Edivaldo Ximenes Ferreira Filho

Edivaldo Ximenes Ferreira Filho
University of Brasília | UnB · Department of Cell Biology

Full Professor of Biochemistry
Professor of Bichemistry and Researcher at University of Brasília, Brazil.

About

132
Publications
29,004
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3,985
Citations
Citations since 2017
45 Research Items
2286 Citations
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Introduction
The researcher has experience in the field of ​​Biochemistry, with an emphasis on Enzymology, working mainly in the following lines of research: production, purification and biotechnological application of cellulases, hemicellulases and pectinases from filamentous fungi.

Publications

Publications (132)
Article
Full-text available
Lignocellulosic residues represent a low cost, high quality, and abundantly available alternative carbon sources that offer potential solutions to challenges linked to the growing demand for sustainability. In this study, the potential for the production of holocellulolytic enzymes induced by Paecilomyces formosus with agro-industrial coffee residu...
Article
Saccharification recalcitrance significantly limits the conversion of lignocellulosic biomass to valuable end products. The intricate arrangement between cell wall matrix polysaccharides, proteins, and lignin makes the cell wall structure a challenging substrate for carbohydrase and ligninase accessibility. Cellulose valorization from biomass or vi...
Article
Full-text available
L-asparaginase is an important enzyme in the pharmaceutical field used as treatment for acute lymphoblastic leukemia due to its ability to hydrolyze L-asparagine, an essential amino acid synthesized by normal cells, but not by neoplastic cells. Adverse effects of L-asparaginase formulations are associated with its glutaminase activity and bacterial...
Article
To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii. CAZymes domains were evaluated in the genome, and a screening of the enzymatic potential of A. tamarii in various...
Article
Full-text available
In this work, we have tested individual and combination of applications of ozonolysis and liquid hot water (LHW) to pretreat sugarcane bagasse (SCB) for the removal of enzyme and/or microbial inhibitors and generation of potential value-added chemicals. A solid content with 80% cellulose and a liquid phase (liquor) rich in phenolic derived compound...
Article
L-asparaginase é uma enzima importante no setor farmacêutico utilizada no tratamento para a leucemia linfoblástica aguda (LLA) devido à sua capacidade de hidrolisar a L-asparagina em L-aspartato e amônia (Gurunathan e Sahadevan, 2012; Nomme et al., 2012). As preparações dessa enzima para uso clínico são derivadas de fonte bacteriana e a sua utiliza...
Article
Biomass represents an abundant and inexpensive source of sugars and aromatic compounds that can be used as raw materials for conversion into value-added bioproducts. Filamentous fungi are sources of plant cell wall degrading enzymes in nature. Understanding the interactions between enzymes is crucial for optimizing biomass degradation processes. He...
Article
Full-text available
The enzyme L-asparaginase (L-ASNase) is used in the treatment of Acute Lymphoblastic Leukemia. The preparations of this enzyme for clinical use are derived from bacterial sources and its use is associated with serious adverse reactions. In this context, it is important to find new sources of L-ASNase. In this work, the Placket-Burman Experimental D...
Article
Full-text available
l-asparaginase is an enzyme used as treatment for acute lymphoblastic leukemia (ALL) due to its ability to hydrolyze l-asparagine, an essential amino acid synthesized by normal cells unlike neoplastic cells. The adverse effects of l-asparaginase formulations are associated with its glutaminase activity and bacterial origin; therefore, it is importa...
Article
Interest in lignocellulosic biomass conversion technologies has increased recently because of their potential to reduce the dependency on non-renewable feedstocks. Residues from a variety of crops are the major source of lignocellulose, which is being produced in increasingly large quantities worldwide. The commercial exploitation of crop residues...
Article
Full-text available
Biorefineries are core facilities for implementing a sustainable circular bioeconomy. These facilities rely on microbial enzymes to hydrolyze lignocellulosic substrates into fermentable sugars. Fungal co-cultures mimic the process of natural biodegradation and have been shown to increase certain enzyme activities. Trichoderma reesei and its many mu...
