El-Sayed Salama

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El-Sayed verified their affiliation via an institutional email.

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• PhD, Earth Resources & Environmental Engineering, Hanyang Uni. Korea
• Professor (Full, Level 4) at Lanzhou University

Biochar is generated from plants (crop residues) and animal (bone waste) sources for environmental applications. However, the effects of plant-based biochar (PBC) and animal bone waste-derived biochar (ABWBC) in wastewater treatment (WWT), soil remediation, biofuel production, and polymer fillings have not been fully covered. Thus, this review comp...

Water quality is essential to prevent non-communicable diseases (NCDs). Low-water quality induced by contamination from natural and anthropogenic activities can lead to NCDs that affect human health. Previous studies have reported several water- and food-borne diseases. However, the direct and indirect relationships between low-water quality and NC...

Antibiotic resistance is a global health problem driven by the irrational use of antibiotics in different areas (such as agriculture, animal farming, and human healthcare). Sub-lethal concentrations of antibiotic residues impose selective pressure on environmental, plant-associated, and human microbiome leading to the emergence of antibiotic-resist...

Biocomponents (such as lipids) accumulate in oleaginous microorganisms and could be used for renewable energy production. Oleaginous microbes are characterized by their ability to accumulate high levels of lipids, which can be converted into biodiesel. The oleaginous microbes (including microalgae, bacteria, yeast, and fungi) can utilize diverse su...

Bacterial therapy for colorectal cancer (CRC) represents a burgeoning frontier. The probiotic Limosilactobacillus fermentum GR-3, derived from traditional food “Jiangshui”, exhibited superior antioxidant capacity by producing indole derivatives ICA and IPA. In an AOM/DSS-induced CRC mouse model, GR-3 treatment alleviated weight loss, colon shorteni...

Bisphenol AP (BPAP), an analog of BPA and endocrine disrupter, is increasingly being detected in water, signaling its rise as an environmental contaminant similar to BPA, which is known for its health implications. This study investigated the ecotoxicity of BPAP in four freshwater microalgae (Chlorella sorokiniana, Chlamydomonas mexicana, Scenedesm...

Plastics are one of the essential human needs and their production expanded from 2.00 million tons (Mt) in 1950 to 459.75 Mt in 2019. However, its manufacture inevitably generates large amounts of plastic wastes (PWs), which are harmful to the environment and human health. In this review, the history of plastic development since the 1860s along wit...

This study showed the effects of prepared chicken bone-derived biochar (CB) and magnetic chicken bone-derived biochar (MCB) at various ratio (2-12 g/L) on anaerobic co-digestion (AcoD) of corn straw (CS) and cow dung (CD). The physiochemical characterization of CB, MCB, and lignocellulosic biomass (including CS and CD) was analyzed. Biogas producti...

Scope: Gut microbiota (GM) dysbiosis and dysregulated bile acids (BAs) metabolism have been linked to ulcerative colitis (UC) pathogenesis. The possibility of utilizing live probiotics with a defined BAs‐metabolizing capability to modify the composition BAs for UC treatment remains unexplored. Methods and results In this study, Strain GR‐4 is sour...

Addition of a single additive (such as biochar or calcium) in anaerobic digestion has been studied previously. However, the effect of combined additives in anaerobic digestion (including fat, oil, and grease) has not been well-investigated yet. In this study, the biogas/biomethane, lipid degradation, and volatile fatty acids were investigated under...

Dear All, We would like to invite you for contribution in our Elsevier book titled by "Artificial intelligences (AI) in biofuels production", ISBN: 9780443267185. Technologies (such as anaerobic digestion, fermentation, pyrolysis, microbial fuel cells, and transesterification) have been widely used for conversion of environmental waste and pollutio...

Wastewater treatment plants (WWTPs) usually contain microplastics (MPs) due to daily influents of domestic and municipal wastewater. Thus, the WWTPs act as a point source of MPs distribution in the environment due to their incapability to remove MPs completely. In this study, MPs occurrence and distribution in anaerobic sludge from WWTPs in differe...

The Green Revolution of the mid-20th century transformed agriculture worldwide and has resulted in environmental challenges. A new approach, the Second Green Revolution, seeks to enhance agricultural productivity while minimizing negative environmental impacts. Plant microbiomes play critical roles in plant growth and stress responses, and understa...

