Eliana B. Souto

Eliana B. Souto
University of Porto | UP

Dr. habil., HDR, PhD

About

556
Publications
162,015
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
17,322
Citations

Publications

Publications (556)
Article
Full-text available
Over the last decades, we have witnessed an increasing interest in food-related products containing vegetable oils. These oils can be obtained either by extraction or by mechanical pressing of different parts of plants (e.g., seeds, fruit, and drupels). Producers of nutraceuticals have ceaselessly searched for unique and effective natural ingredien...
Article
Full-text available
Biosensor-integrated drug delivery systems are innovative devices in the health area, enabling continuous monitoring and drug administration. The use of smart polymer, bioMEMS, and electrochemical sensors have been extensively studied for these systems, especially for chronic diseases such as diabetes mellitus, cancer and cardiovascular diseases as...
Article
Full-text available
Formononetin suppresses catabolic effects in primary rat chondrocytes induced by IL-1β, which makes it a promising candidate for in vivo studies on the treatment and prevention of osteoarthritis (OA). The goal of this study is to investigate the effects of the oral administration of formononetin in a rodent model of OA. OA was induced by medial men...
Article
Full-text available
Hydrogels are a great ally in the pharmaceutical and biomedical areas. They have a three-dimensional polymeric structure that allows the swelling of aqueous fluids, acting as an absorbent, or encapsulating bioactive agents for controlled drug release. Interestingly, plants are a source of biogels, specifically polysaccharides, composed of sugar mon...
Article
Full-text available
Nutraceuticals have gained increasing attention over the last years due to their potential value as therapeutic compounds formulated from natural sources. For instance, there is a wide range of literature about the cardioprotective properties of omega-3 lipids and the antioxidant value of some phenolic compounds, which are related to antitumoral ac...
Article
Full-text available
Non-melanoma carcinoma has high incidence rates and has two most common subtypes: basal cell carcinoma and squamous cell carcinoma. This type of carcinoma is usually not fatal; however, it can destroy sensory organs such as the nose, ears, and lips. The treatment of these injuries using non-invasive methods is thus strongly recommended. Some treatm...
Article
Full-text available
This work deals with the creation of new multifunctional quaternary ammonium compounds (QACs) on natural isatin scaffold as a new generation of antimicrobial agents. 1-R-isatin-3-acylhydrazones containing quaternized DABCO moieties (Dabco-Is-n) with varying hydrophobicity (R = CnH2n+1, where n = 10, 12, 14, 16, 18) were synthesized. The idea was to...
Article
Full-text available
In this work, three pesticides of different physicochemical properties: glyphosate (GLY, herbicide), imidacloprid (IMD, insecticide), and imazalil (IMZ, fungicide), were selected to assess their cytotoxicity against Caco-2 and HepG2 cells. Cell viability was assessed by the Alamar Blue assay, after 24 and 48 h exposure to different concentrations,...
Article
Full-text available
The increased mortality rates associated with antibiotic resistance has become a significant public health problem worldwide. Living beings produce a variety of endogenous compounds to defend themselves against exogenous pathogens. The knowledge of these endogenous compounds may contribute to the development of improved bioactive ingredients with a...
Article
Full-text available
Endodontic-related diseases constitute the fourth most expensive pathologies in industrialized countries. Specifically, endodontics is the part of dentistry focused on treating disorders of the dental pulp and its consequences. In order to treat these problems, especially endodontic infections, dental barriers and complex root canal anatomy should...
Article
Full-text available
Nano-hybrid systems are products of interactions between organic and inorganic materials designed and planned to develop drug delivery platforms that can be self-assembled. Poloxamine, commercially available as Tetronic®, is formed by blocks of copolymers consisting of poly (ethylene oxide) (PEO) and poly (propylene oxide) (PPO) units arranged in a...
Article
Full-text available
Natural polysaccharides are structures composed of highly diversified biological macromolecules whose properties have been exploited by a diversity of industries. Until 2018, the polysaccharides market raised more than US $ 12 billion worldwide, while an annual growth forecast of 4.8% is expected by 2026. The food industry is largely responsible fo...
Article
Full-text available
In this work, three pesticides of different physicochemical properties, namely, glyphosate (herbicide), imidacloprid (insecticide) and imazalil (fungicide), were selected to assess their cytotoxicity against distinct cell models (Caco-2, HepG2, A431, HaCaT, SK-MEL-5 and RAW 264.7 cells) to mimic gastrointestinal and skin exposure with potential sys...
