
Eduardo Souza RibeiroUniversity of Guelph | UOGuelph · Animal Biosciences
Eduardo Souza Ribeiro
BVetMed, MSc, PhD
About
141
Publications
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Introduction
Additional affiliations
July 2020 - July 2020
January 2016 - June 2020
January 2015 - May 2015
Education
August 2011 - July 2015
August 2009 - July 2011
March 2004 - December 2008
Santa Catarina State University
Field of study
- Veterinary Medicine
Publications
Publications (141)
Ruminants have the ability to digest human-inedible plant materials, due to the symbiotic relationship with the rumen microbiota. Rumen microbes supply short chain fatty acids, amino acids, and vitamins to dairy cows that are used for maintenance, growth, and lactation functions. The main goal of this study was to investigate gene-microbiome networ...
The objectives of this study were to identify factors associated with prepartum DMI, evaluate the performance of linear models to estimate prepartum DMI using different classes of predictors, and investigate the consequences of different levels of prepartum DMI on transition metabolism and lactation performance. Individual feed intake of nulliparou...
The objectives of this study were to identify factors associated with the relative change in prepartum dry matter intake (RCDMI) of 273 cows fed individually, evaluate the performance of linear models to estimate RCDMI using different classes of predictors, and characterize the implications of RCDMI to transition metabolism, health, and subsequent...
Our objective was to evaluate the effects of omega-3 fatty acids (n3 FA)
supplementation on circulating metabolites and lactation performance. In a randomized controlled trial, cows (n = 104) were blocked by parity and BCS, and assigned randomly to 1 of 4 dietary treatments from 2 to 90 DIM: (1) UNSUP: unsupplemented control; (2) CaPO: calcium salt...
Our objectives were to investigate whether prepartum cytokine responses to ex vivo lipopolysaccharide (LPS) stimulation of whole blood is associated with incidence of postpartum clinical disease. Blood samples from 332 cows were collected 15 d before the expected calving and divided into 2 aliquots. One aliquot remained as an unstimulated control,...
The rumen microbiome is crucial for converting feed into absorbable nutrients used for milk synthesis, and the efficiency of this process directly impacts the profitability and sustainability of the dairy industry. Recent studies have found that the rumen microbial composition explains part of the variation in feed efficiency traits, including dry...
The rumen microbiome of dairy cows is affected by parity and diet, especially dietary changes from pre- to postpartum periods. Despite this understanding, there is limited knowledge regarding the interaction between these factors and changes in the microbiome. Here, we investigated such interactions using a deep shotgun metagenomics approach. The r...
Our objective was to evaluate the effects of complete replacement of supplementary inorganic salts of trace minerals (STM; Co, Cu, Mn, and Zn sulfates and Na selenite) by organic trace minerals (OTM; Co, Cu, Mn, and Zn proteinates and selenized yeast) on the rumen microbiome. Heifers and cows (n = 72) were enrolled at 45 ± 3 d before expected calvi...
Our objective was to evaluate the effects of short- and long-term supplementation of omega-3 fatty acids (n3 FA) on ovarian and uterine biology. Healthy Holstein cows (n = 104) were assigned to 1 of 4 dietary treatments from 2 to 90 DIM: (1) UNSUP: unsupplemented control; (2) CaPO: calcium salts of palm oil (EnerGII; Virtus Nutrition) through 90 DI...
Genetic selection has remarkably helped U.S. dairy farms to decrease their carbon footprint by more than doubling milk production per cow over time. Despite the environmental and economic benefits of improved feed and milk production efficiency, there is a critical need to explore phenotypical variance for feed utilization to advance the long-term...
It is now widely accepted that dairy cow performance is influenced by both the host genome and rumen microbiome composition. The contributions of the genome and the microbiome to the phenotypes of interest are quantified by heritability (h2) and microbiability (m2), respectively. However, if the genome and microbiome are included in the model, then...
Our objective was to investigate associations of postpartum health with serum progesterone (P4) concentrations after first artificial insemination (AI), expression of interferon-stimulated genes (ISG) in blood leukocytes, and serum pregnancy-associated glycopro-tein (PAG) during early pregnancy in dairy cows. In this observational study, Holstein c...
Our objective was to describe associations of post-partum health with pregnancy and pregnancy loss (P-LOSS) from d 19 to 40 after first postpartum artificial insemination (AI) in lactating Holstein cows. In 2 commercial dairy herds in Ontario, Canada, 468 Holstein cows were enrolled 21 ± 3 d before expected parturi-tion when body condition score (B...