High carbohydrate content of seaweeds (SWs) biomass makes them a potential feedstock for bioethanol production. Improvement of the conversion efficiency from polysaccharide to fermentable sugar during the pretreatment process is helpful to enhance the bioethanol yield. In this study, the response surface methodology (RSM) was used to optimize the t...

Seaweeds (SWs) are marine primitive non-flowering photosynthetic macroalgae that occur in tidal regions of seas and oceans. SWs contain various macromolecules (including carbohydrates, proteins, and lipids) which can be used for different applications. In this study, SWs species (Chondrus sp. GEEL-19 and B. gelatinae GEEL-20) were collected from th...

Microplastics (MPs) are introduced to the environment upon the breaking down of the big plastic wastes into small particles. MPs then enter the wastewater treatment plants (WWTPs) from domestic, municipal, and industrial wastewater. Primary, secondary, and tertiary units are the main stages for the treatment of wastewater. Large solids in the waste...

The presence of harmful heavy metals (HMs) in the aquatic environment can damage the environment and threaten human health. Traditional remediation techniques can have secondary impacts. Thus, more sustainable approaches must be developed. Microalgae have biological properties (such as high photosynthetic efficiency and growth), which are of great...

Worldwide, more than 400 million tons of plastic are produced annually. These plastics break down into small particles and those with a length of < 5 mm are called microplastics (MPs). The World Health Organization (WHO) has identified MPs as an emergency pollutant for the environment and human health. MPs hold toxic chemicals (such as plasticizer...

Wastewater is available medium for microalgal growth due to the presence of macro- and micro-nutrients. However, untreated wastewaters have high concentration of pollutants, chemical oxygen demand (COD), and toxicity which are not favorable for microalgae growth. Thus, mixing different wastewater to balance the nutrient availability might be a suit...

Phenanthrene (PHE) is a polycyclic aromatic hydrocarbons (PAHs) derived from natural geological events and agro-petrochemical industries. The improper disposal of PHE causes a threat to environmental health. Thus, the current study was designed to screen and identify various PHE-degrading bacteria from activated sludge (AS). The complete characteri...

Objective: The present study aims to investigate the variations in dental caries (DC) related microbiome abnormality and metabolomics shift in children. Design: The patients were divided into two groups healthy control (C) and highly affected DC children based on inclusion and exclusion criteria. Saliva samples were collected and used for the ta...

Exopolysaccharides (EPS) are bioactive metabolites with high molecular weight and are produced by several microorganisms such as endophytic fungi. Thus, the aim of the present study was designed to explore the biosynthesis and characteristics of EPS by the endophytic fungus Neopestalotiopsis clavispora AUMC15969 and then investigate some environmen...

Enhancement of growth rates and biochemical composition of marine microalgae are necessary for biofuel generation and other applications. Thus, the aim of the current review is to collect the recently published data on marine microalgae. Followed by cultivation conditions (such as media, light, temperature, pH, and cultivation time) of marine mi...

In this study, freshwater microalga (Tetradesmus obliquus GEEL-11) was cultured in municipal wastewater supplemented with various NaCl concentrations (0–150 mM). The microalgal growth kinetics, morphological changes, nutrient removals, salinity change, fatty acids (FAs) composition, and biodiesel quality were investigated. Maximum microalgal growth...

This study aimed to design an algorithm that could support the decision-making of agencies and operators responsible for environmental impact assessment (EIA) of aquatic environments in South Korea. The study provided background information on the basics of assessment, including the review standards of review agencies and review opinions for major...

Gluten accumulation damages the proximal small intestine and causes celiac disease (CeD) which has not been effectively treated except by using a gluten-free diet. In this study, strain Bacillus subtilis LZU-GM was isolated from Pakistani traditional fermented sourdough and could degrade 73.7% of gluten in 24 hours in vitro. Strain LZU-GM was emplo...

Aerobic granular sludge (AGS) is an effective antibiotic wastewater treatment technology. However, although sulfonamide antibiotics (SAs) and microplastics (MPs) are frequently and simultaneously detected, their combined effects on AGS remain unclear. Herein, the effects of a combination of polystyrene (PS) and sulfamethoxazole (SMX) on the granula...