Article
Full-text available
Given the global abundance of plant biomass residues, potential exists in biorefinery-based applications with lignocellulolytic fungi. Frequently isolated from agricultural cellulosic materials, Aspergillus terreus is a fungus efficient in secretion of commercial enzymes such as cellulases, xylanases and phytases. In the context of biomass sacchari...
Article
Aspergillus terreus can produce different holocellulose‐degrading enzymes when grown in sugarcane bagasse, with predominant pectinase activity. Thus, pectinase was selected for purification and immobilization studies. Ion exchange and molecular exclusion chromatography studies were performed, after which it was possible to semi‐purify the enzyme wi...
Article
Full-text available
Aspergillus tamarii grows abundantly in naturally composting waste fibers of the textile industry and has a great potential in biomass decomposition. Amongst the key (hemi)cellulose-active enzymes in the secretomes of biomass-degrading fungi are the lytic polysaccharide monooxygenases (LPMOs). By catalyzing oxidative cleavage of glycoside bonds, LP...
Article
Full-text available
Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. the conversion of lignin into high value-added fuels, chemicals, or materials, is crucial for the full implementation of cost-effective lignocellulosic biorefineries. From this perspective, rapid scree...
Article
L-asparaginase has been used in the remission of malignant neoplasms such as acute lymphoblastic leukemia. The search for new sources of this enzyme has become attractive for therapeutics. Traditional methods for biomolecule purification involve several steps. A two-phase system may be a good strategy to anticipate one of these stages. This study a...
Article
Clonostachys byssicola is a filamentous fungus with biotechnological potential for holocellulases production. The present work describes a qualitative and quantitative proteome analysis of C. byssicola secretome cultivated in media supplemented with commercial (Avicel, carboxymethylcellulose or oat spelt xylan) or agricultural (corn cob, soybean hu...
Chapter
Two oil crises in the 1970s brought oil prices to record levels. Since then, the need for alternatives to petroleum-based fuels and chemicals has been recognized. In this sense a bio-based economy has been promoted in the format of sustainable biorefineries. As defined by the National Renewable Energy Laboratory (NREL; CO, United States) and Intern...
Article
Lowering the price of enzymes is one of the key challenges to stablish the full potential of lignocellulosic biorefineries. Lignocellulolytic enzymes are used to deconstruct the complex plant cell wall, releasing fermentable sugars that can be further used in the production of lignocellulosic ethanol. Countries like Brazil, India, and China are alr...
Article
Holocellulase production by Aspergillus niger using raw sugarcane bagasse (rSCB) as the enzyme-inducing substrate is hampered by the intrinsic recalcitrance of this material. Here we report that mild hydrothermal pretreatment of rSCB increases holocellulase secretion by A. niger. Quantitative proteomic analysis revealed that pretreated solids (PS)...
Article
Full-text available
β-Galactosidases are widely used for industrial applications. These enzymes could be used in reactions of lactose hydrolysis and transgalactosylation. The objective of this study was the production, purification, and characterization of an extracellular β-galactosidase from a filamentous fungus, Aspergillus niger. The enzyme production was optimize...
Chapter
The use of ion-exchange materials for separation and purification of proteins is a widely studied and applied tool since the twentieth century. Following the same basic principle of charge interactions, new applications for ion-exchange materials have emerged in recent decades. Enzymatic immobilization technology is one of the most promising applic...
Article
Full-text available
Heteropolymers of mannan are polysaccharide components of the plant cell wall of gymnosperms and some angiosperms, including palm trees (Arecales and Monocot). Degradation of the complex structure of these polysaccharides requires the synergistic action of enzymes that disrupt the internal carbon skeleton of mannan and accessory enzymes that remove...
Article
Full-text available
The production of bioethanol from non-food agricultural residues represents an alternative energy source to fossil fuels for incorporation into the world's economy. Within the context of bioconversion of plant biomass into renewable energy using improved enzymatic cocktails, Illumina RNA-seq transcriptome profiling was conducted on a strain of Aspe...
