Elena Lavrova

Elena Lavrova
Sechenov Institute of Evolutionary Physiology and Biochemistry · Laboratory of Comparative Biochemistry of Cellular Functions

PhD

About

88
Publications
883
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272
Citations
Citations since 2016
8 Research Items
65 Citations
201620172018201920202021202202468101214
201620172018201920202021202202468101214
201620172018201920202021202202468101214
201620172018201920202021202202468101214
Introduction
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Publications

Publications (88)
Article
Full-text available
One of the main challenges of modern neurobiology is finding approaches to prevent structural abnormalities in the brain, specifically, in the hippocampus affected by epileptic activity. It is well known that epilepsy leads to an increase in proliferation in the hippocampal neurogenic niche, the subgranular layer of the dentate gyrus. In the recent...
Article
We report here studies of the role of the ERK1/2 signal cascade in regulating the activity of vasopressinergic neurons in the hippocampus in intact Krushinskii–Molodkina (KM) rats and during and after convulsive seizures. No differences were seen in phospho-ERK1/2 kinase or phospho-CREB contents in the supraoptic nucleus (SON) of the hypothalamus b...
Article
Previously we showed that arginine-vasotocin (AVT)-stimulated osmotic water permeability (OWP) of the frog urinary bladder was decreased if the mucosal side of the bladder has been naturally colonized by Gram-negative bacteria, or if bacterial lipopolysaccharide (LPS) was introduced into the lumen of the isolated bladder (J. Exp. Zool., 2013, 319,...
Article
Full-text available
The effect of bacterial lipopolysaccharide (LPS) on eukaryotic cell could be accompanied by a significant metabolic shift that includes accumulation of triacylglycerol (TAG) in lipid droplets (LD), ubiquitous organelles associated with fatty acid storage, energy regulation and demonstrated tight spatial and functional connections with mitochondria....
Article
Recently, we demonstrated that inhibition of ERK1/2 activity by SL-327 treatment blocks seizure behavior in Krushinsky-Molodkina (KM) rats, which was mediated by altering of GABA and glutamate release mechanism in the hippocampus. Basal ganglia representing various subcortical cell groups play a significant role in the regulation of motor activity,...
Article
It is known that in various cell types bacterial lipopolysaccharide (LPS) causes mitochondrial disfunction and promotes accumulation of triglycerides in intracellular lipid droplets. The precise mechanisms which mediate LPS-induced neutral lipid deposition remain poorly understood. In the present work performed on primary cultured epithelial cells...
Article
It was shown previously that colonization of the frog urinary bladder by gram-negative bacteria leads to decreased ability of antidiuretic hormone to reabsorb water from the urinary bladder (Fock et al. J. Exp. Zool., 2013, 319A: 487-494). In the present work performed on epithelial cells isolated from the frog urinary bladder the influence of E. c...
Article
Neutral lipids are deposited in intracellular compartments called lipid droplets, which are known to be critically implicated in regulation of cellular lipid metabolism. These organelles consist of a core of neutral lipids, mainly triacylglycerol (TAG) and cholesteryl esters, surrounded by phospholipid monolayer. Using Nile red lipid staining and [...
Article
In frogs and toads the urinary bladder is very important for the maintenance of water balance due to its ability to store water which can be reabsorbed under the action of arginine-vasotocin (AVT). The usage of isolated bladders as a model for studying the osmotic water permeability (OWP) regulation has a disadvantage which relates to high variabil...
Article
Full-text available
As in mammals, epithelium of the amphibian urinary bladder forms a barrier to pathogen entry and is a first line of defense against penetrating microorganisms. We investigated the effect of E.coli lipopolysaccharide (LPS) on generation of NO, a critically important mediator during infectious processes, by primary cultured frog (Rana temporaria) uri...
Article
The influence of endogenous gram-negative bacteria colonizing the mucosal epithelium of frog Rana temporaria L. urinary bladders (FUB) on arginine-vasotocin AVT-stimulated osmotic water flow in isolated urinary bladders was investigated. 170 animals were examined and only 40% were contaminated with gram-negative bacteria (about 10(3)-10(6) CFU per...
Article
Earlier we have shown that in epithelial cells of the frog urinary bladder under action of bacterial lipopolysaccharides (LPS) there is activated expression of inducible NO-synthase (iNOS) and there is increased the NO production, which can play an important role in providing protective cell reactions from pathogens. The goal of the present work co...
Article
Since Gram-negative bacteria are known to be present in the cavity of urinary bladders in amphibian species, it was interesting to study the effect of bacterial endotoxins on epithelial signaling network which provides the arginine-vasotocin-induced increase of osmotic water permeability (OWP). The effect of LPS E. coli on AVT-induced OWP was studi...