The objective of this prospective observational study was to investigate associations of postpartum health with estrus detection (ED) by activity monitors and pregnancy outcomes in dairy cows. A total of 1,743 Holstein cows from 2 commercial dairy herds in Ontario, Canada were enrolled 3 wk before expected parturition and examined for health variab...
The objective was to characterize transcriptome
changes associated with elongation in bovine conceptuses
during preimplantation stages. Nonlactating Holstein
cows were euthanized 17 d after artificial insemination
(AI) and the uterine horn ipsilateral to the CL
was flushed with saline solution. Recovered conceptuses
were classified as small (1.2 to...
The objective was to characterize endometrial transcriptome
on d 17 of gestation in dairy cows according
to conceptus length. Nonlactating Holstein cows (n =
48) were euthanized 17 d after AI and the uterine horn
ipsilateral to the CL was flushed with saline solution.
Recovered conceptuses were classified as small (1.2 to
6.9 cm; n = 9), medium (10...
Our objective was to investigate the effects of dietary supplementation of omega-3 (n3) fatty acids (FA) on physiological responses to lipopolysaccharide (LPS) challenge in early postpartum cows. Healthy cows were blocked by parity and BCS and randomly assigned to 1 of 3 top-dress treatments at 2 d in milk (DIM): 1) unsupplemented control received...
Our objectives were to investigate the changes in fatty acids (FA) composition in peripheral blood plasma and leukocytes (PBL) following an intravenous (IV) infusion of lipopolysaccharide (LPS) in early postpartum cows. At 14 DIM, cows were blocked by parity and BCS and randomly assigned to one of 2 IV infusions: LPS at 0.0625 μg/ kg of BW diluted...
Our objectives were to identify factors associated with the level of
prepartum DMI and its consequences on transition metabolism and performance. Cows (n = 273) were assigned to automatic feeding bins to measure feed intake from 45 d prepartum to 98 DIM. Rumination time (RT) was monitored by sensors. Within parity, cows were ranked based on average...
Our objectives were to evaluate the effects of complete replacement of inorganic salts of trace minerals (STM) with organic trace minerals (OTM) in both pre- and postpartum diets on ovarian dynamics, estrous behavior measured by sensors, preimplantation conceptus development, and reproductive performance in dairy cows. Pregnant cows and heifers (n...
Our objectives were to evaluate the impact of supplementary trace mineral (TM) form-inorganic salts (STM; Co, Cu, Mn, Zn sulfates, and Na selenite) or organic (OTM; Co, Cu, Mn, Zn proteinates, and selenized yeast)-in the prepartum diet on quantity and quality of colostrum, passive immunity, antioxidant biomarkers, cytokine responses to lipopolysacc...
Our objectives were to evaluate the effects of complete replacement of supplementary inorganic salts of trace minerals (ITM; Co, Cu, Mn, Zn sulfates and Na selenite) by organic trace minerals (OTM; Co, Cu, Mn, Zn proteinates and selenized yeast) in both pre- and postpartum diets on TM concentrations in body fluids and liver, antioxidant and inflamm...
Our objectives were to evaluate the effect of the form of supplementary trace minerals—inorganic salts (STM: Co, Cu, Mn, and Zn sulfates and Na selenite) or organic (OTM: Co, Cu, Mn, Zn proteinates, and selenized yeast)—fed at 100% of recommended levels in both pre- and postpartum diets on in vitro phagocytic activity of neutrophils, and in vivo Ig...
The objective of this study was to investigate the effects of adding β-mercaptoethanol (βME) to culture medium of bovine in vitro -produced (IVP) embryos prior to or after vitrification on embryo development and cryotolerance. In Experiment I, Day-7 IVP blastocysts were vitrified and, after warming, cultured in medium containing 0, 50 or 100 μM βME...
The objective of this systematic review and meta-analysis was to identify the benefits and possible adverse side effects of oxytocin use during farrowing. Randomized controlled trials that were published in English within the last 50 years were eligible for inclusion. Eligible research needed to contain the PICO elements: population (P)-sows at far...
Our objectives were to evaluate the effects of complete replacement of supplementary inorganic salts of trace minerals (STM) by organic trace minerals (OTM) in both pre- and postpartum diets on feeding behavior, ruminal fermentation, rumination activity, energy metabolism, and lactation performance in dairy cows. Pregnant cows and heifers (n = 273)...
Our objectives were to identify factors associated with changes in prepartum DMI, and to evaluate its consequence to transition metabolism and performance. Cows (n = 273) were assigned to automatic feeding bins to measure DMI from 45 d prepartum to 98 DIM. Within parity and season, cows were ranked based on relative change in DMI from period 1 (d −...
