Eduardo Freitas

• Federal University of Minas Gerais

Pastures are the basis of ruminant feed; however, forage digestibility decreases in the dry season. In this study, the aims were to identify selected fungus isolates from the gastrointestinal tract of healthy Santa Inês sheep and to evaluate the in vitro digestibility of Urochloa decumbens (UD) hay inoculated with these fungi. Sequence analysis of...

The aim of this study was to evaluate the cellulase and xylanase activity of fungi isolated from the digestive tract of sheep and the effects of supplementation with two selected fungi on intake, digestibility, microbial protein (MP) synthesis and nitrogen balance in lambs. The first experiment evaluated the carboxymethylcellulase (CMCase) and xyla...

Commercial yeast has been frequently employed to improve the digestibility of ruminant diets, albeit these microorganisms are not indigenous to the rumen microbiome. In this investigation, our aim was to assess the in vitro digestibility of Urochloa decumbens hay (UDH) with the incorporation of two selected yeast strains isolated of ruminal fluid o...

O ambiente ruminal é composto por inúmeros microrganismos, onde cada um deles possui um papel fundamental no aproveitamento das dietas ingeridas pelos ruminantes, os fungos são seres unicelurares que atuam degradando as porções fibrosas dessa dieta. Objetivou-se neste estudo avaliar a atividade celulolítica (AC) de fungos micelianos aeróbios proven...

The addition of cellulolytic fungi, or their enzymes, in diets containing high levels of fiber are promising strategy for improving the performance. In this study, the aims were to select cellulolytic fungi from the digestive tract of sheep fed different concentrations of banana leaf hay. Thirty lambs raised in a feedlot were evaluated, distributed...

Objetivou-se avaliar o desempenho da adição de uma cepa de levedura isolada do líquido ruminal de ovino hígido na dieta de cordeiros desmamados e alimentados com feno de Urochloa decumbens. No estudo, foram utilizados doze cordeiros, Santa Inês x Dorper, recém-desmamados. Os animais passaram por um período de adaptação referente às instalações (15...

Degradation of lignocellulosic materials by Aspergillus terreus isolated from rumen fluid, fungal biomass, in vitro substrate degradation, pH at end of incubation, concentrations of total protein (TP) and total cellulase (TC) were performed.In prior assay, submerged fermentations of sugarcane bagasse (SB) or Urochloa decumbens (UD) by five A.terreu...

Fungi of the genus Trichoderma hydrolyze lignocellulosic materials, thus promoting the production of fermentable sugars. In the present study, the production of cellulases by Trichoderma longibrachiatum from ruminal fluid was evaluated in submerged fermentation of Urochloa decumbens hay (UD) or sugarcane bagasse (SB). Moreover, the biomass of this...

In this study, we evaluated the productive performance and economic profitably of weaned lambs fed low quality hay and supplemented with a Trichoderma longibrachiatum strain isolated from sheep (ICA/UFMG LT 001). Twelve early-weaned Santa Inês x Dooper lambs were housed in individual pens during 63 days, and randomly split into two groups: (1) lamb...

The objective with this study was to evaluate intake, feeding behavior, rumen fluid characteristics, performance, and nutrient digestibility of sheep fed banana pseudostem hay (BPH) with and without virginiamycin (VM). Thirty-two uncastrated male crossbred Dorper × Santa Inês lambs at approximately five months of age, with an average initial body w...

Background Commercial yeast strains are a promising alternative to chemical additives in ruminants; but are not self to the rumen ecosystem. In this study, the aim was to identify and evaluate the growth, mass production, and enzymatic activity of yeast strains naturally isolated from bovine rumen in submerged fermentations containing roughages wit...

The authors have withdrawn this preprint due to a need to make corrections.

The objective of this study was to evaluate carcass yield, measurements, cuts, and fat deposition of sheep fed banana pseudostem hay and Tifton 85 grass hay, with or without virginiamycin. Thirty-three uncastrated male Dorper × Santa Ines crossbred lambs at five months of age, with an average body weight of 25.00 ± 1.95 kg, were used in a feedlot e...

The objective of this review was to survey the main microbial species producing enzymes involved in the degradation of compounds such as cellulose, hemicellulose and lignin. Most of the microorganisms isolated from soil, waste and compost material are capable of producing a broad spectrum of degrading enzymes from the plant cell wall. This enzymati...

A ciliate protozoa suppression can reduce methane production increasing the energy efficiency utilization by ruminants. The physicochemical characteristics of rumen fluid and the profile of the rumen protozoa populations were evaluated for sheep fed banana leaf hay in replacement of the Cynodon dactylon cv. vaqueiro hay. A total of 30 male sheep we...

The objective of this study was to evaluate the aerobic rumen mycobiota from three age groups of Nelore beef cattle reared extensively on lignified pasture. The experiment was randomized and sampled 50 steers, 50 cows, and 50 calves grazed on Brachiaria spp. pasture during the dry season. Rumen fluid in all animals was aromatic, slightly viscous, a...

