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Elena Gianchecchi

Elena Gianchecchi
VisMederi

Biological Science

About

74
Publications
15,407
Reads
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1,044
Citations
Additional affiliations
October 2012 - September 2013
Ospedale Pediatrico Bambino Gesù
Position
  • PostDoc Position
Education
November 2008 - October 2011
Università degli Studi di Siena
Field of study
  • Biomedicine and Immunological Science

Publications

Publications (74)
Article
Full-text available
Yellow fever (YF) virus still represents a major threat in low resource countries in both South America and Africa despite the presence of an effective vaccine. YF outbreaks are not only due to insufficient vaccine coverage for insufficient vaccine supply, but also to the increase in people without history of vaccination living in endemic areas. Gl...
Article
Full-text available
Since the first detection of a novel Coronavirus (SARS-CoV-2) in December 2019 in Wuhan (China), it has become crucial to assess and quantize the human humoral immune response after SARS-CoV-2 natural infection and/or vaccination. Having well standardized and reliable serological assays able to accurately measure the total IgG antibodies response a...
Article
Full-text available
Siglecs are sialic acid-binding immunoglobulin-like lectins. Most Siglecs function as transmembrane receptors mainly expressed on blood cells in a cell type-specific manner. They recognize and bind sialic acids in specific linkages on glycoproteins and glycolipids. Since Sia is a self-molecule, Siglecs play a role in innate immune responses by dist...
Article
Full-text available
Background: Vaccine effectiveness relies on various serological tests, whose aim is the measurement of antibody titer in serum samples collected during clinical trials before and after vaccination. Among the serological assays required by the regulatory authorities to grant influenza vaccine release there are: Hemagglutination inhibition (HAI), mic...
Article
Full-text available
Autoimmune diseases recognize a multifactorial pathogenesis, although the exact mechanism responsible for their onset remains to be fully elucidated. Over the past few years, the role of natural killer (NK) cells in shaping immune responses has been highlighted even though their involvement is profoundly linked to the subpopulation involved and to...
Article
Introduction: Leishmaniasis represents one of the most dangerous neglected tropical diseases. The parasite used to show a well-defined geographical distribution; however, during the last decade the parasite has spread into new areas. This change in the worldwide distribution of the parasite and in leishmaniasis epidemiology are the result of man’s...
Article
Full-text available
In recent years, the interest in natural compounds exerting immunoregulatory effects has enormously increased. Among these, the polyphenol resveratrol, found in a variety of foods and beverages, including red grapes and red wine, has been demonstrated to exert both in vitro and in vivo biological activities. More specifically, it has antiaging, car...
Article
Full-text available
Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translate...
Article
Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of a large offline gold-standard benchmark of relevant documents that cover a variety of research fields such that newly developed literature search techniques can be compared, improved and translate...
Article
Full-text available
Secretory IgAs (sIgA) constitute the principal isotype of antibodies present in nasal and mucosal secretions. They are secreted by plasma cells adjacent to the mucosal epithelial cells, the site where infection occurs, and are the main humoral mediator of mucosal immunity. Mucosally delivered vaccines, such as live attenuated influenza vaccine (LAI...
Poster
Background. Haemagglutination Inhibition (HAI) assay, MicroNeutralization (MN) assay and Single Radial Haemolysis (SRH) assay are the three main laboratory tests to detect the presence of anti-Influenza antibodies. A correlate of protection is available for SRH and HAI consisting of a halo of haemolysis of 25mm2 and a HAI titer of 40, respectively,...
Article
Full-text available
Autoimmune disorders derive from genetic, stochastic, and environmental factors that all together interact in genetically predisposed individuals. The impact of an imbalanced gut microbiome in the pathogenesis of autoimmunity has been suggested by an increasing amount of experimental evidence, both in animal models and humans. Several physiological...
Article
Influenza vaccines are a fundamental tool for preventing the disease and reducing its consequences, particularly in specific high-risk groups. In order to be licensed, influenza vaccines have to meet strict criteria established by European Medicines Agency. Although the licensure of influenza vaccines started 65 years ago, Hemagglutination Inhibiti...
Article
Full-text available
