Eduardo Cortón

Eduardo Cortón
Universidad de Buenos Aires | UBA · Department of Biological Chemistry

PhD

About

72
Publications
15,918
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1,082
Citations
Citations since 2016
28 Research Items
666 Citations
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120

Publications

Publications (72)
Preprint
Co-exposure soil studies of pollutants are necessary for an appropriate ecological risk assessment. Here, we examined the effects of two-component mixtures of metal oxide nanoparticles (NPs, ZnO or goethite) with chlorpyrifos (CPF) under laboratory conditions in short-term artificial soil assays using Eisenia andrei earthworms. We evaluated effects...
Article
Microbial fuel cells (MFCs) receive substantial research interest for their eco-friendly and energy conversion ability. Along with the production of electrical energy, a synergistic benefit of MFCs is also gained from their capability to reduce organic contaminant load in polluted water. The sub-categories of MFCs such as Sediment MFC and Plant MFC...
Article
Full-text available
Rapid diagnosis by using small, simple, and portable devices could represent one of the best strategies to limit the damage and contain the spread of viral, bacterial or protozoa diseases, principally when they can be transmitted by air and are highly contagious, as some respiratory viruses are. The presence of antibodies in blood or serum samples...
Article
Full-text available
A novel assay technique that involves quantification of lysozyme (Lys) through machine learning is put forward here. This article reports the tendency of the well- documented Ellington group anti-Lys aptamer, to produce aggregates when exposed to Lys. This property of apta-aggregation has been exploited here to develop an assay that quantifies the...
Article
Hydrogen production was evaluated in two-chamber microbial electrolysis cells (MEC), where the chambers of the cell were separated using a new economical and environmentally friendly membrane made of poly (vinyl) alcohol/chitosan (PVA/CS). The MEC performance was compared to that of Nafion. The obtained results indicated that the MEC performance fo...
Article
Bacteria-mineral interaction is important because it defines key steps in the formation of a biofilm, which is a fundamental structure in the attack on minerals. Bacterial cell attachment that results in biofilm formation is the first step of bacteria-mineral interaction, and it is known that they strongly depend on the chemical characteristics of...
Article
Three of the primary bottlenecks, which should be consider for practical, point-of-need use of microbial fuel cell (MFC) analytical devices were surpassed in this work: i) the use of a diffusive barrier, hence, an electrogenic biofilm; ii) longer enrichment/stabilization times to produce a biofilm, made in a laboratory environment, over the electro...
Article
Full-text available
Microbial fuel cells (MFCs) can evolve in a viable technology if environmentally sound materials are developed and became available at low cost for these devices. This is especially important not only for the designing of large wastewater treatment systems, but also for the fabrication of low-cost, single-use devices. In this work we synthesized me...
Article
This study describes for the first time the development of 3D printed microfluidic devices with integrated electrodes for label-free counting of E. coli cells incorporated inside droplets based on capacitively coupled contactless conductivity detection (C ⁴ D). Microfluidic devices were fully fabricated by 3D printing in the T-junction shape contai...
Article
In biohydrometallurgical processes, a mineral exhibits different oxidation phases due to the system's heterogeneity, especially in heap leach pads. Oxidation chemically modifies the mineral surface altering its interface, and thereby affecting the bacteria-mineral interaction, mineral reactivity and leaching velocity. Given that the mineral can be...
Conference Paper
Microbial fuel cells (MFCs) and microbial electrolysis cells (MECs) are well known bioelectrochemical systems (BESs) that can be used to produce electricity, and hydrogenrespectively, by means of microbial metabolic activity. Nonetheless, new strategies forconstructions, start up, operation and maintenance are required to improve BESs efficiency an...
Article
Full-text available
Size, shape and surface characteristics strongly affect interfacial interactions, as the presented among iron oxide nanoparticles (NPs) aqueous colloids and bacteria. In other to find the forces among this interaction, we compare three types of surface modified NPs (exposing oxalate, arginine or cysteine residues), based on a simple synthesis and d...