This study's objectives were to evaluate the association of plasma concentration of anti-Mullerian (AMH) hormone at the first GnRH (G1) of breeding OvSynch in lactating Holstein cows with a genomic prediction of daughter pregnancy rate (GDPR) and ovulation response. We also evaluate the relationship of AMH at days -10, -8, -3, and 19 from timed AI...
The objective was to characterize transcriptome changes associated with elongation in bovine conceptuses and endometrium during preimplantation stages. Non-lactating Holstein cows (n = 48) enrolled in an experiment aimed at evaluating the effects of follicular wave and progesterone concentration during ovulatory follicle growth were used. Cows were...
Objectives were to evaluate the effects of follicular wave and progesterone concentration on growth of the ovulatory follicle, conceptus elongation, uterine IFN-τ concentration, and transcriptome of conceptus and endometrium of pregnant cows on d 17 of gestation. Nonlactating nonpregnant Holstein cows were assigned randomly to one of 3 treatments:...
The objective of this experiment was to determine how source of selenium (Se) affects dairy cow performance, antioxidant status, and apparent absorption and retention. Multiparous Holstein cows (n = 24; 597 ± 49 kg BW) were blocked by days in milk (161 ± 18) and randomly assigned to receive 0.3 mg/kg of either: 1) organic Se (selenized yeast; ORG;...
Our objective was to investigate the lipid content of uterus, blood plasma, and milk at early, mid, and late diestrus. Lactating cows (n = 30) had the estrous cycle and ovulation synchronized by administration of exogenous hormones. Cows were blocked by parity and assigned randomly to receive transcervical uterine flushing and biopsy on d 5 (early...
Pregnancy failure in dairy cattle appears to be attributable to losses during embryogenesis, mainly within the first month. Models have estimated that pregnancies lost after day 30 of fertilization cost producers US $550/cow. The reasons for these losses are not well understood, and the transcriptome profile of the reproductive tract during the est...
The concept of metabolic zonation in the liver proposes that opposing or complementary metabolic pathways are carried out within distinct regions of the liver lobule to maintain optimal metabolic homeostasis. As bovine placentomes differ in mass and shapes within the reproductive tract, which is even more pronounced in cloned-derived concepti, the...
Systemic inflammation (SI) is increasingly studied in several species because it may be central in many metabolic disturbances and be a risk factor for clinical disease. This proof-of-concept study evaluated the effects of the anti-inflammatory drug meloxicam on markers of SI and energy metabolism, polymorphonuclear neutrophil (PMN) function, and e...
The objective of this study was to investigate whether health, survival, and performance of dairy heifers from birth through first lactation are associated with parity and health status of their dams. Holstein heifers (n = 1,811) derived from artificial insemination were categorized as (1) daughters of primiparous cows that, consequently, were nonl...
Two retrospective studies examining data of 7,500 lactating cows from a single herd were performed with the objective of evaluating the long-term effects of clinical disease during the early postpartum period on milk production, reproduction, and culling of dairy cows through 305 days in milk (DIM). In the first study, data regarding health, milk p...
Most dairy cows experience a transient decrease in feed intake in the 1 to 2 wk before calving, which has been associated with systemic inflammation (SI), indicated by increased blood haptoglobin (Hp) concentration. We aimed to characterize the association between prepartum decrease in feed intake and the onset of SI and, if present, the ability of...
Elongation of the preimplantation conceptus is a
requirement for pregnancy success in ruminants, and
failures in this process are highly associated with subfertility
in dairy cattle. Identifying genetic markers
that are related to early conceptus development and
survival and utilizing these markers in selective breeding
can improve the reproductive...
Abstract
The objectives were the determine the effects of presynchronization with PGF2α 2 days before the 5-d timed artificial insemination (AI) protocol on ovarian responses and pregnancy per AI (P/AI) in dairy heifers. The hypothesis was that PGF2α would induce responsive heifers to be in proestrus at the initiation of the timed AI protocol, whic...
This study (1) investigated the effect of dietary transition at dry off on the behavior and physiology of Holstein dairy cows and (2) evaluated the efficacy of utilizing reductions in dietary nutrient density and milking frequency to mediate the dry-off process. Forty-eight cows were dried off over a 5-d period approximately 60 d before their expec...
Our objectives were to characterize parameters of luteal activity based on milk progesterone concentration (P4c) data from before and after artificial insemination (AI) and to evaluate their potential association with fertility in Holstein cows. Records of AI events (n = 4,353) and of milk P4c (n = 158,961) obtained through an in-line milk analysis...