Aims: Preliminary studies have identified the use of probiotics as a potential treatment strategy against colorectal cancer (CRC). However, natural probiotics lack direct tumor-targeting and tumor-killing activity in the intestine. This study aimed to construct a tumor-targeting engineered probiotic to combat CRC. Main methods: Standard adhesion...

Bisphenols (BPs) are recognized as emerging contaminants because of their estrogenic properties and frequent occurrence in environmental matrices. Here, we evaluated the toxic effects of five common BPs on freshwater microalga Chlamydomonas mexicana and removal of the BPs by the alga. Bisphenols -AF (BPAF), -B (BPB), and -Z (BPZ) (96 h, EC50 1.78-1...

Serum uric acid elevation has been found in long-term nickel (Ni) exposure occupational workers, but the mechanism is unclear. In this study, the relationship between Ni exposure and uric acid elevation was explored in a cohort of 109 participants composed of a Ni-exposed workers group and a control group. The results showed that Ni concentration (...

Availability of biomass makes biofuel from bioresources more feasible. Recently, algal biomass (AB) and lignocellulosic biomass (LB) have been widely used in bioelectrochemical systems (BESs) for bioelectricity and biohydrogen production. However, no review provides the current scenario of bioelectricity and biohydrogen generation from various biom...

The isolation, screening, and diversified biocomponent analysis of microalgae species is essential for categorizing its potential applications. In the present study, several microalgal species were isolated from freshwater samples collected from three geographically distant cities, Lanzhou Shi Chuang Xian Dai Cheng in Gansu Province (103.75 E, 36.0...

Microalgae-based biotechnologies have been a promising approach for the efficient treatment of textile wastewater. Numerous studies have demonstrated the potential of such approaches toward the treatment of real textile effluent. Therefore, in this chapter, we have discussed the efficiency and degradation mechanisms of microalgae-based biotechnolog...

Wastewater treatment by microalgae is an eco-friendly and sustainable method for pollutant removal and biomass generation. Microalgae production under abiotic stress (such as salinity/salt stress) has an impact on nutrient removal and fatty acid accumulation. In this study, a freshwater microalgal strain (Desmodesmus communis GEEL-12) was cultured...

The efficiency of anaerobic digestion (AD) chiefly relies on substrate composition and microbial structure. The lipidic substrates such as waste cooking oils (WCOs) have high biogas potential. The biogas production from WCOs can be increased by active microbes with simultaneous alleviation of environmental contamination. Thus, this study explored t...

The secondary metabolites produced by algae (including macroalgae and microalgae) possess several human health benefits. The bioactive compounds in algae have potential to be used in the medical and pharmaceutical industries for drug discovery. The rapid development of genetic tools and omics approaches has extended the understanding of algal metab...

The presence of heavy metals (HMs) in aquatic ecosystems is a universal concern due to their tendency to accumulate in aquatic organisms. HMs accumulation has been found to cause toxic effects in aquatic organisms. The common HMs-induced toxicities are growth inhibition, reduced survival, oxidative stress, tissue damage, respiratory problems, and g...

The ability of Pseudomonas turukhanskensis GEEL-01 to degrade the phenanthrene (PHE) was optimized by response surface methodology (RSM). Three factors as independent variables (including temperature, pH, and inoculum) were studied at 600 mg/L PHE where the highest growth of P. turukhanskensis GEEL-01 was observed. The optimum operating conditions...

The global energy demand and need for sustainable sources are expected to rise in the coming years. The second-generation bioethanol production from lignocellulosic biomass (LB) can sustain the energy demand. Thus, this review comprehensively discussed the suitable feedstock selection and different pretreatment progress (including mechanical/therma...

Recently, a lot of research work has been performed on microalgal-assisted microbial fuel cells (MFCs) for electricity generation, wastewater treatment, and biomass production. However, a comprehensive investigation on the insight of microalgal-assisted MFCs along with a deep discussion on the application of molecular biology approaches has not bee...

Cancer is a vital barrier to increase the life expectancy and the foremost cause of death globally. The initial diagnosis and proper management of cancer can expand the survival rate of individuals. This review provides an in-depth investigation of cancer causes symptoms, types of cancer, and worldwide distribution of cancer. The relation between n...