Article
We have shown previously that endogenous NO modulates the effect of arginine-vasotocin on the increase in the osmotic water permeability of the frog urinary bladder epithelium. The aim of the present work was to develop a procedure of cultivation of epithelial cells from the frog urinary bladder as a primary culture in order to study in vitro the c...
Article
The activity of arginase converting arginine into ornithine and urea is of particular interest among many factors regulating NO production in the cells. It is known that by competing with NO-synthase for common substrate (arginine), arginase can affect NO synthesis. In the present work, properties of arginase from the common frog Rana temporaria L....
Article
Full-text available
PGE(2) is a well-known inhibitor of the antidiuretic hormone-induced increase of osmotic water permeability (OWP) in different osmoregulatory epithelia; however, the mechanisms underlying this effect of PGE(2) are not completely understood. Here, we report that, in the frog Rana temporaria urinary bladder, EP(1)-receptor-mediated inhibition of argi...
Article
In experiments on frog Rana temporaria L. urinary bladder, we investigated localization of NO-synthase (NOS) in urinary bladder slices and measured NOS activity in the suspension of mucosal epithelial cells. Intensive NADPH-diaphorase staining which is widely used as an indicator of NOS activity was found in mucosal epithelium. Almost all mucosal e...
Article
(1) The Na, K, Ca, Mg concentration of blood serum have been studied in nearly 60 fish species from various waterbodies (the Neva, Lake Dal'nee, the Volga, Lake Baikal, the White Sea, the Black Sea, the Barents Sea etc.). In fishes (Cyprinidae, Percidae, Siluridae etc.) from fresh water (Lake Dal'nee, the Baikal, the Neva) with low 0.18–0.24 mEq/1...
Article
The present study addressed the question of whether nitric oxide (NO) participates in regulation of osmotic water permeability in the urinary bladder of the frog Rana temporaria L. Experiments were carried out on isolated, paired hemi-bladders filled with amphibian Ringer solution diluted 1:10 with distilled water. Sodium nitroprusside (SNP, 125-25...
Article
The role of atrial natriuretic factor (ANF) in regulation of osmotic water permeability was studied in isolated frog Rana temporaria L. urinary bladder. It was found that ANF (rANF, 1-28) added to the serosal solution at concentrations 5 x 10(-8) M and higher dosedependently stimulated the arginine-vasotocin (AVT)-induced increase of osmotic water...
Article
In frogs' isolated urinary bladders, contribution of cytosolic guanylate cyclase and cGMP-dependent protein kinase to regulation of osmotic permeability was studied. ODQ (25-100 microM), an inhibitor of cytosolic guanylate cyclase induced an increase of vasotocin-activated osmotic permeability but had no effect on the hormone-activated transepithel...
Article
The present study was performed to investigate the role of prostaglandin E2 (PGE2) in the regulation of urea transport in the frog urinary bladder, which is known to occur via a specialized arginine-vasotocin- (AVT-) regulated urea transporter. The bladders isolated from Rana temporaria L. were filled with amphibian Ringer solution containing 370 B...
Article
Principal similarities between molecular pathways providing the enhancement of water and urea reabsorption under the action of argininvasotocin (AVT) in amphibian urinary bladder suggest that prostaglandin E2 (PGE2) could be a negative regulator of urea transport. To analyse this hypothesis, the role of PGE2 in regulation of urea transport was stud...
Article
In active summer and hibernating frogs, effects of arginine-vasotocin (AVT) and prostaglandin E2 (PGE2) on osmotic permeability of isolated urinary bladder have been studied. Despite that the initial osmotic permeability level in the summer frogs was higher than in the winter animals, sensitivity to action of AVT used in the 10-12-10-10 M concentra...
Article
Arginine-vasotocin-induced enhancement of osmotic water flow was inhibited by PGE2, sulprostone, M&B 28767 and 17-phenyl-omega-trinor PGE2. The inhibitory action of these agents except M&B 28767 was more obvious when the osmotic water flow was activated by dibutyryl-cAMP. The findings suggest that, in the frog urinary bladder, the inhibitory effect...
Article
Studying the action of the two antitumour platinum compounds--cisplatin capable of exerting a nephrotoxic action and cycloplatam which has no damaging effect on the kidney, it was found that 3 h after the administration of cycloplatam the content of platinum in the kidney was 2 times lower than in the cfse of cisplatin. Due to different dynamics of...
