Elena Vladimirovna PotapovaOrel State University · Scientific-educational center «Biomedical engineering»
Elena Vladimirovna Potapova
PhD
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69
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364
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Citations since 2017
Publications
Publications (69)
Previous studies have shown that intraoperative optical diagnostics can be used to solve fundamental and applied problems in gynecology. Our article presents results from experimental in vivo intraoperative measurements of endogenous fluorescence and blood perfusion in various sections of the uterus, myometrium, endometrium, myomas, and pseudocapsu...
Modern photonics methods are considered in relation to solving a number of diagnostic problems in practical medicine. The basic principles of the application of optical noninvasive diagnostic methods are described and examples are given: laser Doppler flowmetry, digital diaphanoscopy, Raman spectroscopy, spectrophotometry, time-resolved fluorescenc...
In this work, we demonstrate the results of measuring the optical properties of rat liver tissue slices after the liver itself underwent the perfusion procedure with an isotonic solution. The approach is suggested as a means to attenuate the influence of blood absorption on recorded characteristics and demonstrates its effectiveness in changing the...
Blood microcirculation plays a key role in maintaining tissue homeostasis and is involved in the pathological process in the early stages of the diabetes, leading to severe and difficult-to-treat complications in the future. Vascular tone regulation is an important feature of microvessels and underpins the performance of numerous functions of micro...
Singlet oxygen (1O2) is an electronically excited state of triplet oxygen which is less stable than molecular oxygen in the electronic ground state and produced by photochemical, thermal, chemical, or enzymatic activation of O2. Although the role of singlet oxygen in biology and medicine was intensively studied with photosensitisers, using of these...
At the moment, percutaneous needle biopsy (PNB) remains the gold standard for diagnosing liver cancer. However, the relatively high probability of false-negative results can still be an issue with the method. The introduction of real-time feedback for the precise navigation of the biopsy tool is an up-and-coming technology to immensely reduce the m...
This article presents a liquid phantom technology that simulates the fluorescent properties of protoporphyrin IX (PPIX). A technology was developed for the isolation of PPIX from dark egg shells with a final concentration close to the real values in human tissues. A comparative analysis of the transmittance coefficient of the manufactured phantom a...
The aim of this work is to develop practical tools to recognize the average flow rate of physiological fluids in capillaries. This tool is represented by classification models in an artificial neural networks form. The flow rate data were obtained experimentally. Intralipid was used as the test liquid. Laser speckle contrast imaging was used to obt...
In this work, we propose the results of pilot studies on using the modulated CMOS camera for the imaging of autofluorescence in murine hepatocellular carcinoma cells with excitation in UV light. Here, we assess the capabilities of the imaging system to detect changes in the NAD(P)H fractions produced and utilised in the glycolysis, pentose phosphat...
Neurodegenerative disorders are currently incurable devastating diseases which are characterized by the slow and progressive loss of neurons in specific brain regions. Progress in the investigation of the mechanisms of these disorders helped to identify a number of genes associated with familial forms of these diseases and a number of toxins and ri...
This work presents results of in vivo and in situ measurements of hepatocellular carcinoma by a developed optical biopsy system. Here, we describe the technical details of the implementation of fluorescence lifetime and diffuse reflectance measurements by the system, equipped with an original needle optical probe, compatible with the 17.5G biopsy n...
The article presents the results of studies of the melanin effect on recorded signals in laser Doppler flowmetry (LDF) and tissue reflectance oximetry (TO). By investigating the properties of recorded microvascular blood flow and skin oxygenation signals, we have gained new knowledge about race-related differences in the formation of these signals....
The article describes a multiparametric approach to the assessment of cutaneous microcirculation in dermatological patients (using the example of psoriasis patients). The multiparametric diagnostic approach consisted of three stages of successive measurements performed according to the methodology of laser speckle contrast imaging (LSCI), laser Dop...
Due to the widespread introduction of laparoscopic studies into modern surgical practice, the task of objectifying the obtained data and expanding the opportunities for obtaining additional diagnostic information is urgent. In this work, we develop the first-of-its-kind multimodal laparoscopic system integrating hyperspectral, fluorescence, and las...
In this paper we demonstrate results of combined measurements by diffuse reflectance and fluorescence lifetime measuring techniques for real time liver cancer differentiation using a needle optical probe.