Influenza vaccines are a fundamental tool for preventing the disease and reducing its consequences, particularly in specific high-risk groups. In order to be licensed, influenza vaccines have to meet strict criteria established by European Medicines Agency. Although the licensure of influenza vaccines started 65 years ago, Hemagglutination Inhibiti...
Article
Full-text available
Regulatory T (Treg) cells represent a subpopulation of suppressor CD4+ T cells critically involved in the establishment of peripheral tolerance through the inhibition of effector T (Teff) cells and the suppression of the immune-mediated tissue destruction toward self-antigens. Treg generation, their suppressive properties and also Treg-Teff cell in...
Article
Full-text available
Type 1 diabetes (T1D) affects millions of people worldwide and is the prevalent form of all pediatric diabetes diagnoses. T1D is recognized to have an autoimmune etiology, since failure in specific self-tolerance mechanisms triggers immune reactions towards self-antigens and causes disease onset. Among all the different immunocytes involved in T1D...
Article
Full-text available
Since the mid-1980s, two lineages of influenza B viruses have been distinguished. These can co-circulate, limiting the protection provided by inactivated trivalent influenza vaccines (TIVs). This has prompted efforts to formulate quadrivalent influenza vaccines (QIVs), to enhance protection against circulating influenza B viruses. This review descr...
Article
Full-text available
The safety of vaccines is a critical factor in maintaining public trust in national vaccination programs. Vaccines are recommended for children, adults and elderly subjects and have to meet higher safety standards, since they are administered to healthy subjects, mainly healthy children. Although vaccines are strictly monitored before authorization...
Article
Full-text available
Type 1 diabetes is an autoimmune disease, in which pancreatic β cells are destroyed by autoreactive T cells in genetically predisposed individuals. Serum beta cell autoantibody specificities have represented the mainstay for classifying diabetes as autoimmune-mediated and for stratifying risk in first-degree relatives. In recent years, approaches w...
Data
FACS gating strategy to analyze different GAD65 AA 114–122 AA 114–122 pentamer reactive subsets after GAD65 peptide plus IL-2 or IL-2 incubation alone. Representative dot plot analysis showing GAD65 AA 114–122 HLA A*02:01 pentamer reactivity on peripheral blood lymphocytes of a T1D patient and a healthy control gated on CD3+CD8bright, CD3+CD8dull a...
Data
Increased percentages of GAD65 AA 114–122 HLA A*02:01 pentamer reactive cells in T1D patients vs healthy controls. Relative frequencies of CD3+CD8bright (A), CD3+CD8dull (B), total CD3+CD8+ (C) and CD3-CD8dull (D) GAD65 AA 114–122 pentamer reactive cells in T1D patients (square dots) vs healthy controls (circle dots) after GAD65 AA 114–122 peptide...
Data
Correlation of GAD65 pentamer reactive cells with metabolic markers. (A) No correlation with total cholesterol levels; (B) No correlation with HDL levels; (C) No correlation with LDL levels; (D) No correlation with triglycerides levels. (TIF)
Data
Sex, age and diabetes-related autoantibodies profile in 20 long-term T1D patients used to define percentages of GAD65 pentamer reactive NK cells. (DOCX)
Article
The pathogenesis of autoimmunity remains to be fully elucidated, although the contribution of genetic and environmental factors is generally recognized. Despite autoimmune conditions are principally due to T and B lymphocytes, NK cells also appear to play a role in the promotion and/or maintenance of altered adaptive immune responses or in peripher...
Article
Introduction: Invasive Meningococcal Disease (IMD) represents a potentially life-threatening condition caused by Neisseria meningitidis. The disease is characterized by a case fatality rate of 5-10% whereas serious clinical sequelae can develop in survivors within 12-24 hours from the first symptoms. However, IMD infection only occurs rarely, in f...
Article
Full-text available
INTRODUCTION: Invasive Meningococcal Disease (IMD) represents a potentially life-threatening condition caused by Neisseria meningitidis. The disease is characterized by a case fatality rate of 5-10% whereas serious clinical sequelae can develop in survivors within 12-24 hours from the first symptoms. However, IMD infection only occurs rarely, in...
Article
Full-text available
Type 1 diabetes and thyroid disease are T cell-dependent autoimmune endocrinopathies. The standard substitutive administration of the deficient hormones does not halt the autoimmune process; therefore, development of immunotherapies aiming to preserve the residual hormonal cells, is of crucial importance. PTPN22 C1858T mutation encoding for the R62...
Data
Cell line authentication results. Genetic characteristics were determined by PCR-single-locus-technology on genomic DNA derived from Jurkat cells used for this study. Twenty-one independent PCR-systems were investigated (Eurofins Medigenomix Forensik GmbH, Ebersberg, Germany). Obtained data were searched and compared with online databases of human...
