Eduardo Fuentes

WriteWise Inc.

Academic writing is one of the most valuable skills a scientist can develop. A primary challenge for graduate students is to coherently and concisely organize and present ideas within a manuscript. Writing a quality research manuscript requires transmitting the most relevant information through precise sentences that fulfill diverse communicational...

In this work was shown how k − shell decomposition helps to understand the dynamics of the formation of the decentralized and collaborative language community defined by the electronic repository arXiv. Our results suggest that there are several global patterns that emerges from the microscopic activity of users sharing content. The growth of the c...

WriteWise represents the first commercially available advanced platform that provides user's help and feedback to improve scientific papers writing. This is thanks to the development of and advance textual data representation at different linguistic levels (e.g. words, sentences) through using cutting-edge machine-learning models and applied lingui...

We demonstrated the effectiveness of both the USE and BioSentVec as methods for helping users identify and improve semantic similarity between sentences in bio-medical texts. The shared tendencies between the models support sequential similarity as a metric to evaluate a text’s cohesion. With both methods outliers can be easily spotted, and then sp...

One of the main challenges for researchers when writing scientific papers is to coherently structure and organize the content, specifically at rhetorical-discursive level. Modeling these types of text is difficult and new computation approaches are necessary. Currently, language model pre-training that learned word representations from a large amou...

[This corrects the article DOI: 10.1371/journal.pone.0044256.].

Much attention has been given to insulin-like growth factor (Igf) pathways that regulate the balance of skeletal muscle protein synthesis and breakdown in response to a range of extrinsic and intrinsic signals. However, we have a less complete understanding of how the same signals modulate muscle mass upstream of such signalling, through a family o...

Muscle-specific RING finger (MuRF) proteins are E3-ubiquitin ligases and key regulators of muscle growth and turnover. Here, using a range of phylogenomic approaches, we generated a comprehensive list of members of the vertebrate MuRF family. Adding to the previously-reported MuRF1, MuRF2 and MuRF3, we identified a novel family member, MuRF4, which...

One of the most fundamental biological processes in living organisms that is affected by environmental fluctuations is growth. In fish, skeletal muscle accounts for the largest proportion of body mass, and the growth of this tissue is mainly controlled by the Insulin-like Growth Factor (IGF) system. By using the carp (Cyprinus carpio), a fish that...

Myostatin (MSTN) is the main negative regulator of muscle growth and development in vertebrates. In fish, little is known about the molecular mechanisms behind how MSTN inactivation triggers skeletal muscle enhancement, particularly regarding the signaling pathways involved in this process. Moreover, there have not been reports on the biotechnologi...

Skeletal muscle differentiation is a complex and highly regulated process characterized by cell cycle arrest, which is associated with morphological changes including myoblast alignment, elongation, and fusion into multinucleated myotubes. This is a balanced process dynamically coordinated by positive and negative signals such as the insulin-like g...

The growth hormone (GH)-insulin-like growth factor (IGF) system is the key promoter of growth in vertebrates; however, how this system modulates muscle mass in fish is just recently becoming elucidated. In fish, the GH induces muscle growth by modulating the expression several genes belonging to the myostatin (MSTN), atrophy, GH, and IGF systems as...

Insight of how growth and metabolism in skeletal muscle are related is still lacking in early vertebrates. In this context, molecules involved in these processes, such as leptin, AMP-activated protein kinase (AMPK), target of rapamicyn (TOR), peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α, and oxidative phosphorylation complexe...

In the present study, different reference genes were isolated, and their stability in the skeletal muscle of fine flounder subjected to different nutritional states was assessed using geNorm and NormFinder. The combinations between 18S and ActB; Fau and 18S; and Fau and Tubb were chosen as the most stable gene combinations in feeding, long-term fas...

The IGF-binding proteins (IGFBPs) play a dual role in the regulation of the activity and bioavailability of IGFs in different tissues. Diverse evidence has shown that IGFBPs can inhibit and/or potentiate IGF actions. In this study, igfbp1, 2, 3, 4, 5, and 6 were isolated in the fine flounder, a flat fish species that shows slow growth and inherent...

A detailed understanding of how the GH and IGF-I regulate muscle growth, especially in early vertebrates, is still lacking. The fine flounder is a flatfish species exhibiting remarkably slow growth, representing an intriguing model for elucidating growth regulatory mechanisms. Key components of the GH system were examined in groups of fish during p...

The insulin-like growth factor-I (IGF-I) is a key regulator of skeletal muscle growth in vertebrates, promoting mitogenic and anabolic effects through the activation of the MAPK/ERK and the PI3K/Akt signaling pathways. Nutrition also affects skeletal muscle growth, activating intracellular pathways and inducing protein synthesis and accretion. Thus...

Insulin-like growth factor-1 and insulin-like growth factor-1 receptor (IGF-1 and IGF-1R) play main roles in vertebrate growth and development. In fish, besides contributing to somatic growth, both molecules exhibit pleiotropic functions. We isolated complete cDNAs sequences encoding for both IGF-1 and IGF-1R in the Chilean flounder by using RT-PCR...

The full length cDNA sequence of the myostatin gene was cloned from a teleostean fish, the Chilean flounder (Paralichthys adspersus) through RT-PCR amplification coupled with the RACE approach to complete the 5'- and 3'-region. The deduced amino acid sequence encodes a protein of 377 amino acid residues, including the structural domains responsible...

The entire cDNA sequence of the growth hormone receptor (GHR) of the Chilean flounder (Paralichthys. adspersus) was cloned by RT-PCR and RNA ligase rapid amplification of 5' and 3'ends. The deduced amino acid sequence contains 641 residues and codes for the GHR1 form. The receptor includes all the structural domains and motifs responsible for its i...