Protozoa of the rumen can represent 40% of total nitrogen and 60% of the final product of fermentation and it has cellulolytic activity. This study aimed to quantify and identify the ruminal protozoa populations from steers, raised exclusively on pastures during the dry season in the north of Minas Gerais, Brazil. Samples of ruminal fluid of 36 Nel...

The objective of this study was to characterize the population of Enterobacteriaceae in the rumen of steers raised in tropical pastures and steers fed no roughage. Were evaluated 70 crossbred Nellore cattle, 50 steers raised on extensive grazing Brachiaria spp. and 20 calves reared in confinement for 71 days, receiving only concentrated. Samples of...

avaliados 49 isolados, sendo 27 Aspergillus spp., quatro Gliocladium spp., seis Paecilomyces spp., nove Rhizophus spp. e três Scedos-porium. Esses isolados foram incubados em meio C, contendo celulose microcristalina a 1% como única fonte de carbono, e incubados em estufa a 37°C em triplicata. Os índices de atividades celulolíticas foram verificado...

The objective was to evaluate the cellulolytic activity of aerobic mycelian fungi from dairy cattle fed tropical forages. Isolates of fungi were obtained from 30 samples of rumen fluid from cows fed sorghum silage, 32 cows fed on Brachiaria brizantha, 12 heifers receiving sorghum silage and 11 heifers fed cane sugar. The isolation was performed on...

Anaerobic ruminal fungi are important for digestion of lignified plant fibers. This work aimed to evaluate the detection rate of these fungi in ruminal fluid of calves and dairy cows fed with different sources of tropical forages. Holstein animals were sampled, 30 cows fed sorghum silage, 30 cows fed on Brachiaria brizantha pasture, 12 calves suppl...

The objective of this study was to evaluate the aerobic microbiota of the rumen fluid from Holstein cows and heifers fed different tropical forage in the north of Minas Gerais, Brazil. A total of 30 samples of rumen fluid from cows fed with sorghum silage were collected: 32 from cows fed Brachiaria brizantha, 12 from heifers that received sorghum s...

Fungi populations were evaluated in large intestine from sheep raised in the North of Minas Gerais, Brazil. The samples were from 39 Santa Inês crossbred ewes and 30 lambs raised on Tanzania grass (Panicum maximum cv. Tanzania), both supplemented with mineral mixtures. The clinical specimens were directly collected from rectal ampoule with sterile...

Potencialidade e limitações da utilização de aditivos na produção de bovinos de corte crescimento. Nas últimas décadas intensificaram-se os esforços para melhorar esses índices (GOES, 2004), e com isso, a adoção de tecnologias torna-se essencial. Dentro das possibilidades tecnológicas disponíveis para o produtor, o aditivo vem como uma ferramenta q...

This study aimed to evaluate the effect of sewage sludge and calcium and magnesium silicate on the physical attributes of a Cambisol cultivated with sunflower. The treatments were arranged in a factorial (4 x 2) + 1, corresponding to four doses of sewage sludge (0, 6.25, 12.5 and 18.5 t ha-1, in dry basis), two doses of calcium and magnesium silica...

Yeasts suplemented in the rumen have been produced benefic interations in the digestion and in the health of the ruminants. This study aimed to quantify, to isolate and, to identify aerobic fungi and yeasts naturally present in the rumen of goats and cattle raised on tropical pastures of the North of Minas Gerais State, Brazil. Samples of 15mL of r...

Atividade celulolítica de fungos micelianos aeróbios provenientes de bovinos leiteiros alimentados com diferentes tipos de volumosos tropicais suplementação mineral. A quantificação, isolamento e identificação dos isolados foram realizadas em meio Celulose ou em meio Sabouraud. Avaliou-se a atividade celulolítica de 49 amostras de fungos micelianos...

Objetivou-se com o presente trabalho avaliar as características físico-químicas e microbiológicas das silagens ácidas de pescado preparadas a partir de peixes inteiros. Foram analisadas duas silagens. A primeira foi constituída de ácido acético (5% da biomassa) (S1) e a segunda de ácido lático (5% da biomassa) (S2), armazenadas em tubos de ensaio a...

Resumo: O rúmen alberga várias espécies de microrganismos pertencentes a diferentes reinos. Vários fatores que interferem na fisiologia do ruminante podem influenciar significativamente a microbiota do rúmen. O presente trabalho teve como objetivo avaliar o suco do rúmen e quantificar, isolar e identificar a população de fungos e enterobacteriacea...

Resumo A ensilagem de forragem é um processo de conservação que tem como objetivo final reduzir as perdas do valor nutricional e permitir o suprimento de alimentos de qualidade durante os períodos de seca. Poucos estudos têm avaliado a ocorrência de leveduras naturalmente encontradas em silagem de forrageiras como o milho, sorgo ou capim. O present...