Article
Studyng the action of the two antitumour platinum compounds - cisplatin capable of exerting a nephrotoxic action and cycloplatam which has no damaging effect on the kidney, it was found that 3 h after the administration of cycloplatam the content of platinum in the kidney was 2 times lower than in the cfse of cisplatin. Due to different dynamics of...
Article
Full-text available
Na, K, Ca and Mg concentrations have been determined in the otoliths and otoconia of the lamprey, skate, guppy and hen. Lamprey otoliths sharply differ from the otoliths and otoconia of other animals with respect to their K and Ca content. In all the animals investigated excluding lamprey, only traces of K were found. Na concentration in the otilit...
Article
1.1. After abrupt transfer of salmon smolts from freshwater (FW) to sea water (SW), fast changes of blood ionic concentrations were observed. During the first 10 min. Na+ concentration quickly rose and K+ concentration dropped by ca 15 and 30%, respectively, and then returned to the control levels; Ca2+ and Mg2+ concentrations increased by 50%.2.2....
Article
Contents of water, sodium, calcium and magnesium in different tissues of the rats stayed in space for 2 weeks in healing period of bone fracture following graded trauma of calf-bones as well as in the rats exposed to a head-down suspension (HDS) have been studied. In microgravity-exposed animals there were no significant changes in fluid-electrolyt...
Article
Frogs acclimated to 4 degrees C were transported to a medium with temperature 20 degrees C, which caused polyuria; recovery of normal diuresis took about 24 h. During this period, hypernatremia was observed together with the increase in natriuresis, the rate of renal excretion of potassium ions with urea remaining constant. Water content of skeleta...
Article
Intra-abdominal injections of platinum, chromium and cadmium salts to Myoxocephalus scorpius produce nephrotoxic effect which includes the disturbances in magnesium secretion. Basic ultrastructural changes in the nephron cells of the fish after the injection of nephrotoxic substances are similar to those in mammals. Cis-platinum induces significant...
Article
In animals in which sodium is a predominant cation of the blood serum or haemolymph (rat, newt Triturus vulgaris, sturgeon Acipenser güldenstädti, herring Clupea pallasi, ascidian Goniocarpa rustica), its content in the body amounts up to a half of the total bulk of electrolytes; in insects with potassium type of the haemolymph, the share of sodium...
Article
After the 7-day space flight onboard the biosatellite Cosmos-1667 the water, Na, K, Ca and Mg content of the liver, kidney, heart, skin and bone of male rats was measured. No significant changes in the weight or water content of the above organs were seen. The exception was a decrease of water contained in the heart and an increase of water contain...
Article
The female rats were flown on Cosmos-1514 for 5 days during gestation days 13 through 18. The rats showed a significant reduction of the Ca concentration in the liver and kidneys, its smaller decrease in the skin, and no changes in bones. The fetus weight decreased, its water content increased, and the content of Na, K, Ca and Mg remained unchanged...
Article
Full-text available
Clawed frog larvae (stages 45-46) that developed for 9 and 8 days beginning with the blastula and tail bud stages, respectively, in the weightless state were investigated. Scanning microscopy of the larval labyrinths did not reveal significant qualitative changes in the receptor and supporting cells of the maculae and in the otolith membrane. Deter...
Article
In the experiments performed on kidneys of 5 species of marine Teleostei, morphological peculiarities in secretion of 8 fluorescent organic acids (uranin, primulin, tripaphlavin, erythrosin etc.) and in 5 organic bases (rhodamin C, auramin etc.) have been studied. At a very low concentration in the incubation medium--about 0.005 mg/ml--the substanc...
Article
1. 1. The osmolality of blood serum of one group of Russian sturgeon fished out in Southern Caspian Sea was the same as their habitat, the other group of sturgeons had a serum osmolality several per cent lower than that of sea-water in this part of the Caspian Sea. 2. 2. Ion concentrations in blood serum in sturgens were similar in fresh water and...
Article
From the 19th day of embryonic period, within 4 days, i. e. up to the beginning of postnatal life, the weight of the heart in rats increases by 3.3 times, that of the liver--by 2.2 times, and kidneys--by 5.7 times, whereas the content of water (calculated per dry weight of the organs) decreases. Changes in the content of sodium, potassium, calcium...
Article
From the 15th to 21st day of pregnancy the mass of foetus increases by 23.5 times in rats; water content decreases from 10.90 +/- 0.36 to 6.63 +/- 0.47 g H2O/g dry substance. During growth of foetus and placenta the amount of sodium, potassium, calcium, magnium, ferrum and cuprum increases in a parallel way in both of them. In the course of embryog...
