
Edet Effiong AsangaArthur Jarvis University, Akpabuyo, Cross River State, Nigeria · Biochemistry
Edet Effiong Asanga
Ph.D, AISLT, MNSBMB
The application of computational drug design models with the various biochemical validations to discover drugs/targets
About
48
Publications
14,995
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
341
Citations
Introduction
Edet Asanga Ph.D is an academic who is highly prolific in researches geared towards the discoveries of new drug targets of natural products and metabolites as well as the mechanism of regulations of targets using biochemistry, structural biology, and medicinal chemistry. Moreover, he has carried out and published many SCI papers in the fields of toxicology, public health, pharmacological and clinical biochemistry.
Education
November 2014 - February 2019
University of Calabar, Nigeria
Field of study
- Pharmacological biochemistry
October 2009 - January 2013
October 2003 - August 2006
Publications
Publications (48)
Background The high prevalence rate of malaria due to the proliferation of drug-resistant strains of Plasmodium falciparum could mitigate the realization of sustainable development goals in Nigeria, necessitating research on the discovery
of novel chemotherapies against resistant strains of the parasite. However, Solanum anomalum with ethnopharmac...
Background
Scientific and ethnopharmacological studies have shown that Justicia insularis leaves are used in the treatment of oxidative stress, heart problems, labour pains, and dysmenorrhoea. Therefore, this study was geared towards the investigation of the antioxidant claims of this plant leaves as well as the isolation and characterization of th...
This paper introduces a novel hybrid activation function by combining five popular activation functions (Sigmoid, ReLU, Tanh, Leaky ReLU, and ELU) with adjustable coefficients. This integrated approach aims to mitigate the limitations of individual functions, such as vanishing gradients and inactive neurons. Our analysis, including mathematical der...
Doxorubicin, as an antibiotic causes toxicity in human tissues through the generation of oxidant species; however, Solanum anomalum (Solanaceae) is ethnopharmacologically and scientifically reported to possess antidotal activities. This study was designed to validate the antidotal potency of the plant’s bioactive compounds on rats’ testes following...
Background: Justicia insularis (Acanthaceae) T. Anderson is ethnopharmacologically used in Nigeria for the treatment of diseases including malaria. Therefore, this study was designed to investigate in vivo antiplasmodial effect of J. insularis leaf in Plasmodium berghei-infected mice, characterize its constituents, and carryout in silico studies of...
Background
Inflammations, oxidative stress, and pains underlie the pathogenesis of non-communicable diseases (NCDs) like respiratory disorders and cancer; however, they are scientifically treated with Curcuma longa. Therefore, the pharmacological screening (antioxidant, anti-inflammatory, and analgesic) of the plant's rhizome, isolation and charact...
Plasmodium parasite causes malaria and affects the biochemical, physiological, and histoarchitecture of the hepatocytes and blood. The resultant effect leads to alterations in the metabolic activities of the liver, erythrocytes, as well as the buffer system. Therefore, we investigated the antiplasmodial activity, histomorphological studies of the h...
This study investigated the effect of ethanol extract of A. cordifolia leaves on high-fat diet (HFD)-induced obesity and its associated metabolic abnormalities in rats. Thirty male rats were randomly divided into five groups (Six rats each)- Normal control, Obese control, Orlistat group, A.cordifolia groups (500 and 1000 mg/kg body weight). Dietary...
Background
Chemotherapies target the PfEMP-1 and PfPKG proteins in Plasmodium falciparum, the parasite that causes malaria, in an effort to prevent the disease’s high fatality rate. This work identified the phytochemical components of Nauclea latifolia roots and docked the chemical compounds against target proteins, and examined the in vivo antipla...
With the rising worries of potential metal contamination in cooked noodles, this study aimed at unravelling, for the first time, the possible source of metal contamination in cooked noodles. Noodles cooked with full spices (CWFS), cooked with half spices (CWHS), cooked with quarter spices (CWQS), and cooked with no spices (CWNS: control) and their...
Background
Malaria caused by Plasmodium falciparum with an average prevalence of 75% in Nigeria, can be treated. Chemical-based medications, one of the methods used to treat it, frequently causes hematological abnormalities in the blood tissues. However, the ethnobotanical and scientific usage of Mangifera indica for the management of malaria in Ni...
Poor handling of potable water is a wellknown route of contamination. Despite this, Nigeria’s most consumed packaged potable water called “sachet” or “pure water” is still poorly handled with long hours of exposure to sunlight which may
affect its quality. This study examined the potential deterioration of the sachet water quality (potential toxic...
Background:Herbal therapies mediate similar functions with those of some well-known drugs. Ginger and aspirin are herbal and drug therapies respectively which are used for the treatment of anti-inflammatory conditions. Non-steroidal medicinal drugs especially aspirin and acetaminophen are usually linked to drug-induced liver injury. Therefore, this...
