Elena Zharkikh

Orlov State University · Research and Development Center of Biomedical Photonics

Timely diagnostics of microcirculatory system abnormalities, which are the most severe diabetic complications, is one of the major problems facing modern health care. Functional abnormalities manifest themselves earlier than the structural ones, and therefore their assessment is the issue of primary importance. In this study Laser Doppler flowmetry...

According to the International Diabetes Federation, the challenge of early stage diagnosis and treatment effectiveness monitoring in diabetes is currently one of the highest priorities in modern healthcare. The potential of combined measurements of skin fluorescence and blood perfusion by the laser Doppler flowmetry method in diagnostics of low lim...

In this study, we demonstrate what kind of relative alterations can be expected in average perfusion and blood flow oscillations during postural changes being measured in the skin of limbs and on the brow of the forehead by wearable laser Doppler flowmetry (LDF) sensors. The aims of the study were to evaluate the dynamics of cutaneous blood perfusi...

The prevalence of diabetes complications is a significant public health problem with a considerable economic cost. Thus, the timely diagnosis of complications and prevention of their development will contribute to increasing the length and quality of patient life, and reducing the economic costs of their treatment. This article aims to review the c...

The paper is devoted to the study of perfusion and amplitude-frequency spectra of laser Doppler flowmetry (LDF) signals in patients with diabetes mellitus (DM) in different skin areas of the upper and lower extremities using a distributed system of wear-able LDF analysers. LDF measurements were performed in the areas of the fingers, toes, wrists an...

This paper discusses a method for the diagnosis of peripheral blood flow disorders in type 2 diabetes mellitus (DM) using a distributed system of wearable blood microcirculation analyzers based on laser Doppler flowmetry. This method was tested in a clinical setting. The research resulted in the development of a classification model based on linear...

Significance The study of the effect of aging on the optical properties of biological tissues, in particular polarization, is important in the development of new diagnostic approaches. Aim This work aims to provide a comprehensive analysis of the factors and mechanisms that contribute to the alteration of skin polarization properties caused by agi...

The paper reports the results of blood microcirculation studies in microgravity using portable laser analyzer LAZMA PF. Two cosmonauts (C-1 and C-2) performed pre-launch and inflight (9 days) 8-minute sessions with the use of 2 portable flowmeters symmetrically attached to the temporal skin, palmer sides of distal phalanxes III, forearm superficie...

Performing yoga exercises in addition to basic training helps athletes to improve their results. At the same time, yoga exercises, including Hatha yoga, which involves controlling one’s breathing, are integrated into the training process. This work is devoted to study the influence of breathing exercises, with the decrease and increase in the minut...

The aim of this work was to study the regulation of insulin production by synucleins. In addition, we aimed to evaluate the possible efect of 1267 nm light irradiation, which leads to singlet oxygen production, on insulin production in the animal organism. the present work showed that knockout of genes encoding synuclein proteins is associated with...

Involvement of the blood microcirculation system in many functions of the body leads to the fact that the microcirculatory bed is one of the first links in the pathogenesis of various diseases, such as diabetes mellitus (DM). The aim of the present work was to improve the quality of diagnostics of peripheral blood flow disorders by developing a dia...

Currently, there is a surge of interest in wearable diagnostic devices with the possibility of daily monitoring of the functional state of the human body, for example, microcirculatory-tissue systems. The first such development is the «LAZMA PF» analyzer ((LAZMA Ltd, Russia; in EU/UK this device is produced by Aston Medical Technology Ltd., UK as «...

The work is devoted to the study of the effect of complete hyper- and hypoventilation breathing on the parameters of the blood flow microcirculation. Volunteers achieved hypo- and hyperventilation modes by performing full breathing. The correlations found between parameters of gas analysis and blood microcirculation may indicate the peculiarities o...

The paper is aimed at comparing blood microcirculation parameters of conditionally healthy volunteers and patients undergoing rehabilitation after COVID-19 to identify possible blood flow dysregulation that may result from the disease. A system of wearable laser Doppler monitors was used to conduct the study. The study demonstrated an increase in o...

Modern preclinical and clinical studies show that the use of diagnostic methods based on the registration of fluorescent radiation can improve the early diagnosis of cancer and other destructive processes of various organs. Currently, many studies are being conducted aimed at studying the processes of oxidative metabolism using methods that registe...

The process and results of numerical Monte Carlo simulation of optical radiation propagation in laser Doppler flowmetry and fluorescence spectroscopy channels of a wearable diagnostic multimodal device are described in this paper. To achieve the goal, a multilayer skin model with different parameters of blood and melanin content and different dista...

