Edward P Kolodziej

Edward P Kolodziej
University of Washington Seattle | UW · Department of Civil and Environmental Engineering

PhD

About

53
Publications
8,808
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,975
Citations
Additional affiliations
September 2014 - present
University of Washington (Tacoma/Seattle)
Position
  • Professor (Associate)
January 2007 - August 2014
University of Nevada, Reno
Position
  • Professor (Associate)
August 1999 - December 2006
University of California, Berkeley
Position
  • Graduate Student/Post Doc

Publications

Publications (53)
Preprint
Catecholamines, such as L-norepinephrine (L-NE), are naturally present in the human gut and are discharged into the sewage. The bioactivity of L-NE can significantly alter the speciation and function of the microbial community by stimulating bacterial growth and producing H 2 O 2 . The accompanying changes in intracellular metabolism could signific...
Article
Chemical contamination is an increasingly important conservation issue in urban runoff-impacted watersheds. Regulatory and restoration efforts typically evaluate limited conventional parameters and pollutants. However, complex urban chemical mixtures contain hundreds to thousands of organic contaminants that remain unidentified, unregulated, and po...
Article
Effective management of contaminated sites requires differentiating and deconvoluting contaminant source impacts in complex environmental systems. The existing source apportionment approaches that use targeted analyses of preselected indicator chemicals are limited whenever target analytes are below the detection limits or derived from multiple sou...
Article
Full-text available
Tire tread wear particles (TWP) are increasingly recognized as a global pollutant of surface waters, but their impact on biota in receiving waters is rarely addressed. In the developed U.S. Pacific Northwest, acute mortality of adult coho salmon (Oncorhynchus kisutch) follows rain events and is correlated with roadway density. Roadway runoff experi...
Article
Current aquatic toxicity assessments usually focus on targeted analyses coupled with toxicity testing to determine the impacts of complex mixtures on aquatic organisms. However, based on this approach alone, it is sometimes difficult to explain observed toxicity from the selected chemical analytes. Recent analytical advances such as high-resolution...
Article
In U.S. Pacific Northwest coho salmon (Oncorhynchus kisutch), stormwater exposure annually causes unexplained acute mortality when adult salmon migrate to urban creeks to reproduce. By investigating this phenomenon, we identified a highly toxic quinone transformation product of N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine) (6PPD), a globally...
Article
Diffuse pollution in urban receiving waters often adversely impacts both humans and ecosystems. Identifying such pollution sources is challenging and limits the effectiveness of management actions intended to reduce risk. Here, we evaluated the use of nontarget analysis via high-resolution mass spectrometry (HRMS) to develop chemical fingerprints/s...
Article
Full-text available
Photolysis of trenbolone acetate (TBA) metabolites in the presence of various nitrogen-, sulfur-, or oxygen-containing nucleophiles (e.g., azide, ammonia, or thiosulfate, respectively) results in rapid (half-lives ~20-60 min), photochemically induced nucleophile incorporation across the parent steroid’s trienone moiety. The formation of such nucleo...
Article
Stormwater runoff clearly impacts water quality and ecological health of urban receiving waters. Subsequent management efforts are often guided by conceptual models of contaminant “first flushes”, defined by disproportionate concentrations or mass loads early in the storm hydrograph. However, studies examining the dynamics of contaminant transport...
Article
This study used suspect and non-target screening with high-resolution mass spectrometry to characterize the occurrence of contaminants of emerging concern (CECs) in the nearshore marine environment of Puget Sound (WA, USA). In total, 87 non-polymeric CECs were identified; those confirmed with reference standards (45) included pharmaceuticals, herbi...
Article
Pollutants transported in urban stormwater runoff induce pervasive water quality degradation in receiving waters. To accurately characterize stormwater quality and treatment system performance across the range of possible contaminant characteristics, comprehensive multi-residue analytical methods are necessary. Here, we developed a solid-phase extr...
Article
High resolution mass spectrometry (HRMS) analyses provide expansive chemical characterizations of environmental samples. To date, most research efforts have developed tools to expedite labor- and time-intensive contaminant identification efforts. However, even without chemical identity, the richness of non-target HRMS datasets represents a signific...
Article
Full-text available
This study evaluated the sorption and transport potential of seven phototransformation products of 17α-trenbolone, 17β-trenbolone, trendione, and altrenogest, along with the parent trienone steroids in batch and column soil-water systems. In batch systems, the target solutes exhibited linear isotherms, with values for sorption coefficients (logKoc)...
Article
While studying the environmental fate of potent endocrine-active steroid hormones, we observed the formation of an intramolecular [2+2] photocycloaddition product (2) with a novel hexacyclic ring system following photolysis of altrenogest (1). The structure and absolute configuration were established by X-ray diffraction analysis. Theoretical compu...
Article
Full-text available
