ladanifer (leaves, stems, flower buds, flowers and se...
Thirty-six lambs were used to evaluate the effect of three levels of Cistus ladanifer condensed tannins (CT; 0, 1.25 and 2.5%) and two ways of CT supply (C. ladanifer aerial parts and C. ladanifer CT extract) on lamb growth performance, carcass composition, meat quality and FA composition of intramuscular and subcutaneous fat. The basal diet was co...
The aim of this study was to evaluate the effect of the dietary inclusion of Cistus ladanifer L. and vegetable oils on intramuscular fat composition, muscle antioxidant status and lipid oxidative stability, and explore the mechanism by which dietary Cistus ladanifer limits the lipid oxidation. Lambs were assigned 9 diets, corresponding to 3 levels...
This study reports the first time that cheese whey wastewater has been treated by the hydrogen peroxide oxidation alone (uncatalytic reaction). This oxidation type was capable of eliminating the total phosphorus content in about 46–53%, regardless of the dose applied (1–7 g L⁻¹). For total phenols contents, the oxidation system was capable of reduc...
The performance of five innovative chemical precipitation processes (H2SO4, HNO3, HCl, Ca(OH)2 and NaOH) for the pretreatment of cheese wastewater was investigated.
For acid precipitation, a linear relationship between pH of the effluent and precipitation pH was found. High conductivity values were obtained when applying extreme pH conditions in ac...
Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP). The diets were supplemented with 6% soybean oil and included 40% o...
Thirty-two Merino lambs were used in experiment designed to evaluate the effects of forage particle size and starch level in complete mixed diets, on growth performance, carcass and meat quality traits. All the diets were supplemented with 6% soybean oil and include 40% of alfalfa. The lambs were randomly allocated to eight diets that combined two...
The long-term effect of thermal pasteurization (TP) and high-pressure processing (HPP) of orange juices stored under refrigeration, on the bioactive components and antioxidant activity, was compared. Total phenolic content (TPC), flavonoid, anthocyanin, and carotenoid contents, the individual content of major phenolic components, and the antioxidan...
With this trial we have tested the effects of structural and chemical composition of neutral detergent fibre (NDF) of the diet on lamb fatty acid composition of meat and subcutaneous fat. Twenty lambs, were fed complete diets with low starch and similar NDF content of different origin (ground alfalfa or soybean hulls). Animal performance and produc...
Cynara cardunculus: L. represents a natural source of terpenic compounds, with the predominant molecule being cynaropicrin. Cynaropicrin is gaining interest since it has been correlated to anti-hyperlipidaemia, antispasmodic and cytotoxicity activity against leukocyte cancer cells. The objective of this work was to screen a collection of C. cardunc...
Alentejano pig is an autochthonous breed from the south of Portugal, traditionally finished under free-range conditions (Montanheira). Meat and fat of this breed are used to manufacture high quality meat products. Despite the impact on final product quality and on productive efficiency, carcass and meat quality of pigs available for manufacture of...
Meat from lambs finished with high-starch diets often contains low concentration of vaccenic (t11-18:1) and rumenic (c9,t11-18:2) acids and high concentration of t10-18:1. We hypothesized that replacing cereals by dehydrated citrus pulp (DCP) and the inclusion of tanniferous feed sources in oil supplemented diets might reduce the accumulation of t1...
In this study we measured 13C values of the main fatty acids (FA) present in neutral and polar intramuscular lipids of meat samples from 24 lambs, fed with four different diets supplemented with sunflower and linseed oil and the tanniferous shrub Cistus ladanifer L. The objective was to understand if the increase in intramuscular fat observed in l...
Background
Cynara cardunculus L. offers a natural source of phenolic compounds with the predominant molecule being chlorogenic acid. Chlorogenic acid is gaining interest due to its involvement in various biological properties such as, antibacterial, antifungal, antioxidant, hepatoprotective, and anticarcinogenic activities. ResultsIn this work we s...
Cheese constitutes an important source of essential nutrients and health-promoting compounds in human diet, including proteins, amino acids, bioactive peptides, fatty acids, minerals, vitamins, and polyphenolic compounds. Cheese nutritional value depends on the milk characteristics and the cheesemaking conditions, determining unique and distinct nu...
Cistus ladanifer (rockrose) is a tanniferous and aromatic shrub abundant in marginal fields of Mediterranean countries that when fed to lambs has been associated with changes in rumen biohydrogenation that led to an increased concentration of vaccenic acid (t11-18:1) in abomasal digesta and meat. It is not clear what type of secondary compounds pre...
The effects of feeding Cistus ladanifer (Cistus) and a blend of soybean and linseed oil (1 : 2 vol/vol) on fatty acid (FA) composition of lamb meat lipids and messenger RNA (mRNA) expression of desaturase enzymes was assessed. In total, 54 male lambs were randomly assigned to 18 pens and to nine diets, resulting from the combination of three inclus...
Tannins are widespread throughout the plant kingdom, occurring as hydrolysable and condensed tannins and at different levels in several animal feeding sources. Recent years have seen an increasing interest in the use of tannin-rich plants and plant extracts in ruminant diets for improving the quality of their edible products. Some results show that...
Tannins are widespread throughout the plant kingdom, occurring as hydrolysable and condensed tannins and at different levels in several animal feeding sources. Recent years have seen an increasing interest in the use of tannin-rich plants and plant extracts in ruminant diets for improving the quality of their edible products. Some results show that...