Article
The concentration index U-P Osm in dehydration was 7.9 in gerbil, 2.7 in water rats and 1.7 in the Brattleboro homozygote rats. The sodium content in the kidney medullar substance of the animals under study correlated with the ability for creation of the high concentration gradient U-P Osm. The sodium excretion for 100 ml of filtrate, in maximal su...
Article
1. After iso-osmotic salt loading (1% NaCl, 1.25% KCl, 0.75% MgCl2 solutions, each load making up 5% body weight) the water voles excreted 66.2% sodium, 84.4% potassium, 18.8% magnesium over a 4 hr period. The big gerbil excreted 20%, 58.9% and 7.1% respectively over the same period. The volume of the water excreted was greater in the case of the w...
Article
Potential difference across the frog skin is increased 1-2 min after addition of 0.063-1.0 mg/ml ethacrynic acid or 0.2-1.0 mg/ml mercusal to outside Ringer solution. Within this time the short-circuit current remains unchanged or increased. Potential difference and short-circuit current are diminished after the addition of ethacrynic acid or mercu...
Article
In the course of evolution of the invertebrate gravity receptor endogenous intracellular mode of the otoliths formation (Coelenterata, Ctenophora) is changed for endogenous extracellular mode (Mollusca, Arthropoda). The intracellular otolith is represented by cristalline concretions whereas extracellular one may consists of many separate crystallic...
Article
1.1. Rodents of the species studied are able to produce a hypoosmotic urine at water diuresis; the urine to plasma osmotic concentration ratio under dehydration in the water vole is 3.8 ± 0.3, in the albino rat—7.0 ± 0.2, and in the big gerbil—12.7 ± 0.9.2.2. Microanatomical data agree with the hypothesis of the countercurrent multiplication.3.3. T...
Article
1.1. The water content, volume of inulin space, Na, K, Mg content and protein synthesis in the adductor of bivalve molluscs Mytilus edulis L. and in the foot muscle of the snails (Gastropoda) Littorina littorea (L.) were investigated at different environmental salinity.2.2. The reaction of mollusc cells to the decreasing of salinity appears to be a...
Article
Administration of water and 0.9% NaCl; 1.25% KCl; 0.75% MgCl2 solutions (5 ml/100 g body weight) into the rat stomach revealed that large amount of water was not detained in the liver but just passed through it. The electrolytes contents did not change significantly in the liver. Administration of KCl and MgCl2 solutions increased concentration of...
Article
To investigate the role of neurohumoral factors in acclimation of mussel muscle to a lowered salinity, studies have been made on the reaction of the intact mussel muscle and that of isolated muscle to change in the salinity from 26% to 10%. The general pattern of swelling of the adductor and the dynamics of its electrolyte composition in intact mol...
Article
During PAH excretion and 18 h after repeated PAH administrations to rats, renal cortical SDH activity was unchanged in comparison with untreated controls. On the other hand, 18 h after repeated administrations of 0.9% or 1.8% NaCl solution, SDH activity was decreased by about 20% in kidney cortex. In outer medulla SDH activity was decreased 18 h af...
Article
Administration of 0.25% -- 2.0% solutions of MgCl2 (5 ml/100 g) into the stomach of unanesthetized rats revealed that it is the 0.75% solution which is most efficiently removed. The kidney removes 3.9% of administered magnesium within 4 hrs, and 18.9%--within 24 hrs. All the administered fluid is absorbed within 3 hrs, but 32% of magnesium salts re...
Article
1.1. The dry solids of eggs and larvae are gradually decreased, the most pronounced mass reduction being observed in larvae. In the process of egg development the water content is significantly increased after fertilization and the transition to larva state.2.2. In the first two days after fertilization a loss of a minor amount of Na+ is observed,...
Article
Concentration of osmotically active substances in the blood serum of the sturgeon A. guldenstaedti and sevruga A. stellatus from the central part of the Caspian Sea is practically equal to that in the sea water. In the blood serum of the sturgeon and the sevruga, concentration of Na is higher, whereas concentration of K and Mg is lower than in the...
Article
1.1. Black sculpine with partly aglomerular kidneys from the Barents Sea and black sculpine with glomerular kidneys from the White Sea as well as scorpion fish and mullet from the Black Sea were injected intramuscularly with Lasix, Edecrin, Brinaldix and Diamox. The kidneys of the different species of these fish showed wide variations in their reac...
Article
Сердепные гликозиды с в 19-OM no-ложении молекулы оказались наиболее зффективными ингигиторами натриевого наоаса клеток кожи лягшки. Налицие ацетильного радикала увелицвало ингибирумуп способнность гликозида; агликон — оуабагенин оказался самым слабым инибитором.