About
3
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Introduction
My research focuses on cell physiology of lactic acid bacteria (LAB) . I study pyruvate metabolism of LAB. More specifically I work on redirecting the metabolic flux from lactate to alcohols. I am also interested in the construction of tools to better understand metabolism, such as genome scale metabolic networks. My overall goal is to determine the suitability of Lactobacillus casei as potential biocatalyst for the production of higher alcohols.
Current institution
Publications
Publications (3)
Microbial fermentation of sugars from plant biomass to alcohols represents an alternative to petroleum-based fuels. The optimal biocatalyst for such fermentations needs to overcome hurdles such as high concentrations of alcohols and toxic compounds. Lactic acid bacteria, especially lactobacilli, have high innate alcohol tolerance and are remarkably...
Lactobacillus casei strains are widely used in industry and the utility of this organism in these industrial applications is strain dependent. Hence, tools capable of predicting strain specific phenotypes would have utility in the selection of strains for specific industrial processes. Genome-scale metabolic models can be utilized to better underst...
A Cheddar cheese model system, Cheddar cheese extract, was used to examine how different levels of known microbial hurdles (NaCl, pH, and lactic acid) in Cheddar cheese contribute to inhibition of bacterial pathogens. This knowledge is critical to evaluate the safety of Cheddar varieties with altered compositions. The range of levels used covered t...
Questions
Question (1)
I am trying to use ddPCR for strain identification/enumeration. I designed primers and probes on a strain specific region and I get positive calls. However, when I tried a pgi gene or general sequence (to get total count) I am not getting any calls. Any ideas? I am testing the primers on single strain culture, so in theory I should be getting the same calls with the general and specific primers.