Shrubs can provide an important contribution to the fodder resources for small ruminants in Mediterranean areas, but there is limited information on their feed value, including secondary metabolites and their seasonal differences. This study evaluated the effect of seasonal variation in chemical composition, in vitro digestibility and antioxidant a...
The Cistus ladanifer L. shrub is a widespread species of the Mediterranean region that is available as a feed source for ruminants all the year round, constituting a source of energy and nutrients when most of the vegetation is dry. However, there is no trustworthy information about the fatty acid composition of C. ladanifer, as well as no informat...
Sensory characteristics of cheeses constitute probably the most important attributes driving consumers’ choice. Prior to and during ingestion itself, the consumer can perceive several sensory features of cheese, which are generally grouped under appearance, flavour and texture. All such attributes determine the eating quality of cheeses and consequ...
The aims of the study were: (i) to investigate effects of a low protein diet on fatty acids content and composition of the longissimus muscle and subcutaneous adipose tissue in three genetically diverse breeds: Large White × Landrace, Alentejano, and Bizaro; (ii) to determine whether the effect of the low protein diet of fatty acid composition is a...
Phenolic composition, antioxidant and breast cancer antiproliferative activities of water and methanol/water derived extracts from olive pomace (OP) and dry olive mill residue (DOR), from Portuguese industries, were studied. DOR water (DORW) extracts showed the highest extraction yield; as well as the highest total phenolic content (TPC) and hydrox...
The potential use of odd- and branched-chain fatty acids (OBFA) and purine bases (PB) as microbial markers in the equine hindgut was studied. For this purpose, feed particles adherent bacteria (solid associated bacteria, SAB) and planktonic bacteria (liquid associated bacteria, PAB) were isolated from total cecum and colon contents of 8 healthy, cr...
This experiment was conducted to test the hypothesis that adding glucogenic precursors (propylene glycol and calcium propionate mix) to a high-forage and high-oil diet would enrich lamb meat in cis-9,trans-11
18:2 (rumenic acid), through both the maintenance of high level of trans-11 18:1 in rumen outflow and an increase in its endogenous conversio...
The effect of replacement of dietary sunflower oil (SO) with linseed oil (LO) on Stearoyl CoA desaturase (SCD) gene expression was investigated. Thirty-six lambs were randomly assigned to four groups and fed with one of the experimental diets, consisting of lucerne pellets with oil (60 g/Kg). The diets varied in the percentage of the oil supplement...
The deposition of C18 fatty acids (FA), especially in rumen biohydrogenation intermediates, was studied using 36 lambs fed four diets with graded proportions of sunflower oil (SO) and linseed oil (LO). Lambs were fed one of four diets consisting on dehydrated lucerne with either: 6% SO, 4% SO plus 2% LO, 2% SO plus 4% LO and 6% LO. The profile of C...
We hypothesized that reducing weight properties of conjugated linoleic acid (CLA) are due to adipocyte apoptosis and that CLA differentially modulates the apoptotic responses in hepatic lipotoxicity from rats fed saturated fat diets. Obese Zucker rats were fed atherogenic diets (2%w/w of cholesterol) formulated with high (15%w/w) saturated fat, fro...
Two experiments were conducted using laboratory mini-silos to study the effect of ensiling and silage additives on fatty acid (FA) composition, including minor or unusual FA, of ryegrass and corn silages. Ryegrass was ensiled for 12 wk with no additives, with the addition of a bacterial inoculant or formic acid. Corn was ensiled for 9 wk without ad...
Thirty-six lambs were used in a 6 week experiment to evaluate the effect of vegetable oil blend supplementation (0 vs 60 g/kg of dry matter (DM)) and two dietary condensed tannin sources, grape seed extract (0 vs 25 g/kg of DM) and Cistus ladanifer L. (0 vs 250 g/kg of DM), on fatty acid (FA) composition of abomasal digesta and intramuscular polar...
Thirty-six Merino Branco lambs were assigned to six dietary treatments: control diet (C) consisting of 90% dehydrated lucerne and 10% wheat bran; control diet with 6% of oil blend (CO); control with 2.5% of grape seed extract (GS); control with 2.5% of grape seed extract and 6% of oil blend (GSO); control with 25% of Cistus ladanifer (an aromatic b...
The effect of stepwise replacement of dietary sunflower oil (SO) with linseed oil (LO) on carcass composition, meat colour and fatty acid (FA) composition of intramuscular lipids of lamb meat was investigated. Thirty-six lambs were fed one of four diets consisting of pellets of lucerne with oil (60g/kg): the diet varied in the composition of oil ad...
This study sought to distinguish liquid-(LAB) and detached (SAB1) and undetached (SAB2) solid-associated bacteria through their fatty acid (FA) and purine base (PB) profiles. Fatty acids and PB were also evaluated as internal microbial markers for estimating microbial biomass associated with rumen particles. Four merino rams fitted with rumen cannu...
The objective was to characterize the fatty acid (FA) composition of lamb meat with emphasis on biohydrogenation intermediates (BI) induced by dietary sunflower and linseed oil and to test if a synergistic effect on meat trans-11 18:1 and cis-9,trans-11 18:2 concentrations could be obtained with a blend of both oils. Thirty two lambs were assigned...