Purpose: To investigate the in vivo antiplasmodial effect of Mangifera indica aqueous extract in Plasmodium berghei-infected mice, and to characterize its phytochemical constituents and their mechanism of action. Methods: The plant leaves (250.71 g) and stem bark (509.34 g) were weighed, macerated in 5 L of boiled, distilled water for 72 h, and fil...
The exposure of bottled water to sunlight leaches heavy metals into the water, thereby deteriorating its quality and this informed the study. Three plastic bottle brands (n = 100 per brand) were exposed to sunlight for different durations. The leaching of contaminants was exposure duration dependent. The following ranges were recorded for temperatu...
Background
One of the main challenges of wound healing is infection with multi-drug resistant (MDR) bacteria such as Staphylococcus aureus. The spectrum of antibiotics used to treat them is declining; thus, there is a need for alternatives. Our study was designed to evaluate the antimicrobial properties of honey, its pharmacokinetics (ADMET) proper...
Background
Acquired immunodeficiency syndrome (AIDS) is an acquired defect of the cellular immunity associated with the infection by the human immunodeficiency virus (HIV). The disease has reached pandemic proportion and has been considered a public health concern. This study is aimed at analyzing the trend of HIV/AIDS research in Nigeria.
Method...
Background: Quercetin has been reported to possess anticancer activities. This study was aimed at designing some derivatives of quercetin and evaluating their binding affinities to target proteins implicated in cancer. Methodology: Derivatives of quercetin were designed with ChemDraw. The targets: Phosphodiesterases (PDEs; 1XMU), Cyclooxygenase-1 (...
Background
Staphylococcus aureus has prevailed against the majority of antibiotics currently in clinical use, making it a significant global public health problem. As a safer alternative, bioactive compounds have been explored. Annona muricata has been shown to possess antimicrobial activity. However, there are few reports on the molecular activity...
Background: Quercetin is known to exhibit antidiabetic activity in Type 2 Diabetes mellitus due to its antioxidant property. This study was aimed at designing some derivatives of quercetin and evaluating their binding affinities to target proteins implicated in diabetes mellitus.
Method: Derivatives of quercetin were designed with ChemDraw. The tar...
Purpose: To research the antiplasmodial property of aqueous extract, fractions, and residue of Nauclea latifolia roots and to isolate the components responsible for the antiplasmodial activity. Methods: Roots of N. latifolia were macerated with distilled water; the extract was obtained, successively partitioned with ethyl acetate and butanol. The e...
Purpose: To synthesize various ester derivatives of cinnamic acid and to evaluate their in vitro antidiabetic and antioxidant properties. Methods: Esters of cinnamic acid were synthesized by refluxing the parent compound (cinnamic acid) with different alcohols using concentrated sulphuric acid as a catalyst. Physicochemical analyses (solubility, bo...
Malaria is a parasitic disease that is endemic in Nigeria with one of the greatest challenges being the development of resistance by Plasmodium falciparum against chloroquine and Artemisinin combination therapy (ACT). Since, ethnobotanical studies have given insight on the use Nauclea latifolia (Pin cushion tree) to treat malaria, this study was ai...
Malaria is a parasitic disease that is endemic in Nigeria with one of the greatest challenges being the development of resistance by Plasmodium falciparum against chloroquine and Artemisinin combination therapy (ACT). Since, ethnobotanical studies have given insight on the use Nauclea latifolia (Pin cushion tree) to treat malaria, this study was ai...
Malaria is a parasitic disease that is endemic in Nigeria with one of the greatest challenges being the development of resistance by Plasmodium falciparum against chloroquine and Artemisinin combination therapy (ACT). Since, ethnobotanical studies have given insight on the use Nauclea latifolia (Pin cushion tree) to treat malaria, this study was ai...
This research was designed to understudy trace elements (zinc, magnesium and copper) implications in 120 subjects (60 sickle cell anemia patients and 60 non-sickle cell individuals) attending clinic at Bingham University Teaching Hospital, Jos, Plateau State, Nigeria. Their blood samples were collected and their sera used for the estimation of zinc...
Effects of cassava peels and poultry manure based compost and their sole applications on soil properties, growth and yield of waterleaf were investigated at the University of Calabar Teaching and Research Farm during 2013 and 2014 cropping seasons. The experiment was laid out in a randomized complete block design (RCBD) with three replications. The...
Allium cepa (onion) bulbs are used as spice in the preparation of virtually all meals in Nigeria, and some researchers had reported its medicinal values. This study was designed to estimate the anti-nutrients and mineral composition of this spice. Standard procedures were used for the analysis of antinutrients. Estimation of the various minerals wa...
Diabetes mellitus as a metabolic disorder is known to affect the metabolism of carbohydrate, lipids as well as protein. The myriad devastating symptoms arising from this endocrine disorder is of paramount concern to many scientists who believe that traditional medicine could be the solution to the many years of looking for an antidiabetic drug that...