The laser speckle contrast imaging allows the determination of the flow motion in a sequence of images. The aim of this study is to combine the speckle contrast imaging and machine learning methods to recognition of physiological fluids flow rate. Data on the flow of intralipid with average flow rate of 0-2 mm/s in a glass capillary were obtained u...
LSCI technique provides experimental data which can be considered in the context of spatial blood flow coherency. Analysis of vascular tone oscillations gives additional information to ensure a better understanding of the mechanisms affecting microvascular physiology. The oscillations with different frequencies are due to different physiological me...
Based on the combined use of polymerisable polyacrylamide, collagen and an aqueous solution of flavin adenine dinucleotide (FAD), we have developed a new technique for fabrication of composite phantoms mimicking fluorescence properties of human skin. A comparative analysis of the absorption and scattering coefficients, refractive indices, as well a...
A flexible fibre optical probe is implemented to record the parameters of the endogenous fluorescence during minimally invasive interventions in patients with cancers of hepatoduodenal area. Using machine learning techniques, the obtained spectra are classified to indicate cancerous or healthy tissue. For this, a set of different binary classifiers...
Abdominal cancer is a widely prevalent group of tumours with a high level of mortality if diagnosed at a late stage. Although the cancer death rates have in general declined over the past few decades, the mortality from tumours in the hepatoduodenal area has significantly increased in recent years. The broader use of minimal access surgery (MAS) fo...
The aspects of using optical instruments for minimally invasive interventions in abdominal surgery are considered. The results of clinical and preclinical trials of the application of the multimodal optical approaches in endoscopic instruments for the treatment and diagnosis of abdominal cancer are reviewed and discussed. A significant increase in...
This paper describes the experimental monitoring of pancreatic oxidative metabolism in laboratory mice that combines the methods of fluorescence and diffuse reflectance spectroscopy and laser speckle contrast imaging with a pancreatic ischemia model. The combined measurements show a close dependence of tissue metabolism on blood and oxygen supply....
Liver cancer remains one of the most widespread cancer types worldwide. The time spent on the diagnosis is one of crucial factors ensuring the effectiveness of treatment. As percutaneous needle biopsy remains the gold standard for liver diagnosis, it is necessary to develop new methods for improving this procedure. One of the most promising directi...
This paper presents the results of the experiments which were performed using the optical biopsy system specially developed for in vivo tissue classification during the percutaneous needle biopsy (PNB) of the liver. The proposed system includes an optical probe of small diameter acceptable for use in the PNB of the liver. The results of the feasibi...
A device for optical biopsy with a fluorescence spectroscopy channel and a fine-needle optical probe for use in fine-needle aspiration biopsy of liver tumors is described. To test the developed device, experimental measurements of the fluorescence of internal organs of a laboratory rat were carried out in vivo while exposing the tissue surface to m...
Aggregation of the misfolded proteins β-amyloid, tau, huntingtin, and α-synuclein is one of the most important steps in the pathology underlying a wide spectrum of neurodegenerative disorders, including the two most common ones—Alzheimer's and Parkinson's disease. Activity and toxicity of these proteins depends on the stage and form of aggregates....
The article describes the development of the optical phantom mimicking flavin adenine dinucleotide (FAD) and skin collagen fluorescence. The results of experimental studies using fluorescence imaging and fluorescence spectroscopy methods are demonstrated. The method of optical phantom production for quick calibration of fluorescence imaging devices...
To determine optimal lighting conditions for contrast imaging of surgical objects, optical characteristics of biological tissues and spectral characteristics of smart light "LED light" source based on RGBW LED are compared. The spectral characteristics of tissues and organs have been investigated. Optimal lighting conditions for contrast imaging of...
The paper describes the results of experimental studies using custom developed optical biopsy system for diagnostics in vivo during the procedure of fine needle aspiration biopsy. Experimental studies were conducted in laboratory mice with inoculated hepatocellular carcinoma. The measurements were carried out using fluorescence spectroscopy and dif...
The possibility of using a multi-modal approach in the clinical practice of a dermatologist for psoriasis, which includes 3 optical non-invasive technologies: laser Doppler flowmetry, video capillaroscopy, and laser speckle-contrast imaging, is demonstrated. The dynamics of changes in the main microhemodynamic parameters of the microcirculatory bed...