This paper presents clinical results of wireless portable dynamic light scattering sensors that implement laser Doppler flowmetry signal processing. It has been verified that the technology can detect microvascular changes associated with diabetes and ageing in volunteers. Studies were conducted primarily on wrist skin. Wavelet continuous spectrum...

Summary Introduction. Yoga breathing exercises make it possible to change the minute ventilation (MV) within a wide range, reaching states of hyper-and hypoventilation with corresponding shifts in gas exchange. In this work, the interrelations between external respiration, parameters of gas metabolism and the skin microcirculation were evaluated. M...

The present work is focused on the study of changes in microcirculation parameters in patients who have undergone COVID-19 by means of wearable laser Doppler flowmetry (LDF) devices. The microcirculatory system is known to play a key role in the pathogenesis of COVID-19, and its disorders manifest themselves long after the patient has recovered. In...

In this study, we assessed the possibility of using portable laser Doppler flowmeters for determiningthe state of the blood microcirculatory system in upper and lower limbs of diabetic patients during the treat-ment course with intravenous α-lipoic acid infusions. A series of experimental studies were conducted in acohort of ten inpatients with a c...

The rising interest in the development of new wearable electronic devices for mobile healthcare can be exemplified by great opportunities for the development of new methods for broader use of blood perfusion monitoring for the purposes of precision medicine. A fine analysis of capillary blood flow structure and its rhythmic oscillations in the time...

Blood microcirculation plays a key role in maintaining tissue homeostasis and is involved in the pathological process in the early stages of the diabetes, leading to severe and difficult-to-treat complications in the future. Vascular tone regulation is an important feature of microvessels and underpins the performance of numerous functions of micro...

Assessment of biological tissues’ intrinsic fluorescence provides unique perspectives for the development of new methods of optical noninvasive diagnostics for application in many fields of medicine. Fluorescence techniques alone or together with other biophotonics methods have found their application in oncology, surgery, dermatology, etc. This ch...

Objective This work aims to study the functional state of resistive skin microvessels in patients with newly diagnosed arterial hypertension (AH), using portable laser Doppler flowmetry (LDF) devices. Materials and methods The study included 89 men aged 30 to 60 years (44.4 ± 8.5) who had no known medical complaints, subjectively considered themse...

This paper presents the first results of the study of changes in microcirculatory-tissue systems functioning under space flight conditions.

The possibility of using the laser Doppler flowmetry (LDF) method for the diagnosis of masked hypertension is considered. Significant trends in changes in parameters between groups of volunteers were identified. This indicates the possibility of using the LDF method to make decisions about the further follow-up of patients for the presence of a hid...

Advances in the development of ultra-compact semiconductor lasers have opened up new possibilities for the development of wearable devices implementing biophotonic technologies, such as laser Doppler flowmetry (LDF) and fluorescence spectroscopy (FS). This work is aimed to evaluate the correlation between diagnostic parameters simultaneously regist...

Introduction. Yoga breathing exercises improve the ability to significantly reduce the respiratory rate. A decrease of the minute respiration volume results in compensatory reactions of the microcirculatory bed caused by changes in the gas composition. The reaction of the regulatory mechanisms of the microvascular bed can be evaluated by the optica...

Biophotonics technologies allow obtaining clinically important diagnostic information about pathological changes that occur in diabetes mellitus, including impaired tissue perfusion and oxygen saturation, structural and functional changes. A review presents the current state‐of‐the‐art in biophotonics technologies and their transfer to clinical use...

This study is devoted to the trials of wearable diagnostic system that implements the laser Doppler flowmetry technique to analyse the blood microcirculation. We do preliminary test with involvement of limited group of healthy volunteers of different age and in patients with type 2 diabetes. During the series of measurements, the microcirculation p...

The problem of diabetes mellitus (DM) has attracted scientists of different specialities, as its prevalence is increasing worldwide and assumes the character of a pandemic. The use of optical non-invasive diagnostic methods is a promising direction for the early determination of the presence and severity of diabetic complications. This work is aime...

Early diagnostics of microcirculation complications is an important area for biomedical photonics application. The blood perfusion measurements are capable of identification of particular markers for diagnostics of many pathological conditions of blood microcirculation in the skin. In this work, we apply the laser Doppler Flowmetry method with the...

This paper provides a brief review of approaches for modeling the interaction of polarized light with turbid media. This paper presents several implementations of Monte Carlo programs that track the polarization state of scattered light. Several classes of models based on the Stokes-Muller formalism and the Jones formalism are considered. Their adv...

