Eleonora Cariolato

Galway-Mayo Institute of Technology | GMIT · Marine and Freshwater Research Centre

Hi,

I want to include the global Fis index in my divBasic calculation from the package diveRsity. To do so I set the bootstrap = 100.

However, I have this error in return:

Error in x[, 1] : incorrect number of dimensions

When I run the divBasic without the bootstrap I manage to obtain the correct output excel file; therefore, I believe that the error pop-up because of the bootstrap but I don't understand why.

My input file is a genepop file (.gen) with 82 SNPs marker (gp=3), 724 individuals from 28 different populations.

Does anyone have this issue before or have any idea on how to run the divBasic function with bootstrap to obtain Fis index?

Thank you very much in advance,

Best

Eleonora

I'm planning an experimental design to investigate the gene expression of mussels under thermal stress conditions, to compare their responses.

The experiment includes a control tank and a treatment tank, with enough mussels to sample 4 time points (60 mussels per time point per treatment).

In our facilities, I have two separate recirculation systems (with heaters and coolers) with two tanks each.

Since the two tanks of one recirculating system are connected they cannot be considered as isolated tanks and therefore they are not replicates. I'm planning to distribute the control samples between the two tanks of system 1 and the treatment samples between the two tanks of system 2, and at each time point sample from both tanks of one system and pooling them together (e.g. 30 samples from tank 1 and 30 samples from tank 2 of the control system)

My question is: do I need to have tank replicates for this experiment? Or can I sample three groups of 60 mussels per timepoint and per treatment between the two tanks of one system? i.e. have sample replicates within the control temperature and the treatment temperature tanks at each timepoint?

Thanks!