The dibutyl phthalate (DPB) is an emerging plasticizer contaminant that disrupts the biological processes of primary producers, especially phytoplankton. In this study, two microalgal species (Chlorella sp. GEEL-08 and Tetradesmus dimorphus GEEL-04) were exposed to various concentrations of DBP extending from 0 to 100 mg/L. The growth kinetics, N-n...

The biogas production (BP), volatile fatty acids (VFAs), microbial communities, and microbes’ active enzymes were studied upon the addition of biochar (0–1.5%) at 6% and 8% slaughterhouse waste (SHW) loadings. The 0.5% biochar enhanced BP by 1.5- and 1.6-folds in 6% and 8% SHW-loaded reactors, respectively. Increasing the biochar up to 1.5% caused...

The recycling of phytoremediated biomass produce in the process of phytoremediation is a challenging issue worldwide. The disposal of contaminated plant biomass has been recycled in composting, burning, pyrolysis, phytomining, and biosynthesis of nanoparticles. These techniques have the disadvantage of impairing or even destroying the biological ec...

Exposure to heavy metals (HMs) is a threat to human health. Although probiotics can detoxify HMs in animals, their effectiveness and mechanism of action in humans have not been studied well. Therefore, we conducted this randomized, double-blind, controlled trial on 152 occupational workers from the metal industry, an at-risk human population, to ex...

Two types of plastic carriers low-density polyethylene (LDPET) and high-density polyethylene (HDPET) were used as a support material for biofilm formation during anaerobic co-digestion of agricultural wastes. LDPET and HDPET were added separately to different reactors containing binary substrates: corn straw and cauliflower leaves (G 1), corn straw...

Purpose Microalgae show growth and fatty acids (FAs) accumulation up to a certain level which is dependent on species and type of cultivation conditions. Application of abiotic stress on microalgae can induce nutrient uptake and enhance biocomponent accumulation. Methods In the current study, freshwater microalgae (Chlorella sorokiniana GEEL-01 an...

The protein-rich substrates (PRSs) are generated extensively as stated by the Food and Agriculture Organization (FAO) of the United Nations. Recently, enormous research data is available on the use of PRSs for biomethane and biogas production. However, the complete utilization of PRSs from energy and material recovery has not been comprehensively r...

Recalcitrancy in microplastics (MPs) contributes to white pollution. Bioremediation can remove MPs and facilitate environmental sustainability. Although recent studies have been conducted on the interaction of algae and MPs, the role of algae in MP removal with the simultaneous implementation of ‘omics studies has not yet been discussed. Here, we r...

This study aims to determine the environmental impacts and feasibility of optimizing the production of phenolic-rich bio-oil, via switchgrass microwave catalytic pyrolysis. K3PO4 (Tripotassium phosphate) was used as the catalyst, at different temperatures, throughout this life cycle assessment (LCA) study. Results were compared with non-catalytic m...

Seaweeds are a potential source for the extraction of bioactive compounds, which are beneficial to human health. Therefore, the aim of the present study was to comprehensively characterize Padina australis GEEL-18 biomass collected from Luhuitou small east China sea. The identification, biochemical composition, physicochemical, and spectroscopic an...

Isolation and screening of microalgal species from natural habitats should be a continues work to discover effective species for biofuel generation and wastewater treatment. In the current study, microalgal species namely Tetradesmus dimorphus GEEL-04 and Desmodesmus asymmetricus GEEL-05 were isolated, identified, and assessed for growth kinetics,...

Wastewater utilization as a medium for microalgal cultivation have copious simultaneous perquisites such as removal of nutrients (80–100%), heavy metal removal, carbon dioxide (CO2) sequestration from atmosphere (1.83 kg CO2 kg−1 biomass), and high biomass production for biofuel generation (40–50% higher than crops feedstock). Municipal, industrial...

Anaerobic digestion (AD) encounters several challenges such as low digestibility of substances and biomethane production. Recently, extensive research has been conducted on biochar (BC) inclusion in the AD to mitigate inhibitors, promotes process stability, and increase biomethane production. However, a comprehensive review enclosing in-depth the e...

Chitin (CT) and its deacetylated derivative, chitosan (CS), are globally available in large quantities mostly found in marine and freshwater ecosystems as a waste generated from the exoskeletons of seafood. Thus, the motivation of present review is to identify the global waste generation of CT/CS and its evaluation as a potential feedstock for vari...

