Elena ZharkikhOrel State University · Research and Development Center of Biomedical Photonics
Elena Zharkikh
Master of Science
About
42
Publications
4,579
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209
Citations
Citations since 2017
Introduction
Entered the Orel State University to the department «Instrumentation, metrology and certification» in 2013.
Since October 2014 is a member of the research and development center of biomedical photonics.
Topic of research:
- methodological provision and instrumentation of fluorescence spectroscopy of biological tissue in vivo;
- investigation of transient processes of changes of blood microcirculation during different functional tests.
Additional affiliations
October 2017 - present
Education
September 2017 - July 2019
September 2013 - July 2017
Publications
Publications (42)
Timely diagnostics of microcirculatory system abnormalities, which are the most severe diabetic complications, is one of the major problems facing modern health care. Functional abnormalities manifest themselves earlier than the structural ones, and therefore their assessment is the issue of primary importance. In this study Laser Doppler flowmetry...
According to the International Diabetes Federation, the challenge of early stage diagnosis and treatment effectiveness monitoring in diabetes is currently one of the highest priorities in modern healthcare. The potential of combined measurements of skin fluorescence and blood perfusion by the laser Doppler flowmetry method in diagnostics of low lim...
In this study, we demonstrate what kind of relative alterations can be expected in average perfusion and blood flow oscillations during postural changes being measured in the skin of limbs and on the brow of the forehead by wearable laser Doppler flowmetry (LDF) sensors. The aims of the study were to evaluate the dynamics of cutaneous blood perfusi...
The prevalence of diabetes complications is a significant public health problem with a considerable economic cost. Thus, the timely diagnosis of complications and prevention of their development will contribute to increasing the length and quality of patient life, and reducing the economic costs of their treatment. This article aims to review the c...
The present work is focused on the study of changes in microcirculation parameters in patients who have undergone COVID-19 by means of wearable laser Doppler flowmetry (LDF) devices. The microcirculatory system is known to play a key role in the pathogenesis of COVID-19, and its disorders manifest themselves long after the patient has recovered. In...
In this study, we assessed the possibility of using portable laser Doppler flowmeters for determiningthe state of the blood microcirculatory system in upper and lower limbs of diabetic patients during the treat-ment course with intravenous α-lipoic acid infusions. A series of experimental studies were conducted in acohort of ten inpatients with a c...
The rising interest in the development of new wearable electronic devices for mobile healthcare can be exemplified by great opportunities for the development of new methods for broader use of blood perfusion monitoring for the purposes of precision medicine. A fine analysis of capillary blood flow structure and its rhythmic oscillations in the time...
Blood microcirculation plays a key role in maintaining tissue homeostasis and is involved in the pathological process in the early stages of the diabetes, leading to severe and difficult-to-treat complications in the future. Vascular tone regulation is an important feature of microvessels and underpins the performance of numerous functions of micro...
Assessment of biological tissues’ intrinsic fluorescence provides unique perspectives for the development of new methods of optical noninvasive diagnostics for application in many fields of medicine. Fluorescence techniques alone or together with other biophotonics methods have found their application in oncology, surgery, dermatology, etc. This ch...
Objective
This work aims to study the functional state of resistive skin microvessels in patients with newly diagnosed arterial hypertension (AH), using portable laser Doppler flowmetry (LDF) devices.
Materials and methods
The study included 89 men aged 30 to 60 years (44.4 ± 8.5) who had no known medical complaints, subjectively considered themse...
This paper presents the first results of the study of changes in microcirculatory-tissue systems functioning under space flight conditions.
The possibility of using the laser Doppler flowmetry (LDF) method for the diagnosis of masked hypertension is considered. Significant trends in changes in parameters between groups of volunteers were identified. This indicates the possibility of using the LDF method to make decisions about the further follow-up of patients for the presence of a hid...
Advances in the development of ultra-compact semiconductor lasers have opened up new possibilities for the development of wearable devices implementing biophotonic technologies, such as laser Doppler flowmetry (LDF) and fluorescence spectroscopy (FS). This work is aimed to evaluate the correlation between diagnostic parameters simultaneously regist...
Introduction. Yoga breathing exercises improve the ability to significantly reduce the respiratory rate. A decrease of the minute respiration volume results in compensatory reactions of the microcirculatory bed caused by changes in the gas composition. The reaction of the regulatory mechanisms of the microvascular bed can be evaluated by the optica...
Biophotonics technologies allow obtaining clinically important diagnostic information about pathological changes that occur in diabetes mellitus, including impaired tissue perfusion and oxygen saturation, structural and functional changes. A review presents the current state‐of‐the‐art in biophotonics technologies and their transfer to clinical use...
This study is devoted to the trials of wearable diagnostic system that implements the laser Doppler flowmetry technique to analyse the blood microcirculation. We do preliminary test with involvement of limited group of healthy volunteers of different age and in patients with type 2 diabetes. During the series of measurements, the microcirculation p...
The problem of diabetes mellitus (DM) has attracted scientists of different specialities, as its prevalence is increasing worldwide and assumes the character of a pandemic. The use of optical non-invasive diagnostic methods is a promising direction for the early determination of the presence and severity of diabetic complications.
This work is aime...
Early diagnostics of microcirculation complications is an important area for biomedical photonics application. The blood perfusion measurements are capable of identification of particular markers for diagnostics of many pathological conditions of blood microcirculation in the skin. In this work, we apply the laser Doppler Flowmetry
method with the...