Currently, optical biopsy is a promising area of diagnosing the state of tissues in real time during the surgical treatment of oncological diseases. The important part of this direction is the development of fluorescence imaging systems and ensuring the accuracy of the calibration of optical measurements. The article describes the development of fl...
Laser speckle contrast imaging of the microcirculatory bed of the pancreas is performed, which allows its condition to be assessed and thereby is an additional valuable tool for making a diagnostic decision and dynamically monitoring the effectiveness of the treatment for pathology of the abdominal organs. Laparoscopic operations on the pancreas ar...
During surgery, it is important for the surgeon to determine the optimal lighting conditions for contrast imaging of the operated objects. The paper analyzes various parameters of a controlled colour-dynamic surgical lamp based on a powerful led when contrasting individual organs and tissues of model animals, as well as optical parameters of the or...
Objective:
Spectral analysis of laser Doppler flowmetry (LDF) signals has been widely used in studies of physiological vascular function regulation. An alternative to LDF is the laser speckle contrast imaging method (LSCI), which is based on the same physical principle. In contrast to LDF, LSCI provides non-scanning full-field imaging of a relativ...
Presently, in the modern laser Doppler flowmetry (LDF) the distribution of blood perfusion and its changes along the Doppler shift frequencies are simply ignored and/or not properly addressed. Utilizing the registered power spectrum of photocurrent, we introduce an LDF signal processing approach suitable for expanding of diagnostic capabilities of...
The paper describes the methodology and technical implementation of a multimodal approach for optical diagnostics in hepatopancreatobiliary organs focal and diffuse neoplasms. Fine needle aspiration biopsy technique and following cytological examination show its effectiveness and safety but its performing takes several days. However, the problem of...
The variation of blood flow characteristics caused by the probe pressure during noninvasive studies is of particular interest within the context of fundamental and applied research. It has been shown previously that the weak local pressure induces vasodilation, whereas the increased pressure is able to stop the blood flow in the compressed area, as...
The problem of diabetes (DM) has attracted scientists of different specialties, as its prevalence is increasing worldwide and assumes the character of a pandemic. Recent studies have noted that timely diagnosis and treatment, including increasing patient control, reduces complications of DM and makes it possible to reverse their development if dete...
Fine needle aspiration biopsy technique and following histological examination show its effectiveness and safety but its performing takes several time. However, the problem of real-time analysis of pathological changes in tissues remains relevant. We demonstrate optical fine-needle biopsy method, combining a fine needle (17.5G) and a fiber-optic pr...
The paper presents the results of experimental measurements of endogenous fluorescence and blood perfusion in patients with pathology of the organs of hepatopancreatoduodenal area in vivo. A custom setup combining channels for fluorescence spectroscopy (excitation wavelengths of 365 nm and 450 nm) and laser Doppler flowmetry (1064 nm) with fibre op...
The variation of blood flow characteristics caused by the probe pressure during noninvasive studies is of particular interest within the context of fundamental and applied research. It has been shown previously that the weak local pressure induces vasodilation, whereas the increased pressure is able to stop the blood flow in the compressed area, as...
The recent development of the instrumental methods for two-and three-dimensional imaging allows one to upgrade minimally invasive surgical methods for the abdominal organs to a new level. This type of surgical interventions makes possible to achieve recovery with minimal trauma, reducing the incidence of complications, effectively maintaining and r...
Determination of optimal lighting conditions for
contrast imaging of operated objects is important for the
surgeon. In this paper, we consider various parameters of a
controlled color-dynamic surgical light based on a powerful
LED when contrasting individual organs and tissues of model
animals. The obtained results will allow to develop a library o...
The possibilities and prospects of optical non-invasive diagnostic technologies for detecting and assessing the severity of microcirculatory and metabolic disorders in rheumatic diseases and diabetes mellitus were assessed. Experimental studies were conducted with the participation of 137 conditionally healthy volunteers and 147 patients with menti...
The work is devoted to review the various methods and approaches currently used within the framework of the concept of optical «biopsy» in the study of blood perfusion and metabolism of human organs tissues. The purpose of the review was to study the features and areas of application of these methods and to assess further application possibilities...
The purpose of this work is to diagnose microcirculatory disorders in the tissues of psoriatic plaques in patients with psoriasis. Material and methods. Using the method of laser Doppler flowmetry (LDF), local hemodynamic parameters were studied in the place of psoriatic lesion of tissue and in uninvolved skin. Results. In the psoriatic plaque, the...