Our objective was to investigate the lipid content of the uterus and milk at early-, mid-, and late-diestrus. Lactating cows (n = 30) had the estrous cycle and ovulation synchronized by administration of exogenous hormones. Cows were blocked by parity and assigned randomly to receive transcervical uterine flushing and biopsy on day 5 (early-diestru...
Nuclear reprogramming in somatic cell cloning is one of the key factors for proper development, with variations in the protocol appearing to improve cloning efficiency. This study aimed to determine the effects of two fusion-activation intervals and the aggregation of bovine cloned embryos on subsequent in vitro and in vivo development. Zygotes pro...
Objectives were to evaluate the effects of altering the dietary ratio of omega-6 (n-6) to omega-3 (n-3) fatty acids on the profile of fatty acids and expression of genes related to the prostaglandin biosynthesis on endometrial tissue, uterine secretion of PGF2α, and timing of spontaneous luteolysis in dairy cows. Multiparous lactating Holstein cows...
Elongation of the preimplantation conceptus is a prerequisite for maternal recognition of pregnancy and implantation in ruminants. Failures in this phase of development likely contribute for the subfertility of lactating dairy cows. This review will discuss our current understanding of the physiological and cellular requirements for successful elon...
The objective of this study was to evaluate the impact of clinical diseases in the early postpartum on culling, production and reproduction of dairy cows up to 305 d in milk (DIM). Detailed data regarding health, production, reproduction, and culling of 5,085 cows that calved in 2012 in a single herd were summarized. Of all cows, 1,534 cows had at...
Pregnancy losses are substantial in dairy cattle and threaten reproductive efficiency. A substantial proportion of these losses occur during early stages of conceptus development, including the elongation phase. Elongation of the preimplantation conceptus is a prerequisite for maternal recognition, implantation, and survival of pregnancy. The onset...
Inflammatory diseases postpartum have long-lasting effects on reproduction of dairy cows and increase substantially the likelihood of pregnancy losses. The objective of this study was to investigate whether the lasting effects of inflammatory diseases extends into postnatal life in pregnancies that survive until term. Incidence of diseases (metriti...
The aim of this study was to determine the effects of the cumulative gain in expertise in carrying out handmade cloning (HMC) procedures on embryo yield and pregnancy outcome in cattle. Results from in vitro and in vivo embryo development after HMC during three periods of 7 months, separated by 3-month intervals, were compiled and designated as P1,...
Inflammatory diseases are prevalent in cattle and impair fertility. Cows affected by inflammatory disease from parturition to the day before breeding have reduced fertilization of oocytes, reduced survival of zygotes to the morula stage, impaired development to early stages of conceptus elongation, reduced secretion of interferon during the period...
Reproductive inefficiency compromises the profitability of dairy herds and the health and longevity of individual cows. In the average dairy herd, the combination of estrus detection and ovulation synchronization protocols yields the best economic return. Genomic selection of animals is particularly profitable in situations in which little is known...
Elongation of the preimplantation conceptus is a prerequisite for successful pregnancy in ruminants and depends on histotroph secretion by the endometrium. Lipids are an essential component of the histotroph and recent studies indicate lipids have important roles in the elongation phase of conceptus development. The onset of elongation is marked by...
Resumption of ovulation after parturition is a coordinated process that involves recoupling of the GH/insulin-like growth factor 1 axis in the liver, increase in follicular development and steroidogenesis, and removal of negative feedback from estradiol in the hypothalamus. Infectious diseases and metabolic disorders associated with extensive negat...
Cloning procedures often interfere with conceptus growth and life ex utero, in a set of symptoms known as abnormal offspring syndrome (AOS). The aim of the present study was to compare the developmental pattern of in vivo-derived (IVD), IVF-derived and handmade cloning-derived (NT-HMC) Day 225 bovine concepti using established procedures. Pregnancy...
The objectives were to characterize changes in transcriptome of preimplantation conceptuses at the onset of elongation and associated changes in uterine histotroph composition and endometrial physiology. Lactating dairy cows (n=160) had ovulation synchronized for artificial insemination (AI; study Day 0). On Day 15, uteri were flushed and endometri...
The objectives were to compare development and transcriptome of preimplantation conceptuses 15 d after synchronized ovulation and artificial insemination (AI) according to the genetic background of the cow and estrous cyclicity at the initiation of the synchronization program. On d 39 ± 3 postpartum, Holstein cows that were anovular (HA; n = 10), H...
Pregnancy-associated glycoproteins (PAG) are produced by the ruminant placenta and secreted into the maternal circulation throughout pregnancy. The extent to which circulating PAG concentrations predict pregnancy outcomes was examined herein. Also, associations between circulating PAG concentrations and various production parameters and clinical di...