Data
Lyp protein expression in Jurkat T cells 48 hours after the beginning of O/N transfection with different doses of siRNA s/a in DMPC/2/siRNA lipoplexes. (A) Lyp expression in Jurkat T cells cultured in RPMI or after O/N transfection with DMPC/2, 20 pmols of siRNA (siRNA20) and 20 pmols of siRNA complexed in DMPC/2/siRNA lipoplexes (DMPC/2/siRNA20)....
Data
Lyp protein expression in a healthy volunteer PBMC 72 hours after the beginning of O/N transfection with different doses of siRNA s/a in DMPC/1/siRNA lipoplexes. Figure shows representative WB image with all experimental groups. Cells were transfected with empty liposome, 100 pmols of siRNA alone, DMPC/1/siRNA 60, 80 and 100 pmols or cultured in RP...
Data
Chemical structure of liposomal formulations. (TIF)
Data
Additional WB image. Results of siRNA1 transfection of Jurkat T cells with the commercial transfection system. (TIF)
Data
Analysis of Lyp protein expression in Jurkat T cells 48 and 72 hours after each transfection period with different doses of siRNA s/a complexed in DMPC/1/siRNA lipoplexes. Graphs show the analysis of Lyp protein expression 48 (upper panel) and 72 hours (lower panel) after the three different transfection periods in all control and lipoplexes treate...
Data
Analysis of Lyp protein expression in Jurkat T cells 48 and 72 hours after each transfection period with different doses of siRNA s/a complexed in DMPC/2/siRNA lipoplexes. Lyp protein expression 48 (upper panel) and 72 hours (lower panel) after transfection periods in all control and lipoplexes treated groups. DMPC/2: n = 8; RPMI: n = 7; siRNA100:...
Data
Analysis of Lyp protein expression in PBMC 48 and 72 hours after each transfection period with different doses of siRNA s/a complexed in DMPC/2/siRNA lipoplexes. Lyp protein expression 48 and 72 hours after the O/N transfection period in all control and lipoplexes treated groups. DMPC/2: n = 7; RPMI: n = 7; siRNA100: n = 3; DMPC/2/siRNA60: n = 3; D...
Data
PBMC DMPC/2/siRNA internalization. HD PBMC were administered with the indicated formulation marked with rhodamine and relative controls. After 4 and a half hours of treatment cells were fixed and stained for confocal microscopy. (A) Three stainings were performed on PBMC by using: FITC anti human CD3 (green) antibody to specifically label T cells,...
Data
IL-2 detection ELISA assay of culture supernatants. Histogram shows the increase in IL-2 production from anti-CD3/CD28 beads treated Jurkat cells specifically after DMPC/2/siRNA administration at different doses. * indicates p<0.05. (TIF)
Data
Proliferative assay of DMPC/2/siRNA Jurkat T cells. Values of median fluorescence intensity (MFI) indicative of proliferation obtained from CMFDA-labelled Jurkat T cells after 48 and 72 hours from the beginning of transfection with DMPC/2 lipoplexes respect to untransfected cells. (DOCX)
Data
Lyp protein expression in a healthy donor PBMC 72 hours after the beginning of O/N transfection with different doses of siRNA s/a in DMPC/2/siRNA lipoplexes. (TIF)
Data
IL-2 detection ELISA assay of culture supernatants. Histograms illustrate the unresponsiveness of IL-2 upon 24 (A, C) or 48 (B, D) hours of PMA/IONO (PMA-Ionomycin) stimulation in Jurkat T cells previously transfected with DMPC/2 liposomal formulation. US = control unstimulated cells; H = hours; PMA/IONO = transfected Jurkat T cells following activ...
Data
Design of siRNA duplexes to PTPN22. Table reports the design of duplex siRNAs SNP_C (s and a) against the wild type PTPN22 allele. SNPs were designed by genetists at Sigma Chemical Co. (DOCX)
Article
Full-text available
Type 1 diabetes (T1D) is an autoimmune disorder characterized by the selective destruction of insulin-producing β cells as result of a complex interplay between genetic, stochastic and environmental factors in genetically susceptible individuals. An increasing amount of experimental data from animal models and humans has supported the role played b...
Article
Full-text available
Two different influenza vaccines are generally used in many countries; trivalent live attenuated influenza vaccine (LAIV3) and trivalent inactivated influenza vaccine (IIV3). Studies comparing the antibody response to IIV3 and LAIV3 commonly investigate the seroprotective response by hemagglutination-inhibition (HI) assay. However, there is limited...
Article
APECED is a T-cell mediated disease with increased frequencies of CD8+ effector and reduction of FoxP3+ T regulatory cells. Antibodies against affected organs and neutralizing to cytokines are found in the peripheral blood. The contribution of B cells to multiorgan autoimmunity in Aire-/- mice was reported opening perspectives on the utility of ant...
Article
Full-text available
N. meningitidis infections represent a global health problem that can lead to the development of serious permanent sequelae. Although the use of antibiotics and prevention via vaccination have reduced the incidence of meningococcal disease, our understanding regarding N. meningitidis pathogenesis is still limited, especially of those mechanisms res...