Article
Full-text available
Food color is often associated in the collective imagination to properties like flavor, taste, sensory qualities, and genuinity. While synthetic food dyes may cause several harmful effects to human health, and most of them are not used or allowed in food manufacturing according to the regulations of each country, the origin of food dyes are receivi...
Article
Endoscopic submucosal dissection (ESD) and endoscopic submucosal resection (EMR) are non-invasive endoscopic techniques. They allow an early excised gastrointestinal (GI) mucosal precancerous lessions. For their application is necessary use of a submucosal injection that lift area to excise. The main objective of this study was the preparation of m...
Article
Full-text available
Melanoma is a complex type of cancer that depends on several metabolic factors, while the currently used therapies are not always effective and have unwanted side effects. In this review, the main factors involved in the etiology of cutaneous carcinoma are highlighted, together with the main genes and proteins that regulate cancer invasion and meta...
Article
Obesity is a prevalent public health concern in several countries, and is closely associated with several pathological disorders, including diabetes, hypertension, cardiovascular diseases, and increased dyslipidemia. Dyslipidemia is an asymptomatic condition characterized by high levels of low-density lipoproteins (LDL) and low levels of high-densi...
Article
The widespread use of Brazilian red propolis extract has promoted an increased production rate in the Northeast region of Brazil, with an impressive economic turnover for the region. The rational use of the hydroalcoholic extract of red propolis (HERP) raised the question about its value against obesity. In this work, we aimed at evaluating the ant...
Article
Customized cationic oil-in-water nanoemulsions (NEs) have been produced to improve the bioavailability of poorly water-soluble drugs, such as triamcinolone acetonide (TA). TA is a synthetic glucocorticoid with anti-inflammatory and antiangiogenic therapeutic properties and it is widely used as an effective treatment in ocular disorders. In this wor...
Article
Full-text available
Lipid nanoparticles are currently used to deliver drugs to specific sites in the body, known as targeted therapy. Conjugates of lipids and drugs to produce drug-enriched phospholipid micelles have been proposed to increase the lipophilic character of drugs to overcome biological barriers. However, their applicability at the topical level is still m...
Article
Full-text available
Innate and adaptive immunity are essential for neurodevelopment and central nervous system (CNS) homeostasis; however, the fragile equilibrium between immune and brain cells can be disturbed by any immune dysregulation and cause detrimental effects. Accumulating evidence indicates that, despite the blood–brain barrier (BBB), overactivation of the i...
Article
Nanometrology has been considered as a key to the future of nanotechnology since recently. It is particularly important for nanoparticle market development that is commonly applied in dif-ferent areas of science. The adoption of nanotechnologies in food and agrochemical industries represents a new frontier, with particular regard to plant defence a...
Article
In recent years, nanotechnology has allowed to formulate different ophthalmic vehicles to extend residence time in the eye after topical application, decreasing the number of instillations and improving patient's compliance. The goal of this study was to develop an oil-in-water (O/W) nanoemulsions (NEs), composed of almond oil as the inner oil phas...
Article
Full-text available
Lactide dimer is an important monomer produced from lactic acid dehydration, followed by the prepolymer depolymerization process, and subsequent purification. As lactic acid is a chiral molecule, lactide can exist in three isomeric forms: L-, D-, and meso-lactide. Due to its time-consuming synthesis and the need for strict temperature and pressure...
Article
Full-text available
Microemulsions and nanoemulsions are lipid-based pharmaceutical systems with a high potential to increase the permeation of drugs through the skin. Although being isotropic dispersions of two nonmiscible liquids (oil and water), significant differences are encountered between microemulsions and nanoemulsions. Microemulsions are thermodynamically st...
Article
Scientific research in the field of veterinary pharmacology has provided new opportunities for the development of modified release dosage forms, with the aim to improve therapeutic efficacy and reduce animal stress. The formulation of classical drug molecules with advanced biomaterials has become a new approach to increase drug bioavailability and...
Article
Full-text available
The potato is a root vegetable native to the Americas; it consists of the starchy tuber of the plant Solanum tuberosum. There are many varieties, and the flesh can have different colour ranging from yellow to red and purple. Coloured varieties have a denser texture and slightly nuttier, earthier flavour than other potatoes. The desirable quality ch...
Article
Full-text available
Lycopene intake from tomatoes and other food sources has multiple potential health benefits. This report aimed to evaluate the current research literature on lycopene concerning human and animal health. The electronic Web of Science Core Collection database was searched with (lycopene*) AND (health* OR illness* OR disease* OR medic* OR pharma* OR d...