Organic micropollutants (OMPs) are pervasive anthropogenic contaminants of receiving waters where they can induce various adverse effects to aquatic life. Their ubiquitous environmental occurrence is primarily attributed to discharge from wastewater treatment plants due to incomplete removal by common biological wastewater treatment processes. Here...
Article
Here, we developed a novel and sensitive method for the detection and quantification of metastable trenbolone and altrenogest photoproducts in agricultural receiving waters based on solid phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Primary method analytes were seven cycloaddition or photohydration transform...
Preprint
Full-text available
Organic micropollutants (OMPs) are pervasive anthropogenic contaminants of fresh and marine waters with known potential to adversely affect aquatic life (e.g. endocrine disruption). Their ubiquitous environmental occurrence is primarily attributed to wastewater treatment plant discharges following their incomplete removal by common biological treat...
Article
The hyporheic zone (HZ), located at the interface of surface and groundwater, is a natural bioreactor for attenuation of chemical contaminants. Engineered HZs can be incorporated into stream restoration projects to enhance hyporheic exchange, with flowpaths optimized to promote biological habitat, water quantity, and water quality improvements. Des...
Article
Associate Editor Ed Kolodziej introduces the Environmental Science: Processes & Impacts Underappreciated Science collection.
Article
Urban stormwater is a major threat to ecological health, causing a range of adverse, mostly sublethal effects. In western North America, urban runoff is acutely lethal to adult coho salmon (Oncorhynchus kisutch) that spawn each fall in freshwater creeks. Although the mortality syndrome is correlated to urbanization and attributed to road runoff con...
Article
A first-of-its-kind, gas-tight MD system was designed to provide insight into the dynamic transport of semi-volatile contaminants over time by closing the mass balance for each contaminant. Testing with non-volatile ions was used to confirm the integrity of the test system and sampling procedures. Non-volatile contaminants had consistently high rej...
Article
Full-text available
Potent trienone and dienone steroid hormones undergo a coupled photohydration (in light)-thermal dehydration (in dark) cycle that ultimately increases their environmental persistence. Here, we studied the photolysis of dienogest, a dienone progestin prescribed as a next-generation oral contraceptive, and used high resolution mass spectrometry and b...
Article
Guest editors Edward Kolodziej, Kyungho Choi, Ruth Marfil-Vega and Bryan Brooks introduce the “Bioanalytical tools for water and sediment quality assessment” themed issue of Environmental Science: Processes & Impacts .
Article
Untreated urban stormwater runoff contributes to poor water quality in receiving waters. The ability to identify toxicants and other bioactive molecules responsible for observed adverse effects in a complex mixture...
Article
Full-text available
Glucocorticoid (GC) release into the environment has led to widespread detection of glucocorticoid receptor (GR) activity in water resources that has been shown to persist throughout conventional and some advanced wastewater treatment processes. Here, we used high performance liquid chromatography, high resolution mass spectrometry and nuclear magn...
Article
Full-text available
Despite its wide use as a veterinary pharmaceutical, environmental fate data is lacking for altrenogest, a potent synthetic progestin. Here, it is reported that direct photolysis of altrenogest under environmentally relevant conditions was extremely efficient and rapid (half-life ~25 seconds). Photolysis rates (observed rate constant kobs = 2.7 ± 0...
Article
Photolysis experiments (in H2O and D2O) and quantum chemical calculations were performed to explore the pH-dependent, reversible photohydration of trenbolone acetate (TBA) metabolites. Photohydration of 17α-trenbolone (17α-TBOH) and 17β-trenbolone (17β-TBOH) occurred readily in simulated sunlight to yield hydrated products with incorporated H(+) at...
Article
Full-text available
Existing regulatory frameworks for aquatic pollutants in the United States are idealized, often lacking mechanisms to account for contaminants characterized by (1) bioactivity of both the parent and transformation products and (2) reversible transformations (that is, metastable products) driven by chemical or physical heterogeneities. Here, we mode...
Article
Trenbolone acetate (TBA) metabolites are endocrine active contaminants discharged into the aquatic environment in runoff from agricultural fields, rangelands, and concentrated animal feeding operations (CAFOs). To investigate the environmental fate of these compounds and their biotransformation mechanisms, we used inocula from a variety of differen...
Article
This work examines the fate of synthetic growth promoters (trenbolone acetate, melengestrol acetate, and zeranol) in sterilized soil systems, focusing on their sorption to organic matter and propensity for mineral-promoted reactions. In organic-rich soil matrices (e.g., Pahokee Peat), the extent and reversibility of sorption did not generally corre...
Article
Full-text available
To assess the relative ecological risks of trenbolone acetate (TBA) use in agro-ecosystems, we evaluated the spatio-temporal dynamics of TBA metabolite transport during irrigation and rainfall events. Within a pasture, TBA-implanted heifers (40 mg TBA, 8 mg estradiol) were briefly penned (24 hr) at high stocking densities (500 animal units (AU)/ha)...
Article
Environmental transformation processes, including those occurring in natural and engineered systems, do not necessarily drastically alter molecular structures of bioactive organic contaminants. While the majority of generated transformation products are likely benign, substantial conservation of structure in transformation products can imply conser...
