Elena Dadami

Elena Dadami
University of Thessaly | UTH · Τμήμα Βιοχημείας και Βιοτεχνολογίας

PhD

About

15
Publications
5,754
Reads
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400
Citations
Additional affiliations
October 2011 - present
RLP AgroScience
Position
  • PostDoc Position
Description
  • Endogene-resembling transgenes, Viroids and RNA silencing, Suppressors of silencing, artificial miRNAs
Education
September 2007 - August 2011
University of Crete, IMBB-FORTH
Field of study
  • Molecular Biology and Biotechnology
September 2005 - September 2007
University of Crete, IMBB-FORTH
Field of study
  • Molecular Biology and Biomedicine
September 2001 - September 2005
University of Crete, IMBB-FORTH
Field of study
  • Biology

Publications

Publications (15)
Preprint
Full-text available
A growing body of evidence suggests that RNA interference (RNAi) plays a pivotal role in the communication between plants and pathogenic fungi, where a bi-directional cross-kingdom RNAi is established to the advantage of either the host or the pathogen. Similar mechanisms acting during plant association with non-pathogenic symbiotic microorganisms...
Poster
Full-text available
So far, biotechnological RNAi approaches in plants have been based on RNAi-inducing transgenes and/or transgenic plants. Here, we describe novel methods for the exogenous delivery in plants of RNA molecules having the potential to trigger RNAi in a transgene-free manner.
Chapter
In plants, RNA silencing refers to a network of pathways where small RNAs are associated with gene regulation and the defense against foreign nucleic acids including transgenes, viruses, and viroids. Replicating viroids recruit host RNA silencing machinery and trigger the production of abundant viroid-derived small interfering RNAs. Here, the recen...
Article
Full-text available
In this report we describe a method for the delivery of small interfering RNAs (siRNAs) into plant cells. In vitro synthesized siRNAs that were designed to target the coding region of a GREEN FLUORESCENT PROTEIN (GFP) transgene were applied by various methods onto GFP-expressing transgenic Nicotiana benthamiana plants to trigger RNA silencing. In c...
Article
Full-text available
RNA-directed DNA methylation (RdDM) in plants has been extensively studied, but the RNA molecules guiding the RdDM machinery to their targets need to be yet characterized. It is unclear whether these molecules require full complementarity with their target. In this study, we have generated Nicotiana tabacum (Nt) plants carrying an infectious Tomato...
Article
Full-text available
In plants, Potato spindle tuber viroid (PSTVd) replication triggers post-transcriptional gene silencing (PTGS) and RNA-directed DNA methylation (RdDM) of homologous RNA and DNA sequences, respectively. PTGS predominantly occurs in the cytoplasm, but nuclear PTGS has been also reported. In this study, we investigated whether the nuclear replicating...
Article
Full-text available
Viroids are non-encapsidated, non-coding, circular, single-stranded RNAs (ssRNAs). They are classified into the families Pospiviroidae and Avsunviroidae, whose members replicate in the nucleus and chloroplast of plant cells, respectively. Viroids have a wide host range, including crop and ornamental plants, and can cause devastating diseases with s...
Article
Full-text available
In plants, endogenes are less prone to RNA silencing than transgenes. While both can be efficiently targeted by small RNAs for post-transcriptional gene silencing (PTGS), generally only transgene PTGS is accompanied by transitivity, RNA-directed DNA methylation (RdDM) and systemic silencing. In order to investigate whether a transgene could mimick...
Article
Full-text available
Abstract In eukaryotes, DNA methylation refers to the addition of a methyl group to the fifth atom in the six-atom ring of cytosine residues. At least in plants, DNA regions that become de novo methylated can be defined by homologous RNA molecules in a process termed RNA-directed DNA methylation (RdDM). RdDM was first discovered in viroid-infected...
Article
Full-text available
In plants, transgenes are generally more sensitive against RNA silencing than endogenes are. In this study, we generated a transgene that structurally mimicks an endogene. It is composed of endogenous promoter, 5' UTR, introns, 3' UTR and terminator elements. Our data revealed that, in contrast to a conventional transgene, an endogene-resembling tr...
Article
Full-text available
In plants, RNA-directed DNA methylation (RdDM) can target both transgene promoters and coding regions/gene bodies. RdDM leads to methylation of cytosines in all sequence contexts: CG, CHG and CHH. Upon segregation of the RdDM trigger, at least CG methylation can be maintained at promoter regions in the progeny. So far, it is not clear whether codin...
Article
Full-text available
Agroinfiltration is a very fast and powerful method to express in planta any sequences in a transient fashion. Agroinfiltration has proven very useful for the overexpression of proteins in the infiltrated zone when a short-term effect can be informative. However, it has been a real success story in the induction of local and eventually systemic sil...