Preprint
Full-text available
One limiting aspect to make microbial fuel cells (MFCs) a viable technology is to obtain low cost and environmentally sound materials for their components. In this work we synthesized membranes by a simple procedure involving low price and biodegradable materials such as poly (vinyl alcohol) (PVA), chitosan (CS) and PVA:CS, all cross-linked with su...
Article
Glassy carbon electrodes (GC) were modified with multiwalled carbon nanotubes (MWCNT/GC) and electrochemically treated first by applying an oxidation potential and then a reduction potential. The resulting electrodes were characterized via scanning electron microscopy, Raman spectroscopy, energy dispersive spectroscopy, and electrochemical techniqu...
Article
The present study was aimed to test an electrochemical sensing approach for the detection of an active chemolithotrophic metabolism (and therefore the presence of chemolithotrophic microorganisms) by using the corrosion of pyrite by Acidithiobacillus ferrooxidans as a model. Different electrochemical techniques were combined with adhesion studies a...
Article
Full-text available
En nuestro laboratorio nos dedicamos desde hace más de 10 años a la investigación, docencia y extensión. El objetivo del Laboratorio de Biosensores y Bioanálisis es el desarrollo de métodos analíticos que permitan la cuantificación de diferentes parámetros relacionados con el medio ambiente, la salud y la industria. En este artículo presentamos nue...
Article
Chalcopyrite is the main ore mineral used in industrial copper extraction. However, when passivation processes occur during hydrometallurgical treatment, a large percentage of the mineral being treated is not solubilized, so the copper recovery is limited. Galvanic interactions between semiconductor minerals are the basis of many strategies to achi...
Article
Modification of cellulosic paper with carbon nanotubes (CNT) was studied for the development of electronic and analytical devices. Interesting results were published by using a CNT aqueous solution and the capillary forces of filter paper to make conductive tracks, supercapacitors, potentiometric electrodes and chemometric sensors. In this report,...
Conference Paper
Full-text available
Presentamos una propuesta de diseño experimental de Celdas de Combustible Microbianas que puede ser adaptado al formato escolar y las opiniones de docentes en servicio en distintos niveles educativos y estudiantes de profesorado de Química -nivel terciario-, acerca de la posibilidad de trabajar con un material innovador que aborde la generación de...
Article
The aim of this work was to use electrochemical methods, capable to follow the corrosion of minerals, in order to monitor the progressive attack of the bacteria on the mineral. The assay was performed in a three electrode cell, with pyrite as the working electrode. The tests were performed in the absence and presence of iron; when present it was in...
Article
Full-text available
Electrogenic bacteria are organisms that can transfer electrons to extracellular electron acceptors and have the potential to be used in devices such as bioelectrochemical systems (BES). In this study, Dietzia sp. RNV-4 bacterium has been isolated and identified based on its biochemical, physiological and morphological characteristics, as well as b...
Data
(Figure A) Absorption spectra of the ethanol fraction obtained from the supernatants, free of cells, of the BES. The full line represents commercial canthaxanthin extract and the dashed line represents the extract of the Dietzia sp. RNV-4 culture supernatant. (Figure B). Response of anodic current peak of cell-free supernatant to the canthaxanthin...
Chapter
This chapter presents a survey of developments and bioassay applications of individually addressable electrochemical arrays covering the past two decades and including over 90 references. Many different array designs have been fabricated, from macroscopic devices that have two to four sensing elements to ultramicroelectrode arrays containing as man...
Article
Spheres, tubes and planar shaped nanomaterials as Fe3O4 nanoparticles (MNPs), multi walled carbon nanotubes (MWCNT) and graphene oxide (GO) were used for the first time to treat microfluidic paper−based analytical devices (μPADs) and create a biocompatible layer with high catalytic surface. Once glucose measurements are critical for diabetes or gly...