Article
A 22 kDa xylanase (AtXyl1) from Aspergillus tamarii was purified by two chromatographic steps and presented preference for oat spelt (OSX), birchwood (BrX) and beechwood (BeX) xylans respectively, as substrates. AtXyl1 displays the highest activity at pH 5.5 and 55 °C and showed tolerance over a range of different phenolic compounds. The activity o...
Article
Full-text available
The aim of this study was to valorize the hemicellulose-rich liquid fraction (liquor) arising from hydrothermal pretreatment of sugarcane bagasse (SCB) through its utilization as an unconventional, soluble carbon source for the production of hemicellulases, namely xylanases and α-L-arabinofuranosidases (ABFases), by Aspergillus niger DCFS11. Throug...
Article
Alternative energy sources have received increasing attention in recent years. The possibility of adding value to agricultural wastes, by producing biofuels and other products with economic value from lignocellulosic biomass by enzymatic hydrolysis, has been widely explored. Lignocellulosic biomass, as well as being an abundant residue, is a comple...
Article
Full-text available
Enzymatic hydrolysis is an important but expensive step in the process to obtain enzyme derived products. Thus, the production of efficient enzymes is of great interest for this biotechnological application. The production of xylanase by Aspergillus foetidus in soybean residues was optimized using 2×23 factorial designs. The experimental data was f...
Chapter
The development of food processing techniques is intertwined with the history of mankind. Some processes, such as brewing, are dated from the dawn of civilization. In this context, the use of biological agents has been of utmost importance for both the improvement of nutritional and sensorial characteristics of foods or development of new products....
Article
Recent decades have seen the growth of immense interest in lignocellulosic biomass conversion technologies. This interest is motivated by their huge potential for energy and bioproduct generation and reduced dependency on non-renewable feedstocks, leading to improved air quality and reduced emission of greenhouse gases. It is in this context that t...
Article
Full-text available
The present study describes the one-step purification and biochemical characterization of an endo-1,4-β-xylanase from Aspergillus tamarii Kita. Extracellular xylanase was purified to homogeneity 7.43-fold through CM-cellulose. Enzyme molecular weight and pI were estimated to be 19.5kDa and 8.5, respectively. The highest activity of the xylanase was...
Article
Full-text available
The economic viability of enzyme-based lignocellulosic biomass biorefineries depends on the low cost of holocellulose-degrading enzymes necessary for decomposing biomass into fermentable sugars and other value-added products. The high costs of commercial enzymes and the high enzyme loadings required for biomass hydrolysis motivates the use of ligno...
Article
Multienzymatic complexes with plant lignocellulose-degrading activities have recently been identified in filamentous fungi secretomes. Such complexes have potential biotechnological applications in the degradation of agro-industrial residues. Fungal species from the Clonostachys genus have been intensively investigated as biocontrol agents; however...
Article
Fungal lytic polysaccharide monooxygenases (LPMOs) from family AA9 are oxidative enzymes that, in the past few years, have changed the paradigm of cellulose conversion. They are key factor in the lignocellulose breakdown and are widely distributed among fungi. This review focuses on LPMOs from family AA9 and gives an overview of recent discoveries...
Article
An acidic thermostable protease was extracellularly produced either in shake flask or in stirred tank bioreactor by an Aspergillus foetidus strain isolated from the Brazilian savanna soil using different nitrogen sources. Its maximum activity (63.7 U mL⁻¹) was obtained in a medium containing 2% (w/v) peptone. A cultivation carried out in a 5.0 L st...
Chapter
Lignocellulose represents one of the main sources of renewable energy for biofuel production. Within this context, sugarcane bagasse, which is a discarded by-product of sugarcane processing, is a rich source of lignin, hemicellulose, and cellulose. Since sugarcane bagasse is a complex lignocellulosic structure, its complete enzymatic hydrolysis req...
Book
The continuous innovation of molecular and information technologies and the expansion of research into omics dramatically transform the landscape of microbial technology. This book aims to provide a consolidated overview of the research developments within this complex and multidisciplinary topic; encompassing the latest advances and future prospec...