Lipid profile status of streptozotocin induced diabetic rats treated with ethanolic leaf extract of Solenostemon monostachyus was assessed. A total of 24 rats were used for the experiments and were divided into four groups (that is, Diabetic Control (DC), Normal Control (NC), Insulin Treated (INS) and S. monostachyus (SM) treated groups), with 6 ra...
Aims: To evaluate the antioxidant components and also antioxidant activities of ten local
vegetables commonly consumed in Akwa Ibom State, Nigeria.
Place and Duration of Study: Department of Pharmaceutical and Medicinal Chemistry, Faculty of
Pharmacy, University of Uyo, Akwa Ibom State, Nigeria between April 2011 and July 2012.
Methodology: Standar...
Protective effects of the combined ethanolic leaf extracts of Gongronema latifolium (GL) and Nauclea latifolia (NL) on acetaminophen-induced liver toxicity were studied in rats in comparison with individual extract treatment and the standard drug; Silymarin.
Study Design: The design consisted of sixty rats divided into ten groups of six rats each....
The research was geared towards unraveling the prevalence of gastrointestinal parasites among 104 dogs resident in Ibiono Ibom Local Government Area, Akwa Ibom State, Nigeria by using microscopy and formol ether concentration techniques between the months of February and March, 2013. From the result, 77 dogs (74.0 %) were positive for gastrointesti...
The ethanol extract and its fractions were evaluated in the study to unravel their effects on the lipid profile of diabetic rats after daily administration for a period of two weeks. The analysis of the results showed that there were significant decreases (p < 0.05) in the HDL-cholesterol and total cholesterol levels in all the treatment groups. In...
Diabetes mellitus is associated with alteration in carbohydrate and lipid metabolism. The present study was designed to investigate the hypoglycaemic and hypolipedemic activities of ethanolic root extract of Gongronema latifolium (GL) in Streptozotocin (STZ) diabetic rats. Diabetes was induced by intraperitoneal injection of STZ (65mg/kg b.w) after...
Antidiabetic and Antihyperlipedemic properties of ethanolic root extract of
Gongronema Latifolium on STZ induced Diabetic Rat
The hypoglycemic effect of ethanol extract and fractions of Nauclea latifolium leaf was evaluated in this study. The result in normoglycemic rats showed maxima glycemic reduction of 26.4% within 4 hours in the group treated with butanol fraction (250 mg/kg) which proved efficacious than glibenclamide at p < 0.05. Also, similar advantage over gliben...
Diabetes mellitus as an endocrine disorder has over the years been treated with orthodox medicine and this have many drawbacks including undesirable side effects and high cost of long term treatment hence ethno botanicals have provided greater advantage over the earlier. The aim of this study is to determine the effect of ethanol extract and its fr...
Hematological parameters which have been implicated in diabetes
mellitus were investigated in this study. N-hexane, ethyl acetate, butanol and
methanol fractions of the ethanolic leaf extract of Nauclea latifolia were
orally administered once daily for 2 weeks to diabetic rats. The levels of
RBC, Hb, HCT, MCV, MCH, MCHC, PLT, PCT, MPV, PDW, WBC,
ly...
The hypoglycemic effect of ethanol extract and fractions of Nauclea latifolium leaf was evaluated in this study.
The result in normoglycemic rats showed maxima glycemic reduction of 26.4% within 4 hours in the group
treated with butanol fraction (250 mg/kg) which proved efficacious than glibenclamide at p < 0.05. Also, similar
advantage over gliben...
The research provided evidence on the anti-diabetic property of the root of Vernonia amygdalina rather than the hypoglycemic abilities showcased by the leaf of the same plant. A study on the RNA content of the pancreas of the diabetic animal model to elucidate the possible mechanism of action of this great anti-diabetic agent gave credence to the f...
Questions
Questions (4)
Schizont Maturation assay, isotopic assay, quantification of parasite proteins (histidine-rich protein 2 (HRP2) and lactate dehydrogenase (LDH) by ELISA) are the various models used to screen extracts for IN VITRO antiplasmodial activity in pharmacological studies. I am currently doing research in this area and I need a complete laboratory with the needed equipment and instrumentation within Nigeria where I can evaluate the in vitro antiplasmodial activity of my plant extract, fractions and purified compounds. Please who has information where this could be done?
Am presently working on activity guided extraction, isolation and identification of antiplasmodial compounds in a medicinal plant. Which the most ideal, comprehensive methodology for evaluating in vitro and in .vivo antiplasmodial activity of this plant. Moreover, can somebody help me with the methodolgy for cytotoxicity test of a compound?
Natural product is no doubt a very prospective research tool for the improvement and advances in pharmaceutical development and industrial revolution for the survival of man. However, what are the recent methodology and the various research areas with respect to natural product researches for pharmaceutical drug development?
medicinal plants notably have some anti-infective properties due to the secondary metabolites, these principles are responsible for their abilities in destroying microorganisms like bacteria and fungi in some animal models. The principles in these plants pose toxicity. Discuss?.