Timely diagnostics of microcirculatory system abnormalities, which are the most severe diabetic complications, is one of the major problems facing modern health care. Functional abnormalities manifest themselves earlier than the structural ones, and therefore their assessment is the issue of primary importance. In this study Laser Doppler flowmetry...
At present, minimally invasive interventions become more widespread for treating hepatopancreatoduodenal area pathologies. However, new methods and approaches are necessary for obtaining more diagnostic information in real time. Several methods within the framework of “optical biopsy” concept are considered. The features and areas of application of...
Local hemodynamic parameters were studied by means of laser Doppler flowmetry in 15 patients with psoriasis in the
stationary stage, who have plaques on the inner surface of the forearm.
LDF signals recorded at the site of psoriatic lesions of the tissue as well as in the intact tissue at a distance of 1-2 cm
from the affected area were analysed. L...
The possibility of a complex approach for studying changes in the system of blood microcirculation and metabolic processes in the biotissue of lower extremities using optical noninvasive methods of laser Doppler flowmetry (LDF), fluorescence spectroscopy, and diffuse reflectance spectroscopy in combination with different modes of heating tests has...
The paper shows the possibility of using the laser Doppler flowmetry method for the assessment of system of blood microcirculation in the lower limbs of patients with diabetes mellitus. A series of experimental studies involving 76 patients with diabetes mellitus and 46 healthy volunteers was carried out. The state of peripheral blood flow was asse...
The paper shows the possibility of assessing the functional state of microcirculatory-tissue systems of patients with diabetes mellitus by laser Doppler flowmetry (LDF), diffuse reflectance spectroscopy (DRS) and fluorescence spectroscopy (FS) methods. A review of the existing non-invasive optical technologies used to assess the state of microcircu...
According to the International Diabetes Federation, the challenge of early stage diagnosis and treatment effectiveness monitoring in diabetes is currently one of the highest priorities in modern healthcare. The potential of combined measurements of skin fluorescence and blood perfusion by the laser Doppler flowmetry method in diagnostics of low lim...
Immunoinflammatory reactions affecting the state of the microvasculature play the key role in the genesis of rheumatic diseases. Therefore, it is important to develop new methods for the early detection of microcirculatory disorders. The purpose of this study was to assess the possibilities of diffuse reflectance spectroscopy used to identify micro...
Projects
Projects (3)
Light-based technologies present unique opportunities for the diagnosis of various pathological disorders of biological tissues. With the advancement of modern science, they allow for the non-invasive identification of diseases in the early stages. The range of applications of optical technologies in clinical practice is quite wide, since the optical properties of biological tissues are subject to significant changes in the development of the disease.
This Special Issue will highlight advantages and unique aspects of the use of optical diagnostic methods in identifying and evaluating various diseases and pathological conditions. Articles may focus on the application of technology, disease, or other aspects of the use of spectroscopy and imaging in clinical practice. Articles should reflect novel innovative research and
emerging ideas.
Clinical and experimental substantiation of multiparametric optical biopsy of hepatopancreatoduodenal zone organs for minimally invasive surgical operations
The goal of the project is the development of the new physical and technical basis of non-invasive methods (laser Doppler flowmetry (LDF), contact thermometry (CT) and optical tissue oxymetry (OTO)) to study hemodynamics microcirculation system.
We plan to combine LDF, CT and OTO measurements on the common platform and to develop the program which allows to measure simultaneously these parameters and to provide post processing which includes statistical and spectral analysis. Using this facility we plan to carry out experiments in the control group and in the group of rheumatic patients using provoking tests.
Under the project we plan to complete the experimental device allowed to measure the described above parameters and create programs for processing of recording experimental data with spectral analysis, statistical analysis and estimating the frequency correlation and phase coherence.
An important part of the work is to determine the physical parameters of the system affecting the characteristics of oscillations and to study the possibility to develop the method for diagnostics disturbance in the microcirculation system from experimental data. As the result it is planned to get criteria for increasing the sensitivity of LDF, OTO and CT. in the study of functional condition and revealing propensity to angiospasm, oxygenation disorders of the fingers of patients with rheumatologic syndromes. A promising area for wider introducing of LDF, OTO and CT methods is associated with variety of functional tests.