Article
Microbial fuel cells (MFCs) are bioelectrochemical systems (BES) capable of harvesting electrons from redox reactions involved in metabolism. In a previous work, we used chemoorganoheterotrophic microorganisms from the three domains of life-Bacteria, Archaea, and Eukarya-to demonstrate that these BES could be applied to the in situ detection of ext...
Article
Iron ions are monitored in processes such as bio-leaching, bio-oxidation, ferric leaching, passivation control, and others. The role of iron in different hydrometallurgy processes is very important because it affects strongly several industrial ore production, as chalcopyrite cooper extraction. In this work we present an amperometric FIA system tha...
Article
Here, we present a new toxicity bioassay (CO2-TOX), able to detect toxic or inhibitory compounds in water samples, based on the quantification of Pseudomonas putida KT2440 CO2 production. The metabolically produced CO2 was measured continuously and directly in the liquid assay media, with a potentiometric gas electrode. The optimization studies wer...
Article
Microinterdigitated electrode structures (μIDES) have widely been used for electrochemical cell impedance spectroscopy (ECIS). For instance ECIS has been used to study biofilm formation and growth of a wide range of microorganisms to detect bacterial infections, food contaminations, biocorrosion, and biofouling. In this study we describe the develo...
Article
Based on the classical Gram staining technique we created an adapted protocol so it could be used in bacterial suspensions for Gram positive and negative bacteria. The differences between these two types are determined by spectrophotometry. This is a rapid and low cost procedure to classify bacteria.
Article
Full-text available
Se denomina biohidrometalurgía al tratamiento de recuperación de metales de interés económico a partir de minerales sulfurados, utilizando microorganismos. Es una tecnología que ha cobrado mayor importancia en las últimas décadas, tanto desde el punto de vista científico como tecnológico por sus aplicaciones actuales, y mayores oportunidades futura...
Article
Full-text available
Las celdas de combustible biológicas son dispositivos bio-electroquímicos, donde generalmente el ánodo (pero a veces el cátodo, o ambos) contiene microorganismos capaces de generar y mantener un gradiente electroquímico que se utiliza típicamente para generar energía eléctrica. En la configuración más estudiada, el ánodo contiene bacterias heterótr...
Conference Paper
Full-text available
The detection of pathogenic bacteria is key for the identification and prevention and of problems related to health and safety. Accurate and quick detection and quantification of pathogen microorganisms in food, drinking water and in clinical diagnostics using cost-effective methodologies are greatest challenges currently facing food industry, heal...
Conference Paper
In the bio-mining industry there are various compounds which need to be frequently monitored, one of which is iron, since its concentration can seriously affect the industrial process. This study describes the development of an electrochemical method of speciation and rapid quantification of iron in bio-mining processes conditions. Speciation and q...
Article
Abstract Microbial fuel cells were rediscovered twenty years ago and now are a very active research area. The reasons behind this new activity are the relatively recent discovery of electrogenic or electroactive bacteria and the vision of two important practical applications, as wastewater treatment coupled with clean energy production and power...
Article
Full-text available
Abstract Microbial fuel cells (MFCs) are bio-electrochemical devices, where usually the anode (but sometimes the cathode, or both) contains microorganisms able to generate and sustain an electrochemical gradient which is used typically to generate electrical power. In the more studied set-up, the anode contains heterotrophic bacteria in anaerobi...
Article
Full-text available
Nitrogen compounds like urea and melamine are known to be commonly used for milk adulteration resulting in undesired intoxication; a well-known example is the Chinese episode occurred in 2008. The development of a rapid, reliable and economic test is of relevance in order to improve adulterated milk identification. Cyclic voltammetry studies using...
Article
Full-text available
In this work we present a device specifically designed to study microbial growth with several applications related to environmental microbiology and other areas of research as astrobiology. The Automated Measuring and Cultivation device (AMC-d) enables semi-continuous absorbance measurements directly during cultivation. It can measure simultaneousl...