In this work, we applied a wearable distributed laser measuring system for the study of regional changes in blood microcirculation in healthy volunteers of different age and diabetic patients. It was found that age-related changes in blood microcirculation can be characterized by increased perfusion level in the fingers and decreased amplitudes of...

Laser Doppler flowmetry (LDF) was used for detection of age-related changes in the blood microcirculation. TheLDF signal was simultaneously recorded from the 3rd fingers’ pads of both hands. Amplitudes of the blood flowoscillations and wavelet coherence of the signals were used for the data analysis. A statistical difference in thesynchronisation o...

Due to simplicity and ease of use, the laser Doppler flowmetry (LDF) has found applications in various areas of research related to non-invasive diagnosis of blood microcirculation. In fact, LDF still has a number of unsolved problems that limit its day-to-day use in clinical practice. Current report discusses the new approach for the LDF signal pr...

The problem of diabetes (DM) has attracted scientists of different specialties, as its prevalence is increasing worldwide and assumes the character of a pandemic. Recent studies have noted that timely diagnosis and treatment, including increasing patient control, reduces complications of DM and makes it possible to reverse their development if dete...

A novel non-invasive, wearable VCSEL-based system for multipoint in − vivo measurements of blood perfusion was introduced. The system operates on the basis of the laser Doppler flowmetry (LDF) method and allows for microcirculation studies. The sensors developed were used to analyse the skin blood flow synchronization in homologous regions of the c...

The possibilities and prospects of optical non-invasive diagnostic technologies for detecting and assessing the severity of microcirculatory and metabolic disorders in rheumatic diseases and diabetes mellitus were assessed. Experimental studies were conducted with the participation of 137 conditionally healthy volunteers and 147 patients with menti...

The purpose of this work is to diagnose microcirculatory disorders in the tissues of psoriatic plaques in patients with psoriasis. Material and methods. Using the method of laser Doppler flowmetry (LDF), local hemodynamic parameters were studied in the place of psoriatic lesion of tissue and in uninvolved skin. Results. In the psoriatic plaque, the...

Local hemodynamic parameters were studied by means of laser Doppler flowmetry in 15 patients with psoriasis in the stationary stage, who have plaques on the inner surface of the forearm. LDF signals recorded at the site of psoriatic lesions of the tissue as well as in the intact tissue at a distance of 1-2 cm from the affected area were analysed. L...

Laser Doppler owmetry (LDF) is widely used to study blood microcirculation in the skin. However, during tradition signal processing based on the integral estimations of the power spectrum of detector photocurrent, the signi�cant part of the information about the skin blood ow is lost. In this study, we propose to analyse the distribution of the b...

To minimisethe influence of blood content on the fluorescence measurements in vivo, a fibre optical probe combining fluorescence and diffuse reflectance measurements was developed. For the inverse solution of the blood content recovery,a set of neural networkstrained by the Monte Carlo generated learning set was used.An approach of fluorescence mea...

The possibility of a complex approach for studying changes in the system of blood microcirculation and metabolic processes in the biotissue of lower extremities using optical noninvasive methods of laser Doppler flowmetry (LDF), fluorescence spectroscopy, and diffuse reflectance spectroscopy in combination with different modes of heating tests has...

The paper shows the possibility of using the laser Doppler flowmetry method for the assessment of system of blood microcirculation in the lower limbs of patients with diabetes mellitus. A series of experimental studies involving 76 patients with diabetes mellitus and 46 healthy volunteers was carried out. The state of peripheral blood flow was asse...

The paper shows the possibility of assessing the functional state of microcirculatory-tissue systems of patients with diabetes mellitus by laser Doppler flowmetry (LDF), diffuse reflectance spectroscopy (DRS) and fluorescence spectroscopy (FS) methods. A review of the existing non-invasive optical technologies used to assess the state of microcircu...

Timely diagnostics of microcirculatory system abnormalities which are the most severe diabetic complications, is a significant problem of modern health care. Functional abnormalities manifest themselves earlier than the structural one and their assessment is the focus of present-day studies. In this study, the Laser Doppler flowmetry, a noninvasive...

Laser Doppler flowmetry (LDF) is widely used for diagnosing blood microcirculation diseases. It is well known that the Doppler shift of laser radiation scattered by moving red blood cells (RBC) can be assessed through analyzing photocurrent produced by a photodetector. LDF signal contains information about regulating blood flow rhythms: myogenic, c...