Chapter
This chapter overviews the cellulose-degrading enzymes produced by microorganisms and their utility in the textile industry. The cellulose-degrading enzyme system is composed of cellobiohydrolases I and II (EC 3.2.1.76 and EC 3.2.1.91), endo-1,4-β-glucanase (EC 3.2.1.4), and β-glucosidase (EC 3.2.1.21), acting in synergy for the conversion of cellu...
Conference Paper
Full-text available
L-Asparaginase is an important chemotherapeutic agent for management of acute lymphoblastic leukemia. The enzyme catalyzes the hydrolysis of L-asparagine to L-aspartate. Microorganisms have proved to be very efficient and inexpensive sources of this enzyme. Currently, the only enzymes available for use in the clinic are of bacterial origin, however...
Article
Aspergillus tamarii was cultivated in different textile wastes. Xylanases with high levels of enzymatic activity were produced from the second day of cultivation, with constant production for up to seven days. Two xylanases, Xyl-1 and Xyl-2, with a molecular mass of 35.5 and 22 kDa respectively, were isolated from the crude extract and purified by...
Article
Enzymes are biocatalysts with huge potential for industry because they have specific catalytic properties, are easy to produce, and are environmentally friendly. These biocatalysts are remarkable molecules for use in the field of bioprocessing technology. However, for certain biocatalytic processes, native enzymes do not meet the requirements for l...
Article
Full-text available
Hemicelluloses are a vast group of complex, non-cellulosic heteropolysaccharides that are classified according to the principal monosaccharides present in its structure. Xylan is the most abundant hemicellulose found in lignocellulosic biomass. In the current trend of a more effective utilization of lignocellulosic biomass and developments of envir...
Article
Lignocellulose, the most abundant renewable carbon source on earth, is the logical candidate to replace fossil carbon as the major biofuel raw material. Nevertheless, the technologies needed to convert lignocellulose into soluble products that can then be utilized by the chemical or fuel industries face several challenges. Enzymatic hydrolysis is o...
Article
An extracellular pectinase (PEC-I) was isolated from the crude extract of Aspergillus oryzae when grown on passion fruit peel (PFP) as the carbon source and partially purified by ultra filtration, gel filtration and ion-exchange chromatography procedures. Pectinase activity was predominantly found in the retentate. The pectinase from retentate (PEC...
Article
Full-text available
An extracellular beta-mannanase was isolated from samples of crude extract of the mesophilic fungus Aspergillus foetidus grown on soybean husk as a carbon source. The induction profile showed that beta-mannanase reached a maximum activity level (2.0 IU/mL) on the 15th day of cultivation. The enzyme was partially purified by ultrafiltration and gel...
Article
The kinetics of a thermostable extracellular acid protease produced by an Aspergillus foetidus strain was investigated at different pH, temperatures and substrate concentrations. The enzyme exhibited maximal activity at pH 5.0 and 55°C, and its irreversible deactivation was well described by first-order kinetics. When temperature was raised from 55...
Article
Full-text available
The fungus Trichoderma reesei is a major producer of plant cell wall-degrading enzymes, such as cellulases and hemicellulases. These enzymes have been used to hydrolyze agro-industrial wastes in processes of second-generation bio-fuel production. The disaccharide lactose, the main by-product of dairy industry, is considered the most attractive subs...
Article
Full-text available
An endo-β-1,4-xylanase (X22) was purified from crude extract of Emericella nidulans when cultivated on submerged fermentation using sugarcane bagasse as the carbon source. The purified protein was identified by mass spectrometry and was most active at pH and temperature intervals of 5.0-6.5 and 50-60°C, respectively. The enzyme showed half-lives of...
Article
Full-text available
Proteases hydrolyze the peptide bonds of proteins into peptides and amino acids, being found in all living organisms, and are essential for cell growth and differentiation. Proteolytic enzymes have potential application in a wide number of industrial processes such as food, laundry detergent and pharmaceutical. Proteases from microbial sources have...