Microplastics (MPs) remediation via microalgae could be a prospective strategy to address MPs pollution concerns. In this study, Chlorella sp. GEEL-08 was exposed to different gradient concentrations ranging from 0 to 200 mg L⁻¹ of polyvinyl chloride (PVC0.2μm). Microalgal growth, total nitrogen (TN), total phosphorus (TP), and cations (Cu, Zn, Na,...

Selenium levels have a critical impact on livestock and poultry, and selenium nanoparticles (SeNPs) have shown significant efficiency in the supplementation. This study identified a high-efficiency selenite reductase, SerV01, in Staphylococcus aureus LZ-01, which can convert Se2O32- to SeNPs. Subsequently, SerV01 was applied into the intestine of t...

The widespread distribution of organic and inorganic pollutants in water resources have increased due to rapid industrialization. Rhizospheric zone-associated bacteria along with endophytic bacteria show a significant role in remediation of various pollutants. Metaomics technologies are gaining an advantage over traditional methods because of their...

β-oxidation is a well-known pathway for fatty acid (FA) degradation. However, the wide range of feedstocks, their intermediates, and complex microbial networks involved in anaerobic digestion (AD) make β-oxidation unclear during lipid digestion having a variety of long-chain fatty acids (LCFAs). Here, we demonstrated the detailed metabolic pathway...

Procambarus clarkii (crayfish) accumulates a high concentration of Arsenic (As) from the aquatic environment and causes considerable human health risks. In this study, Limosilactobacillus fermentum GR-3 strain was isolated from “Jiangshui” and applied for As(III) adsorption and antioxidant abilities. Strain GR-3 removed 50.67% of 50 mg/L As(III) an...

The global trend of seaweeds production and their biomass utilization for biofuels generation is increased due to the high carbohydrates and proteins content. Anaerobic digestion (AD) is a promising technology to convert organic compounds of seaweed biomass into biogas. However, a comprehensive review enclosing the biomethane potential, process par...

The current study aimed to investigate the effect of periodontitis and long-term heavy metal (HM) exposure on the salivary microbiome. The patients were divided into four groups as Wu Wei control (WWC) group involved healthy individuals, Wu Wei periodontitis (WWP) patients having periodontitis, Jing Chang with metal pollution periodontally healthy...

Waste oils are generated in massive amounts and can be used for biodiesel, biogas, and biopolymers production due to their high lipid content. In this study, the potential applications of pig-cooked waste oil (PCW) and vegetable-cooked waste oil (VCW) were evaluated by proximate and ultimate analyses. The elemental composition was also determined b...

Biodiesel production from edible sources faces several challenges such as food security and cost issues. Waste cooking oils (WCOs) can be an alternative feedstock due to their large production. The comprehensive characterization of WCOs has been rarely reported in previous studies. In this study, six different WCOs including chicken oil (CO), fat,...

A microbial fuel cell (MFC) based biosensor was designed to monitor volatile fatty acids (VFAs) in anaerobic digestion (AD) to control substrate feeding. The VFAs concentrations 7-40.83 mM showed a linear relationship with biosensor voltage (18-67 mV) indicating that voltage < 18 mV was due to low organic matter. Likewise, VFAs concentration lower...

Biochar addition could facilitate the microbial communities’ abundance and degradation of lignocellulosic biomass in anaerobic digestion (AD). The effect of biochar from walnut (Juglans regia L.) shell has not been studied in AD so far. Therefore, different concentrations of walnut shell biochar (WNSB) (1–8 g/L) were added to the AD of lignocellulo...

Heavy metals (HMs) contaminated fish is a threat to humans when consumed. Dietary probiotics have evolved as a successful HMs removal approach. In this study, probiotics Enterococcus (EC) sp. and Lactococcus (LC) sp. were evaluated for toxicity alleviation and gut microbiota maintenance in Cyprinus carpio (single and combined approach) on Cr, Cd, a...

Lack of process control between the two stages of a combined microbial fuel cell-membrane bioreactor (MFC-MBR) system limits its application in wastewater treatment due to membrane fouling and high energy consumption. In this study, a two-stage MFC-MBR integrated system was established to investigate the impact of incorporating process control on p...

