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16
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Introduction
Skills and Expertise
Current institution
Additional affiliations
April 2022 - present
April 2022 - present
Education
September 2007 - June 2013
Publications
Publications (16)
Mass spectrometry (MS) and proteomics are continuously evolving methods in systems biology that carry enormous potential for accurately profiling the proteome of diseased tissues and exploring biomarker molecules. In Huntington’s disease (HD), the identification of novel effective biomarkers for monitoring the disease progression from the early asy...
Driven by co-evolution with pathogens, host immunity continuously adapts to optimize defence against pathogens within a given environment. Recent advances in genetics, genomics and transcriptomics have enabled a more detailed investigation into how immunogenetic variation shapes the diversity of immune responses seen across domestic and wild animal...
In vertebrates, cannabinoids modulate neuroimmune interactions through two cannabinoid receptors (CNRs) conservatively expressed in the brain (CNR1, syn. CB1) and in the periphery (CNR2, syn. CB2). Our comparative genomic analysis indicates several evolutionary losses in the CNR2 gene that is involved in immune regulation. Notably, we show that the...
Cerebrospinal fluid (CSF) proteins regulate neurogenesis, brain homeostasis and participate in signalling during neuroinflammation. Even though birds represent valuable models for constitutive adult neurogenesis, current proteomic studies of the avian CSF are limited to chicken embryos. Here we use liquid chromatography–tandem mass spectrometry (nL...
In this study, we tested a method for long-term storage of oral mucosal epithelial cells (OMECs) so that the cells could be expanded in vitro after cryopreservation and used for the treatment of bilateral limbal stem cell deficiency. The ability of suspended primary OMECs to proliferate in vitro after cryopreservation was compared to that of OMEC c...
Background
Huntington’s disease (HD) is a hereditary neurodegeneration, caused by the accumulation of mutant huntingtin protein (mHTT) in brain, and particularly in striatum. mHTT protein aggregates and causes widespread disruption of cellular processes, including proteostasis.
Aims
To identify changes in the striatal proteome related to mHTT pres...
Ultrathin electrospun poly (l-lactide-co-dl-lactide) nanofibrous membranes coated with fibronectin were explored as scaffolds for the ex vivo cultivation of limbal epithelial cells (LECs) for the treatment of limbal stem cell deficiency. The developed scaffolds were compared with the “gold-standard” fibrin gel. The resulting membranes composed of n...
Adult neurogenesis is the life-long process of neural stem cell proliferation, differentiation into neurons, migration, and incorporation into the existing neuronal circuits. After decades of research, it is now widely accepted that mammals and birds retain the capacity to regenerate neurons even after their subadult ontogeny. Cerebrospinal fluid p...
In vertebrates, an ancient duplication in the genes for cannabinoid receptors (CNRs) allowed the evolution of specialised endocannabinoid receptors expressed in the brain (CNR1) and the periphery (CNR2). While dominantly conserved throughout vertebrate phylogeny, our comparative genomic analysis suggests that certain taxa may have lost either the C...
Cerebrospinal fluid (CSF) proteins regulate neurogenesis, brain homeostasis and participate in signalling during neuroinflammation. Even though birds represent valuable models for constitutive adult neurogenesis, current proteomic studies of the avian CSF are limited to chicken embryos. Here we use liquid chromatography–tandem mass spectrometry (nL...
Autonomic neuropathy is a complication of diabetes and, where the innervation of the gut is involved, results in disordered gut motility. In vivo, sympathetic nerves and subpopulations of enteric neurons supplying the ileum are differentially affected by diabetes. The overall of aim of this study was to establish whether such differential susceptib...
Diabetes has a differential effect on different subpopulations of myenteric neurons. Our aim was to investigate an in vitro model to examine the pathways underlying the development of nerve changes in diabetes.
The proportions of neuronal cell bodies containing vasoactive intestinal polypeptide (VIP), neuronal nitric oxide synthase (nNOS) and calbi...
Amyotrophic Lateral Sclerosis is a devastating neurodegenerative disease that affects motor neurons. Many theories about mechanisms contributing in its pathogenesis have been suggested. There is also evidence for alterations in synaptic plasticity during the progress of ALS. In particular, the expression of axonal growth promoting molecules like GA...