Article
This paper presents data obtained using an indigenous microbial community contained in anaerobic sediments (mud) collected from the shore of the Río de La Plata River (South America). After the sedimentary microbial fuel cells were assembled the evolution of current and power vs. time was studied. Two types of commercially available graphite materi...
Article
Aims: The survival rate of freeze-dried cultures is not enough information for technological applications of micro-organisms. There could be serious metabolic/structural damage in the survivors, leading to a delay time that can jeopardize the design of a rapid biochemical oxygen demand (BOD) metabolic-based bioassay. Therefore, we will study the m...
Conference Paper
Full-text available
At present, dwarf M stars are being considered as potential hosts for habitable planets. However, an important fraction of these stars are flare stars, which among other kind of radiation, emit large amounts of UV radiation during flares, and it is unknown how this events can affect life, since biological systems are particularly vulnerable to UV....
Article
Full-text available
The haloarchaea Natrialba magadii and Haloferax volcanii, as well as the radiation-resistant bacterium Deinococcus radiodurans, were exposed to vacuum UV (VUV) radiation at the Brazilian Synchrotron Light Laboratory. Cell monolayers (containing 10(5) to 10(6) cells per sample) were prepared over polycarbonate filters and irradiated under high vacuu...
Article
Full-text available
In this work, two archaea microorganisms (Haloferax volcanii and Natrialba magadii) used as biocatalyst at a microbial fuel cell (MFC) anode were evaluated. Both archaea are able to grow at high salt concentrations. By increasing the media conductivity, the internal resistance was diminished, improving the MFC’s performance. Without any added redox...
Article
Full-text available
Since the 1970s, when the Viking spacecrafts carried out experiments to detect microbial metabolism on the surface of Mars, the search for nonspecific methods to detect life in situ has been one of the goals of astrobiology. It is usually required that a methodology detect life independently from its composition or form and that the chosen biologic...
Article
As aerobic respiration proceeds it consumes oxygen and produces carbon dioxide; and the relation between these two parameters, the respiratory quotient (RQ), is related to the type of substances being respired. Therefore, for a given water or wastewater origin, a more or less stable RQ is expected, making possible the estimation of BOD5 by means of...
Conference Paper
http://www.deetc.isel.ipl.pt/electronica/temp/ibersensor_proceedings__/ibersensor_proceedings/ibersensor_abstracts/ibersensor049.pdf
Article
Full-text available
Dwarf M stars comprise about 75 percent of all stars in the galaxy. For several years planets orbiting M stars have been discarded as suitable places for development of life. This paradigm now has changed and terrestrial-type planets within liquid-water habitable zones (LW-HZ) around M stars are reconsidered as possible hosts for life as we know it...
Article
Full-text available
Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose at different concentrations and temperatures. Whe...
Article
We describe a new, faster and convenient method to study some metabolic characteristics - by the successful application of immobilized yeast cells (S. cerevisiae) in a microbial biosensor-like device. Microbial biosensors consist of microorganisms immobilized on the surface of a membrane or in a gel, in close contact with a transducer. Almost all w...
Article
Molecularly imprinted polymers (MIPs) are easily obtained by a non-covalent approach through the copolymerisation of suitable functional monomers and cross-linkers in the presence of the print molecule. Removal of the template leaves a polymer that selectively recognises it. However, experimental data have demonstrated that non-covalent molecularly...
Article
Full-text available
Podemos definir biorremediación como la utilización de seres vivos para solucionar un problema ambiental, tales como suelo o agua subterránea contaminados. En un ambiente no contaminado, las bacterias, los hongos, los protistas, y otros microorganismos heterotróficos degradan constantemente la materia orgánica disponible, para obtener energía. Cuan...
Chapter
Half Title Title Copyright Contents Preface Acknowledgments
Chapter
Basic aspects and examples of biosensors, including definitions and applications, response of enzyme-based biosensors, examples of biosensor configurations, ferrocene-mediated amperometric glucose sensor, potentiometric biosensor for phenyl acetate, potentiometric immunosensor for digoxin, evanescent-wave fluorescence biosensor for bungarotoxin, op...