In this study, a novel and sustainable approach was used to synthesize nitrogen-doped carbon dots (NCDs) from the waste biomass of Poa Pratensis (Kentucky bluegrass (KB)) by a facile hydrothermal method. The prepared KBNCDs were subjected to various characterization techniques, including X-ray diffraction, X-ray photoelectron spectroscopy, transmis...

Seaweeds from three different phyla including Sargassum thunbergii GEEL-15 (Phaeophyta), Mastocarpus stellatus GEEL-16 (Rhodophyta), and Ulva sp. GEEL-17 (Chlorophyta) were screened, identified, and evaluated for their potential applications. Characterizations including analysis of the bio-components (carbohydrates, proteins, and lipids), proximate...

Bauxite residue (BR) is a highly alkaline type of solid waste generated by the aluminum industry that poses a significant environmental risk upon disposal. However, BR is abundant in metals, especially iron, that offer the desired catalytic activity for microwave pyrolysis. Thus, this study aimed to use BR as a low-cost microwave absorber and catal...

The commercial feasibility of energy-efficient conversion of highly concentrated microalgal suspensions to produce high-titer biofuels is a major bottleneck due to high energy consumption. Herein, high-titer biofuels (bioethanol, higher-alcohols, and biodiesel) were generated from carbohydrate-rich Chlamydomonas mexicana and lipid-rich Chlamydomona...

Biomethanation of carbohydrates (e.g., lignocellulosic biomass) and lipids (e.g., waste oils) has been well studied. However, investigations on the biomethanation of protein-rich biowastes (PRBs) and associated microbial communities have not been reported. This review summarizes the challenges in the metabolic process of anaerobic digestion of PRBs...

Biofiltration (BF) facilitates the removal of organic and inorganic compounds through microbial reactions. Water is one of the most important elements in biotrickling filters that provides moisture and nutrients to microbial biofilms. The maintenance of proper trickle watering is very critical in biotrickling filtration because the flow rate of the...

The slaughterhouse waste (SHW) contains high organics which makes SHW a feasible feedstock for anaerobic digestion (AD). The present study systematically assessed the microbiome response and biomethanation along with the production of volatile fatty acids (VFAs) and ammonia under 2%, 4%, 6%, and 8% (w v⁻¹) loadings of SHW in AD. The optimum loading...

Anaerobic digestion (AD) is a biological process, encompasses the treatment and stabilization of complex organic matter to extract renewable biomethane fuel through microbial interaction in the digester. The main aim of the current study is to investigate the microbial community of anaerobic digestion sludge (ADS), collected at different periods fr...

Eutrophication is a global concern that has severely deteriorated the aquatic ecosystems. This phenomenon has impacted the freshwater bodies (such as lakes and rivers) across the world due to the nutrients (mainly N and P) arising from point and non-point pollution sources. Nutrient enrichment induces cyanobacteria formation, hypoxia, and release o...

Lignocellulosic biomass is a highly available and suitable candidate for biogas/biomethane production. However, high concentration of recalcitrant lignin is the major obstacle in successful anaerobic digestion (AD). The main aim of this review is to highlight the applications and challenges of lignocellulosic biomass in AD from the recent reports....

Emergence of pollutants in wastewater, expensive cultivation of microalgae, and difficulties in industrial scale production are the main challenges for successful coupling of microalgae with wastewater. Nitrogen, carbon, and phosphorus in wastewater are deliberately consumed by microalgae and cyanobacteria for their growth and could act as green te...

Anaerobic digestion, a promising technology for waste utilization and bioenergy generation, is a suitable approach to convert the shrimp waste to biomethane, reducing its environmental impact. In this study, shrimp chaff (SC) was co-digested corn straw (CS), wheat straw (WS), and sugarcane bagasse (SB). In co-digestion, SC enhanced biomethane produ...

Pharmaceutical wastewater is usually complex and highly toxic, mostly containing antibiotics. The conventional activated sludge technology is unsatisfactory in the treatment of pharmaceutical wastewater owing to the impact of antibiotic toxicity. In this study, a novel whole-cell biocatalyst that can accelerate the degradation of antibiotics was de...

In this study, Moringa oleifera seeds (MOS) have been used as a biosorbent, to remove Congo red (CR) dye from the aqueous medium. The feasibility of using MOS as biosorbent was assessed by measuring adsorption, kinetic, and thermodynamic parameters. The removal of dye was affected by different factors, including initial dye concentration, adsorbent...