Article
Medicinal plants have always been prescribed around the world with therapeutic purposes for various diseases and disorders related to their profile of biologically active substances and health-promoting effects. Meantime, Rhodiola rosea has been of particular importance among physicians, researchers and the general public. Accordingly, the present...
Article
Full-text available
Age-related macular degeneration (AMD) is an eye disease typically associated with the aging and can be classified into two types—namely, the exudative and the nonexudative AMD. Currently available treatments for exudative AMD use intravitreal injections, which are associated with high risk of infection that can lead to endophthalmitis, while no su...
Article
Full-text available
The use of natural products in sunscreen formulations as a prophylactic measure against skin cancer is receiving special attention attributed to the photoprotective and antioxidant properties of their chemical components. In this work, we describe the development of topical hydrogel formulations containing hydroalcoholic extract of red propolis (HE...
Article
Full-text available
Introduction Resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a polyphenol obtained from diverse groups of plants, especially in the muscadine grape, red wine, lingonberry, cranberry and redcurrant. Resveratrol's health benefits were first highlighted in the study of the French paradox, which opened up an expansive research endeavor into this comp...
Article
Full-text available
The skin is a complex and multifunctional organ, in which the static versus dynamic balance is responsible for its constant adaptation to variations in the external environment that is continuously exposed. One of the most important functions of the skin is its ability to act as a protective barrier, against the entry of foreign substances and agai...
Article
Age-related macular degeneration (AMD) is defined as a degenerative, progressive and multifactorial disorder that affects the macula with a complex etiology. The retinal pigment epithelium is a monolayer of cells that has the function to separate the surface of the choroid from the neural retina that is involved in the signal transduction leading t...
Article
Full-text available
Despite the health benefits of the sun, overexposure to solar radiation without proper precautions can cause irreversible damage to exposed skin. In the search for balance between the risks and benefits of exposure to solar radiation in human health, a technological alternative was found, the incorporation of photoprotective products in lipid nanop...
Article
Full-text available
Biological activity of essential oils (EOs) has been extensively reported; however, their low aqueous solubility, high photosensitivity, and volatility compromise a broad industrial use of these compounds. To overcome these limitations, we proposed a nanoencapsulation approach to protect EOs, that aims to increase their stability and modulate their...
Chapter
By definition, drug targeting and delivery is the process of delivering a drug molecule to a specific site where the pharmacological effect is desired. A targeted release may simplify the drug administration protocols and reduce the amount of drug necessary, thus improving the cost-effectiveness and reducing the side effects. A drug administered by...
Chapter
The first evidence of vaccine development dates back to the year 1000 in ancient China. Since then, highly efficient and increasingly comprehensive vaccines have reached the market, involving technologies that are constantly developing and improving. Among these, DNA vaccines occupy a prominent place because they stimulate the cellular and humoral...
Chapter
The administration of bioactive molecules via the transdermal route has been studied by a large number of researchers in recent years. Several strategies are being employed to improve the transport of bioactive molecules through the skin, such as the use of physical methods such as (1) sonophoresis, (2) iontophoresis, (3) electroporation, (4) magne...
Article
Full-text available
This review highlights the application of lipid nanoparticles (Solid Lipid Nanoparticles, Nanostructured Lipid Carriers, or Lipid Drug Conjugates) as effective drug carriers for pathologies affecting the posterior ocular segment. Eye anatomy and the most relevant diseases affecting the posterior segment will be summarized. Moreover, preparation met...
Article
Hydrogels for the modified-release drug delivery systems is a continuously growing area of interest for the pharmaceutical industry. According to the global market, the use of polymers in this area is projected to reach $31.4 million by 2027. This review discusses the recent advances and perspectives of hydrogel in drug delivery systems for oral, p...
Chapter
Consumer interest and demand are increasing worldwide and this is concentrated more towards functional foods, food supplements, and nutraceuticals containing micronutrients with health-promoting effects. This chapter is dedicated to giving an updated picture on nutraceuticals and functional beverages- in particular with a focus on beverages contain...
Article
Full-text available
The eye is a very complex organ comprising several physiological and physical barriers that compromise drug absorption into deeper layers. Nanoemulsions are promising delivery systems to be used in ocular drug delivery due to their innumerous advantages, such as high retention time onto the site of application and the modified release profile of lo...
Article
Full-text available
Spinal Cord Injury (SCI) promotes a cascade of inflammatory events that are responsible for neuronal death and glial scar formation at the site of the injury, hindering tissue neuroregeneration. Among the main approaches for the treatment of SCI, the use of biomaterials, especially gelatin methacryloyl (GelMA), has been proposed because it is bioco...