Article
Simple and understandable activities involving the manufacturing of hamburgers can be used as an analogy for stoichiometric concepts and calculations. Keywords (Audience): High School / Introductory Chemistry
Article
Rapid identification of Escherichia coli strains is an important diagnostic goal in applied medicine as well as the environmental and food sciences. This paper reports an electrochemical, screen-printed biosensor array, where selective recognition is accomplished using lectins that recognize and bind to cell-surface lipopolysaccharides and coulomet...
Article
How does one distinguish life from nonlife? This hands-on activity reproduces the life-test experiments carried out by the Viking Mars missions by simple nonradioactive laboratory experiments. A CO2 potentiometric electrode is used to measure the carbon dioxide released by the addition of glucose solution to soil and sand samples. The experiments i...
Article
Bacterial strains were isolated from contaminated waters, mud or soils. They are capable of growing in mineral medium with different chemicals as carbon source, such as aliphatic or aromatic hydrocarbons and polychlorinated biphenyls (PCBs). Most of these strains tolerate high concentrations (up to 30% v/v) of the xenobiotic substrates. This is par...
Article
The characterization of a biosensor based on poly(allylamine) containing an osmium complex (OsPAA) in a matrix of industrial relevance, like milk, is presented. The biosensor is constructed by crosslinking an oxidase and the redox polymer (OsPAA) with poly(ethylene diglycidyl) ether (PEG). Parameters such as the optimum crosslinker concentration, w...
Article
A novel electrochemical method for the recognition of different species of microorganisms has been developed. It consists of sampled DC voltammetry applied directly to microbial cultures or to the supernatants of these cultures obtained following centrifugation. Pt, Au, and glassy carbon working electrode materials were examined. Complete and repli...
Article
Four simple experiments are described, in two laboratory sessions of 3 hours each. They provide high school students of a combined science course with the opportunity to review and integrate many topics while participating in a hands-on activity that resembles real industrial problems. The first lab comprises the calibration of a CO2 potentiometric...
Article
A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (10(10) cfu mL(-)(1)) and particular pregrowth conditions were needed. Reduction of catabolite repression by monosaccharides was achieved by pre...
Article
This work presents a hands-on activity in which the principles of gaseous equilibria and electrochemical potentials are applied to the construction of a carbon dioxide detector. The device is constructed using a combination pH electrode and a minimal amount of inexpensive, normally available laboratory materials. The standardized sensor achieves a...
Article
A microbial sensor based on a carbon dioxide electrode coupled with immobilized Saccharomyces cerevisiae (baker's yeast) has been used for the determination of sucrose in dairy products. The sensor was used as the detector in a flow injection analysis system. Calibration curves for sucrose were established from 1 to 100 g/l. Determinations for...
Article
An Os hydrogel based on the covalent attachment of Os(byp)2 ClPyCHO to PAA-NH2 was cross-linked with glucose oxidase (GOx), lactate oxidase (LOx) and horseradish peroxidase (HRP) on GC and Au electrodes by PEG-400 bifunctional reagent. Single layer monoenzyme (GOx or LOx) and bienzyme (HRP-GOx) single layer modified electrodes were prepared with th...
Article
Two new redox-active osmium complexes of the type (Os(bpy)2Cl(pyX))+ have been synthesised, where pyX corresponds to a pyridine derivative bearing a functional group (aldehyde, carboxylate). Their electrochemical behaviour and electronic spectra were studied in acetonitrile and aqueous solutions. The rate coefficients for the re-oxidation of glucos...
Article
Full-text available
A ferricyanide-mediated short term biochemical oxygen demand (BOD st) approach has been reported to overcome low oxygen solubility in water limitation that contributes to long incubation time (5 days) in the standard BOD test (BOD 5), internationally employed as a water quality parameter. BOD test is also used to determine organic pollution in natu...

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