Article
Introduction: Neisseria meningitidis infections represent a serious health problem that can lead to invasive meningococcal disease (IMD), a life-threatening condition associated with significant morbidity and mortality. IMD could however be preventable via vaccination. During the past five decades, vaccines against N. meningitidis capsular groups...
Article
Full-text available
Severe influenza infections are responsible for 3-5 million cases worldwide and 250,000-500,000 deaths per year. Although vaccination is the primary and most effective means of inducing protection against influenza viruses, it also presents limitations. This review outlines the promising steps that have been taken toward the development of a broadl...
Article
Full-text available
Introduction: Vaccines and antiviral drugs are the most widely used methods of preventing or treating Influenza virus infection. The role of sea buckthorn (SBT) bud dry extract as a natural antiviral drug against Influenza was investigated. Methods: Influenza virus was cultured in the MDCK cell line, with or without SBT bud extract, and virus gr...
Article
Full-text available
Neisseria meningitidis is a Gram-negative β-proteobacterium responsible for an endemic worldwide infection. The epidemiology and serogroup distribution can change very quickly. The incidence of meningitis infection varies from very rare to more than 1000 cases per 100,000 of the population yearly. The carriage of N. meningitidis, which represents a...
Article
Full-text available
Type 1 diabetes is an autoimmune disease caused by the destruction of pancreatic beta cells by autoreactive T cells. Among the genetic variants associated with type 1 diabetes, the C1858T (Lyp) polymorphism of the protein tyrosine phosphatase non-receptor type 22 (PTPN22) gene alters the function of T cells but also of B cells in innate and adaptiv...
Article
The P2×7 receptor (P2×7r) is expressed in innate immune cells (e.g. monocyte/macrophages), playing a key role in IL-1β release. Since innate immune activation and IL-1β release seem to be implicated in Behçet's disease (BD), a systemic immune-inflammatory disorder of unknown origin, we hypothesized that P2×7r is involved in the pathogenesis of the...
Article
Full-text available
Autoimmune diseases are a heterogeneous group of disorders affecting different organs and tissues whose incidence are increasing worldwide. New tools, such as genome-wide association studies, have provided evidence for new susceptibility loci and candidate genes in the disease process including common susceptibility genes involved in the immunologi...
Article
The incidence of autoimmune pathologies is increasing worldwide. This has stimulated interest on their etiopathogenesis, caused by a complex interaction of genetic and environmental factors. With the advent of genome-wide linkage, candidate gene and genome wide association studies, risk polymorphisms in autophagy-related genes were discovered in se...
Article
Full-text available
Autoimmune diseases represent a heterogeneous group of conditions whose incidence is increasing worldwide. This has stimulated studies on their etiopathogenesis, derived from a complex interaction between genetic and environmental factors, in order to improve prevention and treatment of these disorders. The relevance of T regulatory cells and of th...
Article
The pathogenesis of autoimmunity derives from a complex interaction of genetic and environmental factors. Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy is a rare autosomal recessive disease caused by mutations in the autoimmune regulator (AIRE) gene. AIRE gene variants and, in particular, heterozygous loss-of-function mutations wer...
Article
Autoimmune diseases represent a heterogeneous group of conditions whose incidence is increasing worldwide. This has stimulated studies on their etiopathogenesis, derived from a complex interaction between genetic and environmental factors, in order to improve prevention and treatment of these diseases. An increasing amount of epidemiologic investig...
Article
Full-text available
Cannabinoids modulate fibrogenesis in scleroderma. Ajulemic acid (AjA) is a non-psychoactive synthetic analogue of tetrahydrocannabinol that can bind the peroxisome proliferator-activated receptor-γ (PPAR-γ). Recent evidence suggests a key role for PPAR-γ in fibrogenesis. To determine whether AjA can modulate fibrogenesis in murine models of sclero...
Article
Systemic sclerosis (SSc) is a connective tissue disease characterised by exaggerated collagen deposition in the skin and visceral organs. Adenosine A2A receptor stimulation (A2Ar) promotes dermal fibrosis, while the cannabinoid system modulates fibrogenesis in vitro and in animal models of SSc. Moreover, evidence in central nervous system suggests...

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