Article
Entomopathogenic fungus belonging to the genus Aschersonia [Sordariomycetes: Hypocreales: Clavicipitaceae] is known for its high virulence against Aleyrodidae and Coccoidea. Its first use as a biological control agent date back to 1921 in Florida, when it was reported to be active against citrus whiteflies. Despite its recognized effectiveness, aft...
Article
Full-text available
Epilepsy is the second most prevalent neurological disease worldwide. It is mainly characterized by an electrical abnormal activity in different brain regions. The massive entrance of Ca2+ into neurons is the main neurotoxic process that lead to cell death and finally to neurodegeneration. Although there are a huge number of antiseizure medications...
Article
Riluzole-loaded PLGA nanoparticles (RLZ-NPs) were developed to improve the biopharmaceutical profile of RLZ after ocular administration. Moreover, RLZ-NPs were dispersed in an in situ gelling system (RLZ-NPs-Gel) for topical administration as a potential neuroprotective strategy against glaucoma. Formulations were optimized using the design of expe...
Article
Full-text available
Plant species are fundamental source of nectar in beekeeping since bees access nectar and pollen from flowers. Consequently, bee products are strongly linked to the bee foraging flora source, and, depending on this, they acquire defined features, including their health and medicinal properties. Medicinal plants contribute greatly to increase the be...
Article
Full-text available
Hyperproliferative skin diseases (HSD) are a group of diseases that include cancers, pre-cancerous lesions and diseases of unknown etiology that present different skin manifestations in terms of the degree and distribution of the injuries. Anti-proliferative agents used to treat these diseases are so diverse, including 5-aminolevulinic acid, 5-fluo...
Article
Full-text available
Leishmania is a complex disease caused by the protozoan parasites and transmitted by female phlebotomine sandfly. The disease affects some of the poorest people on earth with an estimated 700,000 to 1 million new cases annually. The current treatment for leishmaniasis is toxic, long, and limited, in view of the high resistance rate presented by the...
Article
Full-text available
Nanocomposites as drug delivery systems (e.g., metal nanoparticles) are being exploited for several applications in the biomedical field, from therapeutics to diagnostics. Green nanocomposites stand for nanoparticles of biocompatible, biodegradable and non-toxic profiles. When using metal nanoparticles for drug delivery, the question of how hazardo...
Article
Full-text available
Despite significant advances in therapeutic possibilities for the treatment of inflammatory bowel disease (IBD) in recent years, there is still a big room for improvement. In particular, biological treatment can induce not only clinical remission but also mucosal healing of the gastrointestinal tract. Among these therapeutic molecules, anti-tumor n...
Article
Full-text available
The by-products/wastes from agro-food and in particular the fruit industry represents from one side an issue since they cannot be disposed as such for their impact on the environment but they need to be treated as a waste. However, on the other side, they are a source of bioactive healthy useful compounds which can be recovered and be the starting...
Article
Full-text available
Epilepsy is a chronic disease of the central nervous system characterized by an electrical imbalance in neurons. It is the second most prevalent neurological disease, with 50 million people affected around the world, and 30% of all epilepsies do not respond to available treatments. Currently, the main hypothesis about the molecular processes that t...
Article
Full-text available
Oil-in-water nanoemulsions (NEs) are considered a suitable nanotechnological approach to improve the eye-related bioavailability of lipophilic drugs. The potential of cationic NEs is prominent due to the electrostatic interaction that occurs between the positively charged droplets with the negatively charged mucins present in the tear film. This in...
Article
Full-text available
Dry eye disease (DED) is a high prevalent multifactorial disease characterized by a lack of homeostasis of the tear film which causes ocular surface inflammation, soreness, and visual disturbance. Conventional ophthalmic treatments present limitations such as low bioavailability and side effects. Lactoferrin (LF) constitutes a promising therapeutic...
Article
Bacteria can produce nitrogenous compounds via both primary and secondary metabolic processes. Many bacterial volatile nitrogenous compounds produced during the secondary metabolism have been identified and reported for their antioxidant, antibacterial, antifungal, algicidal and antitumor activities. The production of these nitrogenous compounds de...
Article
Full-text available
Quaternary derivatives of 1,4-diazabicyclo[2.2.2]octane (DABCO) and of quinuclidine surfactants were used to develop oil-in-water nanoemulsions with the purpose of selecting the best long-term stable nanoemulsion for the ocular administration of triamcinolone acetonide (TA). The combination of the best physicochemical properties (i.e., mean droplet...