Article
Full-text available
Although studies have evaluated the ecotoxicity and fate of trenbolone acetate (TBA) metabolites, namely 17α-trenbolone (17α-TBOH), 17β-trenbolone (17β-TBOH), and trendione (TBO), their environmental transport processes remain poorly characterized with little information available to guide agricultural runoff management. Therefore, we evaluated TBA...
Article
Animal waste from livestock farming operations (LFOs) can contain varying levels of natural and synthetic androgens and/or estrogens, which can contaminate surrounding waterways. In the present study, surface stream water was collected from six basins containing LFOs. Aqueous concentrations of 12 hormones were determined via chemical analyses. Rela...
Article
Full-text available
Several studies have documented the occurrence and fate of trenbolone acetate (TBA) metabolites in soil and water. However, considerable uncertainty still exists with respect to TBA risk in agro-ecosystems because limited data are available to quantify excretion, transformation, and leaching processes. To address these uncertainties, we used experi...
Article
Full-text available
Trenbolone acetate (TBA) is a high-value steroidal growth promoter administered to beef cattle whose metabolites are potent endocrine-disrupting compounds. We performed laboratory and field phototransformation experiments to assess the fate of TBA metabolites and their photoproducts. Unexpectedly, we observed that the rapid photohydration of TBA me...
Article
Despite the widespread use of the anabolic androgen trenbolone acetate (TBA) in animal agriculture, evidence demonstrating the occurrence of TBA metabolites such as 17β-trenbolone (17β-TBOH), 17α-trenbolone (17α-TBOH), and trendione (TBO) is relatively scarce, potentially due to rapid transformation processes such as direct photolysis. Therefore, w...
Article
Trenbolone acetate, melengestrol acetate, and zeranol are synthetic hormones extensively used as growth promoters in animal agriculture, yet despite occurrence in water and soil little is known about their environmental fate. Here, we establish the time scales and mechanisms by which these synthetic growth promoters and their metabolites (SGPMs) un...
Article
Studies demonstrate that exposure to steroid hormones in receiving waters can adversely impact reproduction of aquatic organisms. In particular, exogenous steroid hormones widely used as growth promoters in animal agriculture are of high concern, yet no gas chromatography-tandem mass spectrometry (GC/MS/MS) analytical methods for the detection of t...
Article
Metabolites of androgenic synthetic growth promoters used at confined animal feeding operations (CAFOs) pose a demonstrated ecological risk. To evaluate the transport of trenbolone acetate (TBA) metabolites from beef cattle CAFOs, rainfall simulation experiments were conducted at the University of California, Davis, research CAFO. Steroid concentra...
Article
Steroid hormones pose potential risks to fish and other aquatic organisms at extremely low concentrations. To assess the factors affecting the release of endogenous estrogenic and androgenic steroids from feedlots during rainfall, runoff, and soil samples were collected after simulated rainfall on a 14-steer feedlot under different rainfall rates a...
Article
To evaluate the occurrence and sources of compounds capable of feminizing fish in agriculturally impacted waterways of the Central Valley of California, water samples were extracted and subjected to chemical analyses as well as in vitro and in vivo measurements of vitellogenin in juvenile rainbow trout (Oncorhynchus mykiss). Among the 16 sites samp...
Article
Cattle and other livestock excrete endogenous steroid hormones, including estrogens, androgens, and progestins; therefore, allowing grazing livestock direct access to surface waters can result in the release of steroids in agricultural watersheds. Elevated concentrations of steroids are problematic because low concentrations of certain steroids can...
Article
Although wastewater-derived chemical contaminants undergo transformation through a variety of mechanisms, the relative importance of processes such as biotransformation and photolysis is poorly understood under conditions representative of large rivers. To assess attenuation rates under conditions encountered in such systems, samples from the Trini...
Article
Full-text available
The exposure and uptake of environmental estrogenic compounds have been reported in previous studies of demersal flatfish species in the central Southern California Bight (SCB), USA. The objective of this study was to evaluate the estrogenic or feminizing activity of marine sediments from the SCB by using in vivo vitellogenin (VTG) assays in male o...
Article
A suite of androgens, estrogens, and progestins were measured in samples from dairy farms, aquaculture facilities, and surface waters with actively spawning fish using gas chromatography-tandem mass spectrometry (GC/MS/ MS) to assess the potential importance of these sources of steroid hormones to surface waters. In a dairywaste lagoon, the endogen...
Article
Many fish use steroid hormones as pheromones to initiate behavioral and physiological changes during spawning. To assess the occurrence of steroid hormones with pheromonal properties in the aquatic environment and to evaluate the possibility that municipal wastewater discharges contain compounds that could affect fish reproduction by interfering wi...
Article
The effluent from municipal wastewater treatment plants contains trace concentrations of a variety of organic compounds. To assess the removal of these compounds in full-scale treatment systems and effluent-receiving waters, approaches are needed for quantifying removal rates and mechanisms. For processes that result in near complete removal of was...