This paper provides a brief review of approaches for modeling the interaction of polarized light with turbid media. This paper presents several implementations of Monte Carlo programs that track the polarization state of scattered light. Several classes of models based on the Stokes-Muller formalism and the Jones formalism are considered. Their adv...
In this work, we applied a wearable distributed laser measuring system for the study of regional changes in blood microcirculation in healthy volunteers of different age and diabetic patients. It was found that age-related changes in blood microcirculation can be characterized by increased perfusion level in the fingers and decreased amplitudes of...
Laser Doppler flowmetry (LDF) was used for detection of age-related changes in the blood microcirculation. TheLDF signal was simultaneously recorded from the 3rd fingers’ pads of both hands. Amplitudes of the blood flowoscillations and wavelet coherence of the signals were used for the data analysis. A statistical difference in thesynchronisation o...
Due to simplicity and ease of use, the laser Doppler flowmetry (LDF) has found applications in various areas of research related to non-invasive diagnosis of blood microcirculation. In fact, LDF still has a number of unsolved problems that limit its day-to-day use in clinical practice. Current report discusses the new approach for the LDF signal pr...
The problem of diabetes (DM) has attracted scientists of different specialties, as its prevalence is increasing worldwide and assumes the character of a pandemic. Recent studies have noted that timely diagnosis and treatment, including increasing patient control, reduces complications of DM and makes it possible to reverse their development if dete...
A novel non-invasive, wearable VCSEL-based system for multipoint in − vivo measurements of blood perfusion was introduced. The system operates on the basis of the laser Doppler flowmetry (LDF) method and allows for microcirculation studies. The sensors developed were used to analyse the skin blood flow synchronization in homologous regions of the c...
The possibilities and prospects of optical non-invasive diagnostic technologies for detecting and assessing the severity of microcirculatory and metabolic disorders in rheumatic diseases and diabetes mellitus were assessed. Experimental studies were conducted with the participation of 137 conditionally healthy volunteers and 147 patients with menti...
The purpose of this work is to diagnose microcirculatory disorders in the tissues of psoriatic plaques in patients with psoriasis. Material and methods. Using the method of laser Doppler flowmetry (LDF), local hemodynamic parameters were studied in the place of psoriatic lesion of tissue and in uninvolved skin. Results. In the psoriatic plaque, the...
Local hemodynamic parameters were studied by means of laser Doppler flowmetry in 15 patients with psoriasis in the
stationary stage, who have plaques on the inner surface of the forearm.
LDF signals recorded at the site of psoriatic lesions of the tissue as well as in the intact tissue at a distance of 1-2 cm
from the affected area were analysed. L...
Laser Doppler
owmetry (LDF) is widely used to study blood microcirculation in the skin. However, during
tradition signal processing based on the integral estimations of the power spectrum of detector photocurrent,
the signi�cant part of the information about the skin blood
ow is lost. In this study, we propose to analyse the
distribution of the b...
To minimisethe influence of blood content on the fluorescence measurements in vivo, a fibre optical probe combining fluorescence and diffuse reflectance measurements was developed. For the inverse solution of the blood content recovery,a set of neural networkstrained by the Monte Carlo generated learning set was used.An approach of fluorescence mea...
The possibility of a complex approach for studying changes in the system of blood microcirculation and metabolic processes in the biotissue of lower extremities using optical noninvasive methods of laser Doppler flowmetry (LDF), fluorescence spectroscopy, and diffuse reflectance spectroscopy in combination with different modes of heating tests has...
The paper shows the possibility of using the laser Doppler flowmetry method for the assessment of system of blood microcirculation in the lower limbs of patients with diabetes mellitus. A series of experimental studies involving 76 patients with diabetes mellitus and 46 healthy volunteers was carried out. The state of peripheral blood flow was asse...
The paper shows the possibility of assessing the functional state of microcirculatory-tissue systems of patients with diabetes mellitus by laser Doppler flowmetry (LDF), diffuse reflectance spectroscopy (DRS) and fluorescence spectroscopy (FS) methods. A review of the existing non-invasive optical technologies used to assess the state of microcircu...
Laser Doppler flowmetry (LDF) is widely used for diagnosing blood microcirculation diseases. It is well known that the Doppler shift of laser radiation scattered by moving red blood cells (RBC) can be assessed through analyzing photocurrent produced by a photodetector. LDF signal contains information about regulating blood flow rhythms: myogenic, c...
Projects
Project (1)
The goal of the project is the development of the new physical and technical basis of non-invasive methods (laser Doppler flowmetry (LDF), contact thermometry (CT) and optical tissue oxymetry (OTO)) to study hemodynamics microcirculation system.
We plan to combine LDF, CT and OTO measurements on the common platform and to develop the program which allows to measure simultaneously these parameters and to provide post processing which includes statistical and spectral analysis. Using this facility we plan to carry out experiments in the control group and in the group of rheumatic patients using provoking tests.
Under the project we plan to complete the experimental device allowed to measure the described above parameters and create programs for processing of recording experimental data with spectral analysis, statistical analysis and estimating the frequency correlation and phase coherence.
An important part of the work is to determine the physical parameters of the system affecting the characteristics of oscillations and to study the possibility to develop the method for diagnostics disturbance in the microcirculation system from experimental data. As the result it is planned to get criteria for increasing the sensitivity of LDF, OTO and CT. in the study of functional condition and revealing propensity to angiospasm, oxygenation disorders of the fingers of patients with rheumatologic syndromes. A promising area for wider introducing of LDF, OTO and CT methods is associated with variety of functional tests.