Chapter
Full-text available
The commercial enzyme market is a dynamic one that is forecast to show significant growth over the next decade. Enzymes are being used in a diverse array of applications in industries, ranging from the food, feed, detergent, paper, leather, biofuel and textile industries, and new areas of application are constantly added. Within this scenario of en...
Article
The partitioning of protease expressed by Penicillium fellutanum from Brazilian Savanna in a novel inexpensive and stable aqueous two-phase system (ATPS) composed of poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) was studied in this work using factorial design. The aqueous two-phase systems are formed by mixing both polymers with a salt...
Article
The present work aims at characterizing T. harzianum secretome when the fungus is grown in synthetic medium supplemented with one of the four substrates: glucose, cellulose, xylan and sugarcane bagasse (SB). The characterization was done by enzymatic assays and proteomic analysis using 2-DE/MALDI-TOF and gel-free shotgun LC-MS/MS. The results showe...
Article
Filamentous fungi secrete a large amount of cellulose-degrading enzymes. Among them, fungi from Trichoderma genus are described as producers and secretors of a wide spectrum of cellulose-degrading enzymes. The biological deconstruction of cellulose within the biomass up to glucose monom is achieved through the synergistic action of multiple enzymes...
Article
Full-text available
The filamentous fungus Acrophialophora nainiana, isolated from a hot spring in Brazil, was grown in liquid culture on different cellulosic and lignocellulosic carbon sources for seven days and enzyme extracts were characterised with respect to their carbohydrase activity profile. The enzyme extracts obtained from growing A. nainiana on cellulose, d...
Article
Full-text available
Sugarcane bagasse was used as an inexpensive alternative carbon source for production of β-xylanases from Aspergillus terreus. The induction profile showed that the xylanase activity was detected from the sixth day of cultivation period. Two low molecular weight enzymes, named Xyl T1 and Xyl T2 were purified to apparent homogeneity by ultrafiltrati...
Article
Full-text available
Aspergillus nidulans is poorly exploited as a source of enzymes for lignocellulosic residues degradation for biotechnological purposes. This work describes the A. nidulans Endoglucanase A heterologous expression in Pichia pastoris, the purification and biochemical characterization of the recombinant enzyme. Active recombinant endoglucanase A (rEG A...
Chapter
The global enzyme market was estimated at US $5 billion in 2009. Taking into consideration the compound annual growth rate (CAGR) of 6 % for the next 5 years, this market is expected to reach US $7 billion by 2015. Enzymes have been used in a wide range of applications in the fuel, pharmaceutical, brewing, food, animal feed, bioremediation, deterge...
Article
Full-text available
Pretreated dirty cotton residue (PDCR) from the textile industry was used as an alternative carbon source for the submerged cultivation of Aspergillus oryzae and the production of xylanases. The filtered culture supernatant was fractionated by ultrafiltration followed by three chromatographic steps, which resulted in the isolation of a homogeneous...
Article
Plant cell wall-degrading enzymes produced by microorganisms possess important biotechnological applications, including biofuel production. Some anaerobic bacteria are able to produce multienzymatic complexes called cellulosomes while filamentous fungi normally secrete individual hydrolytic enzymes that act synergistically for polysaccharide degrad...
Article
Trichoderma harzianum is a mycoparasitic filamentous fungus that produces and secretes a wide range of extracellular hydrolytic enzymes used in cell wall degradation. Due to its potential in biomass conversion, T. harzianum draws great attention from biofuel and biocontrol industries and research. Here, we report an extensive secretome analysis of...
Article
Full-text available
Aspergillus niger van Tieghem LPM 93 was shown in an earlier study to produce the most thermostable β-xylanase, which was effective for improving brightness and delignification of non-delignified and oxygen-bleached samples of eucalyptus kraft pulp. Here, we report the production, purification, and characterization of a xylan-degrading enzyme (XynI...
Article
Full-text available
Holocellulose structures from agro-industrial residues rely on main and side chain attacking enzymes with different specificities for complete hydrolysis. Combinations of crude enzymatic extracts from different fungal species, including Aspergillus terreus, Aspergillus oryzae, Aspergillus niger and Trichoderma longibrachiatum, were applied to sugar...